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Inhibition of SPPL2a in mouse whole blood assessed as reduction in CD74/p8 NTF accumulation at 4 uM incubated for 5 hrs by flow cytometric analysis based assay
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Inhibition of mouse SPPL2a cotransfected with EGFP-NLP-fused TNFalpha (1 to 77 residues)-NTF incubated for 24 hrs by Hoechst staining based Cellomics ArrayScan analysis
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of SPPL2a in Balb/c mouse A20 cells assessed as reduction in CD74/p8 NTF accumulation incubated for overnight by Western blot analysis
Inhibition of mouse SPPL2a in A20 cells assessed as decrease of CD74/p8 accumulation at 2 uM preincubated for 16 hrs followed by compound wash measured after 2 hrs by Western blot method
Assay data:1 Tested
SummaryPubMed CitationRelated BioAssays by Target
Inhibition of mouse SPPL2a in A20 cells assessed as decrease of CD74/p8 accumulation at 2 uM preincubated for 16 hrs followed by compound wash measured after 50 mins by Western blot method
Inhibition of mouse SPPL2a expressed in human U2OS cells using EGFP-labeled TNFalpha (1 to 77 residues) NLS as substrate after 24 hrs by Hoechst staining based high content imaging assay
Inhibition of SPPL2a in mouse whole blood assessed as increase in CD74/p8 accumulation after 5.5 hrs by Western bot method
Assay data:2 Active, 2 Activity ≤ 1 µM, 2 Tested
Reversible inhibition of SPPL2a in mouse A20 cells assessed as increase in CD74/p8 accumulation at 2 uM preincubated for 16 hrs followed by compound wash out measured up to 2 hrs by Western blot method
Inhibition of mouse SPPL2a in A20 cells assessed as CD74/p8 accumulation by flow cytometry
Assay data:5 Active, 5 Activity ≤ 1 µM, 7 Tested
Genome-wide siRNA screen of genes regulating the Lipopolysaccharide-induced NF-kappaB and TNF-alpha responses in mouse macrophages_Primary screen NFkB readout
Assay data:762 Active, 16821 Tested
SummaryRelated BioAssays by Same Project
Genome-wide siRNA screen of genes regulating the Lipopolysaccharide-induced NF-kappaB and TNF-alpha responses in mouse macrophages_Primary screen TNF readout
Assay data:1106 Active, 16821 Tested
A screen to identify the genes that modify the response of osteosarcoma to doxorubicin in mouse - Primary Screen
Assay data:788 Active, 16789 Tested
SummaryPubMed Citation
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