This is the ssDNA binding domain of anti-restriction factor ArdC deployed by plasmids and phages in polyvalent proteins related to the BHD domains of XPC/Rad4 and the Tc-38 domain found in kinetoplastid minicircle binding proteins [1,2,3]. The structure of this domain is composed of three alpha-helices and a three-stranded beta-sheet that supports a long and protuberant beta-hairpin [3]. This domain is also found in the N-terminal region of the RP4 TraC1 primase [4], together with the Toprim domain (pfam:PF01751, pfam:PF13362 and pfam:PF13662). [1]. 30396152. Unexpected Evolution of Lesion-Recognition Modules in Eukaryotic NER and Kinetoplast DNA Dynamics Proteins from Bacterial Mobile Elements. Krishnan A, Burroughs AM, Iyer LM, Aravind L;. iScience. 2018;9:192-208. [2]. 28559295. Polyvalent Proteins, a Pervasive Theme in the Intergenomic Biological Conflicts of Bacteriophages and Conjugative Elements. Iyer LM, Burroughs AM, Anand S, de Souza RF, Aravind L;. J Bacteriol. 2017; [Epub ahead of print]. [3]. 32348296. ArdC, a ssDNA-binding protein with a metalloprotease domain, overpasses the recipient hsdRMS restriction system broadening conjugation host range. Gonzalez-Montes L, Del Campo I, Garcillan-Barcia MP, de la Cruz F, Moncalian G;. PLoS Genet. 2020;16:e1008750. [4]. 10686096. Antirestriction protein Ard (Type C) encoded by IncW plasmid pSa has a high similarity to the "protein transport" domain of TraC1 primase of promiscuous plasmid RP4. Belogurov AA, Delver EP, Agafonova OV, Belogurova NG, Lee LY, Kado CI;. J Mol Biol. 2000;296:969-977. (from Pfam)
GO Terms:- Molecular Function:
- single-stranded DNA binding (GO:0003697)
- Date:
- 2024-10-16