Protein Family Models

YihI activates the GTPase activity of Der, a 50S ribosomal subunit stability factor [1]. The stimulation is specific to Der as YihI does not stimulate the GTPase activity of Era or ObgE. The interaction of YihI with Der requires only the C-terminal 78 amino acids of YihI [1]. A yihI deletion mutant is viable and shows a shorter lag period, but the same post-lag growth rate as a wild-type strain. yihI is expressed during the lag period. Overexpression of yihI inhibits cell growth and biogenesis of the 50S ribosomal subunit [1]. YihI is an unusual, highly hydrophilic protein with an uneven distribution of charged residues, resulting in an N-terminal region with high pI and a C-terminal region with low pI [1]. [1]. 20434458. A bacterial GAP-like protein, YihI, regulating the GTPase of Der, an essential GTP-binding protein in Escherichia coli. Hwang J, Inouye M;. J Mol Biol. 2010;399:759-772. (from Pfam)

Date:

2024-10-16

Gene:

yihI

Date:

2022-02-11

GTPase-activating protein similar to YihI, which activates the GTPase activity of Der (double Era-like GTPase), an essential Escherichia coli tandem GTPase that plays a key role in 50S ribosome maturation at a later biogenesis step

Date:

2015-09-22