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NCBI Reference Sequence: NZ_JAIVBL010000134.1
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LOCUS NZ_JAIVBL010000134 8688 bp DNA linear CON 02-AUG-2024 DEFINITION Bacillus thuringiensis strain CH_65 contig00134, whole genome shotgun sequence. ACCESSION NZ_JAIVBL010000134 NZ_JAIVBL010000000 VERSION NZ_JAIVBL010000134.1 DBLINK BioProject: PRJNA224116 BioSample: SAMN21214355 Assembly: GCF_020775435.1 KEYWORDS WGS; RefSeq. SOURCE Bacillus thuringiensis ORGANISM Bacillus thuringiensis Bacteria; Bacillati; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus; Bacillus cereus group. REFERENCE 1 (bases 1 to 8688) AUTHORS Biggel,M. TITLE Whole genome sequencing reveals that B. thuringiensis in foods originate from biopesticide residues JOURNAL Unpublished REFERENCE 2 (bases 1 to 8688) AUTHORS Biggel,M. TITLE Direct Submission JOURNAL Submitted (06-SEP-2021) Institute for Food Safety and Hygiene, University of Zurich, Winterthurerstrasse 272, Zurich 8057, Switzerland COMMENT REFSEQ INFORMATION: The reference sequence is identical to JAIVBL010000134.1. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Assembly-Data-START## Assembly Method :: SPAdes v. 3.14.1 Genome Representation :: Full Expected Final Version :: Yes Genome Coverage :: 30x Sequencing Technology :: Illumina MiniSeq ##Genome-Assembly-Data-END## ##Genome-Annotation-Data-START## Annotation Provider :: NCBI RefSeq Annotation Name :: GCF_020775435.1-RS_2024_08_02 Annotation Date :: 08/02/2024 05:24:15 Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline (PGAP) Annotation Method :: Best-placed reference protein set; GeneMarkS-2+ Annotation Software revision :: 6.7 Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total) :: 6,805 CDSs (total) :: 6,677 Genes (coding) :: 6,302 CDSs (with protein) :: 6,302 Genes (RNA) :: 128 rRNAs :: 12, 5, 5 (5S, 16S, 23S) complete rRNAs :: 4 (5S) partial rRNAs :: 8, 5, 5 (5S, 16S, 23S) tRNAs :: 101 ncRNAs :: 5 Pseudo Genes (total) :: 375 CDSs (without protein) :: 375 Pseudo Genes (ambiguous residues) :: 0 of 375 Pseudo Genes (frameshifted) :: 119 of 375 Pseudo Genes (incomplete) :: 263 of 375 Pseudo Genes (internal stop) :: 92 of 375 Pseudo Genes (multiple problems) :: 77 of 375 CRISPR Arrays :: 1 ##Genome-Annotation-Data-END## FEATURES Location/Qualifiers source 1..8688 /organism="Bacillus thuringiensis" /mol_type="genomic DNA" /submitter_seqid="contig00134" /strain="CH_65" /isolation_source="food" /db_xref="taxon:1428" /collection_date="2016" gene 107..472 /locus_tag="K8Z43_RS31855" /old_locus_tag="K8Z43_31865" CDS 107..472 /locus_tag="K8Z43_RS31855" /old_locus_tag="K8Z43_31865" /inference="COORDINATES: ab initio prediction:GeneMarkS-2+" /note="Derived by automated computational analysis using gene prediction method: GeneMarkS-2+." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="WP_228236713.1" /translation="MDTLWNCLERGVVMDTLWNCLERGVVMDTLWNCLERGVVMDLRT YYENVINELESILLSSEKVCEDNFLKIEQYLSNKSFTENDIDYIFYHLDNLECLVKDR VFLEDGLKRYREKLDKVKK" gene 566..2938 /locus_tag="K8Z43_RS31860" /old_locus_tag="K8Z43_31870" CDS 566..2938 /locus_tag="K8Z43_RS31860" /old_locus_tag="K8Z43_31870" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_001162327.1" /GO_function="GO:0003677 - DNA binding [Evidence IEA]" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="ATP-dependent RecD-like DNA helicase" /protein_id="WP_088065227.1" /translation="MALDIAKCGRRTIALREKATGDVMIRRMSCNQLSCPVCGPVIAA RLQLRIKYFSQHERLFFFNTITSKSGLDDLEKIFTKIRRELSVHFTIDGYMKKKKVSH EKALKWYEKKKKKMIDFDVDVELAIYSRKNAIIEVANSKKVFYKRLNVNQKIEFHKKY NHLVDKLFPSIYETNKQDENLHKQLYERISRRFEENEFEDFKFIRVIELHESGQPHYH FLCNRYVSHYLMKKITVEGVSEVYDNTYIVDHAMERNKILTYDTVDTDIVSFYVSKVT RYVTKETIETFSELEKEGDIAKKLISSSNCIKLFDEKDEEESKYKKLGVYDYQINATS YEVPKVLENSVEDFIKFHSVMLRDQYAIDLLKFSKSLDLEDFIKCNYVIHERLIQVSV AEYKNKLSTVFDETDLTEEQLELLKSFVANKITLLLGRAGTGKTYTLVNLLKKLKPNP RTTFILTYTGKASVRIKELLSQHELTEYKPMTVHKACSSNWNSNFLKNEYNNLECEYL IIDEVGMIPRDILMKVLLAVPSYTKIIFAGDDAQLPPVNDSSVIPELEGLDFINTIRL TKVFRSGDTVLELAYKVLNRELIDFRLYDEKQLEKLVLPLVEQGYQILTNTKKMTKKI NLIVQANKDKITKCFNDYCYEIGDRVMIIANDSSRRVYNGDTGKIVDHDDKGILIQLD FEDRQVFYRYLDTVNIVPAYAFTVHKSQGSEYENVAVFLESQPKLNTNNLLYTGITRA KKNIKVYVPDEKVLSTMINCVPEPVSNITLNNVFDLVNGILDIKKTSSMG" gene 3077..4096 /locus_tag="K8Z43_RS31865" /old_locus_tag="K8Z43_31875" CDS 3077..4096 /locus_tag="K8Z43_RS31865" /old_locus_tag="K8Z43_31875" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_014893150.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="DUF3895 domain-containing protein" /protein_id="WP_042991743.1" /translation="MYGAYLDPEDRDRIMGSLSDEQQEYLRIFLKRGNQTAFSSVLAR LKGGESEKDAEHISTQWMFIEYIDAGKVSKDHPCECGRPLRYQYIVKNLVTNKILKFS KDHFKKHTGLPSSIVKAVLKGMYKIDCELDEILIKISSGWSLFENLGMDSIPNDLIIP IDIREHLELDLPLLERQVMRLKGDLRSYNSLKKNIVEKNDSVDFEINEEREEGQASLF DEDIVFGFNLDDDNVQTIRSQPVKVKPVSIEKNISYQFLSFVEQEFILQFIQNVNSVS VRQLCEFMIKEVGSLDKRYLTGKPHIYVHVCSYLDFLVTQGKLSYIENLDYIDRIYYI ESDIS" gene complement(4128..4268) /locus_tag="K8Z43_RS31870" /old_locus_tag="K8Z43_31880" CDS complement(4128..4268) /locus_tag="K8Z43_RS31870" /old_locus_tag="K8Z43_31880" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_000130115.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="WP_170951690.1" /translation="MTDKKERVEMRIPQSILKKVDEYKGENGISTRTATILELIRKGL NK" gene 4379..4630 /locus_tag="K8Z43_RS31875" /old_locus_tag="K8Z43_31885" CDS 4379..4630 /locus_tag="K8Z43_RS31875" /old_locus_tag="K8Z43_31885" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_000888627.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="WP_000888627.1" /translation="MLEDKFLESKIKKLTNQNVVVFTDDEIIEGVLLDKDTGEERLFE VFRKIWGKNFAWFGEGIYIQCEDTVTFRDYDEVDNIFKK" gene 6770..7303 /locus_tag="K8Z43_RS31880" /old_locus_tag="K8Z43_31890" CDS 6770..7303 /locus_tag="K8Z43_RS31880" /old_locus_tag="K8Z43_31890" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_000260421.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="DUF3967 domain-containing protein" /protein_id="WP_042991737.1" /translation="MVVIYQTTKQAAQTLNINHTTFKKYYGMFERYNGYNFLRDSKGQ VMFSEYDLEVFKRLLSVKAEPGRTIEESCRVVGEEFGILNKNQVITDISPENGGDNKA IEELKELILMQNNKIDELTIKLNEQSNQTKMIETSVGDRDQQLVRLMKEMLEVKRMVA ASNKKRWWHFLKDKFSR" gene 7559..7864 /locus_tag="K8Z43_RS31885" /old_locus_tag="K8Z43_31895" CDS 7559..7864 /locus_tag="K8Z43_RS31885" /old_locus_tag="K8Z43_31895" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_014893149.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="WP_042991738.1" /translation="MENETIQEKNKRRRVPVRFSEITAQKLDDMSERYGMSVSALVNF IVGQWLDNNYDIKDYTREKMIPAMMENVEPQMESMLQNPLIAGMFKEMAEQFVKASK" gene 7878..8120 /locus_tag="K8Z43_RS31890" /old_locus_tag="K8Z43_31900" CDS 7878..8120 /locus_tag="K8Z43_RS31890" /old_locus_tag="K8Z43_31900" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_000342926.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="WP_042991740.1" /translation="MEELLTKILEKLDAVDKKVDKVSGDVQELKQELKAHRMEIDDNF TKLYKLVEDKDVEIDVLNKRVFKNETTVEKLKRQKH" gene 8191..8544 /locus_tag="K8Z43_RS31895" /old_locus_tag="K8Z43_31905" CDS 8191..8544 /locus_tag="K8Z43_RS31895" /old_locus_tag="K8Z43_31905" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_001179589.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="WP_088065226.1" /translation="MQNIKEIKKLIRKNPGKCASISTTEDGDFEGVLLSGFFAKQVMK LASQSNHISHDFNSNDLRSSDLNLKGVFLFNGTGVHFVFYEGLESIRIYDSPMFEEDS KEMFEYFKEWLHAHS" CONTIG join(JAIVBL010000134.1:1..8688) //
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