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Specific activity at molybdenum cofactor/metal-free precursor molybopterin bound human recombinant mARC1 expressed in Escherichia coli TP1000 assessed as reduction to benzamidine in reconstituted system containing microsomal cyt b5 and NADH without microsomal NADH cytochrome b5 reductase per mg of protein at 0.5 mM by HPLC analysis
Assay data:1 Tested
SummaryPubMed CitationRelated BioAssays by Target
A whole genome RNAi screen highlights components of the ER/Golgi as a prominent source of resistance to immunotoxin-mediated cytotoxicity
Assay data:555 Active, 64752 Tested
SummaryPubMed Citation
RNAi screen for vorinostat resistance genes - Primary Screen
Assay data:450 Active, 18118 Tested
Genome-wide high-content siRNA screens for regulators of Parkin translocation in selective mitophagy (Ambion Library)
Assay data:9227 Active, 64752 Tested
Genome-wide high-content siRNA screens for regulators of Parkin translocation in selective mitophagy (Dharmacon Library)
Assay data:826 Active, 18119 Tested
An siRNA screen for human genes that are involved in human papilloma virus (HPV) E2 transcriptional repression of the E6 and E7 oncogenes
Assay data:497 Active, 21019 Tested
Activation of prodrug into pentamidine per mg of recombinant hmARC1 in 20 mM MES buffer of pH 6.0 for 30 mins
Activation of prodrug into benzamidine per mg of recombinant hmARC1 in 20 mM MES buffer of pH 6.0 for 30 mins
Specific activity at molybdenum cofactor/metal-free precursor molybopterin bound human recombinant mARC1 expressed in Escherichia coli TP1000 assessed as reduction to benzamidine in complete reconstituted system containing microsomal cyt b5, NADH and microsomal NADH cyt b5 reductase per mg of protein at 0.5 mM by HPLC analysis
Specific activity at molybdenum cofactor/metal-free precursor molybopterin bound human recombinant mARC1 expressed in Escherichia coli TP1000 assessed as reduction to benzamidine in reconstituted system containing NADH and microsomal NADH cyt b5 reductase without microsomal cytochrome b5 per mg of protein at 0.5 mM by HPLC analysis
InfectX: Pathogen infection siRNA screens
Assay data:115372 Tested
Specific activity at molybdenum cofactor/metal-free precursor molybopterin bound human recombinant mARC1 expressed in Escherichia coli TP1000 assessed as reduction to pentamidine in complete reconstituted system containing microsomal cyt b5, NADH and microsomal NADH cyt b5 reductase per mg of protein at 0.5 mM by HPLC analysis
Assay data:2 Tested
Specific activity at molybdenum cofactor/metal-free precursor molybopterin bound human recombinant mARC1 expressed in Escherichia coli TP1000 assessed as reduction to pentamidine monoamidoxime in complete reconstituted system containing microsomal cyt b5, NADH and microsomal NADH cyt b5 reductase per mg of protein at 0.5 mM by HPLC analysis
Specific activity at molybdenum cofactor/metal-free precursor molybopterin bound human recombinant mARC1 expressed in Escherichia coli TP1000 assessed as reduction to diminazene monoamidoxime in complete reconstituted system containing microsomal cyt b5, NADH and microsomal NADH cyt b5 reductase per mg of protein at 0.5 mM by HPLC analysis
Specific activity at molybdenum cofactor/metal-free precursor molybopterin bound human recombinant mARC1 expressed in Escherichia coli TP1000 assessed as reduction to diminazene in complete reconstituted system containing microsomal cyt b5, NADH and microsomal NADH cyt b5 reductase per mg of protein at 0.5 mM by HPLC analysis
Specific activity at molybdenum cofactor/metal-free precursor molybopterin bound human recombinant mARC1 expressed in Escherichia coli TP1000 assessed as reduction to melagatran in complete reconstituted system containing microsomal cyt b5, NADH and microsomal NADH cyt b5 reductase per mg of protein at 0.5 mM by HPLC analysis
Specific activity at molybdenum cofactor/metal-free precursor molybopterin bound human recombinant mARC1 expressed in Escherichia coli TP1000 assessed as reduction to guanabenz in complete reconstituted system containing microsomal cyt b5, NADH and microsomal NADH cyt b5 reductase per mg of protein at 0.5 mM by HPLC analysis
Specific activity at molybdenum cofactor/metal-free precursor molybopterin bound human recombinant mARC1 expressed in Escherichia coli TP1000 assessed as reduction to debrisoquine in complete reconstituted system containing microsomal cyt b5, NADH and microsomal NADH cyt b5 reductase per mg of protein at 0.5 mM by HPLC analysis
siRNA Circadian Assay
Assay data:527 Active, 33364 Tested
SummaryPubMed CitationRelated BioAssays by Depositor
Substrate activity at human recombinant mARC1 expressed in Escherichia coli assessed as enzyme-mediated N-(4-Aminobutyl)-N'-(2-methoxyethyl)guanidine formation at 3 mM and pH 6 preincubated for 3 mins followed by NADH addition measured after 20 mins under aerobic conditions by RP-HPLC analysis
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
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