Protein Family Models

This domain is part of a dehydratase domain found in diverse PKS enzymes. This domain adopts a HotDog fold. Paper describing PDB structure 2vz8. [1]. 18772430. The crystal structure of a mammalian fatty acid synthase. Maier T, Leibundgut M, Ban N;. Science. 2008;321:1315-1322. Paper describing PDB structure 3el6. [2]. 18952099. Crystal structure of the erythromycin polyketide synthase dehydratase. Keatinge-Clay A;. J Mol Biol. 2008;384:941-953. Paper describing PDB structure 3hhd. [3]. 20132826. Structure of the human fatty acid synthase KS-MAT didomain as a framework for inhibitor design. Pappenberger G, Benz J, Gsell B, Hennig M, Ruf A, Stihle M, Thoma R, Rudolph MG;. J Mol Biol. 2010;397:508-519. Paper describing PDB structure 3hrq. [4]. 19847268. Structural basis for biosynthetic programming of fungal aromatic polyketide cyclization. Crawford JM, Korman TP, Labonte JW, Vagstad AL, Hill EA, Kamari-Bidkorpeh O, Tsai SC, Townsend CA;. Nature. 2009;461:1139-1143. Paper describing PDB structure 3kg6. [5]. 20152156. Crystal structures of dehydratase domains from the curacin polyketide biosynthetic pathway. Akey DL, Razelun JR, Tehranisa J, Sherman DH, Gerwick WH, Smith JL;. Structure. 2010;18:94-105. (from Pfam)

Date:

2024-10-16

KAsynt_C_assoc represents the very C-terminus of a subset of proteins from the keto-acyl-synthetase 2 family. It is found in proteins ranging from bacteria to human. (from Pfam)

Date:

2024-08-14

This entry represents a domain found in a group of proteins mainly found in actinomycetes, including the polyketide synthase extender module SpnB from Saccharopolyspora spinosa. This domain shows a classical Rossmann fold [1]. Paper describing PDB structure 3slk. [1]. 22634636. Divergence of multimodular polyketide synthases revealed by a didomain structure. Zheng J, Gay DC, Demeler B, White MA, Keatinge-Clay AT;. Nat Chem Biol. 2012;8:615-621. (from Pfam)

Date:

2024-10-27

This entry represents the N-terminal HotDog domain of the dehydratase (DH) module of polyketide synthases [1]. Structural analysis shows these DH domains are double hotdogs in which the active site contains a histidine from the N-terminal hotdog and an aspartate from the C-terminal hotdog. Studies have uncovered that a substrate tunnel formed between the DH domains may be essential for loading substrates and unloading products [2]. [1]. 18952099. Crystal structure of the erythromycin polyketide synthase dehydratase. Keatinge-Clay A;. J Mol Biol. 2008;384:941-953. [2]. 20152156. Crystal structures of dehydratase domains from the curacin polyketide biosynthetic pathway. Akey DL, Razelun JR, Tehranisa J, Sherman DH, Gerwick WH, Smith JL;. Structure. 2010;18:94-105. (from Pfam)

Date:

2024-10-16

This domain is found in Enoyl-(Acyl carrier protein) reductases. (from Pfam)

Date:

2024-08-14

This enzymatic domain is part of bacterial polyketide synthases and catalyses the first step in the reductive modification of the beta-carbonyl centres in the growing polyketide chain. It uses NADPH to reduce the keto group to a hydroxy group [1]. [1]. 23790488. Structural and stereochemical analysis of a modular polyketide synthase ketoreductase domain required for the generation of a cis-alkene. Bonnett SA, Whicher JR, Papireddy K, Florova G, Smith JL, Reynolds KA;. Chem Biol. 2013;20:772-783. (from Pfam)

Date:

2024-10-16

The structure of beta-ketoacyl synthase is similar to that of the thiolase family (Pfam:PF00108) and also chalcone synthase. The active site of beta-ketoacyl synthase is located between the N and C-terminal domains. [1]. 9482715. Crystal structure of beta-ketoacyl-acyl carrier protein synthase II from E.coli reveals the molecular architecture of condensing enzymes. Huang W, Jia J, Edwards P, Dehesh K, Schneider G, Lindqvist Y;. EMBO J 1998;17:1183-1191. (from Pfam)

Date:

2024-10-16

This family of proteins utilise NAD as a cofactor. The proteins in this family use nucleotide-sugar substrates for a variety of chemical reactions. [1]. 9174344. Structural analysis of UDP-sugar binding to UDP-galactose 4-epimerase from Escherichia coli. Thoden JB, Hegeman AD, Wesenberg G, Chapeau MC, Frey PA, Holden HM;. Biochemistry 1997;36:6294-6304. (from Pfam)

Date:

2024-10-16

A 4'-phosphopantetheine prosthetic group is attached through a serine. This prosthetic group acts as a a 'swinging arm' for the attachment of activated fatty acid and amino-acid groups. This domain forms a four helix bundle. This family includes members not included in Prosite. The inclusion of these members is supported by sequence analysis and functional evidence. The related domain of Swiss:P19828 has the attachment serine replaced by an alanine. (from Pfam)

Date:

2024-08-14

This family contains a wide variety of dehydrogenases. [1]. 9735295. The refined crystal structure of Drosophila lebanonensis alcohol dehydrogenase at 1.9 A resolution. Benach J, Atrian S, Gonzalez-Duarte R, Ladenstein R;. J Mol Biol 1998;282:383-399. [2]. 10387002. Structure of tropinone reductase-II complexed with NADP+ and pseudotropine at 1.9 A resolution: implication for stereospecific substrate binding and catalysis. Yamashita A, Kato H, Wakatsuki S, Tomizaki T, Nakatsu T, Nakajima K, Hashimoto T, Yamada Y, Oda J;. Biochemistry 1999;38:7630-7637. (from Pfam)

Date:

2024-10-16

The structure of beta-ketoacyl synthase is similar to that of the thiolase family (Pfam:PF00108) and also chalcone synthase. The active site of beta-ketoacyl synthase is located between the N and C-terminal domains. The N-terminal domain contains most of the structures involved in dimer formation and also the active site cysteine [1]. [1]. 9482715. Crystal structure of beta-ketoacyl-acyl carrier protein synthase II from E.coli reveals the molecular architecture of condensing enzymes. Huang W, Jia J, Edwards P, Dehesh K, Schneider G, Lindqvist Y;. EMBO J 1998;17:1183-1191. (from Pfam)

Date:

2024-10-16

Date:

2024-08-14