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Bacillus pseudomycoides strain AFS095135 AFS095135_100_B5_Contig81_140514, whole genome shotgun sequence

NCBI Reference Sequence: NZ_NVOF01000081.1

FASTA Graphics 

LOCUS       NZ_NVOF01000081         7277 bp    DNA     linear   CON 23-AUG-2024
DEFINITION  Bacillus pseudomycoides strain AFS095135
            AFS095135_100_B5_Contig81_140514, whole genome shotgun sequence.
ACCESSION   NZ_NVOF01000081 NZ_NVOF01000000
VERSION     NZ_NVOF01000081.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07598649
            Assembly: GCF_002553115.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus pseudomycoides
  ORGANISM  Bacillus pseudomycoides
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales; Bacillaceae;
            Bacillus; Bacillus cereus group.
REFERENCE   1  (bases 1 to 7277)
  AUTHORS   Bleich,R.M., Grubbs,K.J., Santa Maria,K.C., Allen,S.E., Farag,S.,
            Shank,E.A. and Bowers,A.
  CONSRTM   Agbiome Team Llc
  TITLE     Large-scale bioinformatics analysis of Bacillus genomes uncovers
            conserved roles of natural products in bacterial physiology
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7277)
  AUTHORS   Bleich,R.M., Grubbs,K.J., Santa Maria,K.C., Allen,S.E., Farag,S.,
            Shank,E.A. and Bowers,A.
  CONSRTM   Agbiome Team Llc
  TITLE     Direct Submission
  JOURNAL   Submitted (01-SEP-2017) Chemical Biology and Medicinal Chemistry,
            UNC Chapel Hill, 125 Mason Farm Rd., Chapel Hill, NC 17599, USA
COMMENT     REFSEQ INFORMATION: The reference sequence is identical to
            NVOF01000081.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: CLC Assembly Cell v. 4.2.72
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 63.03x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002553115.1-RS_2024_08_23
            Annotation Date                   :: 08/23/2024 08:51:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,970
            CDSs (total)                      :: 5,882
            Genes (coding)                    :: 5,456
            CDSs (with protein)               :: 5,456
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 426
            CDSs (without protein)            :: 426
            Pseudo Genes (ambiguous residues) :: 0 of 426
            Pseudo Genes (frameshifted)       :: 117 of 426
            Pseudo Genes (incomplete)         :: 339 of 426
            Pseudo Genes (internal stop)      :: 89 of 426
            Pseudo Genes (multiple problems)  :: 105 of 426
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7277
                     /organism="Bacillus pseudomycoides"
                     /mol_type="genomic DNA"
                     /submitter_seqid="AFS095135_100_B5_Contig81_140514"
                     /strain="AFS095135"
                     /isolation_source="plant leaf"
                     /db_xref="taxon:64104"
                     /geo_loc_name="USA: IN"
                     /collection_date="2014-01-14"
                     /collected_by="AgBiome"
     gene            complement(<1..896)
                     /locus_tag="CON69_RS25970"
                     /old_locus_tag="CON69_25965"
     CDS             complement(<1..896)
                     /locus_tag="CON69_RS25970"
                     /old_locus_tag="CON69_25965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016107982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="WP_098716094.1"
                     /translation="MKKAILPVLMSAMFVVPAVSHAENQEAVKQLTPKTEQAMQKTGW
                     VKEGNTWYFYDQNSVKKTGWLKDNGTWYYLNADGAMKSNGWLSDAGKWYYFAESGAMK
                     TGNIFNQGSCYLLGADGALYTNEGWVQYEGEWYYVHQGGNLHEGYLEYNGKIYYMNPG
                     SGDMKVNNWIGSHPDGNYFLSDGAMATGWNLINNEWYYFEESRTHFNTHNGWLSYKGS
                     WYYFVASKMKTGWIEDNGNWYYLNADGTMKTGWLKDKGTWYYLNANGTMKSSANGWDE
                     GWLDEGGKRYVFADSGAMWTGK"
     gene            complement(1289..1963)
                     /locus_tag="CON69_RS25975"
                     /old_locus_tag="CON69_25970"
     CDS             complement(1289..1963)
                     /locus_tag="CON69_RS25975"
                     /old_locus_tag="CON69_25970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003209947.1"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="WP_003209947.1"
                     /translation="MKTIKKIRIMLVDDQPIIRQGLTYIFNSQSDMEVVGEADNGKDA
                     VKIVLRCIPDIVLMDVQMPQATGIDATRNIVQSLPDTKIILLTTFDVQDYVFDGIRAG
                     AAGYLLKDTQTKELLDGVRSIQRGATLYNSMTAKQALAKIINSRDNISTNQPKASSFI
                     EPLTDREIEILQLIAYGKKNKEIANILYISEGIVKTHVHNINQKLEVTDRTQAVVLGI
                     CMQLVR"
     gene            complement(1982..2266)
                     /locus_tag="CON69_RS25980"
                     /old_locus_tag="CON69_25975"
     CDS             complement(1982..2266)
                     /locus_tag="CON69_RS25980"
                     /old_locus_tag="CON69_25975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018768121.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0000155 - phosphorelay sensor kinase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="WP_003204416.1"
                     /translation="MFRILQESITNVIRHSGANEVDVCIQEHAQELSLIVSDNGQFLS
                     GNLYQNGFGVKGMIERCQSVGGTCNFSVNSDGGLKVEAIIPLMNRVCDEA"
     gene            2722..4962
                     /locus_tag="CON69_RS25985"
                     /old_locus_tag="CON69_25980"
     CDS             2722..4962
                     /locus_tag="CON69_RS25985"
                     /old_locus_tag="CON69_25980"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002189720.1"
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_098716093.1"
                     /translation="MLKIIKHLKPFVASIIAVICLLSIQAVCDLSLPDYMSNIVNVGI
                     QQKGVENAVPGVIRKSELDKLKLFMNENEKKKVDDNYILLDKNNLSQSELKNDLKDYP
                     QLDKEPLYKLHTEDKNTINELNDIFGKPMLITQGIEKGGSSAFTAPGDNKAPAKLPPN
                     TDPFAVIAKLPQDQIDAMKEKADEKFKNMPGSMVTQSAVSFIENEYKKIGINTDKLQS
                     NYILTSGGKMLLLSLVSMAATVIVSLLAAKVAAGLGRDLRKKVFRKVTSFSNAEFDKF
                     STASLITRSTNDIQQVQTLMVMLLRIVFYAPILGIGGIIKVLTTDLSMGWIIAVAVIA
                     ILSLVIGLFSIAIPKFKSIQKLVDKINLITRESLTGMLVIRAFNNQKHEEKKFEKGNQ
                     DLTRTNLFVSRLMSFMMPMMMLIMNAVTVLIIWVGSHQVDMGHMQVGDMMAFMQYTMQ
                     IIMAFLMISMVSIMVPRASVSAQRIAEVLDTDLSIHDAAEPKTFASDKKGYVEFKNVS
                     FKYPGAEEDVLSNITFTAKPGETTAFIGSTGSGKSTLINLIPRFYDVTSGQILIDGTD
                     IREVSQKELREKIGYVPQKGVLFSGTIESNLKYGKKEATEEELAKAAEIAQAMEFINA
                     KSESFRTEISQGGTNVSGGQKQRLSIARALTKKSEIFIFDDSFSALDFKTDAALRRAL
                     NNEIAGSTILLVAQRISTIMNADKIIVLDKGKIVGTGTHEELMENCEVYKQIALSQLS
                     REELSS"
     gene            4959..6824
                     /locus_tag="CON69_RS25990"
                     /old_locus_tag="CON69_25985"
     CDS             4959..6824
                     /locus_tag="CON69_RS25990"
                     /old_locus_tag="CON69_25985"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002165711.1"
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_098623328.1"
                     /translation="MSDRKIEHNRQGGHGGGHMGGGMRKIEKAKNFKGTMNKLLQYLK
                     PYKFSIVIVIIFAIGSAAFAIVGPKILGKATTKLFEGLVDKVTGVHGAAIDFTYIGNI
                     ALLLLGLYIASTVFSIIQGYIISGVAQKVSYNFRKEIDEKINRMPLKYFDKTTHGEVL
                     SRVTNDVDTVSQTLNQSMSQIITSVITIIGVLIMMLSISWQMTLVALLILPVSMTLIM
                     GVVKRSQKYFKSQQEYLGHVNGQVEEIYSGHNIVKAFNTEEEEVKKFEEVNDTLYHSA
                     WKSQFLSGMMMPIMMFIGNIGYVAVSILGGWLAVKRTIAVGDILAFVQYVRSFTQPIA
                     QVAQIANVLQSTAAAAERVFEFLDEEEEIPEAENPVKLQEVKGEVTFKDVQFGYNPDK
                     IIINNFSANIKPGQKVAIVGPTGAGKTTIVKLLMRFYDVNSGAICIDGHDIKDFTRED
                     LRSMFGMVLQDTWLFNGSIMENIRYGRLGATDEEVIEAAKAAHVHSFVKTLPNKYQME
                     LNEEASNVSQGQKQLLTIARALLADPKILILDEATSSIDTRTEVLIQKAMENLMEGRT
                     SFIIAHRLSTIRDADLILVMKDGDIVEQGNHEELLKADGFYASLYNSQFEGADAS"
     gene            complement(6863..7072)
                     /locus_tag="CON69_RS25995"
                     /old_locus_tag="CON69_25990"
     CDS             complement(6863..7072)
                     /locus_tag="CON69_RS25995"
                     /old_locus_tag="CON69_25990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006093745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_003204410.1"
                     /translation="MKLPSAEYTLKPGSVAAGVVNKTINSPFAQFWQGSGYWDTATKV
                     KPSSTSTGAIKTDDLKLIRSDVLEK"
CONTIG      join(NVOF01000081.1:1..7277)
//
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