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CYP27B1 Inhibition Assay from Article 10.1021/bi101488p: "Screening of selective inhibitors of 1a,25-dihydroxyvitamin D3 24-hydroxylase using recombinant human enzyme expressed in Escherichia coli."
Assay data:6 Active, 3 Activity ≤ 1 µM, 6 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by DepositorRelated BioAssays by Target
Inhibition of CYP27B in human SW900 cells using 3H-(23,24)-25-(OH)D3 as substrate incubated for 5 hrs by HPLC method
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of human CYP27B1 expressed in Escherichia coli assessed as reduction in hydrolase activity incubated for 25 mins using Adx, AdR and 1,25(OH)2D3
Assay data:3 Active, 3 Activity ≤ 1 µM, 3 Tested
Inhibition of human CYP27B1
Human CYP27B1 (CYP24, CYP26 and CYP27 families)
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Ratio of Kcat to Km for mouse CYP27B1 measured per mol of phospholipid after 3 mins by HPLC
Assay data:2 Tested
SummaryPubMed CitationRelated BioAssays by Target
Activity of mouse CYP27B1 assessed as compound hydroxylation measured per mol of phospholipid after 3 mins by HPLC
Substrate activity at mouse CYP27B1 assessed as 1alpha-hydroxylation measured per nmol of protein after 2 mins by reverse-phase HPLC analysis
Substrate activity at mouse CYP27B1 assessed as 1alpha-hydroxylation by measuring ratio of Kcat to Km after 2 mins by reverse-phase HPLC analysis
Substrate activity at mouse CYP27B1 assessed as 1alpha-hydroxylation measured per mol of phospholipid after 2 mins by reverse-phase HPLC analysis
Inhibition of mouse CYP27B1 by cell-free assay
Assay data:7 Active, 7 Activity ≤ 1 µM, 7 Tested
Ratio of kcat to Km for mouse CYP27B1 after 5 to 20 mins by HPLC analysis
Assay data:4 Tested
Activity of mouse CYP27B1 after 5 to 20 mins by HPLC analysis
Assay data:4 Active, 4 Activity ≤ 1 µM, 4 Tested
Inhibition of mouse CYP27B1 using 1,25(OH)2D3 substrate in presence of bovine adrenodoxin, adrenodoxin reductase and NADPH incubated at 37 degC for 25 mins by HPLC method
Assay data:12 Active, 12 Activity ≤ 1 µM, 12 Tested
Drug metabolism assessed as human CYP27B1-mediated compound metabolism after 2 to 12 mins incubation using compound loaded phospholipid vesicle by reverse phase HPLC
Assay data:1 Active, 2 Tested
Inhibition of CYP27B1
Inhibition of CYP27B1 in human keratinocytes
Inhibition of human CYP27B hydroxylase expressed in SW900 cells
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