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Binding affinity to katanin p60 in human NCI-H1975 cells using biotin labelled compound incubated for 16 hrs by streptavidin based immunoprecipitation method
Assay data:2 Active, 3 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Inhibition of Katanin p60 degradation in human A549 cells preincubated for 15 mins followed by compound addition in presence of cycloheximide by immunoblotting analysis
Assay data:1 Tested
SummaryPubMed CitationRelated BioAssays by Target
Binding affinity to katanin p60 in human A549 cells incubated for 24 hrs and measured after 1 hr by streptavidin based pull down assay
Assay data:1 Active, 2 Tested
Binding affinity to katanin p60 subunit in human NCI-H1975 cell lysate using biotin-labeled compound preincubated for 24 hrs followed by streptavidin-conjugated beads addition measured after 2 hrs by immunoblot analysis
Induction of katanin p60 subunit-mediated microtubule fragmentation in human NCI-H1975 cells assessed as alpha-tubulin level in soluble form at 300 nM after 12 hrs by gel filtration chromatography/immunoblot analysis
Assay data:1 Active, 1 Tested
Induction of katanin p60 subunit-mediated microtubule fragmentation in human NCI-H1975 cells assessed as alpha-tubulin level in polymer form at 600 nM after 1 hr by immunoblot analysis (Rvb = 63%)
Induction of katanin p60 subunit-mediated microtubule fragmentation in human NCI-H1975 cells assessed as alpha-tubulin level in soluble form at 600 nM after 1 hr by immunoblot analysis (Rvb = 37%)
Induction of katanin p60 subunit-mediated microtubule fragmentation in human NCI-H1975 cells assessed as alpha-tubulin level in polymer form at 300 nM after 1 hr by immunoblot analysis (Rvb = 63%)
Induction of katanin p60 subunit-mediated microtubule fragmentation in human NCI-H1975 cells assessed as alpha-tubulin level in soluble form at 300 nM after 1 hr by immunoblot analysis (Rvb = 37%)
Induction of katanin p60 subunit-mediated microtubule fragmentation in human NCI-H1975 cells at 300 nM after 12 hrs by DAPI staining based confocal laser microscopy
Induction of katanin p60 subunit-mediated JNK signaling pathway activation assessed as increase in c-Jun phosphorylation level at S63 residues in human NCI-H1975 cells at 300 nM after 6 to 12 hrs by immunoblot analysis
Induction of katanin p60 subunit-mediated JNK signaling pathway activation assessed as increase in phosphorylation level at T183/Y185 residues in human NCI-H1975 cells at 300 nM after 6 to 12 hrs by immunoblot analysis
Induction of katanin p60 subunit-mediated JNK signaling pathway activation in human NCI-H1975 cells assessed as increase in c-Jun phosphorylation at S63 residues at 300 nM after 9 hrs by phospho-kinase antibody array method
Induction of katanin p60 subunit-mediated JNK signaling pathway activation in human NCI-H1975 cells assessed as increase in JNK phosphorylation at T183/Y185 and T221/Y223 residues at 300 nM after 9 hrs by phospho-kinase antibody array method
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