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Inhibition of full-length human wild-type NNMT assessed as formation of MNA at 0.5 uM using nicotinamide as substrate and SAM as cofactor preincubated for 10 mins followed by substrate addition and measured after 40 mins by by HILIC-MS analysis
Assay data:2 Active, 2 Activity ≤ 1 µM, 2 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of NNMT (unknown origin) assessed as formation of MNA at 0.5 uM using nicotinamide as substrate and SAM as cofactor by fluorescence based assay
Assay data:1 Tested
SummaryPubMed CitationRelated BioAssays by Target
Inhibition of NNMT (unknown origin) assessed as formation of MNA using nicotinamide as substrate and SAM as cofactor by fluorescence based assay
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Inhibition of recombinant human NNMT assessed as formation of MNA using nicotinamide and AdoMet as substrate preincubated for 10 mins followed by substrate addition and measured after 30 mins by UHP-HILIC-MS analysis
Inhibition of recombinant human NNMT expressed in Escherichia coli BL21 (DE3) assessed as Kobs/[I] ratio and measured after 20 mins using nicotinamide as substrate and SAM as cofactor by MTase-Glo reagent based assay
Assay data:2 Tested
Inhibition of endogenous mouse NNMT overexpressed in murine 3T3-L1 cells assessed incubated for 24 hrs by LC-MS/MS analysis
Inhibition of endogenous human NNMT overexpressed in human U2OS cells assessed incubated for 24 hrs by LC-MS/MS analysis
Inhibition of human NNMT at 30 uM preincubated for 30 mins followed by substrate, cofactor addition and measured after 60 mins using nicotinamide as substrate and SAM as cofactor by fluorescence enzymatic assay
Inhibition of mouse NNMT preincubated for 30 mins followed by substrate, cofactor addition and measured after 60 mins using nicotinamide as substrate and SAM as cofactor by fluorescence enzymatic assay
Inhibition of human NNMT preincubated for 30 mins followed by substrate, cofactor addition and measured after 60 mins using nicotinamide as substrate and SAM as cofactor by fluorescence enzymatic assay
Inhibition of recombinant human NNMT (1 to 270 residues) expressed in Escherichia coli BL21 (DE3) and measured upto 60 mins by fluorescence polarization assay
Binding affinity to recombinant human NNMT expressed in Escherichia coli BL21 (DE3) assessed as Km preincubated for 10 mins followed by NAM addition and measured after 10 mins by Michaelis-Menten analysis
Binding affinity to recombinant human NNMT SAM binding site expressed in Escherichia coli BL21 (DE3) using nicotinamide as substrate and AdoMet as cofactor preincubated for 10 mins followed by substrate addition and measured after 10 mins by UHP-HILIC-MS analysis
Assay data:1 Active, 2 Tested
Binding affinity to recombinant human NNMT expressed in Escherichia coli BL21 (DE3) assessed as Km preincubated for 10 mins followed by SAM addition and measured after 10 mins by Michaelis-Menten analysis
Uncompetitive inhibition of human NNMT assessed as Km of SAM
Uncompetitive inhibition of human NNMT assessed as Km of nicotinamide
Inhibition of human NNMT
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
Inhibition of human NNMT in human K562 cells measured after 24 hrs by quantitative mass spectrometry
Assay data:7 Active, 7 Activity ≤ 1 µM, 7 Tested
Inhibition of human NNMT measured after 20 mins by luminescence based methyltransferase assay
Assay data:15 Active, 10 Activity ≤ 1 µM, 44 Tested
Inhibition of N-terminal 6His-tagged full length human recombinant NNMT (1 to 264 residues) expressed in Escherichia coli BL21 (DE3) cells using S-adenosyl-L-methionine and NAM as substrate incubated for 2 hrs by by RapidFire Mass Spectrometry
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