LOCUS NP_001336339 434 aa linear PRI 20-SEP-2024
DEFINITION PEX5-related protein isoform 8 [Homo sapiens].
ACCESSION NP_001336339 XP_016862100
VERSION NP_001336339.1
DBSOURCE REFSEQ: accession NM_001349410.2
KEYWORDS RefSeq.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (residues 1 to 434)
AUTHORS Chen,A.S., Liu,H., Wu,Y., Luo,S., Patz,E.F. Jr., Glass,C., Su,L.,
Du,M., Christiani,D.C. and Wei,Q.
TITLE Genetic variants in DDO and PEX5L in peroxisome-related pathways
predict non-small cell lung cancer survival
JOURNAL Mol Carcinog 61 (7), 619-628 (2022)
PUBMED 35502931
REMARK GeneRIF: Genetic variants in DDO and PEX5L in peroxisome-related
pathways predict non-small cell lung cancer survival.
REFERENCE 2 (residues 1 to 434)
AUTHORS Lyman,K.A., Han,Y., Heuermann,R.J., Cheng,X., Kurz,J.E.,
Lyman,R.E., Van Veldhoven,P.P. and Chetkovich,D.M.
TITLE Allostery between two binding sites in the ion channel subunit
TRIP8b confers binding specificity to HCN channels
JOURNAL J Biol Chem 292 (43), 17718-17730 (2017)
PUBMED 28887304
REMARK GeneRIF: allosteric coupling of the TRIP8b TPR domains both
promotes binding to HCN channels and limits binding to type 1
peroxisomal targeting signal substrates.
REFERENCE 3 (residues 1 to 434)
AUTHORS Bankston,J.R., DeBerg,H.A., Stoll,S. and Zagotta,W.N.
TITLE Mechanism for the inhibition of the cAMP dependence of HCN ion
channels by the auxiliary subunit TRIP8b
JOURNAL J Biol Chem 292 (43), 17794-17803 (2017)
PUBMED 28864772
REMARK GeneRIF: TRIP8b competes with a portion of the cAMP-binding site or
distorts the binding site by making interactions with the binding
pocket, thus acting predominantly as a competitive antagonist that
inhibits the cyclic-nucleotide dependence of HCN channels.
REFERENCE 4 (residues 1 to 434)
AUTHORS DeBerg,H.A., Bankston,J.R., Rosenbaum,J.C., Brzovic,P.S.,
Zagotta,W.N. and Stoll,S.
TITLE Structural mechanism for the regulation of HCN ion channels by the
accessory protein TRIP8b
JOURNAL Structure 23 (4), 734-744 (2015)
PUBMED 25800552
REFERENCE 5 (residues 1 to 434)
AUTHORS Kunze,M., Malkani,N., Maurer-Stroh,S., Wiesinger,C., Schmid,J.A.
and Berger,J.
TITLE Mechanistic insights into PTS2-mediated peroxisomal protein import:
the co-receptor PEX5L drastically increases the interaction
strength between the cargo protein and the receptor PEX7
JOURNAL J Biol Chem 290 (8), 4928-4940 (2015)
PUBMED 25538232
REMARK GeneRIF: the sequential formation of a highly stable trimeric
complex involving cargo protein, PEX7 and PEX5L stabilizes cargo
binding and is a prerequisite for PTS2-mediated peroxisomal import.
REFERENCE 6 (residues 1 to 434)
AUTHORS Han,Y., Noam,Y., Lewis,A.S., Gallagher,J.J., Wadman,W.J.,
Baram,T.Z. and Chetkovich,D.M.
TITLE Trafficking and gating of hyperpolarization-activated cyclic
nucleotide-gated channels are regulated by interaction with
tetratricopeptide repeat-containing Rab8b-interacting protein
(TRIP8b) and cyclic AMP at distinct sites
JOURNAL J Biol Chem 286 (23), 20823-20834 (2011)
PUBMED 21504900
REMARK GeneRIF: increasing cAMP levels in cells antagonized the
up-regulation of HCN1 channels mediated by a TRIP8b construct
binding the CNBD exclusively.
REFERENCE 7 (residues 1 to 434)
AUTHORS Sim,X., Ong,R.T., Suo,C., Tay,W.T., Liu,J., Ng,D.P., Boehnke,M.,
Chia,K.S., Wong,T.Y., Seielstad,M., Teo,Y.Y. and Tai,E.S.
TITLE Transferability of type 2 diabetes implicated loci in multi-ethnic
cohorts from Southeast Asia
JOURNAL PLoS Genet 7 (4), e1001363 (2011)
PUBMED 21490949
REFERENCE 8 (residues 1 to 434)
AUTHORS Zolles,G., Wenzel,D., Bildl,W., Schulte,U., Hofmann,A.,
Muller,C.S., Thumfart,J.O., Vlachos,A., Deller,T., Pfeifer,A.,
Fleischmann,B.K., Roeper,J., Fakler,B. and Klocker,N.
TITLE Association with the auxiliary subunit PEX5R/Trip8b controls
responsiveness of HCN channels to cAMP and adrenergic stimulation
JOURNAL Neuron 62 (6), 814-825 (2009)
PUBMED 19555650
REFERENCE 9 (residues 1 to 434)
AUTHORS Fransen,M., Amery,L., Hartig,A., Brees,C., Rabijns,A.,
Mannaerts,G.P. and Van Veldhoven,P.P.
TITLE Comparison of the PTS1- and Rab8b-binding properties of Pex5p and
Pex5Rp/TRIP8b
JOURNAL Biochim Biophys Acta 1783 (5), 864-873 (2008)
PUBMED 18346465
REFERENCE 10 (residues 1 to 434)
AUTHORS Amery,L., Sano,H., Mannaerts,G.P., Snider,J., Van Looy,J.,
Fransen,M. and Van Veldhoven,P.P.
TITLE Identification of PEX5p-related novel peroxisome-targeting signal 1
(PTS1)-binding proteins in mammals
JOURNAL Biochem J 357 (Pt 3), 635-646 (2001)
PUBMED 11463335
COMMENT VALIDATED REFSEQ: This record has undergone validation or
preliminary review. The reference sequence was derived from
AC092939.8 and AC007687.17.
On Mar 15, 2017 this sequence version replaced XP_016862100.1.
Transcript Variant: This variant (28), as well as variants 8 and
22-27, encodes isoform 8.
Sequence Note: The RefSeq transcript and protein were derived from
genomic sequence to make the sequence consistent with the reference
genome assembly. The genomic coordinates used for the transcript
record were based on alignments.
Publication Note: This RefSeq record includes a subset of the
publications that are available for this gene. Please see the Gene
record to access additional publications.
##Evidence-Data-START##
Transcript exon combination :: SRR1803612.245209.1 [ECO:0000332]
RNAseq introns :: single sample supports all introns
SAMEA2145743, SAMEA2157437
[ECO:0000348]
##Evidence-Data-END##
FEATURES Location/Qualifiers
source 1..434
/organism="Homo sapiens"
/db_xref="taxon:9606"
/chromosome="3"
/map="3q26.33"
Protein 1..434
/product="PEX5-related protein isoform 8"
/note="Pex5p-related protein; PEX5-related protein;
PEX5-like protein; PEX2-related protein; peroxin-5-related
protein; peroxisome biogenesis factor 5-like;
tetratricopeptide repeat-containing Rab8b-interacting
protein; HCN channel auxiliary subunit"
/calculated_mol_wt=48398
Region 62..>235
/region_name="Spy"
/note="Predicted O-linked N-acetylglucosamine transferase,
SPINDLY family [Posttranslational modification, protein
turnover, chaperones]; COG3914"
/db_xref="CDD:443119"
Site order(138..139,142..143,145,169,172..173,176..177,
179..180,203,206..207,210..211,214)
/site_type="other"
/note="putative protein binding surface [polypeptide
binding]"
/db_xref="CDD:276809"
Region 138..162
/region_name="TPR repeat"
/note="TPR repeat [structural motif]"
/db_xref="CDD:276809"
Region 164..433
/region_name="TPR"
/note="Tetratricopeptide (TPR) repeat [General function
prediction only]; COG0457"
/db_xref="CDD:440225"
Region 167..197
/region_name="TPR repeat"
/note="TPR repeat [structural motif]"
/db_xref="CDD:276809"
Region 202..230
/region_name="TPR repeat"
/note="TPR repeat [structural motif]"
/db_xref="CDD:276809"
Region 282..310
/region_name="TPR repeat"
/note="TPR repeat [structural motif]"
/db_xref="CDD:276809"
Site order(283,286..287,290..291,293,317,320..321,324..325,
327..328,351,354..355,358..359,362)
/site_type="other"
/note="putative protein binding surface [polypeptide
binding]"
/db_xref="CDD:276809"
Region 315..345
/region_name="TPR repeat"
/note="TPR repeat [structural motif]"
/db_xref="CDD:276809"
Region 350..378
/region_name="TPR repeat"
/note="TPR repeat [structural motif]"
/db_xref="CDD:276809"
CDS 1..434
/gene="PEX5L"
/gene_synonym="PEX5R; PEX5RP; PXR2; PXR2B; TRIP8b"
/coded_by="NM_001349410.2:466..1770"
/note="isoform 8 is encoded by transcript variant 28"
/db_xref="CCDS:CCDS58861.1"
/db_xref="GeneID:51555"
/db_xref="HGNC:HGNC:30024"
/db_xref="MIM:611058"
ORIGIN
1 maerkssssr tgskellwss ehrsqpelsg gksalnsesa selelvaptq arltkehrwg
61 sallsrnhsl eeeferakaa vesdtefwdk mqaeweemar rnwisenqea qnqvtisase
121 kgyyfhtenp fkdwpgafee glkrlkegdl pvtilfmeaa ilqdpgdaea wqflgitqae
181 neneqaaiva lqrclelqpn nlkalmalav sytntghqqd acdalknwik qnpkykylvk
241 skkgspgltr rmskspvdss vlegvkelyl eaahqngdmi dpdlqtglgv lfhlsgefnr
301 aidafnaalt vrpedyslwn rlgatlangd rseeaveayt raleiqpgfi rsrynlgisc
361 inlgayreav snfltalslq rksrnqqqvp hpaisgniwa alrialslmd qpelfqaanl
421 gdldvllraf nldp
//