Protein Family Models

This family of enzymes includes pyruvate formate lyase [1], choline trimethylamine lyase [2], glycerol dehydratase [3], 4-hydroxyphenylacetate decarboxylase [4], and benzylsuccinate synthase [5]. [1]. 10504733. Structure and mechanism of the glycyl radical enzyme pyruvate formate-lyase. Becker A, Fritz-Wolf K, Kabsch W, Knappe J, Schultz S, Volker Wagner AF;. Nat Struct Biol 1999;6:969-975. [2]. 23151509. Microbial conversion of choline to trimethylamine requires a glycyl radical enzyme. Craciun S, Balskus EP;. Proc Natl Acad Sci U S A. 2012;109:21307-21312. [3]. 15096031. Insight into the mechanism of the B12-independent glycerol dehydratase from Clostridium butyricum: preliminary biochemical and structural characterization. O'Brien JR, Raynaud C, Croux C, Girbal L, Soucaille P, Lanzilotta WN;. Biochemistry. 2004;43:4635-4645. [4]. 11231288. p-Hydroxyphenylacetate decarboxylase from Clostridium difficile. A novel glycyl radical enzyme catalysing the formation of p-cresol. Selmer T, Andrei PI;. Eur J Biochem. 2001;268:1363-1372. [5]. 9632263. Biochemical and genetic characterization of benzylsuccinate synthase from Thauera aromatica: a new glycyl radical enzyme catalysing the first step in anaerobic toluene metabolism. Leuthner B, Leutwein C, Schulz H, Horth P, Haehnel W, Schiltz E, Schagger H, Heider J;. Mol Microbiol 1998;28:615-628. (from Pfam)

Date:

2024-10-16

Date:

2024-08-14

This family previously was designated pyruvate formate-lyase, but it now appears that members include the B12-independent glycerol dehydratase. Therefore, the functional definition of the family is being broadened. This family includes the PflF and PflD proteins of E. coli, described as isoforms of pyruvate-formate lyase found in a limited number additional species. PFL catalyzes the reaction pyruvate + CoA -> acetyl-CoA + formate, which is a step in the fermentation of glucose.

Date:

2021-08-31

glycyl radical protein similar to formate acetyltransferase, benzylsuccinate synthase, 4-hydroxyphenylacetate decarboxylase, and CutC choline trimethylamine-lyase

Date:

2017-03-27