LOCUS NM_020708 6026 bp mRNA linear PRI 11-OCT-2024
DEFINITION Homo sapiens solute carrier family 12 member 5 (SLC12A5),
transcript variant 2, mRNA.
ACCESSION NM_020708
VERSION NM_020708.5
KEYWORDS RefSeq; MANE Select.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 6026)
AUTHORS Pearson,A.C. and Ostroumov,A.
TITLE Midbrain KCC2 downregulation: Implications for stress-related and
substance use behaviors
JOURNAL Curr Opin Neurobiol 88, 102901 (2024)
PUBMED 39142020
REMARK GeneRIF: Midbrain KCC2 downregulation: Implications for
stress-related and substance use behaviors.
Review article
REFERENCE 2 (bases 1 to 6026)
AUTHORS Kok,M., Hartnett-Scott,K., Happe,C.L., MacDonald,M.L., Aizenman,E.
and Brodsky,J.L.
TITLE The expression system influences stability, maturation efficiency,
and oligomeric properties of the potassium-chloride co-transporter
KCC2
JOURNAL Neurochem Int 174, 105695 (2024)
PUBMED 38373478
REMARK GeneRIF: The expression system influences stability, maturation
efficiency, and oligomeric properties of the potassium-chloride
co-transporter KCC2.
REFERENCE 3 (bases 1 to 6026)
AUTHORS Chen,J., Wang,H., Deng,C. and Fei,M.
TITLE SLC12A5 as a novel potential biomarker of glioblastoma multiforme
JOURNAL Mol Biol Rep 50 (5), 4285-4299 (2023)
PUBMED 36917367
REMARK GeneRIF: SLC12A5 as a novel potential biomarker of glioblastoma
multiforme.
REFERENCE 4 (bases 1 to 6026)
AUTHORS Tong,Q., Qin,W., Li,Z.H., Liu,C., Wang,Z.C., Chu,Y. and Xu,X.D.
TITLE SLC12A5 promotes hepatocellular carcinoma growth and ferroptosis
resistance by inducing ER stress and cystine transport changes
JOURNAL Cancer Med 12 (7), 8526-8541 (2023)
PUBMED 36645171
REMARK GeneRIF: SLC12A5 promotes hepatocellular carcinoma growth and
ferroptosis resistance by inducing ER stress and cystine transport
changes.
REFERENCE 5 (bases 1 to 6026)
AUTHORS Li,Y., Li,Y.L., Liu,Y.L., Fu,J., Zhang,W.W. and Piao,Y.S.
TITLE [Expression of cation chloride cotransporter (NKCC1/KCC2) in brain
tissue of children with focal cortical dysplasia type ]
JOURNAL Zhonghua Bing Li Xue Za Zhi 51 (11), 1123-1128 (2022)
PUBMED 36323541
REMARK GeneRIF: [Expression of cation chloride cotransporter (NKCC1/KCC2)
in brain tissue of children with focal cortical dysplasia type ].
REFERENCE 6 (bases 1 to 6026)
AUTHORS Hebert,S.C., Mount,D.B. and Gamba,G.
TITLE Molecular physiology of cation-coupled Cl- cotransport: the SLC12
family
JOURNAL Pflugers Arch 447 (5), 580-593 (2004)
PUBMED 12739168
REMARK Review article
REFERENCE 7 (bases 1 to 6026)
AUTHORS Brauer,M., Frei,E., Claes,L., Grissmer,S. and Jager,H.
TITLE Influence of K-Cl cotransporter activity on activation of
volume-sensitive Cl- channels in human osteoblasts
JOURNAL Am J Physiol Cell Physiol 285 (1), C22-C30 (2003)
PUBMED 12637262
REMARK GeneRIF: human osteoblasts express functional K-Cl cotransporters
in their cell membrane that seem to be able to induce the indirect
activation of volume-sensitive Cl- channels by KCl through an
increase in the intracellular ions, water influx and cell swelling.
REFERENCE 8 (bases 1 to 6026)
AUTHORS Song,L., Mercado,A., Vazquez,N., Xie,Q., Desai,R., George,A.L. Jr.,
Gamba,G. and Mount,D.B.
TITLE Molecular, functional, and genomic characterization of human KCC2,
the neuronal K-Cl cotransporter
JOURNAL Brain Res Mol Brain Res 103 (1-2), 91-105 (2002)
PUBMED 12106695
REMARK GeneRIF: Expression of hKCC2 cRNA in Xenopus laevis oocytes results
in significant Cl(-)-dependent (86)Rb(+) uptake under isotonic
conditions
REFERENCE 9 (bases 1 to 6026)
AUTHORS Sallinen,R., Tornberg,J., Putkiranta,M., Horelli-Kuitunen,N.,
Airaksinen,M.S. and Wessman,M.
TITLE Chromosomal localization of SLC12A5/Slc12a5, the human and mouse
genes for the neuron-specific K(+)-Cl(-) cotransporter (KCC2)
defines a new region of conserved homology
JOURNAL Cytogenet Cell Genet 94 (1-2), 67-70 (2001)
PUBMED 11701957
REMARK GeneRIF: chromosome 20 mapping
Erratum:[Cytogenet Cell Genet. 2002;98(2-3):232]
REFERENCE 10 (bases 1 to 6026)
AUTHORS McTague,A. and Kurian,M.A.
TITLE SLC12A5-Related Epilepsy of Infancy with Migrating Focal Seizures
JOURNAL (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
Amemiya A (Eds.);
GENEREVIEWS(R);
(1993)
PUBMED 30763027
COMMENT REVIEWED REFSEQ: This record has been curated by NCBI staff. The
reference sequence was derived from AL162458.10.
This sequence is a reference standard in the RefSeqGene project.
On Jun 2, 2019 this sequence version replaced NM_020708.4.
Summary: K-Cl cotransporters are proteins that lower intracellular
chloride concentrations below the electrochemical equilibrium
potential. The protein encoded by this gene is an integral membrane
K-Cl cotransporter that can function in either a net efflux or
influx pathway, depending on the chemical concentration gradients
of potassium and chloride. The encoded protein can act as a
homomultimer, or as a heteromultimer with other K-Cl
cotransporters, to maintain chloride homeostasis in neurons.
Alternative splicing results in two transcript variants encoding
different isoforms. [provided by RefSeq, Sep 2008].
Transcript Variant: This variant (2) differs in the 5' UTR and 5'
coding region, compared to variant 1. The resulting isoform (2,
also known as KCC2b) has a distinct N-terminus and is shorter than
isoform 1.
Publication Note: This RefSeq record includes a subset of the
publications that are available for this gene. Please see the Gene
record to access additional publications.
##Evidence-Data-START##
Transcript exon combination :: BC132670.1, AF208159.1 [ECO:0000332]
RNAseq introns :: single sample supports all introns
SAMEA2145743, SAMEA2154665
[ECO:0000348]
##Evidence-Data-END##
##RefSeq-Attributes-START##
MANE Ensembl match :: ENST00000243964.7/ ENSP00000243964.4
RefSeq Select criteria :: based on manual assertion, conservation,
expression
##RefSeq-Attributes-END##
COMPLETENESS: complete on the 3' end.
PRIMARY REFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP
1-182 AL162458.10 115631-115812
183-277 AL162458.10 121364-121458
278-409 AL162458.10 121820-121951
410-556 AL162458.10 122193-122339
557-611 AL162458.10 123157-123211
612-742 AL162458.10 123671-123801
743-984 AL162458.10 126789-127030
985-1196 AL162458.10 127745-127956
1197-1367 AL162458.10 129569-129739
1368-1466 AL162458.10 130049-130147
1467-1524 AL162458.10 130292-130349
1525-1699 AL162458.10 131382-131556
1700-1818 AL162458.10 132294-132412
1819-1917 AL162458.10 132754-132852
1918-2037 AL162458.10 133870-133989
2038-2142 AL162458.10 134397-134501
2143-2311 AL162458.10 136038-136206
2312-2507 AL162458.10 138091-138286
2508-2677 AL162458.10 139373-139542
2678-2809 AL162458.10 139994-140125
2810-2917 AL162458.10 141332-141439
2918-3040 AL162458.10 142566-142688
3041-3240 AL162458.10 142781-142980
3241-3255 AL162458.10 143313-143327
3256-3389 AL162458.10 143586-143719
3390-6026 AL162458.10 143930-146566
FEATURES Location/Qualifiers
source 1..6026
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/chromosome="20"
/map="20q13.12"
gene 1..6026
/gene="SLC12A5"
/gene_synonym="DEE34; EIEE34; EIG14; hKCC2; KCC2"
/note="solute carrier family 12 member 5"
/db_xref="GeneID:57468"
/db_xref="HGNC:HGNC:13818"
/db_xref="MIM:606726"
exon 1..182
/gene="SLC12A5"
/gene_synonym="DEE34; EIEE34; EIG14; hKCC2; KCC2"
/inference="alignment:Splign:2.1.0"
CDS 131..3481
/gene="SLC12A5"
/gene_synonym="DEE34; EIEE34; EIG14; hKCC2; KCC2"
/note="isoform 2 is encoded by transcript variant 2;
erythroid K-Cl cotransporter 2; neuronal K-Cl
cotransporter; solute carrier family 12
(potassium/chloride transporter), member 5; electroneutral
potassium-chloride cotransporter 2"
/codon_start=1
/product="solute carrier family 12 member 5 isoform 2"
/protein_id="NP_065759.1"
/db_xref="CCDS:CCDS13391.1"
/db_xref="GeneID:57468"
/db_xref="HGNC:HGNC:13818"
/db_xref="MIM:606726"
/translation="MLNNLTDCEDGDGGANPGDGNPKESSPFINSTDTEKGKEYDGKN
MALFEEEMDTSPMVSSLLSGLANYTNLPQGSREHEEAENNEGGKKKPVQAPRMGTFMG
VYLPCLQNIFGVILFLRLTWVVGIAGIMESFCMVFICCSCTMLTAISMSAIATNGVVP
AGGSYYMISRSLGPEFGGAVGLCFYLGTTFAGAMYILGTIEILLAYLFPAMAIFKAED
ASGEAAAMLNNMRVYGTCVLTCMATVVFVGVKYVNKFALVFLGCVILSILAIYAGVIK
SAFDPPNFPICLLGNRTLSRHGFDVCAKLAWEGNETVTTRLWGLFCSSRFLNATCDEY
FTRNNVTEIQGIPGAASGLIKENLWSSYLTKGVIVERSGMTSVGLADGTPIDMDHPYV
FSDMTSYFTLLVGIYFPSVTGIMAGSNRSGDLRDAQKSIPTGTILAIATTSAVYISSV
VLFGACIEGVVLRDKFGEAVNGNLVVGTLAWPSPWVIVIGSFFSTCGAGLQSLTGAPR
LLQAISRDGIVPFLQVFGHGKANGEPTWALLLTACICEIGILIASLDEVAPILSMFFL
MCYMFVNLACAVQTLLRTPNWRPRFRYYHWTLSFLGMSLCLALMFICSWYYALVAMLI
AGLIYKYIEYRGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLVRVDQ
DQNVVHPQLLSLTSQLKAGKGLTIVGSVLEGTFLENHPQAQRAEESIRRLMEAEKVKG
FCQVVISSNLRDGVSHLIQSGGLGGLQHNTVLVGWPRNWRQKEDHQTWRNFIELVRET
TAGHLALLVTKNVSMFPGNPERFSEGSIDVWWIVHDGGMLMLLPFLLRHHKVWRKCKM
RIFTVAQMDDNSIQMKKDLTTFLYHLRITAEVEVVEMHESDISAYTYEKTLVMEQRSQ
ILKQMHLTKNEREREIQSITDESRGSIRRKNPANTRLRLNVPEETAGDSEEKPEEEVQ
LIHDQSAPSCPSSSPSPGEEPEGEGETDPEKVHLTWTKDKSVAEKNKGPSPVSSEGIK
DFFSMKPEWENLNQSNVRRMHTAVRLNEVIVKKSRDAKLVLLNMPGPPRNRNGDENYM
EFLEVLTEHLDRVMLVRGGGREVITIYS"
exon 183..277
/gene="SLC12A5"
/gene_synonym="DEE34; EIEE34; EIG14; hKCC2; KCC2"
/inference="alignment:Splign:2.1.0"
exon 278..409
/gene="SLC12A5"
/gene_synonym="DEE34; EIEE34; EIG14; hKCC2; KCC2"
/inference="alignment:Splign:2.1.0"
exon 410..556
/gene="SLC12A5"
/gene_synonym="DEE34; EIEE34; EIG14; hKCC2; KCC2"
/inference="alignment:Splign:2.1.0"
exon 557..611
/gene="SLC12A5"
/gene_synonym="DEE34; EIEE34; EIG14; hKCC2; KCC2"
/inference="alignment:Splign:2.1.0"
exon 612..742
/gene="SLC12A5"
/gene_synonym="DEE34; EIEE34; EIG14; hKCC2; KCC2"
/inference="alignment:Splign:2.1.0"
exon 743..984
/gene="SLC12A5"
/gene_synonym="DEE34; EIEE34; EIG14; hKCC2; KCC2"
/inference="alignment:Splign:2.1.0"
exon 985..1196
/gene="SLC12A5"
/gene_synonym="DEE34; EIEE34; EIG14; hKCC2; KCC2"
/inference="alignment:Splign:2.1.0"
exon 1197..1367
/gene="SLC12A5"
/gene_synonym="DEE34; EIEE34; EIG14; hKCC2; KCC2"
/inference="alignment:Splign:2.1.0"
exon 1368..1466
/gene="SLC12A5"
/gene_synonym="DEE34; EIEE34; EIG14; hKCC2; KCC2"
/inference="alignment:Splign:2.1.0"
exon 1467..1524
/gene="SLC12A5"
/gene_synonym="DEE34; EIEE34; EIG14; hKCC2; KCC2"
/inference="alignment:Splign:2.1.0"
exon 1525..1699
/gene="SLC12A5"
/gene_synonym="DEE34; EIEE34; EIG14; hKCC2; KCC2"
/inference="alignment:Splign:2.1.0"
exon 1700..1818
/gene="SLC12A5"
/gene_synonym="DEE34; EIEE34; EIG14; hKCC2; KCC2"
/inference="alignment:Splign:2.1.0"
exon 1819..1917
/gene="SLC12A5"
/gene_synonym="DEE34; EIEE34; EIG14; hKCC2; KCC2"
/inference="alignment:Splign:2.1.0"
exon 1918..2037
/gene="SLC12A5"
/gene_synonym="DEE34; EIEE34; EIG14; hKCC2; KCC2"
/inference="alignment:Splign:2.1.0"
exon 2038..2142
/gene="SLC12A5"
/gene_synonym="DEE34; EIEE34; EIG14; hKCC2; KCC2"
/inference="alignment:Splign:2.1.0"
exon 2143..2311
/gene="SLC12A5"
/gene_synonym="DEE34; EIEE34; EIG14; hKCC2; KCC2"
/inference="alignment:Splign:2.1.0"
exon 2312..2507
/gene="SLC12A5"
/gene_synonym="DEE34; EIEE34; EIG14; hKCC2; KCC2"
/inference="alignment:Splign:2.1.0"
exon 2508..2677
/gene="SLC12A5"
/gene_synonym="DEE34; EIEE34; EIG14; hKCC2; KCC2"
/inference="alignment:Splign:2.1.0"
exon 2678..2809
/gene="SLC12A5"
/gene_synonym="DEE34; EIEE34; EIG14; hKCC2; KCC2"
/inference="alignment:Splign:2.1.0"
exon 2810..2917
/gene="SLC12A5"
/gene_synonym="DEE34; EIEE34; EIG14; hKCC2; KCC2"
/inference="alignment:Splign:2.1.0"
exon 2918..3040
/gene="SLC12A5"
/gene_synonym="DEE34; EIEE34; EIG14; hKCC2; KCC2"
/inference="alignment:Splign:2.1.0"
exon 3041..3240
/gene="SLC12A5"
/gene_synonym="DEE34; EIEE34; EIG14; hKCC2; KCC2"
/inference="alignment:Splign:2.1.0"
exon 3241..3255
/gene="SLC12A5"
/gene_synonym="DEE34; EIEE34; EIG14; hKCC2; KCC2"
/inference="alignment:Splign:2.1.0"
exon 3256..3389
/gene="SLC12A5"
/gene_synonym="DEE34; EIEE34; EIG14; hKCC2; KCC2"
/inference="alignment:Splign:2.1.0"
exon 3390..6026
/gene="SLC12A5"
/gene_synonym="DEE34; EIEE34; EIG14; hKCC2; KCC2"
/inference="alignment:Splign:2.1.0"
regulatory 6001..6006
/regulatory_class="polyA_signal_sequence"
/gene="SLC12A5"
/gene_synonym="DEE34; EIEE34; EIG14; hKCC2; KCC2"
/note="hexamer: AATAAA"
polyA_site 6026
/gene="SLC12A5"
/gene_synonym="DEE34; EIEE34; EIG14; hKCC2; KCC2"
/note="major polyA site"
ORIGIN
1 actgcagctt cttcctccgt ggagcggaga gcgagacaga gctacagcga acgagagagc
61 ggcgaaggcg ggtagagggg cgcgggcgag gcggcgcagc catccccgga ccaggggccg
121 cgccgccacc atgctaaaca acctgacgga ctgcgaggac ggcgatgggg gagccaaccc
181 gggtgatggc aaccccaagg aaagcagtcc cttcatcaac agcaccgaca cagagaaggg
241 aaaggagtat gatggcaaga acatggcctt gtttgaggag gagatggaca ccagccctat
301 ggtgtcctcc ttgctcagtg gcctggccaa ctacaccaac ctgccccagg gaagtaggga
361 gcatgaagag gcagaaaaca atgagggtgg aaaaaagaag ccggtgcagg ccccacgcat
421 gggcaccttc atgggcgtgt acctgccgtg cctgcagaac atctttggcg tcatcctctt
481 cctgcggctc acctgggtgg tgggcattgc aggcatcatg gagtccttct gcatggtgtt
541 catctgctgc tcctgtacga tgctcacggc catctccatg agtgcaattg caacgaatgg
601 tgttgtgcct gctggtggct cctactacat gatttccagg tctctgggcc cagagtttgg
661 gggtgccgtg ggcctctgct tctacctggg cactaccttt gcaggagcca tgtacatcct
721 gggcaccatc gaaatcctgc tggcttacct cttcccagcc atggccatct tcaaggcaga
781 agatgccagt ggggaggcag cagccatgct gaacaacatg cgtgtttacg gcacctgtgt
841 gctcacctgc atggccactg tggtgtttgt gggtgtcaag tatgtcaaca agtttgccct
901 tgtcttcctg ggttgtgtca tcctctccat cctggccatc tatgctgggg tcatcaagtc
961 tgccttcgac ccacccaact tcccgatctg cctcctgggt aaccgcacgc tgtctcgcca
1021 tggctttgat gtctgtgcca agctggcttg ggaaggaaat gagacggtga ccacacggct
1081 atggggcctt ttctgctcct ctcgcttcct caacgccacc tgtgatgaat acttcacccg
1141 aaacaatgtc acagagatcc agggcatccc tggtgctgcc agtggcctca tcaaagagaa
1201 cctctggagc tcctacctga ccaagggcgt gattgtggag aggagtggga tgacctcggt
1261 gggcctggcc gatggcactc ctatcgacat ggaccaccct tatgtcttca gtgatatgac
1321 ctcctacttc accctgctgg ttggcatcta cttcccctca gtcacaggga tcatggctgg
1381 ttctaaccgc tctggggacc tgagggatgc ccagaagtca atccccactg gcaccatcct
1441 ggccatcgcc accacctctg ctgtctacat cagctccgtt gttctgtttg gggcctgcat
1501 tgagggggtc gtcctgcggg acaagtttgg cgaagctgtg aatggcaacc tcgtggtggg
1561 cactctggcc tggccatctc catgggtaat tgtcatcgga tccttcttct ccacctgtgg
1621 ggctgggctg cagagcctca cgggggcccc acgcctgctg caggccatct cgagggatgg
1681 cattgtgccc ttcctgcagg tctttggcca tggcaaggcc aatggagagc cgacctgggc
1741 cctgctcctg actgcctgca tctgcgagat tggcatcctc attgcatccc tcgacgaggt
1801 ggcccccatc ctctctatgt tcttcctgat gtgctacatg tttgtgaatc tggcctgtgc
1861 agtgcagacg ctgctgagga cacccaactg gaggccacgc tttcgatatt accactggac
1921 cctctccttc ctgggcatga gcctctgcct ggccctcatg ttcatctgct cctggtatta
1981 tgcactggta gccatgctca ttgctggact catctacaag tacattgagt accgtggggc
2041 agagaaggag tggggcgatg ggatacgagg tctgtctctc agtgcggctc gctatgccct
2101 cttacgcctg gaggaagggc ccccacacac caagaactgg aggccacagc tgctggtgct
2161 ggtgcgtgtg gaccaagacc agaatgtggt gcacccccag ctgctctcac tgacctccca
2221 gctgaaggcg gggaagggcc tgaccatcgt gggctctgtc cttgagggca cctttctgga
2281 aaatcatcca caggcccagc gggcagaaga gtctatcagg cgcctgatgg aggcagagaa
2341 ggtgaagggc ttctgccagg tggtgatctc ctccaacttg cgtgatggcg tgtcccatct
2401 gatccagtcc gggggcctcg gggggctgca gcacaacact gtgcttgttg gctggccccg
2461 caactggcgc cagaaggaag atcatcagac gtggaggaac ttcattgagc tggtccggga
2521 aaccacagct ggccacttag ccctgctggt caccaagaac gtttccatgt ttcctgggaa
2581 ccctgagcgc ttctctgagg gcagcatcga cgtttggtgg attgtgcacg atggaggcat
2641 gctcatgctg ctgcccttcc tgctgcggca ccacaaggtc tggcggaagt gcaagatgcg
2701 tatcttcact gtggcccaga tggatgacaa tagcatccag atgaagaagg atctgaccac
2761 atttctgtat catttacgca tcactgcgga ggtcgaggtg gtggagatgc atgagagcga
2821 catctcagct tacacctatg agaagacgtt ggtgatggag cagcgttccc agatcctcaa
2881 acagatgcat ttaaccaaga atgagcggga gcgggagatc cagagtatca cagatgagtc
2941 acgaggctca atccggagaa agaatccagc caacacgcgg ctccgcctga acgtcccaga
3001 agagacggct ggtgacagtg aagagaagcc agaggaggag gtgcagctga tccacgatca
3061 gagtgctccc agctgcccca gcagctcccc gtccccaggg gaggagcctg agggggaagg
3121 ggagacagat ccggagaagg tgcatctcac ctggaccaag gacaagtcgg tggcagagaa
3181 gaataagggc cccagtcctg tctcctctga gggcatcaag gacttcttca gcatgaagcc
3241 ggagtgggag aacttgaacc agtccaacgt gcggcgcatg cacacggccg tgcggctgaa
3301 cgaggtcatc gtgaagaaat cccgggacgc caagcttgtt ttgctcaaca tgcctgggcc
3361 tccccgcaac cgcaatggtg atgaaaacta catggagttt ctcgaggtcc tcacagagca
3421 cctggaccgg gtgatgctgg tccgcggcgg cggccgcgag gtcatcacca tctactcctg
3481 agaaccagga cctgccaccc gggcccgagc gcgcccggcc cgcggctccg gagccctcgc
3541 cgcgcccccc gccgctgtca ccgtttacat acagaccctg tgcccgtgtc ctggcccctt
3601 accccgctgc ctgaagcccg gaggccacgc ctgttggggc tgattcggag agggcgcccc
3661 gccgcgcaga gaccagagct cctcagtgcc agtttggccc ctgggtcttc gctgcccttt
3721 ttctaagccc ggcctcgtct cgccggagga gacgctgcaa taaaggttgg gagaaggcgc
3781 ggaaaggaga ggagctgggg ccttggggac ccccaggtag tccatgcggc ccattcctcc
3841 ccttcccact cccgccgcgg tcctcgctct gcgctcctcc ggcgctgctc cctggctccc
3901 ggcggcccgg aggcccgcgg ggtgggaagg ccgcgcttgc cgtctccgcc gccccttctc
3961 gccgagccgt ggggcgcggg cggccgagcc tatacatagt gtacaggaga catcgcgtgt
4021 atttttaacg tccccatatt tatgtgacta gaagcgcaac agacttctcg ccatagtcga
4081 gctctcccgc tgggggcact gcggggaggc gaggcctcgg gaagctgaat tttccttgac
4141 gtccaagagt ttgagagcga aagtgcttta ggcccaggcg ggggtcgtgg cctcgttccc
4201 tcgacacctc cgtcctgctc tcgcctcttc gccctttccg cgcgcccttg gcttcccacc
4261 ctcctctcca gtccttttcc gagatgaggt gagacaaggg tccaactttt cctggattcg
4321 cctcccagcg gacgtgagct tccactgcgg ctgcagagac gcgagcaacc tcttctcatc
4381 ggctcttatg caagttgggg ccaggatagg ggaggggtgc tcctcaagag gaagaaaccg
4441 agaggcccgc gccccaccga ggaagccccg ccccggtgcc ttcgctgggg agcaggcgtc
4501 tctcctcagt cggcttgtcg cctgctcccc gtatcccatg gctcctcgcc aaagactgaa
4561 attgtggagc tggagggcgc cccctccccg gagtttcctc cctgggacaa gtgagggagg
4621 agggggccga ttctggttta ggggccggac ccactgagag gccccagagc cgcccgtgat
4681 gttcctcccc cgtccccatc tggcagctcc tgtctcgcct gagggaccca gccgccttct
4741 ccgtgctctg gggccgggcc tcgctgctta gcagcggcct ctagctccgt ctcccgggga
4801 cctgggcctg agggagggct ggagtcgcac gcgctttgtc cttagcgcct gtctgctctc
4861 ctctaactag gacccagggc ctttggcttc cccagctcat ccttggccct tccgctccac
4921 cagcctggtc tgaggcgtgc tctgtcctta gagaaggcgc ggtggccggg ttcccttccc
4981 ctagggcaca ttactaaggg ggtcaggcac tgcatgctcg ttccagcacc atctgggact
5041 gggtacagta cctccagccc cagggccctg acctgcgcac ctagcttgac atctcacgca
5101 cctcccagag ctggcgccac tgagtaatcc ggacctcacc acctcttttc ctttgagccc
5161 aaggcagagc tggagctggc gccacccaga cagcgtcagg tgtggctggg gtaggtttgg
5221 aggtctgcca gttacaccaa gtcccctctg agattcgatc aggggactgg atagattctt
5281 tcaggtactc aatcaggaag ctggaggtgt tagacaccag ccccctgcat ccttcagtag
5341 acctccctct gaacaccaca gccaggtcct gccttctggg ggcctgaata ttccagagct
5401 gatgtgatgg gctgtgcaga agggggctgt atcaacatca attagggaac caaagttgca
5461 ctatctgggc ccagattgtc tggttggcaa gagcaaagtt tccgttgatg aaacagacat
5521 cccacaacaa aaacccaagt tttctgtgct acatgtgcaa tatttgttat gaatgttatc
5581 acaagtcatt catcaagtta tctttataat cactgtagtt agatgtttca tgtccattca
5641 agtgactttt attctgagtg caatatttca atagccttgt agtgataact agtgttgctt
5701 ttgttttaga tgatctatgt gcagggcaat gcaatgaagt tgaaaaccct tgtaaatagg
5761 agaggttgca aaccaaatca agagtattta ttactattac tgctattatt attaggcctg
5821 cctttaattt tcagtgtaag tgttcagtat gccgcatcct gcctcagtat tgatcttgtg
5881 ttctttgtgc caatatgaaa aggagagggt tggttctttc ctttattgtt gaatgctccc
5941 atttaatgct ttatggcttt tactgtgtta cttttttaga ctcccgtctg cacaaaatgc
6001 aataaaaata attttattat accctt
//