LOCUS NM_013170 3775 bp mRNA linear ROD 03-APR-2024
DEFINITION Rattus norvegicus guanylate cyclase 2C (Gucy2c), mRNA.
ACCESSION NM_013170 XM_346727
VERSION NM_013170.1
KEYWORDS RefSeq; RefSeq Select.
SOURCE Rattus norvegicus (Norway rat)
ORGANISM Rattus norvegicus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Rattus.
REFERENCE 1 (bases 1 to 3775)
AUTHORS Akieda-Asai,S., Ida,T., Miyazato,M., Kangawa,K. and Date,Y.
TITLE Interleukin-15 derived from Guanylin-GC-C-expressing macrophages
inhibits fatty acid synthase in adipocytes
JOURNAL Peptides 99, 14-19 (2018)
PUBMED 29097254
REMARK GeneRIF: These results support the hypothesis that IL-15 secreted
from Gn-GC-C-expressing macrophages contributes to the inhibition
of fatty acid synthase and lipid accumulation in adipocytes,
leading to obesity resistance
REFERENCE 2 (bases 1 to 3775)
AUTHORS Hasegawa,K., Akieda-Asai,S., Fujii,Y., Bae,C.R., Yasuda,M. and
Date,Y.
TITLE Guanylin-Guanylyl cyclase-C signaling in macrophages regulates
mesenteric fat inflammation induced by high-fat diet
JOURNAL Endocr J 62 (10), 939-947 (2015)
PUBMED 26249840
REMARK GeneRIF: Data (including data from studies in transgenic rats)
suggest guanylin--guanylyl cyclase-C signaling in macrophages in
mesenteric fat regulates cGMP--PKG--VASP pathway and mesenteric
panniculitis/obesity via down-regulation of proinflammatory
factors.
REFERENCE 3 (bases 1 to 3775)
AUTHORS Akieda-Asai,S., Sugiyama,M., Miyazawa,T., Koda,S., Okano,I.,
Senba,K., Poleni,P.E., Hizukuri,Y., Okamoto,A., Yamahara,K.,
Mutoh,E., Aoyama,F., Sawaguchi,A., Furuya,M., Miyazato,M.,
Kangawa,K. and Date,Y.
TITLE Involvement of guanylin and GC-C in rat mesenteric macrophages in
resistance to a high-fat diet
JOURNAL J Lipid Res 54 (1), 85-96 (2013)
PUBMED 23081987
REMARK GeneRIF: the macrophagic guanylin-GC-C system contributes to the
altered expression of genes involved in lipid metabolism, leading
to resistance to obesity
REFERENCE 4 (bases 1 to 3775)
AUTHORS Qian,X., Moss,N.G., Fellner,R.C., Taylor-Blake,B. and Goy,M.F.
TITLE The rat kidney contains high levels of prouroguanylin (the
uroguanylin precursor) but does not express GC-C (the enteric
uroguanylin receptor)
JOURNAL Am J Physiol Renal Physiol 300 (2), F561-F573 (2011)
PUBMED 21106860
REMARK GeneRIF: these studies provide a comprehensive description of Ugn
and GC-C expression in the kidney of the Sprague-Dawley rat.
REFERENCE 5 (bases 1 to 3775)
AUTHORS Saha,S., Biswas,K.H., Kondapalli,C., Isloor,N. and
Visweswariah,S.S.
TITLE The linker region in receptor guanylyl cyclases is a key regulatory
module: mutational analysis of guanylyl cyclase C
JOURNAL J Biol Chem 284 (40), 27135-27145 (2009)
PUBMED 19648115
REMARK GeneRIF: the role of the linker region in receptor guanylyl
cyclases
REFERENCE 6 (bases 1 to 3775)
AUTHORS Li,P., Schulz,S., Bombonati,A., Palazzo,J.P., Hyslop,T.M., Xu,Y.,
Baran,A.A., Siracusa,L.D., Pitari,G.M. and Waldman,S.A.
TITLE Guanylyl cyclase C suppresses intestinal tumorigenesis by
restricting proliferation and maintaining genomic integrity
JOURNAL Gastroenterology 133 (2), 599-607 (2007)
PUBMED 17681179
REFERENCE 7 (bases 1 to 3775)
AUTHORS Kwitek,A.E., Gullings-Handley,J., Yu,J., Carlos,D.C., Orlebeke,K.,
Nie,J., Eckert,J., Lemke,A., Andrae,J.W., Bromberg,S., Pasko,D.,
Chen,D., Scheetz,T.E., Casavant,T.L., Soares,M.B., Sheffield,V.C.,
Tonellato,P.J. and Jacob,H.J.
TITLE High-density rat radiation hybrid maps containing over 24,000
SSLPs, genes, and ESTs provide a direct link to the rat genome
sequence
JOURNAL Genome Res 14 (4), 750-757 (2004)
PUBMED 15060019
REFERENCE 8 (bases 1 to 3775)
AUTHORS Lorenz,J.N., Nieman,M., Sabo,J., Sanford,L.P., Hawkins,J.A.,
Elitsur,N., Gawenis,L.R., Clarke,L.L. and Cohen,M.B.
TITLE Uroguanylin knockout mice have increased blood pressure and
impaired natriuretic response to enteral NaCl load
JOURNAL J Clin Invest 112 (8), 1244-1254 (2003)
PUBMED 14561709
REFERENCE 9 (bases 1 to 3775)
AUTHORS Schulz,S., Lopez,M.J., Kuhn,M. and Garbers,D.L.
TITLE Disruption of the guanylyl cyclase-C gene leads to a paradoxical
phenotype of viable but heat-stable enterotoxin-resistant mice
JOURNAL J Clin Invest 100 (6), 1590-1595 (1997)
PUBMED 9294128
REFERENCE 10 (bases 1 to 3775)
AUTHORS Schulz,S., Green,C.K., Yuen,P.S. and Garbers,D.L.
TITLE Guanylyl cyclase is a heat-stable enterotoxin receptor
JOURNAL Cell 63 (5), 941-948 (1990)
PUBMED 1701694
COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final
NCBI review. The reference sequence was derived from M55636.1.
On Dec 17, 2003 this sequence version replaced XM_346727.1.
##Evidence-Data-START##
Transcript exon combination :: M55636.1 [ECO:0000332]
RNAseq introns :: mixed sample support SAMEA5756307,
SAMEA5760383 [ECO:0006172]
##Evidence-Data-END##
##RefSeq-Attributes-START##
RefSeq Select criteria :: based on conservation, expression,
longest protein
##RefSeq-Attributes-END##
FEATURES Location/Qualifiers
source 1..3775
/organism="Rattus norvegicus"
/mol_type="mRNA"
/strain="Sprague-Dawley"
/db_xref="taxon:10116"
/chromosome="4"
/map="4q43"
gene 1..3775
/gene="Gucy2c"
/gene_synonym="GCC"
/note="guanylate cyclase 2C"
/db_xref="GeneID:25711"
/db_xref="RGD:2771"
exon 1..224
/gene="Gucy2c"
/gene_synonym="GCC"
/inference="alignment:Splign:2.1.0"
CDS 11..3229
/gene="Gucy2c"
/gene_synonym="GCC"
/EC_number="4.6.1.2"
/note="heat-stable enterotoxin receptor; GC-C; STA
receptor; intestinal guanylate cyclase; guanylyl cyclase
C; Guanylate cyclase 2C (heat stable enterotoxin
receptor)"
/codon_start=1
/product="guanylyl cyclase C precursor"
/protein_id="NP_037302.1"
/db_xref="GeneID:25711"
/db_xref="RGD:2771"
/translation="MTSLLGLAVRLLLFQPTLMFWASQVRQKCHNGTYEISVLMMDNS
AYKEPLQNLRDAVEEGLDIVRKRLREAELNVTVNATFIYSDGLIHKSGDCRSSTCEGL
DLLREITRDRKMGCVLMGPSCTYSTFQMYLDTELNYPMISAGSFGLSCDYKETLTRIL
PPARKLMYFLVDFWKVNNAPFKTFSWNSSYVYKNGSEPEDCFWYLNALEAGVSYFSEV
LSFKDVLRRSEQFQEILMGRNRKSNVIVMCGTPETFYNVKGDLKVADDTVVILVDLFS
NHYFEDDTRAPEYMDNVLVLTLPPEKFIANASVSGRFPSERSDFSLAYLEGTLLFGHM
LQTFLENGESVTTPKFARAFRNLTFQGLEGPVTLDDSGDIDNIMCLLYVSLDTRKYKV
LMAYDTHKNQTIPVATSPNFIWKNHRLPNDVPGLGPQILMIAVFTLTGIVVVLLLIAL
LVLRKYRRDHELRQKKWSHIPSENIFPLETNETNHVSLKIDDDRRRDTIQRVRQCKYD
KKKVILKDLKHCDGNFSEKQKIELNKLLQSDYYNLTKFYGTVKLDTRIFGVVEYCERG
SLREVLNDTISYPDGTFMDWEFKISVLNDIAKGMSYLHSSKIEVHGRLKSTNCVVDSR
MVVKITDFGCNSILPPKKDLWTAPEHLRQATISQKGDVYSFSIIAQEIILRKETFYTL
SCRDQNEKIFRVENSYGTKPFRPDLFLETADEKELEVYLLVKSCWEEDPEKRPDFKKI
ESTLAKIFGLFHDQKNESYMDTLIRRLQLYSRNLEHLVEERTQLYKAERDRADHLNFM
LLPRLVVKSLKEKGIVEPELYEEVTIYFSDIVGFTTICKYSTPMEVVDMLNDIYKSFD
QIVDHHDVYKVETIGDAYVVASGLPMRNGNRHAVDISKMALDILSFMGTFELEHLPGL
PVWIRIGVHSGPCAAGVVGIKMPRYCLFGDTVNTASRMESTGLPLRIHMSSSTIAILR
RTDCQFLYEVRGETYLKGRGTETTYWLTGMKDQEYNLPTPPTVENQQRLQTEFSDMIV
SALQKRQASGVKSRRPTRVASYKKGFLEYMQLNNSDHDSTYF"
sig_peptide 11..76
/gene="Gucy2c"
/gene_synonym="GCC"
mat_peptide 77..3226
/gene="Gucy2c"
/gene_synonym="GCC"
/product="guanylyl cyclase C"
misc_feature 101..103
/gene="Gucy2c"
/gene_synonym="GCC"
/note="N-linked (GlcNAc...) asparagine.
/evidence=ECO:0000255; propagated from
UniProtKB/Swiss-Prot (P23897.2); glycosylation site"
misc_feature 230..232
/gene="Gucy2c"
/gene_synonym="GCC"
/note="N-linked (GlcNAc...) asparagine.
/evidence=ECO:0000255; propagated from
UniProtKB/Swiss-Prot (P23897.2); glycosylation site"
misc_feature 242..244
/gene="Gucy2c"
/gene_synonym="GCC"
/note="N-linked (GlcNAc...) asparagine.
/evidence=ECO:0000255; propagated from
UniProtKB/Swiss-Prot (P23897.2); glycosylation site"
misc_feature 569..571
/gene="Gucy2c"
/gene_synonym="GCC"
/note="N-linked (GlcNAc...) asparagine.
/evidence=ECO:0000255; propagated from
UniProtKB/Swiss-Prot (P23897.2); glycosylation site"
misc_feature 590..592
/gene="Gucy2c"
/gene_synonym="GCC"
/note="N-linked (GlcNAc...) asparagine.
/evidence=ECO:0000255; propagated from
UniProtKB/Swiss-Prot (P23897.2); glycosylation site"
misc_feature 926..928
/gene="Gucy2c"
/gene_synonym="GCC"
/note="N-linked (GlcNAc...) asparagine.
/evidence=ECO:0000255; propagated from
UniProtKB/Swiss-Prot (P23897.2); glycosylation site"
misc_feature 1211..1213
/gene="Gucy2c"
/gene_synonym="GCC"
/note="N-linked (GlcNAc...) asparagine.
/evidence=ECO:0000255; propagated from
UniProtKB/Swiss-Prot (P23897.2); glycosylation site"
misc_feature 1298..1369
/gene="Gucy2c"
/gene_synonym="GCC"
/note="propagated from UniProtKB/Swiss-Prot (P23897.2);
transmembrane region"
exon 225..337
/gene="Gucy2c"
/gene_synonym="GCC"
/inference="alignment:Splign:2.1.0"
exon 338..402
/gene="Gucy2c"
/gene_synonym="GCC"
/inference="alignment:Splign:2.1.0"
exon 403..618
/gene="Gucy2c"
/gene_synonym="GCC"
/inference="alignment:Splign:2.1.0"
exon 619..740
/gene="Gucy2c"
/gene_synonym="GCC"
/inference="alignment:Splign:2.1.0"
exon 741..837
/gene="Gucy2c"
/gene_synonym="GCC"
/inference="alignment:Splign:2.1.0"
exon 838..955
/gene="Gucy2c"
/gene_synonym="GCC"
/inference="alignment:Splign:2.1.0"
exon 956..1091
/gene="Gucy2c"
/gene_synonym="GCC"
/inference="alignment:Splign:2.1.0"
exon 1092..1177
/gene="Gucy2c"
/gene_synonym="GCC"
/inference="alignment:Splign:2.1.0"
exon 1178..1289
/gene="Gucy2c"
/gene_synonym="GCC"
/inference="alignment:Splign:2.1.0"
exon 1290..1371
/gene="Gucy2c"
/gene_synonym="GCC"
/inference="alignment:Splign:2.1.0"
exon 1372..1477
/gene="Gucy2c"
/gene_synonym="GCC"
/inference="alignment:Splign:2.1.0"
exon 1478..1540
/gene="Gucy2c"
/gene_synonym="GCC"
/inference="alignment:Splign:2.1.0"
exon 1541..1612
/gene="Gucy2c"
/gene_synonym="GCC"
/inference="alignment:Splign:2.1.0"
exon 1613..1717
/gene="Gucy2c"
/gene_synonym="GCC"
/inference="alignment:Splign:2.1.0"
exon 1718..1804
/gene="Gucy2c"
/gene_synonym="GCC"
/inference="alignment:Splign:2.1.0"
exon 1805..1937
/gene="Gucy2c"
/gene_synonym="GCC"
/inference="alignment:Splign:2.1.0"
exon 1938..2075
/gene="Gucy2c"
/gene_synonym="GCC"
/inference="alignment:Splign:2.1.0"
exon 2076..2164
/gene="Gucy2c"
/gene_synonym="GCC"
/inference="alignment:Splign:2.1.0"
exon 2165..2256
/gene="Gucy2c"
/gene_synonym="GCC"
/inference="alignment:Splign:2.1.0"
exon 2257..2415
/gene="Gucy2c"
/gene_synonym="GCC"
/inference="alignment:Splign:2.1.0"
exon 2416..2608
/gene="Gucy2c"
/gene_synonym="GCC"
/inference="alignment:Splign:2.1.0"
exon 2609..2783
/gene="Gucy2c"
/gene_synonym="GCC"
/inference="alignment:Splign:2.1.0"
exon 2784..2882
/gene="Gucy2c"
/gene_synonym="GCC"
/inference="alignment:Splign:2.1.0"
exon 2883..2977
/gene="Gucy2c"
/gene_synonym="GCC"
/inference="alignment:Splign:2.1.0"
exon 2978..3054
/gene="Gucy2c"
/gene_synonym="GCC"
/inference="alignment:Splign:2.1.0"
exon 3055..3775
/gene="Gucy2c"
/gene_synonym="GCC"
/inference="alignment:Splign:2.1.0"
ORIGIN
1 gagcgaggtc atgacgtcac tcctgggctt ggctgtgcgg ttactgctct tccaacccac
61 gctgatgttc tgggcctccc aggtgaggca gaagtgccac aatggcacct acgagatcag
121 tgtcctgatg atggataact cagcctacaa agaacctttg caaaacttga gggatgctgt
181 ggaggaagga ctggacatcg tgcgaaagcg cctgcgcgaa gccgaactaa atgtgactgt
241 gaacgcaacc ttcatctact ccgatggtct gatccataag tcaggtgact gccggagcag
301 tacctgtgaa ggccttgacc tcctcaggga aattacaaga gatcgtaaga tgggctgtgt
361 cctcatgggg ccctcgtgca cgtattccac cttccagatg taccttgaca cagagttgaa
421 ctatcccatg atttccgctg gaagttttgg attgtcctgt gactataagg aaaccctaac
481 caggatcctg cctccagcca ggaagctgat gtacttcttg gtcgatttct ggaaagtcaa
541 caatgcacct ttcaaaacct tttcctggaa ctcttcatat gtttacaaga acggatcgga
601 gcctgaagat tgtttctggt acctcaacgc tctagaggct ggggtgtcct atttttctga
661 ggtgctcagc ttcaaggatg tattgagacg cagtgaacag ttccaggaaa tcctaatggg
721 ccgtaacagg aagagcaatg tgattgttat gtgtggcacg ccagaaacct tctacaatgt
781 gaaaggtgac ctcaaagtgg ctgacgacac tgttgtcatc ctggtagatc tgttcagtaa
841 ccattacttt gaggatgaca ccagagctcc tgagtatatg gacaatgtcc tcgtcctgac
901 actgcctcct gaaaagttca tcgcgaacgc ctctgtctct gggaggtttc catcggaaag
961 aagcgacttt tctctcgctt acttggaggg gaccttgctg tttggacaca tgctgcagac
1021 gtttcttgaa aatggagaat ctgtcaccac gcccaagttc gctcgtgcgt tcaggaatct
1081 cacttttcaa ggcttagagg ggcccgtgac tctggatgac agtggggaca ttgacaacat
1141 tatgtgtctt ctgtatgtgt ctctggatac caggaaatac aaggttctta tggcgtatga
1201 cacccataaa aaccaaacga tccctgtggc tacgagcccc aacttcatct ggaagaacca
1261 cagactccct aatgacgttc ctgggctggg ccctcaaatc ctgatgattg ccgtcttcac
1321 gctcacgggg attgtggtcg ttctgctgct gattgccctc cttgtgctca gaaaatacag
1381 aagagatcat gaacttcgac agaagaaatg gtcccacatc ccttctgaaa atatctttcc
1441 tctggagacc aacgagacca accatgtcag cctgaagatt gacgatgaca ggaggcggga
1501 tacaatccag agagtgcgac agtgcaaata cgacaagaag aaagtgatcc tgaaagacct
1561 caagcactgt gatggtaact tcagtgagaa gcagaagata gaactgaaca agctactgca
1621 gtcagactac tacaacctga ccaagttcta cggcaccgtg aagctagaca ccaggatctt
1681 tggggtggtc gagtactgcg agagggggtc cctccgggaa gtgttaaatg acacgatttc
1741 ctaccctgat ggcacgttca tggattggga gtttaagatc tctgtcttaa atgacattgc
1801 taaggggatg tcctatctgc actccagtaa gattgaagtc cacgggcgtc tgaagtccac
1861 caactgcgtg gtggacagtc gcatggtggt gaagatcact gattttgggt gcaattccat
1921 cctgcctcca aagaaagacc tgtggactgc ccccgagcac ctgcgccaag ctactatctc
1981 tcagaaagga gacgtgtaca gcttcagcat cattgcccag gagatcatcc tccgcaagga
2041 aactttctac acgctgagct gccgggatca gaatgagaag attttcagag tggaaaattc
2101 ctatgggacg aaacccttcc gcccagatct cttcctggaa accgcagatg agaaggagct
2161 ggaggtctat ctattggtca aaagctgttg ggaggaggat ccagaaaaga gaccagattt
2221 caagaaaatc gagagcacac tagccaagat atttggcctt tttcatgacc aaaaaaatga
2281 atcttacatg gacaccttga tccgacgtct acagctgtat tctcggaacc tggagcacct
2341 ggtggaggaa aggactcagc tgtacaaggc cgagagggac agggctgacc accttaactt
2401 tatgctgctc ccacggctgg tggtaaagtc cctgaaggag aaaggcatcg tggagccaga
2461 gctgtacgaa gaagtcacaa tctatttcag tgacattgtc ggtttcacga ccatctgcaa
2521 gtacagcacg cccatggagg tggtggacat gctgaatgac atctacaaga gttttgacca
2581 gattgtggat caccacgacg tctacaaggt agaaaccatc ggcgatgcct acgtggtggc
2641 cagcggcctg cctatgagaa acggcaaccg gcatgcagtg gacatttcca agatggcctt
2701 ggacatcctc agcttcatgg ggacctttga gctggagcat ctccccggcc tccccgtgtg
2761 gattcgcatt ggggttcatt ctggcccctg tgctgctggt gtggtgggga tcaagatgcc
2821 tcgttattgc ctgtttggag acactgtcaa cactgcctcc aggatggagt ccaccggcct
2881 tcccttaagg attcacatga gcagctccac cattgccatc ctgaggagaa cggattgcca
2941 gttcctgtac gaagtgaggg gagaaacgta cttaaaggga agagggaccg agaccacata
3001 ctggctgact gggatgaagg accaagagta caacctgcca accccaccaa cagtggagaa
3061 ccaacagcgt ctgcaaactg agttctcaga catgatcgtt agtgccttac agaaaagaca
3121 ggcctcgggc gtgaagagcc ggaggcccac tcgggtggcc agctacaaga aaggcttttt
3181 ggaatacatg cagctgaaca actcagacca cgatagcacc tatttttaga ccacgtgcgg
3241 tctaagaact gacagtagca acctctgata tcctgaatct gcattttccc agaaacctca
3301 acaacacaga caagtgctta gccccagtgc cctgtctgga atgtagaacc agcccccaag
3361 tcatgtgggt gttctgggtt gggttgggtt gggtttggtt ggttggtttt gtttctattg
3421 agacagagtc tcatgtatcc caaactggcc tcaaactcgc tgaggagcta tggatgacct
3481 tggacttcta agaccatcca tgtgtgttcc tggctgtgtg atgccctgtc cagagtcgtg
3541 tcccacagtt ctccacggag catcaacgtc agcctgaagg gaggaaggag gaacgtacta
3601 tacagaactt ggggtttcat tctaatttca tttctgcttt ttttcatttt gtttactgga
3661 tccttcctta tgtacacatg aattttttta attgtctgga ttaagtagct tatctccaag
3721 aaagtgtgtt taactagtga tttttgcaga aaccatgctg gatattaggt aaaaa
//