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Bacillus thuringiensis strain CH_65 contig00134, whole genome shotgun sequence

NCBI Reference Sequence: NZ_JAIVBL010000134.1

FASTA Graphics 

LOCUS       NZ_JAIVBL010000134      8688 bp    DNA     linear   CON 02-AUG-2024
DEFINITION  Bacillus thuringiensis strain CH_65 contig00134, whole genome
            shotgun sequence.
ACCESSION   NZ_JAIVBL010000134 NZ_JAIVBL010000000
VERSION     NZ_JAIVBL010000134.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21214355
            Assembly: GCF_020775435.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus thuringiensis
  ORGANISM  Bacillus thuringiensis
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales; Bacillaceae;
            Bacillus; Bacillus cereus group.
REFERENCE   1  (bases 1 to 8688)
  AUTHORS   Biggel,M.
  TITLE     Whole genome sequencing reveals that B. thuringiensis in foods
            originate from biopesticide residues
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8688)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2021) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     REFSEQ INFORMATION: The reference sequence is identical to
            JAIVBL010000134.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.14.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 30x
            Sequencing Technology  :: Illumina MiniSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_020775435.1-RS_2024_08_02
            Annotation Date                   :: 08/02/2024 05:24:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,805
            CDSs (total)                      :: 6,677
            Genes (coding)                    :: 6,302
            CDSs (with protein)               :: 6,302
            Genes (RNA)                       :: 128
            rRNAs                             :: 12, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 8, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 101
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 375
            CDSs (without protein)            :: 375
            Pseudo Genes (ambiguous residues) :: 0 of 375
            Pseudo Genes (frameshifted)       :: 119 of 375
            Pseudo Genes (incomplete)         :: 263 of 375
            Pseudo Genes (internal stop)      :: 92 of 375
            Pseudo Genes (multiple problems)  :: 77 of 375
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8688
                     /organism="Bacillus thuringiensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="contig00134"
                     /strain="CH_65"
                     /isolation_source="food"
                     /db_xref="taxon:1428"
                     /collection_date="2016"
     gene            107..472
                     /locus_tag="K8Z43_RS31855"
                     /old_locus_tag="K8Z43_31865"
     CDS             107..472
                     /locus_tag="K8Z43_RS31855"
                     /old_locus_tag="K8Z43_31865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_228236713.1"
                     /translation="MDTLWNCLERGVVMDTLWNCLERGVVMDTLWNCLERGVVMDLRT
                     YYENVINELESILLSSEKVCEDNFLKIEQYLSNKSFTENDIDYIFYHLDNLECLVKDR
                     VFLEDGLKRYREKLDKVKK"
     gene            566..2938
                     /locus_tag="K8Z43_RS31860"
                     /old_locus_tag="K8Z43_31870"
     CDS             566..2938
                     /locus_tag="K8Z43_RS31860"
                     /old_locus_tag="K8Z43_31870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162327.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="WP_088065227.1"
                     /translation="MALDIAKCGRRTIALREKATGDVMIRRMSCNQLSCPVCGPVIAA
                     RLQLRIKYFSQHERLFFFNTITSKSGLDDLEKIFTKIRRELSVHFTIDGYMKKKKVSH
                     EKALKWYEKKKKKMIDFDVDVELAIYSRKNAIIEVANSKKVFYKRLNVNQKIEFHKKY
                     NHLVDKLFPSIYETNKQDENLHKQLYERISRRFEENEFEDFKFIRVIELHESGQPHYH
                     FLCNRYVSHYLMKKITVEGVSEVYDNTYIVDHAMERNKILTYDTVDTDIVSFYVSKVT
                     RYVTKETIETFSELEKEGDIAKKLISSSNCIKLFDEKDEEESKYKKLGVYDYQINATS
                     YEVPKVLENSVEDFIKFHSVMLRDQYAIDLLKFSKSLDLEDFIKCNYVIHERLIQVSV
                     AEYKNKLSTVFDETDLTEEQLELLKSFVANKITLLLGRAGTGKTYTLVNLLKKLKPNP
                     RTTFILTYTGKASVRIKELLSQHELTEYKPMTVHKACSSNWNSNFLKNEYNNLECEYL
                     IIDEVGMIPRDILMKVLLAVPSYTKIIFAGDDAQLPPVNDSSVIPELEGLDFINTIRL
                     TKVFRSGDTVLELAYKVLNRELIDFRLYDEKQLEKLVLPLVEQGYQILTNTKKMTKKI
                     NLIVQANKDKITKCFNDYCYEIGDRVMIIANDSSRRVYNGDTGKIVDHDDKGILIQLD
                     FEDRQVFYRYLDTVNIVPAYAFTVHKSQGSEYENVAVFLESQPKLNTNNLLYTGITRA
                     KKNIKVYVPDEKVLSTMINCVPEPVSNITLNNVFDLVNGILDIKKTSSMG"
     gene            3077..4096
                     /locus_tag="K8Z43_RS31865"
                     /old_locus_tag="K8Z43_31875"
     CDS             3077..4096
                     /locus_tag="K8Z43_RS31865"
                     /old_locus_tag="K8Z43_31875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014893150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3895 domain-containing protein"
                     /protein_id="WP_042991743.1"
                     /translation="MYGAYLDPEDRDRIMGSLSDEQQEYLRIFLKRGNQTAFSSVLAR
                     LKGGESEKDAEHISTQWMFIEYIDAGKVSKDHPCECGRPLRYQYIVKNLVTNKILKFS
                     KDHFKKHTGLPSSIVKAVLKGMYKIDCELDEILIKISSGWSLFENLGMDSIPNDLIIP
                     IDIREHLELDLPLLERQVMRLKGDLRSYNSLKKNIVEKNDSVDFEINEEREEGQASLF
                     DEDIVFGFNLDDDNVQTIRSQPVKVKPVSIEKNISYQFLSFVEQEFILQFIQNVNSVS
                     VRQLCEFMIKEVGSLDKRYLTGKPHIYVHVCSYLDFLVTQGKLSYIENLDYIDRIYYI
                     ESDIS"
     gene            complement(4128..4268)
                     /locus_tag="K8Z43_RS31870"
                     /old_locus_tag="K8Z43_31880"
     CDS             complement(4128..4268)
                     /locus_tag="K8Z43_RS31870"
                     /old_locus_tag="K8Z43_31880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_170951690.1"
                     /translation="MTDKKERVEMRIPQSILKKVDEYKGENGISTRTATILELIRKGL
                     NK"
     gene            4379..4630
                     /locus_tag="K8Z43_RS31875"
                     /old_locus_tag="K8Z43_31885"
     CDS             4379..4630
                     /locus_tag="K8Z43_RS31875"
                     /old_locus_tag="K8Z43_31885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000888627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000888627.1"
                     /translation="MLEDKFLESKIKKLTNQNVVVFTDDEIIEGVLLDKDTGEERLFE
                     VFRKIWGKNFAWFGEGIYIQCEDTVTFRDYDEVDNIFKK"
     gene            6770..7303
                     /locus_tag="K8Z43_RS31880"
                     /old_locus_tag="K8Z43_31890"
     CDS             6770..7303
                     /locus_tag="K8Z43_RS31880"
                     /old_locus_tag="K8Z43_31890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3967 domain-containing protein"
                     /protein_id="WP_042991737.1"
                     /translation="MVVIYQTTKQAAQTLNINHTTFKKYYGMFERYNGYNFLRDSKGQ
                     VMFSEYDLEVFKRLLSVKAEPGRTIEESCRVVGEEFGILNKNQVITDISPENGGDNKA
                     IEELKELILMQNNKIDELTIKLNEQSNQTKMIETSVGDRDQQLVRLMKEMLEVKRMVA
                     ASNKKRWWHFLKDKFSR"
     gene            7559..7864
                     /locus_tag="K8Z43_RS31885"
                     /old_locus_tag="K8Z43_31895"
     CDS             7559..7864
                     /locus_tag="K8Z43_RS31885"
                     /old_locus_tag="K8Z43_31895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014893149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_042991738.1"
                     /translation="MENETIQEKNKRRRVPVRFSEITAQKLDDMSERYGMSVSALVNF
                     IVGQWLDNNYDIKDYTREKMIPAMMENVEPQMESMLQNPLIAGMFKEMAEQFVKASK"
     gene            7878..8120
                     /locus_tag="K8Z43_RS31890"
                     /old_locus_tag="K8Z43_31900"
     CDS             7878..8120
                     /locus_tag="K8Z43_RS31890"
                     /old_locus_tag="K8Z43_31900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_042991740.1"
                     /translation="MEELLTKILEKLDAVDKKVDKVSGDVQELKQELKAHRMEIDDNF
                     TKLYKLVEDKDVEIDVLNKRVFKNETTVEKLKRQKH"
     gene            8191..8544
                     /locus_tag="K8Z43_RS31895"
                     /old_locus_tag="K8Z43_31905"
     CDS             8191..8544
                     /locus_tag="K8Z43_RS31895"
                     /old_locus_tag="K8Z43_31905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_088065226.1"
                     /translation="MQNIKEIKKLIRKNPGKCASISTTEDGDFEGVLLSGFFAKQVMK
                     LASQSNHISHDFNSNDLRSSDLNLKGVFLFNGTGVHFVFYEGLESIRIYDSPMFEEDS
                     KEMFEYFKEWLHAHS"
CONTIG      join(JAIVBL010000134.1:1..8688)
//
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