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Inhibition of tubulin polymerization in human HeLa cells assessed as appearance of rounded microtubule at 3 uM after 24 hrs by DAPI staining based confocal microscopic analysis
Assay data:2 Active, 2 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Inhibition of paclitaxel binding to tubulin (unknown origin) by ITC method
Assay data:1 Active, 1 Tested
Inhibition of colchicine binding to tubulin (unknown origin) by ITC method
Assay data:1 Tested
SummaryPubMed CitationRelated BioAssays by Target
Binding affinity to tubulin (unknown origin) by ITC method
Assay data:1 Active, 1 Activity ≤ 1 µM, 18 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Binding affinity to tubulin (unknown origin) assessed as tryptophan fluorescence quenching after 10 mins by fluorescence spectroscopic method
Inhibition of tubulin polymerization (unknown origin) preincubated for 10 mins followed by GTP addition measured after 20 mins by Coomassie blue staining based SDS-PAGE analysis
Assay data:4 Active, 3 Activity ≤ 1 µM, 21 Tested
Inhibition of tubulin polymerization in human HeLa cells assessed as fragmented or multipolar spindles formation at 20 nM by DAPI staining based immunofluorescence analysis
Inhibition of tubulin polymerization in human HeLa cells assessed as formation of sparse tubulin foci at >= 50 nM by DAPI staining based immunofluorescence analysis
Inhibition of tubulin polymerization in human HeLa cells assessed as microtubule asters formation at 100 nM by DAPI staining based immunofluorescence analysis
Inhibition of tubulin polymerization in human HeLa cells assessed as fragmented or multipolar spindles formation at 20 to 50 nM by DAPI staining based immunofluorescence analysis
Inhibition of [3H]colchicine binding to tubulin (unknown origin) at 5 uM after 10 mins relative to control
Assay data:22 Tested
Inhibition of tubulin polymerization (unknown origin) preincubated for 15 mins followed by GTP addition measured for 20 mins by spectrophotometric method
Assay data:23 Active, 12 Activity ≤ 1 µM, 23 Tested
Inhibition of tubulin polymerization in human HeLa cells assessed as disrupted microtubule organization at 0.5 uM after 48 hrs by immunohistochemical analysis
Inhibition of tubulin polymerization (unknown origin)
Inhibition of tubulin polymerization (unknown origin) after 1 hr by fluorescence assay
Assay data:3 Active, 3 Tested
Inhibition of tubulin polymerization (unknown origin) at 3 uM after 1 hr by fluorescence assay relative to control
Assay data:3 Tested
Inhibition of tubulin (unknown origin) polymerization preincubated for 15 mins followed by GTP addition measured for 20 mins by turbidimetric analysis
Assay data:2 Active, 1 Activity ≤ 1 µM, 2 Tested
Inhibition of tubulin polymerization in human HeLa cells assessed as appearance of irregular microtubule at 3 uM after 24 hrs by DAPI staining based confocal microscopic analysis
Inhibition of tubulin polymerization in human HeLa cells assessed as increase of tubulin in soluble fraction at 3 uM after 24 hrs by Western blot analysis
Inhibition of tubulin polymerization in human HeLa cells assessed as disruption of microtubule organization at 3 uM after 24 hrs by DAPI staining based confocal microscopic analysis
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