Protein Family Models

NFACT-R RNA binding family found found in bacteria fused to the ThiI domain as a variant of the canonical tRNA 4-thiouridylation pathway [1]. [1]. 24646681. A highly conserved family of domains related to the DNA-glycosylase fold helps predict multiple novel pathways for RNA modifications. Burroughs AM, Aravind L;. RNA Biol. 2014;11:360-372. (from Pfam)

Date:

2024-10-16

This family represents the N-terminal HUP domain in tRNA(5-methylaminomethyl-2-thiouridine)-methyltransferase which is involved in the biosynthesis of the modified nucleoside 5-methylaminomethyl-2-thiouridine present in the wobble position of some tRNAs [1]. [1]. 3298234. Transfer RNA(5-methylaminomethyl-2-thiouridine)-methyltransferase from Escherichia coli K-12 has two enzymatic activities. Hagervall TG, Edmonds CG, McCloskey JA, Bjork GR;. J Biol Chem 1987;262:8488-8495. Paper describing PDB structure 1gpm. [2]. 8548458. The crystal structure of GMP synthetase reveals a novel catalytic triad and is a structural paradigm for two enzyme families. Tesmer JJ, Klem TJ, Deras ML, Davisson VJ, Smith JL;. Nat Struct Biol 1996;3:74-86. Paper describing PDB structure 2der. [3]. 16871210. Snapshots of tRNA sulphuration via an adenylated intermediate. Numata T, Ikeuchi Y, Fukai S, Suzuki T, Nureki O;. Nature. 2006;442:419-424. Paper describing PDB structure 2vxo. [4]. 23816837. Substrate specificity and oligomerization of human GMP synthetase. Welin M, Lehtio L, Johansson A, Flodin S, Nyman T, Tresaugues L, Hammarstrom M, Graslund S, Nordlund P;. J Mol Biol. 2013;425:4323-4333. Paper describing PDB structure 3vrh. [5]. 23444054. Crystallographic and mutational studies on the tRNA thiouridine synthetase TtuA. Nakagawa H, Kuratani M, Goto-Ito S, Ito T, Katsura K, Terada T, Shirouzu M, Sekine S, Shigi N, Yokoyama S;. Proteins. 2013;81:1232-1244. Paper describing PDB structure 5mko. [6]. 28655838. Nonredox thiolation in tRNA occurring via sulfur activation by a [4Fe-4S] cluster. Arragain S, Bimai O, Legrand P, Caillat S, Ravanat JL, T. TRUNCATED at 1650 bytes (from Pfam)

Date:

2024-10-16

ThiI is required for thiazole synthesis, required for thiamine biosynthesis [1]. [1]. 9209060. Characterization of thiI, a new gene involved in thiazole biosynthesis in Salmonella typhimurium. Webb E, Claas K, Downs DM;. J Bacteriol 1997;179:4399-4402. (from Pfam)

Date:

2024-10-16

MnmA/TRMU family protein similar to tRNA-specific 2-thiouridylase MnmA that catalyzes the 2-thiolation of uridine at the wobble position (U34) of mitochondrial tRNA(Lys), tRNA(Glu) and tRNA(Gln)

Date:

2024-08-11