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AlphaScreen Assay from Article 10.1021/cb500956g: "Identification of a fragment-like small molecule ligand for the methyl-lysine binding protein, 53BP1."
Assay data:25 Tested
SummaryPubMed CitationRelated BioAssays by DepositorRelated BioAssays by Target
Binding affinity to recombinant human CBX7 by microarray based assay
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Inhibition of CBX7 (unknown origin) assessed as inhibition of CBX4 disruption by competitive FP assay
Assay data:4 Active, 4 Tested
Binding affinity to CBX7 (unknown origin) assessed as dissociation constant by fluorescence polarization analysis
Assay data:8 Active, 5 Activity ≤ 1 µM, 8 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of CBX7 (unknown origin) assessed as dissociation constant by isothermal titration calorimetry
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
Binding affinity to recombinant human N-terminal GST-tagged CBX7 (1 to 62 residues) expressed in Escherichia coli BL2 by SPR analysis
Assay data:15 Active, 53 Tested
Binding affinity to Cbx7 (unknown origin)
Assay data:1 Tested
SummaryPubMed CitationRelated BioAssays by Target
Inhibition of Cbx7 (unknown origin) using FITC-labeled SETDB1-K1170me3 peptide as probe by fluorescence polarization assay
Assay data:1 Active, 2 Tested
Binding affinity to Cbx7 (unknown origin) by ITC method
Assay data:3 Active, 1 Activity ≤ 1 µM, 4 Tested
Inhibition of N terminally fluorescein-tagged H3K27me3 binding to N terminallyHis6-tagged human Cbx7 expressed in Escherichia coli BL21 (DE3) by fluorescence polarization assay
Binding affinity to human C-terminal His-tagged CBX7 chromodomain (8 to 62 residues) expressed in Escherichia coli Rosetta BL21(DE3)pLysS by ITC method
Assay data:1 Active, 1 Activity ≤ 1 µM, 2 Tested
Antagonist activity at recombinant human C-terminal His-tagged CBX7 chromodomain (8 to 62 residues) expressed in Escherichia coli Rosetta BL21(DE3)pLysS using biotin-labeled H3K9me3 measured after 30 mins by AlphaScreen assay
Assay data:23 Active, 17 Activity ≤ 1 µM, 47 Tested
Binding affinity to recombinant human C-terminal His-tagged CBX7 chromodomain (8 to 62 residues) expressed in Escherichia coli Rosetta BL21(DE3)pLysS by SPR method
Assay data:3 Active, 3 Tested
Binding affinity to CBX7ChD (7 to 66 residues) (unknown origin) expressed in RIPL-BL21 (DE3)-CodonPlus competent cells assessed as enhancement of FITC-ANRIL-loopC/FITC-16mer-RNA binding to protein by fluorescence anisotropy assay
Antagonist activity at CBX7ChD (7 to 66 residues) expressed in mouse embryonic stem cells assessed as transcriptional derepression of noggin at 0.5 uM after 18 to 36 hrs by qPCR method
Antagonist activity at CBX7ChD (7 to 66 residues) expressed in mouse embryonic stem cells assessed as transcriptional derepression of Hoxd3 at 0.5 uM after 18 to 36 hrs by qPCR method
Antagonist activity at CBX7ChD (7 to 66 residues) expressed in mouse embryonic stem cells assessed as reduction of protein occupancy at Hoxd3 gene locus at 0.5 uM after 18 to 36 hrs by ChIP analysis
Antagonist activity at CBX7ChD (7 to 66 residues) expressed in mouse embryonic stem cells assessed as p16INK4a expression levels at 1 to 2 uM after 12 hrs by Western blot analysis
Antagonist activity at CBX7ChD (7 to 66 residues) expressed in mouse embryoid bodies assessed as transcriptional derepression of p16INK4a mRNA levels at 1 to 2 uM after 12 hrs by qPCR method
Antagonist activity at CBX7ChD (7 to 66 residues) expressed in human PC3 cells assessed as transcriptional derepression of p16INK4a after 12 hrs by qPCR method
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