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SidA Activity Assay from Article 10.1021/acschembio.6b00666: "Inhibition of the Flavin-Dependent Monooxygenase Siderophore A (SidA) Blocks Siderophore Biosynthesis and Aspergillus fumigatus Growth."
Assay data:1 Tested
SummaryPubMed CitationRelated BioAssays by DepositorRelated BioAssays by Target
ADP-TAMRA Binding Assay from Article 10.1021/acschembio.6b00666: "Inhibition of the Flavin-Dependent Monooxygenase Siderophore A (SidA) Blocks Siderophore Biosynthesis and Aspergillus fumigatus Growth."
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by DepositorRelated BioAssays by Target
Enzyme Inhibition Assay from US Patent US9156811: "N-myristoyl transferase inhibitors"
Assay data:387 Active, 10 Activity ≤ 1 nM, 289 Activity ≤ 1 µM, 505 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMRelated BioAssays by Target
Non-competitive inhibition of Aspergillus fumigatus N-terminal 6His-tagged SidA expressed in Escherichia coli BL21 (DE3) using L-ornithine as substrate after 20 mins by UPLC analysis
SummaryPubMed CitationRelated BioAssays by Target
Non-competitive inhibition of Aspergillus fumigatus N-terminal 6His-tagged SidA expressed in Escherichia coli BL21 (DE3) by FP assay
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Inhibition of recombinant Aspergillus fumigatus NMT (Gln86 to Leu492 residues) expressed in Escherichia coli BL21(DE3) pLysS cells using [3H]-myristoyl coenzyme A/CAP5.5 as substrate after 20 mins by scintillation proximity assay
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Binding affinity to Aspergillus fumigatus AF293 sterol 14-alpha demethylase isoenzyme A expressed in Escherichia coli
Assay data:4 Active, 5 Tested
Substrate activity at recombinant N-terminal His6-fusion tagged Aspergillus fumigatus FgaPT2 expressed in Escherichia coli BL21 (DE3) assessed as alkylation of L-tryptophan by measuring product formation at pH 7.5 in Tris buffer after 16 hrs by RP-HPLC analysis
Assay data:9 Tested
Substrate activity at recombinant N-terminal His6-fusion tagged Aspergillus fumigatus FgaPT2 expressed in Escherichia coli BL21 (DE3) assessed as ratio of Kcat/Km in presence of L-tryptophan by Michaelis-Menten plot analysis
Assay data:19 Tested
Substrate activity at recombinant N-terminal His6-fusion tagged Aspergillus fumigatus FgaPT2 expressed in Escherichia coli BL21 (DE3) assessed as Km in presence of L-tryptophan by Michaelis-Menten plot analysis
Substrate activity at recombinant N-terminal His6-fusion tagged Aspergillus fumigatus FgaPT2 expressed in Escherichia coli BL21 (DE3) assessed as Kcat in presence of L-tryptophan by Michaelis-Menten plot analysis
Substrate activity at recombinant N-terminal His6-fusion tagged Aspergillus fumigatus FgaPT2 expressed in Escherichia coli BL21 (DE3) assessed as alkylation of L-tryptophan by measuring product formation at pH 7.5 in Tris buffer after 16 hrs by RP-HPLC analysis relative to control
Assay data:25 Tested
Ratio Kcat to Km for Aspergillus fumigatus FtmPT1 by HPLC analysis relative to L-tryptophan
Activity at 1.62 uM Aspergillus fumigatus FtmPT1 assessed as compound conversion at 1 mM after 2 hrs by HPLC analysis in presence of dimethylallyl diphosphate
Activity at 0.54 uM Aspergillus fumigatus FtmPT1 assessed as compound conversion at 1 mM after 2 hrs by HPLC analysis in presence of dimethylallyl diphosphate
Activity at 0.28 uM Aspergillus fumigatus FtmPT1 assessed as compound conversion at 1 mM after 2 hrs by HPLC analysis in presence of dimethylallyl diphosphate
Activity at 1.12 uM Aspergillus fumigatus FtmPT1 assessed as compound conversion rate at 1 mM after 16 hrs by HPLC analysis in presence of dimethylallyl diphosphate
Activity at 1.12 uM Aspergillus fumigatus FtmPT1 assessed as compound retention time after 2 hrs by HPLC analysis
Activity at Aspergillus fumigatus FtmPT1 by HPLC analysis
Assay data:2 Tested
Activity at 1.68 uM Aspergillus fumigatus FtmPT1 assessed as compound conversion rate at 1 mM after 16 hrs by HPLC analysis in presence of dimethylallyl diphosphate relative to L-tryptophan
Assay data:15 Tested
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