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Potency index, ratio of m7GMP Kd to test compound Kd for inhibition of m7GDP fluorescent probe binding to recombinant eIF4E (unknown origin) assessed as dissociation constant by fluorescence polarization assay
Assay data:1 Tested
SummaryPubMed CitationRelated BioAssays by Target
Inhibition of P32-[alpha-GTP] cap radiolabelled RNA binding to recombinant eIF4E (unknown origin) assessed as decrease in cross linked RNA-eIF4E complex formation at 10 uM preincubated with UV radiation for 30 mins followed by RNase addition and measured after 30 mins by SDS-PAGE based autoradiographic analysis relative to control
Inhibition of m7 GDP fluorescent probe binding to recombinant eIF4E (unknown origin) at 10 uM by fluorescence polarization assay relative to control
Inhibition of protein synthesis in methionine-starved human MCF7 cells incubated for 24 hrs by scintillation counting based 35S-Met incorporation assay
Assay data:23 Tested
SummaryPubMed Citation
Inhibition of protein synthesis in methionine-starved human HeLa cells incubated for 24 hrs by scintillation counting based 35S-Met incorporation assay
Inhibition of eIF4E-mediated translation in luciferase RNA primed rabbit reticulocyte lysate assessed as reduction in luciferase production incubated for 90 mins by agarose gel electrophoresis
Assay data:1 Active, 23 Tested
SummaryCompounds, ActivePubMed Citation
Inhibition of P32-[alpha-GTP] cap radiolabelled RNA binding to recombinant eIF4E (unknown origin) assessed as decrease in cross linked RNA-eIF4E complex formation preincubated with UV radiation for 30 mins followed by RNase addition and measured after 30 mins by SDS-PAGE based autoradiographic analysis
Assay data:2 Active, 1 Activity ≤ 1 µM, 23 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of m7GDP fluorescent probe binding to recombinant eIF4E (unknown origin) assessed as dissociation constant by fluorescence polarization assay
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