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Induction of Smad1/5/8 phosphorylation in mouse MC3T3-E1 cells assessed as ratio of phosphorylated Smad1/5/8 to actin beta protein level at 30 uM measured after 30 mins by Western blot analysis relative to control
Assay data:2 Tested
SummaryPubMed CitationRelated BioAssays by Target
Induction of Smad1/5/8 phosphorylation in mouse MC3T3-E1 cells assessed as ratio of phosphorylated Smad1/5/8 to actin beta protein level at 30 uM measured after 15 mins by Western blot analysis relative to control
Induction of Smad1/5/8 phosphorylation in mouse MC3T3-E1 cells assessed as ratio of phosphorylated Smad1/5/8 to actin beta protein level at 30 uM measured after 5 mins by Western blot analysis relative to control
Activation of Smad1/5/8 in mouse C2C12 cells assessed as increase in BMP2-stimulated OPN mRNA expression at 10 uM preincubated with cells followed by BMP2 addition after 3 days by real-time PCR analysis
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Activation of Smad1/5/8 in mouse C2C12 cells assessed as increase in BMP2-stimulated OCL mRNA expression at 10 uM preincubated with cells followed by BMP2 addition after 3 days by real-time PCR analysis
Activation of Smad1/5/8 in mouse C2C12 cells assessed as increase in BMP2-stimulated Runx2 mRNA expression at 10 uM preincubated with cells followed by BMP2 addition after 3 days by real-time PCR analysis
Increase in BMP2-induced Smad1/5/8 nuclear translocation in mouse C2C12 cells assessed as ratio of Smad1/5/8 to actin in cytosol fraction at 10 uM preincubated with cells followed by BMP2 addition for 4 hrs by Western blot analysis (Rvb = 0.4 No_unit)
Assay data:1 Tested
Increase in BMP2-induced Smad1/5/8 nuclear translocation in mouse C2C12 cells assessed as ratio of Smad1/5/8 to lamin B1 in nuclear fraction at 10 uM preincubated with cells followed by BMP2 addition for 4 hrs by Western blot analysis (Rvb = 1.5 No_unit)
Increase in BMP2-induced Smad1/5/8 phosphorylation in mouse C2C12 cells assessed as ratio of phosphorylated Smad1/5/8 to Smad1/5/8 in nuclear fraction at 10 uM preincubated with cells followed by BMP2 addition for 4 hrs by Western blot analysis (Rvb = 1.2 No_unit)
Genome-wide siRNA screen of genes regulating the Lipopolysaccharide-induced NF-kappaB and TNF-alpha responses in mouse macrophages_Primary screen NFkB readout
Assay data:762 Active, 16821 Tested
SummaryRelated BioAssays by Same Project
Genome-wide siRNA screen of genes regulating the Lipopolysaccharide-induced NF-kappaB and TNF-alpha responses in mouse macrophages_Primary screen TNF readout
Assay data:1106 Active, 16821 Tested
A screen to identify the genes that modify the response of osteosarcoma to doxorubicin in mouse - Primary Screen
Assay data:788 Active, 16789 Tested
SummaryPubMed Citation
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