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Inhibition of USP21 (unknown origin) using ubiquitin rhodamine 110 as substrate at 10 uM by DUBprofiler fluorometric assay
Assay data:2 Tested
SummaryPubMed CitationRelated BioAssays by Target
Inhibition of USP21 (unknown origin) using ubiquitin rhodamine 110 as substrate at 1 uM by DUBprofiler fluorometric assay
Inhibition of human USP21 using ubiquitin rhodamine 110 as substrate at 10 to 50 uM by fluorometric assay
Inhibition of full-length human USP21 transfected in human HEK293T cells co-transfected with NF-kappaB reporter firefly luciferase plasmid assessed as NF-kappaB activation and measured after 20 hrs by dual-luciferase reporter gene assay
Assay data:1 Active, 1 Activity ≤ 1 nM, 1 Activity ≤ 1 µM, 2 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Binding affinity to C-terminally NanoLuc HiBiT-tagged USP21 transfected in human HEK293T cells ssessed as thermal stability at 49 degreeC incubated for 1 hrs by CESTA analysis
Binding affinity to C-terminally NanoLuc HiBiT-tagged USP21 transfected in human HEK293T cells assessed as melting temperature at 10 uM incubated for 1 hrs by CESTA analysis
Displacement of wild-type Ubiquitin from recombinant human N-terminal His6-tagged USP21 (209 to 563 residues) expressed in Escherichia coli BL21 (DE3) by SPR competition binding assay
Assay data:1 Active, 2 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Binding affinity to C-terminally NanoLuc HiBiT-tagged USP21 transfected in human HEK293T cells assessed as increase in thermal stabilization at 49 degreeC at 50 to 100 uM incubated for 1 hrs by Nano-Glo substrate based CESTA analysis relative to control
Assay data:4 Active, 5 Tested
Inhibition of full-length human USP21 transfected in human HEK293T cells co-transfected with NF-kappaB reporter firefly luciferase plasmid assessed as NF-kappaB activation at 10 uM and measured after 20 hrs by dual-luciferase reporter gene assay
Assay data:2 Active, 4 Tested
Inhibition of human USP21 using ubiquitin rhodamine 110 as substrate assessed as maximal response preincubated for 15 to 20 mins followed by enzyme addition and measured after 25 mins by fluorometric assay
Assay data:8 Tested
Inhibition of human full length USP21 using Btn-Ahx-PNIRFLD-K(Ubi)-LPQQT-GD-amide as substrate assessed as maximal response preincubated for 15 to 20 mins followed by substrate addition and measured after 25 mins by HTRF assay
Assay data:11 Tested
Inhibition of human USP21 (209 to 563 residues) expressed in Escherichia coli BL21 (DE3) using ubiquitin rhodamine 110 as substrate assessed as remaining enzyme activity at 10 to 50 uM by fluorometric assay
Assay data:1 Tested
Binding affinity to recombinant human N-terminal His6-tagged USP21 (209 to 563 residues) expressed in Escherichia coli BL21 (DE3) assessed as dissociation constant by SPR analysis
Assay data:3 Active, 3 Activity ≤ 1 µM, 3 Tested
Binding affinity to recombinant human USP21 assessed as change in melting temperature at 100 uM by thermal shift assay
Assay data:4 Tested
Inhibition of human USP21 using ubiquitin aminoluciferin as substrate incubated for 25 mins by luminescence based assay
Inhibition of human USP21 using ubiquitin rhodamine 110 110 as substrate preincubated for 15 to 20 mins followed by enzyme addition and measured after 25 mins by fluorometric assay
Assay data:11 Active, 10 Activity ≤ 1 µM, 22 Tested
Inhibition of human full length USP21 using Btn-Ahx-PNIRFLD-K(Ubi)-LPQQT-GD-amide as substrate preincubated for 15 to 20 mins followed by substrate addition and measured after 25 mins by HTRF assay
Assay data:14 Active, 13 Activity ≤ 1 µM, 22 Tested
Inhibition of USP21 (unknown origin) at 10 uM relative to control
Inhibition of human GST-tagged USP21 CD (196 to 565 residues) expressed in Escherichia coli assessed as cleavage of Ubiquitin-Rhodamine110-glycine to Ubiquitin and Rhodamine110-glycine using Ubiquitin-Rhodamine110-glycine as substrate by fluorescence based analysis
Inhibition of human USP21 using Ub-rhodamine 110 as substrate
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