LOCUS NR2C1_RAT 590 aa linear ROD 27-NOV-2024
DEFINITION RecName: Full=Nuclear receptor subfamily 2 group C member 1;
AltName: Full=Orphan nuclear receptor TR2; AltName: Full=Testicular
receptor 2.
ACCESSION Q8VIJ4
VERSION Q8VIJ4.1
DBSOURCE UniProtKB: locus NR2C1_RAT, accession Q8VIJ4;
class: standard.
created: Apr 14, 2009.
sequence updated: Mar 1, 2002.
annotation updated: Nov 27, 2024.
xrefs: L26398.1, AAL31316.1, CH473960.2, EDM16889.1, BC061822.1,
AAH61822.1, NP_665723.1, XP_008763438.1, XP_017450160.1
xrefs (non-sequence databases): AlphaFoldDB:Q8VIJ4, SMR:Q8VIJ4,
STRING:10116.ENSRNOP00000071901, BindingDB:Q8VIJ4,
PhosphoSitePlus:Q8VIJ4, PaxDb:10116-ENSRNOP00000009578,
Ensembl:ENSRNOT00000009578.5, Ensembl:ENSRNOP00000009578.1,
Ensembl:ENSRNOG00000006983.8, Ensembl:ENSRNOT00055009689,
Ensembl:ENSRNOP00055007494, Ensembl:ENSRNOG00055005975,
Ensembl:ENSRNOT00060008220, Ensembl:ENSRNOP00060006182,
Ensembl:ENSRNOG00060004922, Ensembl:ENSRNOT00065020082,
Ensembl:ENSRNOP00065015404, Ensembl:ENSRNOG00065012332,
GeneID:252924, KEGG:rno:252924, UCSC:RGD:3200, AGR:RGD:3200,
CTD:7181, RGD:3200, eggNOG:KOG3575, GeneTree:ENSGT00940000158165,
InParanoid:Q8VIJ4, OMA:QDQGPNK, OrthoDB:5400963at2759,
PhylomeDB:Q8VIJ4, TreeFam:TF316650, Reactome:R-RNO-383280,
PRO:PR:Q8VIJ4, Proteomes:UP000002494, Proteomes:UP000234681,
Bgee:ENSRNOG00000006983, GO:0005634, GO:0016605, GO:0003677,
GO:0001227, GO:0042826, GO:0004879, GO:0042803, GO:0000978,
GO:1990837, GO:0008270, GO:0030154, GO:0045892, GO:0000122,
GO:0048386, CDD:cd06967, CDD:cd06952, FunFam:1.10.565.10:FF:000012,
FunFam:3.30.50.10:FF:000015, Gene3D:3.30.50.10, Gene3D:1.10.565.10,
InterPro:IPR035500, InterPro:IPR048245, InterPro:IPR048246,
InterPro:IPR000536, InterPro:IPR050274, InterPro:IPR001723,
InterPro:IPR001628, InterPro:IPR013088, PANTHER:PTHR24083,
PANTHER:PTHR24083:SF49, Pfam:PF00104, Pfam:PF00105, PRINTS:PR00398,
PRINTS:PR00047, SMART:SM00430, SMART:SM00399, SUPFAM:SSF57716,
SUPFAM:SSF48508, PROSITE:PS51843, PROSITE:PS00031, PROSITE:PS51030
KEYWORDS Activator; DNA-binding; Isopeptide bond; Metal-binding; Nucleus;
Phosphoprotein; Receptor; Reference proteome; Repressor;
Transcription; Transcription regulation; Ubl conjugation; Zinc;
Zinc-finger.
SOURCE Rattus norvegicus (Norway rat)
ORGANISM Rattus norvegicus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Rattus.
REFERENCE 1 (residues 1 to 590)
AUTHORS Ideta,R., Adachi,K., Takeda,H., Chang,C., Yeh,S., Lee,Y. and Su,C.
TITLE Gene expression of the androgen repressed rat TR2 orphan receptor:
a member of steroid receptor superfamily
JOURNAL Endocr. J. 3, 277-283 (1995)
REMARK NUCLEOTIDE SEQUENCE [MRNA].;
TISSUE=Testis
REFERENCE 2 (residues 1 to 590)
AUTHORS Mural,R.J., Adams,M.D., Myers,E.W., Smith,H.O. and Venter,J.C.
TITLE Direct Submission
JOURNAL Submitted (??-SEP-2005) to the EMBL/GenBank/DDBJ databases
REMARK NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].;
STRAIN=Brown Norway
REFERENCE 3 (residues 1 to 590)
AUTHORS Gerhard,D.S., Wagner,L., Feingold,E.A., Shenmen,C.M., Grouse,L.H.,
Schuler,G., Klein,S.L., Old,S., Rasooly,R., Good,P., Guyer,M.,
Peck,A.M., Derge,J.G., Lipman,D., Collins,F.S., Jang,W., Sherry,S.,
Feolo,M., Misquitta,L., Lee,E., Rotmistrovsky,K., Greenhut,S.F.,
Schaefer,C.F., Buetow,K., Bonner,T.I., Haussler,D., Kent,J.,
Kiekhaus,M., Furey,T., Brent,M., Prange,C., Schreiber,K.,
Shapiro,N., Bhat,N.K., Hopkins,R.F., Hsie,F., Driscoll,T.,
Soares,M.B., Casavant,T.L., Scheetz,T.E., Brown-stein,M.J.,
Usdin,T.B., Toshiyuki,S., Carninci,P., Piao,Y., Dudekula,D.B.,
Ko,M.S., Kawakami,K., Suzuki,Y., Sugano,S., Gruber,C.E.,
Smith,M.R., Simmons,B., Moore,T., Waterman,R., Johnson,S.L.,
Ruan,Y., Wei,C.L., Mathavan,S., Gunaratne,P.H., Wu,J., Garcia,A.M.,
Hulyk,S.W., Fuh,E., Yuan,Y., Sneed,A., Kowis,C., Hodgson,A.,
Muzny,D.M., McPherson,J., Gibbs,R.A., Fahey,J., Helton,E.,
Ketteman,M., Madan,A., Rodrigues,S., Sanchez,A., Whiting,M.,
Madari,A., Young,A.C., Wetherby,K.D., Granite,S.J., Kwong,P.N.,
Brinkley,C.P., Pearson,R.L., Bouffard,G.G., Blakesly,R.W.,
Green,E.D., Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J.,
Myers,R.M., Butterfield,Y.S., Griffith,M., Griffith,O.L.,
Krzywinski,M.I., Liao,N., Morin,R., Palmquist,D., Petrescu,A.S.,
Skalska,U., Smailus,D.E., Stott,J.M., Schnerch,A., Schein,J.E.,
Jones,S.J., Holt,R.A., Baross,A., Marra,M.A., Clifton,S.,
Makowski,K.A., Bosak,S. and Malek,J.
CONSRTM MGC Project Team
TITLE The status, quality, and expansion of the NIH full-length cDNA
project: the Mammalian Gene Collection (MGC)
JOURNAL Genome Res 14 (10B), 2121-2127 (2004)
PUBMED 15489334
REMARK NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].;
TISSUE=Prostate
Erratum:[Genome Res. 2006 Jun;16(6):804. Morrin, Ryan [corrected to
Morin, Ryan]]
COMMENT [FUNCTION] Orphan nuclear receptor. Binds the IR7 element in the
promoter of its own gene in an autoregulatory negative feedback
mechanism. Primarily repressor of a broad range of genes including
ESR1 and RARB. Together with NR2C2, forms the core of the DRED
(direct repeat erythroid-definitive) complex that represses
embryonic and fetal globin transcription. Binds to hormone response
elements (HREs) consisting of two 5'-AGGTCA-3' half site direct
repeat consensus sequences. Also activator of OCT4 gene expression.
Plays a fundamental role in early embryogenesis and regulates
embryonic stem cell proliferation and differentiation. Mediator of
retinoic acid-regulated preadipocyte proliferation (By similarity).
{ECO:0000250}.
[SUBUNIT] Homodimer (By similarity). Heterodimer; with NR2C2 which
is required for chromatin remodeling and for binding to promoter
regions such as globin DR1 repeats (By similarity). Interacts with
ESR1; the interaction prevents homodimerization of ESR1 and
suppresses its transcriptional activity and cell growth. Interacts
with NRIP1 (via its LXXLL motifs); the interaction provides
corepressor activity. Interacts with HDAC3 (via the DNA-binding
domain); the interaction recruits phosphorylated NR2C1 to PML
bodies for sumoylation. Interacts with HDAC4 (via the DNA-binding
domain). Interacts with PIAS1; the interaction is required for
sumoylation of NR2C1. Interacts with UBE2I; the interaction is
required for sumoylation of NR2C1. Interacts with KAT2B; the
interaction acts as a corepressor of gene expression (By
similarity). {ECO:0000250}.
[SUBCELLULAR LOCATION] Nucleus
{ECO:0000255|PROSITE-ProRule:PRU00407}. Nucleus, PML body
{ECO:0000250}. Note=Recruited by HDAC3, after all-trans retinoic
acid stimulated MAPK1-mediated Thr-210 phosphorylation, to PML
bodies for subsequent sumoylation. {ECO:0000250}.
[PTM] Sumoylation requires both PIAS1 and UBE2I. Sumoylation
appears to dissociate NR2C1 from the PML nuclear bodies. Enhances
the interaction with NRIP1 but inhibits interaction with KAT2B. In
proliferating cells, stimulation by all-trans retinoic acid,
activation of MAPK1-mediated phosphorylation and recruitment to PML
bodies with subsequent sumoylation, suppresses OCT4 expression (By
similarity). {ECO:0000250}.
[PTM] Phosphorylated on several serine and threonine residues.
Phosphorylation on Thr-210, stimulated by all-trans retinoic acid
(atRA) mediates PML location and sumoylation in proliferating cells
which then modulates its association with effector molecules, KAT2B
and NRIP1. Phosphorylation on Ser-568 by PKC is important for
protein stability and function as activator of RARB (By
similarity). {ECO:0000250}.
[SIMILARITY] Belongs to the nuclear hormone receptor family. NR2
subfamily. {ECO:0000305}.
FEATURES Location/Qualifiers
source 1..590
/organism="Rattus norvegicus"
/db_xref="taxon:10116"
gene 1..590
/gene="Nr2c1"
/gene_synonym="Tr2"
Protein 1..590
/product="Nuclear receptor subfamily 2 group C member 1"
/note="Orphan nuclear receptor TR2; Testicular receptor 2"
/UniProtKB_evidence="Evidence at transcript level"
Region 1..590
/region_name="Mature chain"
/note="Nuclear receptor subfamily 2 group C member 1.
/id=PRO_0000369405."
Region 1..166
/region_name="Region of interest in the sequence"
/note="Required for interaction with KAT2B.
/evidence=ECO:0000250."
Region 96..182
/region_name="NR_DBD_TR2_like"
/note="DNA-binding domain of the TR2 and TR4 (human
testicular receptor 2 and 4) is composed of two C4-type
zinc fingers; cd06967"
/db_xref="CDD:143525"
Site 98..173
/site_type="DNA binding"
/note="Nuclear receptor.
/evidence=ECO:0000255|PROSITE-ProRule:PRU00407."
Site order(101,104,118,121,137,143,153,156)
/site_type="other"
/note="zinc binding site [ion binding]"
/db_xref="CDD:143525"
Region 101..121
/region_name="Zinc finger region"
/note="NR C4-type.
/evidence=ECO:0000255|PROSITE-ProRule:PRU00407."
Site order(111..113,119..120,122,124,127,130,150..151,154,157,
168,171)
/site_type="other"
/note="putative DNA binding site [nucleotide binding]"
/db_xref="CDD:143525"
Region 137..156
/region_name="Zinc finger region"
/note="NR C4-type.
/evidence=ECO:0000255|PROSITE-ProRule:PRU00407."
Site 185
/site_type="phosphorylation"
/note="Phosphoserine.
/evidence=ECO:0000250|UniProtKB:Q505F1."
Site 203
/site_type="phosphorylation"
/note="Phosphoserine.
/evidence=ECO:0000250|UniProtKB:P13056."
Site 208
/site_type="phosphorylation"
/note="Phosphothreonine.
/evidence=ECO:0000250|UniProtKB:P13056."
Site 210
/site_type="phosphorylation"
/note="Phosphothreonine; by MAPK1.
/evidence=ECO:0000250|UniProtKB:Q505F1."
Bond bond(238)
/bond_type="xlink"
/note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
G-Cter in SUMO); alternate. /evidence=ECO:0000250."
Bond bond(238)
/bond_type="xlink"
/note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
G-Cter in SUMO2); alternate.
/evidence=ECO:0000250|UniProtKB:P13056."
Region 333..577
/region_name="Domain"
/note="NR LBD.
/evidence=ECO:0000255|PROSITE-ProRule:PRU01189."
Region 355..576
/region_name="NR_LBD_TR2_like"
/note="The ligand binding domain of the orphan nuclear
receptors TR4 and TR2; cd06952"
/db_xref="CDD:132750"
Site order(379..380,384,418,422,425,439,466)
/site_type="other"
/note="putative ligand binding site [chemical binding]"
/db_xref="CDD:132750"
Site order(389,392,396,401,406..407,409..410,413..414)
/site_type="active"
/note="putative coactivator recognition site [active]"
/db_xref="CDD:132750"
Site 568
/site_type="phosphorylation"
/note="Phosphoserine; by PKC.
/evidence=ECO:0000250|UniProtKB:Q505F1."
Region 571..590
/region_name="Region of interest in the sequence"
/note="Required for interaction with NRIP1.
/evidence=ECO:0000250."
Bond bond(575)
/bond_type="xlink"
/note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
G-Cter in SUMO2). /evidence=ECO:0000250|UniProtKB:P13056."
ORIGIN
1 matieeiahq iidqqmgeiv teqqtgqkiq ivtaldhstq gkqfilanhe gstpgkvflt
61 tpdaagvnql ffaspdlstp hlqlltensp dqgpnkvfdl cvvcgdkasg rhygaitceg
121 ckgffkrsir knlvyscrgs kdciinkhhr nrcqycrlqr ciafgmkqds vqcerkpiev
181 srekssncaa stekiyirkd lrsplaatpt fvtdsetars tglldsgmfv nihpsgikte
241 pallmtpdka escqgdlgtl asvvtslanl gkakdlshcg gdlpvvqslr ngdtsfgafh
301 qdiqtngdvs rafdnlakal tpgenpacqs pgesmegsth liagepscme regpllsdsh
361 vvfrltmpsp mpeylnvhyi gesasrllfl smhwalsips fqalgqensi slvkaywnel
421 ftlglaqcwq vmnvatilat fvnclhnslq qdkmsperrk llmehifklq efcnsmvklc
481 idgheyaylk aivlfspdhp glenmeliek fqekayvefq dyitrtypdd tyrlsrlllr
541 lpalrlmnat iteelffkgl ignvridsvi philkmepad ynsqiighsl
//