Protein Family Models

This is the N-terminal domain of the Rolling Circle Replication Initiator Protein (Rep) from Geobacillus stearothermophilus. This protein acts on plasmids from family pT181 to initiate replication, recruit a helicase to the site of initiation and terminate replication after DNA synthesis. These proteins possess a unique active site and a catalytically essential metal ion is bound in a distinct manner from other rolling circle Reps [1]. [1]. 26792891. Structures of replication initiation proteins from staphylococcal antibiotic resistance plasmids reveal protein asymmetry and flexibility are necessary for replication. Carr SB, Phillips SE, Thomas CD;. Nucleic Acids Res. 2016;44:2417-2428. (from Pfam)

Date:

2024-10-16

Plasmid replication is initiated by the replication initiation factor (REP). This family represents a probable topoisomerase that makes a sequence-specific single-stranded nick in the plasmid DNA at the origin of replication. Human proteins also belong to this family, including myelin transcription factor 2 (Swiss:O15150) and cerebrin-50 (Swiss:Q16301) [2]. [1]. 2270231. Nucleotide sequence of the rep gene of staphylococcal plasmid pCW7. Balson DF, Shaw WV;. Plasmid 1990;24:74-80. [2]. 7735128. Cerebrin-50, a human cerebrospinal fluid protein whose mRNA is present in multiple tissues but predominantly expressed in the lymphoblastoid cells and the brain. Li AH, Silvestrini B, Leone MG, Giacomelli S, Cheng CY;. Biochem Mol Biol Int 1995;35:135-144. (from Pfam)

Date:

2024-10-16