LOCUS NZ_NFAW02000036 6656 bp DNA linear CON 19-DEC-2024
DEFINITION Klebsiella pneumoniae strain CRK0078 CRK0078_NODE_70.ctg_1, whole
genome shotgun sequence.
ACCESSION NZ_NFAW02000036 NZ_NFAW02000000
VERSION NZ_NFAW02000036.1
DBLINK BioProject: PRJNA224116
BioSample: SAMN06812596
Assembly: GCF_002186215.2
KEYWORDS WGS; RefSeq.
SOURCE Klebsiella pneumoniae
ORGANISM Klebsiella pneumoniae
Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
Enterobacterales; Enterobacteriaceae; Klebsiella/Raoultella group;
Klebsiella.
REFERENCE 1 (bases 1 to 6656)
AUTHORS Vanduin,D., Fouts,D., Wright,M., Sutton,G., Nguyen,K.,
Kreiswirth,B., Chen,L., Rojas,L., Hujer,A., Hujer,K., Bonomo,R. and
Adams,M.
TITLE Direct Submission
JOURNAL Submitted (18-OCT-2018) Informatics Core Services, JCVI, 9605
Medical Center Drive, Rockville, MD 20850, USA
REFERENCE 2 (bases 1 to 6656)
AUTHORS Fouts,D., Wright,M., Sutton,G., Nguyen,K., Kreiswirth,B., Chen,L.,
vanDuin,D., Rojas,L., Hujer,A., Hujer,K., Bonomo,R. and Adams,M.
TITLE Direct Submission
JOURNAL Submitted (02-MAY-2017) JCVI, 9704 Medical Center Drive, Rockville,
MD 20850, USA
COMMENT REFSEQ INFORMATION: The reference sequence is identical to
NFAW02000036.1.
The annotation was added by the NCBI Prokaryotic Genome Annotation
Pipeline (PGAP). Information about PGAP can be found here:
https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
##Genome-Assembly-Data-START##
Assembly Method :: SPAdes v. 3.10.0
Genome Representation :: Full
Expected Final Version :: Yes
Genome Coverage :: 116.85x
Sequencing Technology :: Illumina NextSeq 500
##Genome-Assembly-Data-END##
##Genome-Annotation-Data-START##
Annotation Provider :: NCBI RefSeq
Annotation Name :: GCF_002186215.2-RS_2024_12_19
Annotation Date :: 12/19/2024 01:29:06
Annotation Pipeline :: NCBI Prokaryotic Genome
Annotation Pipeline (PGAP)
Annotation Method :: Best-placed reference protein
set; GeneMarkS-2+
Annotation Software revision :: 6.9
Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA
Genes (total) :: 5,558
CDSs (total) :: 5,479
Genes (coding) :: 5,325
CDSs (with protein) :: 5,325
Genes (RNA) :: 79
rRNAs :: 2, 2, 1 (5S, 16S, 23S)
complete rRNAs :: 2, 1, 1 (5S, 16S, 23S)
partial rRNAs :: 1 (16S)
tRNAs :: 62
ncRNAs :: 12
Pseudo Genes (total) :: 154
CDSs (without protein) :: 154
Pseudo Genes (ambiguous residues) :: 0 of 154
Pseudo Genes (frameshifted) :: 49 of 154
Pseudo Genes (incomplete) :: 111 of 154
Pseudo Genes (internal stop) :: 13 of 154
Pseudo Genes (multiple problems) :: 16 of 154
##Genome-Annotation-Data-END##
FEATURES Location/Qualifiers
source 1..6656
/organism="Klebsiella pneumoniae"
/mol_type="genomic DNA"
/submitter_seqid="CRK0078_NODE_70.ctg_1"
/strain="CRK0078"
/isolation_source="blood"
/host="Homo sapiens"
/db_xref="taxon:573"
/geo_loc_name="USA: Ohio"
/collection_date="2013"
/collected_by="David van Duin"
gene <1..1755
/gene="traG"
/locus_tag="B9027_RS07415"
/old_locus_tag="B9027_007415"
CDS <1..1755
/gene="traG"
/locus_tag="B9027_RS07415"
/old_locus_tag="B9027_007415"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_020326930.1"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="conjugal transfer mating-pair stabilization
protein TraG"
/protein_id="WP_058876160.1"
/translation="WLQTWPLLFAILNSAMAYYAKQNGVPVVLSELSQVQLKNSDIAT
TAGYIAVMIPPLSWGIVKSMGAAFSSAYSHFSSSGLGATSQAASGVVDGNYSFANMQM
ENVSGYSWGTNSTTSFGQMSRQLANGGMSTQTRDGSMVWDSGGAMSKLPVDINVGRQI
ASAQQQMAREADVQAESALHGYNSSVTSAWNVLKQFGTNRGTSSSMTTGADNTESSQD
TMAKSKMWNAVVANAKANNISNEQSFQNLMDESTRGTVTGEAHVGARWSSGDQLAGKI
GKLATGASVEGGIKGSISGSGSTGSTDTVGQSGRHSSDSRHDISSQAAKDFREASDYL
VSHKTNTSGNITDNNASSRVDQFAASLSSAKNSYDQYTTSRTRSHEYSEMASRTESLT
GQMSENLTQQFANFVQHRAPQDAEAILTNTSSPEIAAQREMLAREFVKAQVEPRIDSS
FEQGRNDIGNGMASVSGTPGDQTVVSDFGKHSGDIESMSRNSGIKENVRNQVDSMVED
NTRINNAGKINIKEQGEGIKQQNADFEMLHKKEGNDFNERYNKELNGQVNNPLSNTTE
EFRKKAEESARKMDGKLP"
gene 1768..2316
/gene="traS"
/locus_tag="B9027_RS07420"
/old_locus_tag="B9027_007420"
CDS 1768..2316
/gene="traS"
/locus_tag="B9027_RS07420"
/old_locus_tag="B9027_007420"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_004152623.1"
/note="TraS, as the term is used in F-like plasmids, is a
plasmid entry exclusion protein. It interacts with the
F-like plasmid mating stabilization protein TraG; Derived
by automated computational analysis using gene prediction
method: Protein Homology."
/codon_start=1
/transl_table=11
/product="conjugal transfer entry exclusion protein TraS"
/protein_id="WP_004152623.1"
/translation="MITNKQILTEVEQLSRLFKDGEADIPSMWNCFWPGLAAMFWLLL
WPIAMFSYKLFFVELPNAAQMGIAASVLVAFVAGLFMMLYIVNFRALYLSIPDTYRKQ
SALCQFIVGKAKFYCRVYLLTYVFLIISCTYPVYGAIYSTVLFICFSFCLMQIVNVDL
SRYQLTALTSLLETFKTSKSEK"
gene 2500..3231
/gene="traT"
/locus_tag="B9027_RS07425"
/old_locus_tag="B9027_007425"
CDS 2500..3231
/gene="traT"
/locus_tag="B9027_RS07425"
/old_locus_tag="B9027_007425"
/inference="COORDINATES: similar to AA
sequence:RefSeq:YP_005229690.1"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="conjugal transfer complement resistance protein
TraT"
/protein_id="WP_004152622.1"
/translation="MQFNKLMTVMVVSSALALSGCSAMGTAIKKRNLEVKTQMSETIW
LEPSNNKTVYLQIKNTSDKDMSGLQAKIASAVTSKGYQVVSNPDTAGYWIQANVLKAD
KMDLRESQGWLSRGYEGAVTGAALGAGITAYNSSSAGATLGVGLAAGLVGMAADAMVE
DVNYTMITDVQIAERTKTQVQTDNVAVLRQGTSGAKVQTSTETGNQHKYQTRVVSNAN
KVNLKFPEAQPVLEDQLAKSIANIL"
gene 3423..4112
/locus_tag="B9027_RS28470"
CDS 3423..4112
/locus_tag="B9027_RS28470"
/inference="COORDINATES: similar to AA
sequence:RefSeq:YP_005229689.1"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="hypothetical protein"
/protein_id="WP_004198206.1"
/translation="MTISEPVRSLDTHELMPGDIYPHPAVGESYSLSHCEVCSLFSDH
FMYICSALVILLLVFIAGVFFRKLMTSSTGRKRDTVSVIPDTKPEAQSKPEYFHLSIC
VEGPASTDDSALSSLASRVVREAENLYIPGVNRRDSADDVFAWSVQTQFPSDRLFGTR
IFPSTGWFERPDMIKPPVKCYCVCLQYTAETLPSTLHALVDEAGRQVAKSYIKGGHCP
GSEGYSFCITS"
gene 4241..6553
/gene="traD"
/locus_tag="B9027_RS07435"
/old_locus_tag="B9027_007435"
CDS 4241..6553
/gene="traD"
/locus_tag="B9027_RS07435"
/old_locus_tag="B9027_007435"
/inference="COORDINATES: similar to AA
sequence:RefSeq:WP_004152630.1"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="type IV conjugative transfer system coupling
protein TraD"
/protein_id="WP_004152620.1"
/translation="MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLM
LMYRLSWQTFVNGCVYWWCTTLGPMRDIIRSQPVYTIQYYGQSLEYTSEQILADKYTI
WCGEQLWTGFAFAAVVSLVICIVTFFIASWVLGRQGKQQSEDENTGGRQLSDKPKEVA
RQMKRDGVASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVRARGDMAII
YDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDP
FWQGSGRTIFAEGAYLMREDDDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIE
KTAISIRAVLTNYVKAIRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHA
SLKPVISMWLSIAIRGLLAMGENRNRRVWIFADELPTLHKLPDLVEILPEARKFGGCY
VFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIAEFAAGEIGEKEILKASEQ
YSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLALKYKPRP
KIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDTPEPGPADTDSHASEQPE
PVSPPAPAVMTVTPAPVKSPPTTKRPAAEPSVRATEPPVLRGTTVPLIKPKAAAAATA
ASTASSAGAPAAAAGGTEQELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQT
QRDMQRREEVNINHSHRHDEQDDVEIGGNF"
gene 6553..>6656
/locus_tag="B9027_RS29425"
CDS 6553..>6656
/locus_tag="B9027_RS29425"
/inference="COORDINATES: similar to AA
sequence:RefSeq:YP_005229687.1"
/note="Derived by automated computational analysis using
gene prediction method: Protein Homology."
/codon_start=1
/transl_table=11
/product="hypothetical protein"
/protein_id="WP_004208632.1"
/translation="MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQG"
CONTIG join(NFAW02000036.1:1..6656)
//