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Inhibition of recombinant 17beta-HSD14 (unknown origin) expressed in Escherichia coli BL21 pLysS strain at 2 uM using E2 as substrate in presence of NAD measured continuously for 15 mins by fluorimetric method
Assay data:1 Tested
SummaryPubMed CitationRelated BioAssays by Target
Inhibition of recombinant 17beta-HSD14 (unknown origin) expressed in Escherichia coli BL21 pLysS strain at 100 uM using E2 as substrate in presence of NAD measured continuously for 15 mins by fluorimetric method
Binding affinity to recombinant 17beta-HSD14 (unknown origin) expressed in Escherichia coli BL21 pLysS strain using E2 as substrate in presence of NAD measured continuously for 15 mins by fluorimetric method
Assay data:17 Active, 16 Activity ≤ 1 µM, 17 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of N-terminal 6His-tagged human human HSD17B14 expressed in Escherichia coli BL21 (DE3) pLysS at 22.2 uM using E2 substrate and NAD+ incubated for 2 hrs using purified enzyme by fluorimetric assay
Inhibition of N-terminal 6His-tagged human human HSD17B14 expressed in Escherichia coli BL21 (DE3) pLysS using E2 substrate and NAD+ incubated for 2 hrs in absence of 0.5% v/v Tween 20 using purified enzyme by fluorimetric assay
Assay data:31 Active, 31 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Inhibition of N-terminal 6His-tagged human human HSD17B14 expressed in Escherichia coli BL21 (DE3) pLysS using E2 substrate and NAD+ incubated for 2 hrs in presence of 0.5% v/v Tween 20 using purified enzyme by fluorimetric assay
Inhibition of N-terminal 6His-tagged human human HSD17B14 expressed in Escherichia coli BL21 (DE3) pLysS using E2 substrate and NAD+ incubated for 2 hrs using purified enzyme by fluorimetric assay
Assay data:31 Active, 30 Activity ≤ 1 µM, 32 Tested
Inhibition of N-terminal 6His-tagged human human HSD17B14 expressed in Escherichia coli BL21 (DE3) pLysS at 2 uM using [3H]-E2 substrate and NAD+ incubated for 2 hrs using purified enzyme by fluorimetric assay
Assay data:32 Tested
Inhibition of N-terminal 6His-tagged human human HSD17B14 expressed in Escherichia coli BL21 (DE3) pLysS at 100 uM uM using E2 substrate and NAD+ incubated for 2 hrs using purified enzyme by fluorimetric assay
Inhibition of N-terminal 6His-tagged human human HSD17B14 expressed in Escherichia coli BL21 (DE3) pLysS at 1 uM using [3H]-E2 substrate and NAD+ incubated for 2 hrs using bacterial suspension by HPLC based radioactive displacement assay
Assay data:4 Tested
Binding affinity to human 17-beta-HSD14 assessed as change in melting temperature at 0.25 mM by thermal shift assay
Assay data:3 Tested
Inhibition of human 17-beta-HSD14 using estradiol as substrate after 15 mins by fluorimetric assay
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
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