LOCUS WP_004152343 1085 aa linear BCT 01-JAN-2025
DEFINITION MULTISPECIES: type I restriction endonuclease subunit R
[Enterobacterales].
ACCESSION WP_004152343
VERSION WP_004152343.1
KEYWORDS RefSeq.
SOURCE Enterobacterales
ORGANISM Enterobacterales
Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria.
REFERENCE 1 (residues 1 to 1085)
AUTHORS Gao,Y., Cao,D., Zhu,J., Feng,H., Luo,X., Liu,S., Yan,X.X., Zhang,X.
and Gao,P.
TITLE Structural insights into assembly, operation and inhibition of a
type I restriction-modification system
JOURNAL Nat Microbiol 5 (9), 1107-1118 (2020)
PUBMED 32483229
REFERENCE 2 (residues 1 to 1085)
AUTHORS Loenen,W.A., Dryden,D.T., Raleigh,E.A. and Wilson,G.G.
TITLE Type I restriction enzymes and their relatives
JOURNAL Nucleic Acids Res 42 (1), 20-44 (2014)
PUBMED 24068554
REFERENCE 3 (residues 1 to 1085)
AUTHORS Gustafson,E.A. and Wessel,G.M.
TITLE DEAD-box helicases: posttranslational regulation and function
JOURNAL Biochem Biophys Res Commun 395 (1), 1-6 (2010)
PUBMED 20206133
REFERENCE 4 (residues 1 to 1085)
AUTHORS Roberts,R.J., Belfort,M., Bestor,T., Bhagwat,A.S., Bickle,T.A.,
Bitinaite,J., Blumenthal,R.M., Degtyarev,S.Kh., Dryden,D.T.,
Dybvig,K., Firman,K., Gromova,E.S., Gumport,R.I., Halford,S.E.,
Hattman,S., Heitman,J., Hornby,D.P., Janulaitis,A., Jeltsch,A.,
Josephsen,J., Kiss,A., Klaenhammer,T.R., Kobayashi,I., Kong,H.,
Kruger,D.H., Lacks,S., Marinus,M.G., Miyahara,M., Morgan,R.D.,
Murray,N.E., Nagaraja,V., Piekarowicz,A., Pingoud,A., Raleigh,E.,
Rao,D.N., Reich,N., Repin,V.E., Selker,E.U., Shaw,P.C., Stein,D.C.,
Stoddard,B.L., Szybalski,W., Trautner,T.A., Van Etten,J.L.,
Vitor,J.M., Wilson,G.G. and Xu,S.Y.
TITLE A nomenclature for restriction enzymes, DNA methyltransferases,
homing endonucleases and their genes
JOURNAL Nucleic Acids Res 31 (7), 1805-1812 (2003)
PUBMED 12654995
REFERENCE 5 (residues 1 to 1085)
AUTHORS Piekarowicz,A., Klyz,A., Kwiatek,A. and Stein,D.C.
TITLE Analysis of type I restriction modification systems in the
Neisseriaceae: genetic organization and properties of the gene
products
JOURNAL Mol Microbiol 41 (5), 1199-1210 (2001)
PUBMED 11555298
REFERENCE 6 (residues 1 to 1085)
AUTHORS Makovets,S., Doronina,V.A. and Murray,N.E.
TITLE Regulation of endonuclease activity by proteolysis prevents
breakage of unmodified bacterial chromosomes by type I restriction
enzymes
JOURNAL Proc Natl Acad Sci U S A 96 (17), 9757-9762 (1999)
PUBMED 10449767
REFERENCE 7 (residues 1 to 1085)
AUTHORS Murray,N.E., Daniel,A.S., Cowan,G.M. and Sharp,P.M.
TITLE Conservation of motifs within the unusually variable polypeptide
sequences of type I restriction and modification enzymes
JOURNAL Mol Microbiol 9 (1), 133-143 (1993)
PUBMED 8412658
COMMENT REFSEQ: This record represents a single, non-redundant, protein
sequence which may be annotated on many different RefSeq genomes
from the same, or different, species.
##Evidence-For-Name-Assignment-START##
Evidence Category :: Conserved Domain (CDD)
Evidence Accession :: Domain architecture ID 11427405
Evidence Source :: NCBI SPARCLE
##Evidence-For-Name-Assignment-END##
COMPLETENESS: full length.
FEATURES Location/Qualifiers
source 1..1085
/organism="Enterobacterales"
/db_xref="taxon:91347"
Protein 1..1085
/product="type I restriction endonuclease subunit R"
/EC_number="3.1.21.3"
/GO_function="GO:0005524 - ATP binding [Evidence IEA];
GO:0016887 - ATP hydrolysis activity [Evidence IEA];
GO:0003677 - DNA binding [Evidence IEA]; GO:0004386 -
helicase activity [Evidence IEA]; GO:0009035 - type I
site-specific deoxyribonuclease activity [Evidence IEA]"
/GO_process="GO:0009307 - DNA restriction-modification
system [Evidence IEA]"
/calculated_mol_wt=121732
Region 3..923
/region_name="COG0610"
/note="Type I site-specific restriction-modification
system, R (restriction) subunit and related helicases ...
[Defense mechanisms]"
/db_xref="CDD:440375"
ORIGIN
1 mqsaahqekh fqqyiidrlv eqgwklgdsk fydteravyp edleswikts gqqekwdkle
61 rlngaktlev lmdrldkale kqgtmqvlrq gfsiagcgli emteaapedk rnaavieryq
121 anilrvvpel kyhparefai dlvlfinglp vatvelktdf tqsceaamdq yrndrlpyda
181 ktkrrepllt fkrgavvhfa msdseimmat kldgentffl pfnkgrkdes gtvhagnppg
241 eikadgtqey pvayfweavc qpdawlrifh sfvyvekkdv vdiqgnwskk etlifprfhq
301 wtavnqmltd arengagmty lcdhsagsgk tstiswtahd lvklredngd svfnsviivt
361 drnvldgqlq davkqidhqf gviaaidrqk sskskskqls eallsgtpiv vvtiqtfpya
421 meaiitdkal kgknfaviid eahnsqtgst aaklqaalgm sgqgkmstmt vdelleqlqk
481 srarpdnisy faftgtpkhs tlmlfgrptd psqpasndnp pqafhlytmr qaieekfild
541 vlkgyvpykt afnlskqled skrvsgkaak ralaqwmslh ptnvtqkvqf ivehftknva
601 hrldgkakam vvtssraaai rykkafdryi eqhseygfih slvafsgkmt gkqvmhqdds
661 efkddvfivd eneefteqsm npdvqgqdlr fafdrpeyrv mlvadkfqtg fdqpklvamy
721 vdkkianhve ivqtfsrlnr tapgkdevfi idfvndpenv rqafttydkg ahidevqdln
781 vvyeikerld ehglydekdl aafkearfkt irdithtksp qhkalyaata gatalyndkm
841 kmlrdgmatw eaafekarak gdeagmksad hhqdeyaeqi kaligfksdl grfcrtysyi
901 aqlidfgdpe lenfaafakl lqkrlqheap etvdltglvl tgfdinarsd naedegetpv
961 lqpvgagggg vagdkpkfvk eiierlnslf geatpiqdqv afvnqifsia gesdvvmaqv
1021 esntreqamk gnlpgavqqa vvralsshqk latqvlksdr qgmtalvdmv ydllregkdi
1081 dlgmd
//