Protein Family Models

Members of this staphylococcal lipase family have an N-terminal YSIRK-type signal peptide that is known for targeting sortase-dependent (LPXTG motif-containing) cell wall proteins to the cross-wall of growing and dividing cells. However, YSIRK also occurs on proteins that associate with the cell surface through other means. The YSIRK region is followed by a long, variable length low-complexity region rich in Ser, Thr, Asn, Asp, Glu, and Gln, followed by the lipase domain. Genes for member of this family in Staphylococcus aureus may be given as lip1 (e.g. YP_501455.1) and lip2 (YP_498890.1), or gehA and gehB. Members of this family in Staphylococcus aureus are considered virulence factors.

Gene:

lip

Date:

2024-07-22

Many surface proteins found in Streptococcus, Staphylococcus, and related lineages share apparently homologous signal sequences. A motif resembling [YF]SIRKxxxGxxS[VIA] appears at the start of the transmembrane domain. The GxxS motif appears perfectly conserved, suggesting a specific function and not just homology. There is a strong correlation between proteins carrying this region at the N-terminus and those carrying the Gram-positive anchor domain with the LPXTG sortase processing site at the C-terminus. (from Pfam)

Date:

2024-08-14

The [YF]SIRKxxxGxxS type of signal peptide appears at the start of many proteins of Streptococcus, Staphylococcus, and Enterococcus, but not in other lineages such as Bacillus. Recent work in Staphylococcus aureus has shown that septal secretion (targeting to the crosswall in dividing cells) of the YSIRK-containing staphylococcal protein A depends on SecA, SecDF, and the lipoteichoic acid synthase LtaS, all of which co-purify when the motif is modified to YSIRKxxxGxxL to block processing.

Date:

2024-12-19

Lip2 (lipase 2), also called Geh (glycerol ester hydrolase), as described in Staphylococcus aureus, is one of two triacylglycerol lipases that are full-length homologs, both with a YSIRK-type signal peptide for targeting to the cross-wall of dividing cells, and both lacking any known sortase signal for covalent attachment to the cell surface, such as LPXTG, from the C-terminus. It is a paralog to Lip1.

Gene:

lip2

Date:

2019-01-14

esterase/lipase family protein is an alpha/beta hydrolase, such as triacylglycerol lipase that catalyzes the hydrolysis of a triacylglycerol to form the corresponding diacylglycerol and a carboxylate

Date:

2024-04-25