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Fluorogenic ACC Substrate Assay from Article 10.1002/cbic.201500091: "Direct Staudinger-Phosphonite Reaction Provides Methylphosphonamidates as Inhibitors of CE4 De-N-acetylases."
Assay data:5 Tested
SummaryPubMed CitationRelated BioAssays by DepositorRelated BioAssays by Target
Substrate activity at Escherichia coli PgaB at 1 mM in presence of CoCl2 or ZnCl2 by fluorescamine assay deacetylase assay
Assay data:3 Tested
SummaryPubMed CitationRelated BioAssays by Target
Inhibition of Escherichia coli PgaB expressed in Escherichia coli BL21 (DE3) cells transformed with pET28 plasmid coding for PgaB42-655 using compound pre-incubated with 1Eq DTT and using acetoxymethyl-4-methylumbelliferone as substrate measured at 2 min intervals over 10 mins in presence of ZnCl2 by fluorescence assay
Inhibition of Escherichia coli PgaB expressed in Escherichia coli BL21 (DE3) cells transformed with pET28 plasmid coding for PgaB42-655 using compound pre-incubated with 1Eq DTT and using acetoxymethyl-4-methylumbelliferone as substrate measured at 2 min intervals over 10 mins in presence of CoCl2 by fluorescence assay
Inhibition of Escherichia coli PgaB expressed in Escherichia coli BL21 (DE3) cells transformed with pET28 plasmid coding for PgaB42-655 at 10 mM using acetoxymethyl-4-methylumbelliferone as substrate measured at 2 min intervals over 10 mins in presence of CoCl2 by fluorescence assay
Assay data:1 Tested
Substrate activity at Escherichia coli PgaB at 5 mM in presence of CoCl2 or ZnCl2 by fluorescamine assay deacetylase assay
Assay data:4 Tested
Inhibition of Escherichia coli PgaB expressed in Escherichia coli BL21 (DE3) cells transformed with pET28 plasmid coding for PgaB42-655 using acetoxymethyl-4-methylumbelliferone as substrate measured at 2 min intervals over 10 mins in presence of CoCl2 by fluorescence assay
Assay data:9 Tested
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