LOCUS NM_003316 9021 bp mRNA linear PRI 13-MAY-2017
DEFINITION Homo sapiens tetratricopeptide repeat domain 3 (TTC3), transcript
variant 1, mRNA.
ACCESSION NM_003316
VERSION NM_003316.3
KEYWORDS RefSeq.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 9021)
AUTHORS Liu F, Wollstein A, Hysi PG, Ankra-Badu GA, Spector TD, Park D, Zhu
G, Larsson M, Duffy DL, Montgomery GW, Mackey DA, Walsh S, Lao O,
Hofman A, Rivadeneira F, Vingerling JR, Uitterlinden AG, Martin NG,
Hammond CJ and Kayser M.
TITLE Digital quantification of human eye color highlights genetic
association of three new loci
JOURNAL PLoS Genet. 6 (5), E1000934 (2010)
PUBMED 20463881
REMARK Publication Status: Online-Only
REFERENCE 2 (bases 1 to 9021)
AUTHORS Suizu F, Hiramuki Y, Okumura F, Matsuda M, Okumura AJ, Hirata N,
Narita M, Kohno T, Yokota J, Bohgaki M, Obuse C, Hatakeyama S,
Obata T and Noguchi M.
TITLE The E3 ligase TTC3 facilitates ubiquitination and degradation of
phosphorylated Akt
JOURNAL Dev. Cell 17 (6), 800-810 (2009)
PUBMED 20059950
REMARK GeneRIF: Interaction between TTC3 and Akt may contribute to the
clinical symptoms of Down syndrome.
REFERENCE 3 (bases 1 to 9021)
AUTHORS Berto G, Camera P, Fusco C, Imarisio S, Ambrogio C, Chiarle R,
Silengo L and Di Cunto F.
TITLE The Down syndrome critical region protein TTC3 inhibits neuronal
differentiation via RhoA and Citron kinase
JOURNAL J. Cell. Sci. 120 (PT 11), 1859-1867 (2007)
PUBMED 17488780
REMARK GeneRIF: These results suggest that the TTC3-RhoA-CIT-K pathway
could be a crucial determinant of in vivo neuronal development,
whose hyperactivity may result in detrimental effects on the normal
differentiation program.
REFERENCE 4 (bases 1 to 9021)
AUTHORS Rachidi M, Lopes C, Gassanova S, Sinet PM, Vekemans M, Attie T,
Delezoide AL and Delabar JM.
TITLE Regional and cellular specificity of the expression of TPRD, the
tetratricopeptide Down syndrome gene, during human embryonic
development
JOURNAL Mech. Dev. 93 (1-2), 189-193 (2000)
PUBMED 10781955
REFERENCE 5 (bases 1 to 9021)
AUTHORS Dahmane N, Ghezala GA, Gosset P, Chamoun Z, Dufresne-Zacharia MC,
Lopes C, Rabatel N, Gassanova-Maugenre S, Chettouh Z, Abramowski V,
Fayet E, Yaspo ML, Korn B, Blouin JL, Lehrach H, Poutska A,
Antonarakis SE, Sinet PM, Creau N and Delabar JM.
TITLE Transcriptional map of the 2.5-Mb CBR-ERG region of chromosome 21
involved in Down syndrome
JOURNAL Genomics 48 (1), 12-23 (1998)
PUBMED 9503011
REFERENCE 6 (bases 1 to 9021)
AUTHORS Eki T, Abe M, Naitou M, Sasanuma SI, Nohata J, Kawashima K, Ahmad
I, Hanaoka F and Murakami Y.
TITLE Cloning and characterization of novel gene, DCRR1, expressed from
Down's syndrome critical region of human chromosome 21q22.2
JOURNAL DNA Seq. 7 (3-4), 153-164 (1997)
PUBMED 9254009
REFERENCE 7 (bases 1 to 9021)
AUTHORS Tsukahara F, Hattori M, Muraki T and Sakaki Y.
TITLE Identification and cloning of a novel cDNA belonging to
tetratricopeptide repeat gene family from Down syndrome-critical
region 21q22.2
JOURNAL J. Biochem. 120 (4), 820-827 (1996)
PUBMED 8947847
REFERENCE 8 (bases 1 to 9021)
AUTHORS Ohira M, Ootsuyama A, Suzuki E, Ichikawa H, Seki N, Nagase T,
Nomura N and Ohki M.
TITLE Identification of a novel human gene containing the
tetratricopeptide repeat domain from the Down syndrome region of
chromosome 21
JOURNAL DNA Res. 3 (1), 9-16 (1996)
PUBMED 8724848
COMMENT VALIDATED REFSEQ: This record has undergone validation or
preliminary review. The reference sequence was derived from
D83077.1, D83327.1, D84296.1, BQ893819.1 and CA420000.1.
[WARNING] On Aug 1, 2017 this sequence was replaced by NM_003316.4.
On Jul 2, 2004 this sequence version replaced NM_003316.2.
Transcript Variant: This variant (1) differs in the 5' UTR and has
multiple coding region differences compared to variant 3. These
differences cause translation initiation at a downstream AUG and
result in an isoform (1) with a shorter N-terminus compared to
isoform 3. Variants 1 and 2 encode the same isoform (1).
##Evidence-Data-START##
Transcript exon combination :: D83077.1, SRR1660803.96350.1
[ECO:0000332]
RNAseq introns :: single sample supports all introns
SAMEA1965299, SAMEA1966682
[ECO:0000348]
##Evidence-Data-END##
COMPLETENESS: complete on the 3' end.
PRIMARY REFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP
1-3150 D83077.1 1-3150
3151-3914 D83327.1 1503-2266
3915-6646 D84296.1 2326-5057
6647-7246 BQ893819.1 206-805
7247-8995 D84296.1 5658-7406
8996-9021 CA420000.1 1-26 c
FEATURES Location/Qualifiers
source 1..9021
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/chromosome="21"
/map="21q22.13"
gene 1..9021
/gene="TTC3"
/gene_synonym="DCRR1; RNF105; TPRDIII"
/note="tetratricopeptide repeat domain 3"
/db_xref="GeneID:7267"
/db_xref="HGNC:HGNC:12393"
/db_xref="MIM:602259"
misc_feature 1349..1351
/gene="TTC3"
/gene_synonym="DCRR1; RNF105; TPRDIII"
/note="upstream in-frame stop codon"
CDS 1397..7474
/gene="TTC3"
/gene_synonym="DCRR1; RNF105; TPRDIII"
/EC_number="2.3.2.27"
/note="isoform 1 is encoded by transcript variant 1; TPR
repeat protein D; E3 ubiquitin-protein ligase TTC3; TPR
repeat protein 3; RING finger protein 105;
tetratricopeptide repeat protein 3; RING-type E3 ubiquitin
transferase TTC3"
/codon_start=1
/product="E3 ubiquitin-protein ligase TTC3 isoform 1"
/protein_id="NP_003307.3"
/db_xref="CCDS:CCDS13651.1"
/db_xref="GeneID:7267"
/db_xref="HGNC:HGNC:12393"
/db_xref="MIM:602259"
/translation="MDNFAEGDFTVADYALLEDCPHVDDCVFAAEFMSNDYVRVTQLY
CDGVGVQYKDYIQSERNLEFDICSIWCSKPISVLQDYCDAIKINIFWPLLFQHQNSSV
ISRLHPCVDANNSRASEINLKKLQHLELMEDIVDLAKKVANDSFLIGGLLRIGCKIEN
KILAMEEALNWIKYAGDVTILTKLGSIDNCWPMLSIFFTEYKYHITKIVMEDCNLLEE
LKTQSCMDCIEEGELMKMKGNEEFSKERFDIAIIYYTRAIEYRPENYLLYGNRALCFL
RTGQFRNALGDGKRATILKNTWPKGHYRYCDALSMLGEYDWALQANIKAQKLCKNDPE
GIKDLIQQHVKLQKQIEDLQGRTANKDPIKAFYENRAYTPRSLSAPIFTTSLNFVEKE
RDFRKINHEMANGGNQNLKVADEALKVDDCDCHPEFSPPSSQPPKHKGKQKSRNNESE
KFSSSSPLTLPADLKNILEKQFSKSSRAAHQDFANIMKMLRSLIQDGYMALLEQRCRS
AAQAFTELLNGLDPQKIKQLNLAMINYVLVVYGLAISLLGIGQPEELSEAENQFKRII
EHYPSEGLDCLAYCGIGKVYLKKNRFLEALNHFEKARTLIYRLPGVLTWPTSNVIIEE
SQPQKIKMLLEKFVEECKFPPVPDAICCYQKCHGYSKIQIYITDPDFKGFIRISCCQY
CKIEFHMNCWKKLKTTTFNDKIDKDFLQGICLTPDCEGVISKIIIFSSGGEVKCEFEH
KVIKEKVPPRPILKQKCSSLEKLRLKEDKKLKRKIQKKEAKKLAQERMEEDLRESNPP
KNEEQKETVDNVQRCQFLDDRILQCIKQYADKIKSGIQNTAMLLKELLSWKVLSTEDY
TTCFSSRNFLNEAVDYVIRHLIQENNRVKTRIFLHVLSELKEVEPKLAAWIQKLNSFG
LDATGTFFSRYGASLKLLDFSIMTFLWNEKYGHKLDSIEGKQLDYFSEPASLKEARCL
IWLLEEHRDKFPALHSALDEFFDIMDSRCTVLRKQDSGEAPFSSTKVKNKSKKKKPKD
SKPMLVGSGTTSVTSNNEIITSSEDHSNRNSDSAGPFAVPDHLRQDVEEFEALYDQHS
NEYVVRNKKLWDMNPKQKCSTLYDYFSQFLEEHGPLDMSNKMFSAEYEFFPEETRQIL
EKAGGLKPFLLGCPRFVVIDNCIALKKVASRLKKKRKKKNIKTKVEEISKAGEYVRVK
LQLNPAAREFKPDVKSKPVSDSSSAPAFENVKPKPVSANSPKPACEDVKAKPVSDNSS
RQVSEDGQPKGVSSNSPKPGSEDANYKRVSCNSPKPVLEDVKPTYWAQSHLVTGYCTY
LPFQRFDITQTPPAYINVLPGLPQYTSIYTPLASLSPEYQLPRSVPVVPSFVANDRAD
KNAAAYFEGHHLNAENVAGHQIASETQILEGSLGISVKSHCSTGDAHTVLSESNRNDE
HCGNSNNKCEVIPESTSAVTNIPHVQMVAIQVSWNIIHQEVNTEPYNPFEERQGEISR
IEKEHQVLQDQLQEVYENYEQIKLKGLEETRDLEEKLKRHLEENKISKTELDWFLQDL
EREIKKWQQEKKEIQERLKSLKKKIKKVSNASEMYTQKNDGKEKEHELHLDQSLEISN
TLTNEKMKIEEYIKKGKEDYEESHQRAVAAEVSVLENWKESEVYKLQIMESQAEAFLK
KLGLISRDPAAYPDMESDIRSWELFLSNVTKEIEKAKSQFEEQIKAIKNGSRLSELSK
VQISELSFPACNTVHPELLPESSGDDGQGLVTSASDVTGNHAALHRDPSVFSAGDSPG
EAPSALLPGPPPGQPEATQLTGPKRAGQAALSERSPVADRKQPVPPGRAARSSQSPKK
PFNSIIEHLSVVFPCYNSTELAGFIKKVRSKNKNSLSGLSIDEIVQRVTEHILDEQKK
KKPNPGKDKRTYEPSSATPVTRSSQGSPSVVVAPSPKTKGQKAEDVPVRIALGASSCE
ICHEVFKSKNVRVLKCGHKYHKGCFKQWLKGQSACPACQGRDLLTEESPSGRGWPSQN
QELPSCSSR"
misc_feature 2087..2188
/gene="TTC3"
/gene_synonym="DCRR1; RNF105; TPRDIII"
/experiment="experimental evidence, no additional details
recorded"
/note="propagated from UniProtKB/Swiss-Prot (P53804.2);
Region: TPR 1"
misc_feature 2192..2290
/gene="TTC3"
/gene_synonym="DCRR1; RNF105; TPRDIII"
/experiment="experimental evidence, no additional details
recorded"
/note="propagated from UniProtKB/Swiss-Prot (P53804.2);
Region: TPR 2"
misc_feature 2528..2530
/gene="TTC3"
/gene_synonym="DCRR1; RNF105; TPRDIII"
/experiment="experimental evidence, no additional details
recorded"
/note="Phosphoserine, by PKB/AKT2.
{ECO:0000269|PubMed:20059950}; propagated from
UniProtKB/Swiss-Prot (P53804.2); phosphorylation site"
misc_feature 3002..3112
/gene="TTC3"
/gene_synonym="DCRR1; RNF105; TPRDIII"
/experiment="experimental evidence, no additional details
recorded"
/note="propagated from UniProtKB/Swiss-Prot (P53804.2);
Region: TPR 3"
misc_feature 3122..3223
/gene="TTC3"
/gene_synonym="DCRR1; RNF105; TPRDIII"
/experiment="experimental evidence, no additional details
recorded"
/note="propagated from UniProtKB/Swiss-Prot (P53804.2);
Region: TPR 4"
misc_feature 4421..4423
/gene="TTC3"
/gene_synonym="DCRR1; RNF105; TPRDIII"
/experiment="experimental evidence, no additional details
recorded"
/note="Phosphoserine. {ECO:0000250|UniProtKB:O88196};
propagated from UniProtKB/Swiss-Prot (P53804.2);
phosphorylation site"
misc_feature 4577..4579
/gene="TTC3"
/gene_synonym="DCRR1; RNF105; TPRDIII"
/experiment="experimental evidence, no additional details
recorded"
/note="Phosphoserine. {ECO:0000250|UniProtKB:O88196};
propagated from UniProtKB/Swiss-Prot (P53804.2);
phosphorylation site"
regulatory 8983..8988
/regulatory_class="polyA_signal_sequence"
/gene="TTC3"
/gene_synonym="DCRR1; RNF105; TPRDIII"
polyA_site 9004
/gene="TTC3"
/gene_synonym="DCRR1; RNF105; TPRDIII"
/experiment="experimental evidence, no additional details
recorded"
polyA_site 9007
/gene="TTC3"
/gene_synonym="DCRR1; RNF105; TPRDIII"
ORIGIN
1 cctaaagaaa agtattaagt aaatagcagt acagatggca aatggattgc acaatatatc
61 ctctggatcc atagtgaccc tgcagagata aacctgtgat ggtcaaacaa tgtgaaaact
121 gctgtcagag acatgggcag ggtgctcttg tttacagaga agaggtgcaa aaatcaactt
181 gatggtagtg ggaagatcag gaaatgcttc ctgaaattga gtattaagaa ctaatagaca
241 ttaggtggtt gcagaataag ttttgtttag gaaggacaag cagttgggta tgactggctt
301 ctaggttgtg tgttgtggag tgactgggga taaaagcagg agcaagatca caaaaggtct
361 tctatgctta tattagggaa gttggacttt attctcaagc tgaagggaag ctgttgcatg
421 gttttaagca gtaaagtgat atgatcagag ttttagagga tgccaagatt gaaggcaagt
481 ctgaccagtt aggagactgc ttgttaaatt agttcagagg agaaacagtg aaggcagtgg
541 cactgggcat gaagaagtat atgtgtgcta attttagatt tcttagggaa gcagaaatga
601 caagagttag tggtccattg gacagaaata ttgaaggaga ctggggagtc taggttgact
661 cccagggttt aggtttgggc agtaaaatga catgtagaac aattaactga taaacagcat
721 acagaaagag gaaagaactt catttcatat gtttgttttt gaggaaaaga tgtttgtttt
781 tgaacttcct gattcagagg ggcttgtggg acatcttggt taagatcctg tagtagttct
841 agtagggtct agaagtcaag agatacaacc ccgcctggaa ggatttggga gtcttcagca
901 tttggaattt tggaagccat tgtttactgc agtgcatatg agataattta aactggtaca
961 tagataaaca cttgaaaaaa ttttaataga taggaattta atgtgtatga gaaacataac
1021 ttgcacatct aacctttgat aatcatggac attatcactt aggccaagtg agctcaataa
1081 aagggaaata ttaactaaaa atatatgtga tacatggaaa tggcaaaaat cacggtgaag
1141 ctatgcaaac gatgcaagtt cagaaagtgc tgtgttaagt catgggtgtg gtggataaat
1201 tcacccaagg agagagtaag agtgagaaga aaagagagtt gacattgggt ggcggggaat
1261 ggagaagaag aacccatgaa ggaaactgag gaagagcagc cagacgaata ggaggaaaac
1321 caggagaaga tggtctttgg agtccaaatg aagagttctg aggaggaagt ggtccacagc
1381 gtcaaacttg tgcaccatgg acaattttgc tgagggagat ttcactgtgg cggattatgc
1441 cttgttagaa gattgccctc acgtggatga ttgtgtcttt gctgctgaat ttatgagcaa
1501 tgattatgtt cgtgtgactc agctttactg tgatggggtg ggtgtgcaat ataaagatta
1561 tatccaaagt gagaggaatt tggaatttga catctgcagt atatggtgta gtaaaccaat
1621 ttctgtcctg caagattatt gcgatgccat taaaataaac atcttctggc cacttctgtt
1681 tcaacatcaa aacagttccg taatatcacg attgcatccc tgtgtggacg ccaacaattc
1741 acgtgcttct gagataaatt tgaagaaact acaacatctt gagttgatgg aagatattgt
1801 ggatttggca aagaaagttg ctaatgattc attccttatt ggaggcttat tgagaattgg
1861 ttgtaaaata gaaaataaaa tcttggcaat ggaagaagct ctgaattgga taaaatatgc
1921 aggcgatgta acaattctaa ctaaattagg atcaattgac aattgttggc ctatgttaag
1981 tattttcttt actgaataca agtaccacat aactaaaatt gtaatggaag actgcaattt
2041 gcttgaagaa cttaaaactc aaagttgtat ggattgtata gaggaaggag aactaatgaa
2101 aatgaaagga aatgaagagt tttccaaaga aagatttgat atagctatta tctattacac
2161 cagagccatt gaatatagac ctgaaaacta ccttctttat ggtaaccgag ctctttgttt
2221 tcttcgtact ggacagttta gaaatgcact cggtgatgga aagagagcca ctattctgaa
2281 gaacacttgg ccaaagggtc attatcgtta ttgtgatgct ctttctatgc tgggggaata
2341 tgactgggcc ctgcaagcaa acataaaagc tcaaaaactc tgtaaaaatg accctgaggg
2401 aatcaaggat ctaattcagc agcatgtaaa gttacaaaaa caaatagaag acctacaagg
2461 tcgaacagca aataaggatc caattaaagc cttttatgaa aacagggcct acacacctag
2521 gagtttatca gcacctatat ttactacttc acttaacttt gtggagaagg aaagagattt
2581 cagaaaaatt aatcacgaaa tggccaacgg tggtaatcag aatctaaagg tggcggatga
2641 ggcgttgaag gtagatgatt gtgactgtca tcctgaattt tcaccaccat caagtcagcc
2701 tccaaaacat aaaggaaaac aaaaatctcg aaacaatgaa tcagaaaagt tcagttctag
2761 ttcaccattg actttaccag cagatttgaa gaacatcttg gagaaacagt tttctaaatc
2821 ttccagagct gcacaccagg attttgctaa tataatgaaa atgctgagaa gcttaattca
2881 agatggctat atggccttat tggagcagcg ttgccgcagc gctgcacagg cctttacaga
2941 gttgctgaac ggtttagatc ctcaaaaaat aaagcaattg aacctggcca tgattaacta
3001 tgttttggtc gtctatggac ttgccatttc tctccttgga ataggacagc ctgaggaatt
3061 atctgaagcc gaaaaccagt ttaagaggat tattgaacac taccccagtg agggccttga
3121 ttgcttggcc tactgtggaa ttggaaaagt atatttgaaa aaaaacagat ttctagaagc
3181 tctcaatcac tttgagaaag caagaacctt gatttatcgt cttcctggag tgttaacttg
3241 gcccacgagt aatgtgatta ttgaagagtc tcagccacaa aaaataaaga tgctgttaga
3301 gaaatttgtt gaagaatgca agttccctcc agtgccagat gccatttgtt gctatcagaa
3361 gtgccatgga tattctaaga tccagatata cataactgat ccagacttta agggttttat
3421 acgcatcagc tgttgccagt actgtaaaat agaatttcac atgaattgct ggaagaagtt
3481 aaaaactaca acctttaatg ataaaattga caaggatttt ctacaaggaa tatgtcttac
3541 ccctgactgt gaaggtgtca tttctaagat tatcatcttc agcagtggtg gtgaagttaa
3601 atgtgaattt gaacacaagg tcataaaaga aaaggttcct ccaagaccta ttctgaaaca
3661 gaaatgttct agcctagaga aactaagact gaaagaagac aaaaaattga agagaaagat
3721 ccaaaaaaaa gaagcaaaaa agttagcaca agaaagaatg gaggaggact taagagaaag
3781 taatccaccc aaaaatgaag agcagaaaga aactgtagac aatgttcagc gttgtcagtt
3841 ccttgatgac agaattctac agtgtataaa gcagtatgct gacaagatta aatccggcat
3901 acagaataca gccatgcttc tcaaagaatt gctttcttgg aaagttttga gcacagaaga
3961 ctatacaacc tgtttttcta gcagaaattt tctaaatgaa gcagtggact atgttattcg
4021 ccacttgatt caagaaaata acagagtaaa gacaagaata tttctgcatg ttttgagtga
4081 gcttaaagaa gtggagccca aattagccgc ctggatccaa aaacttaata gctttggctt
4141 agatgccaca ggaactttct tttctcgtta tggagcatct cttaaactgc ttgattttag
4201 tatcatgact ttcctctgga atgagaaata tggtcacaaa ctagactcta tagaaggaaa
4261 gcaacttgat tatttctctg agccagcatc attgaaggaa gcccgttgtt taatatggct
4321 gctagaagaa cacagagaca agttcccagc attgcatagt gctttagatg aattctttga
4381 tataatggac agccgctgta ctgtgttaag gaaacaagat agtggtgaag caccgtttag
4441 ttcaaccaag gtgaaaaaca aaagcaagaa aaagaagcca aaggattcaa agcctatgtt
4501 agttgggtct ggaacaactt cagtaacttc aaataatgag atcatcactt caagtgaaga
4561 ccatagcaat cgaaattcag attctgcagg cccatttgca gtgcctgacc atcttcggca
4621 agatgtagaa gaattcgaag ctctctatga ccaacacagt aacgaatatg ttgtccgcaa
4681 taagaagcta tgggacatga acccaaaaca aaaatgttca actctatatg attacttctc
4741 tcagtttttg gaggaacatg gtcccttgga catgagtaac aagatgttct ctgcagaata
4801 tgagtttttc ccagaagaaa ctcgacagat actagaaaaa gcaggaggtt taaaaccttt
4861 tctcttggga tgccctcgtt ttgttgtgat tgacaactgt attgcactga agaaggttgc
4921 atcacggctc aagaaaaaaa ggaagaagaa aaacattaaa acaaaagtag aagaaatttc
4981 aaaagcaggg gagtatgtac gagttaaact acaactgaat ccagctgcta gggaatttaa
5041 accagatgta aagtctaaac cagtgtcaga ttcatcttca gcaccagctt ttgaaaatgt
5101 gaaacccaaa cctgtgtctg caaattctcc caagccagct tgtgaagatg tgaaggccaa
5161 accagtatcc gacaattctt ctagacaagt ttctgaggat gggcaaccca aaggggtctc
5221 ttctaattct cctaaaccag gctctgagga tgcaaattac aagcgagtct cctgtaattc
5281 ccccaaaccg gttcttgagg atgtgaaacc aacttattgg gctcaatccc atttggtcac
5341 aggatactgt acgtatcttc ctttccagag atttgatatc acccagacac cgccagcata
5401 cataaacgtg ttaccaggtt tgccccagta caccagcata tatacaccct tggccagcct
5461 ttctcctgaa tatcagctac caagatcagt accagtggtg ccgtcttttg tagccaatga
5521 cagagcagat aaaaatgctg ctgcctattt tgagggtcat catttgaatg ctgagaatgt
5581 tgctggtcac cagattgcct ctgaaacaca gatccttgag ggctctttgg gaatatctgt
5641 aaagtcacac tgcagcacag gtgatgctca tacagtcctg agtgagtcta acagaaatga
5701 tgagcactgt ggaaattcta acaacaaatg tgaagtaatt ccagaaagca ccagtgcagt
5761 aacaaacatt ccacacgtgc agatggttgc catacaggta tcttggaaca taatacacca
5821 agaagtcaat actgagccat ataatccttt tgaggaacga caaggggaaa tttcacggat
5881 tgaaaaggag caccaagtat tacaagacca acttcaagaa gtgtatgaaa attatgagca
5941 gataaaactt aagggcttag aagagaccag ggacctggaa gagaagttga aaaggcactt
6001 agaagaaaac aagatctcaa agacggaatt agattggttc cttcaagatt tggaaagaga
6061 aattaaaaaa tggcaacagg aaaaaaaaga aatccaagaa agactaaaat cactgaagaa
6121 gaaaattaaa aaggtttcaa atgccagtga aatgtatacc cagaaaaatg atggaaagga
6181 aaaggaacat gaattacatc tggatcagtc ccttgaaatc agcaacacac ttacaaatga
6241 gaaaatgaaa atagaagagt atataaagaa agggaaagag gattatgaag agagtcatca
6301 gagagctgtg gctgcagagg tatccgtact tgaaaactgg aaggagagtg aagtgtataa
6361 gctacagatc atggagtcac aagcagaagc ctttctgaag aagctggggc tgattagccg
6421 tgatcctgca gcatatcctg acatggagtc tgatatacgt tcatgggaat tgtttctttc
6481 taatgttaca aaagaaattg agaaagcaaa gtctcagttt gaagaacaaa ttaaggcaat
6541 taaaaatggt tctcggctca gtgaactttc taaagtgcag atttctgagc tttcatttcc
6601 tgcctgtaac acggttcatc ccgagttact ccctgagtct tcaggcgacg atggccaagg
6661 gcttgtgact tctgcaagcg acgtgactgg aaaccacgca gcacttcaca gggatcctag
6721 tgtgttctct gctggtgatt ccccagggga ggctccttct gcgctgttgc cagggccacc
6781 ccctggtcag cctgaagcca ctcagctgac agggccaaaa cgggctggcc aggcagctct
6841 gtcagaacga agccctgtgg ctgatcggaa gcagcctgtt cctccaggac gtgctgcgcg
6901 ttcaagccag tctccaaaaa agccgttcaa tagtattatt gagcacctgt cagtggtatt
6961 cccatgttac aacagcactg agcttgctgg ttttattaaa aaagtgcgaa gcaaaaacaa
7021 gaactcactc tcaggattga gtattgatga aattgtccaa agagtgacag aacacattct
7081 agatgaacag aaaaagaaaa agccaaaccc aggaaaggac aagaggactt atgagcccag
7141 ctctgccacc cccgtgacca ggtcctccca gggctcaccc tcggtggttg ttgcaccatc
7201 acccaaaacc aaggggcaga aagcagaaga tgtccctgtg aggattgcac tgggtgcaag
7261 ttcctgtgaa atatgccacg aggtgttcaa atcaaaaaac gtgcgtgtgc tcaaatgtgg
7321 gcacaagtat cacaaagggt gctttaagca gtggcttaaa gggcagagcg cttgcccggc
7381 ctgccagggt cgtgatctcc tgacagaaga gtcaccttct ggaagaggct ggcccagtca
7441 gaatcaggag ctgccttcct gctcttctag gtagtcacac ttcactaaag tgtcatccac
7501 cagtgtgttg aatccgaaga atgacaattt tctaccactg gtgtaaaaaa caaacatttg
7561 aagacccttg tgcattgtgt gtcacaaagc taaatacatg gaaatcgtta atatcgctga
7621 tattaagtaa tttccccact ctgagtgaat actttgatga ttgccaacag tggctaataa
7681 aatgacggct accacactca tgggtcactg gggctgcgca gggctctttg aggtgggtgg
7741 cttcttttgg aaagtactat gaacgtctcg aagcagtatt ctagtgataa gaattcttaa
7801 catagccaag cgccccacgt ttgttcccca cgtttgttcc ccttttctgt ttgaaaaacc
7861 tgttctggta gctccacaag agagatgata ctgacttttt aaatttttta caagagtctg
7921 tattcctgat atgcctatat ttttcctcaa agattctgca ttttaaggat gggcataagc
7981 aaactatatt ttaataattt atagttaatg ttaaaatatt ggctgattta gaccaaaaga
8041 ttcaaatctc ctctttgtga aatcccatct gcatttgatt ttttattatt ttatgttccc
8101 ccgttagatt gttttaagtg tttgcttttc atcttttata gatgtaatct gattttcaaa
8161 aatcattaac actttttaat tagtatcgac taagactttt tccccctgga atcgaggctg
8221 tgtgtccgtc atcccagccc ccggttggag cctgctcttt gaactccgct gccttcctta
8281 gcagcttctg tcctcttctg tgagtcagtc agcgagtgct tgggatccgc atccagccgt
8341 gctgagcaca caacaggctg tgtgtggaaa tggccaccac cattctcctt ccccacccca
8401 ccacaaaaag agaagctgtg tctttagaca accctgaggt atctgtgtta caatcgttct
8461 gtgtttgata tttgtgtaaa gtatgcatgc agtcttgtac tgtgacctaa gaacaaaact
8521 gtaactgcat tagaaaccat gaaaaaatta gatattgttt tgtgactttt agacagtggt
8581 aaatatagaa ccatgaattc tggtcacatt ccatttctct ccaacatgaa ggatcaaaaa
8641 atgtttttca atgtgttctt tgttccactg gaaacttaga gtcatgagtt tatgagctga
8701 tttggtcacc ttcctctgcc tttgttcact gtgagttctg atgtcttagt gacttagttc
8761 ttagaagctc acgccttagt ttgaaacaga ttctccacgg tggtccccaa aacactgtct
8821 gcatatccat aagaattgag cgctatgggt gttaacgtgc atgaggatca gtttgcagca
8881 gcaagtacaa aaggagaaga ggaacatccg ttgaatgagt gtgttttgta cataacttca
8941 gatacttgtg aacatgcctt atatttgtcc aacaactgtc agaataaaga acattctaaa
9001 atgagaaaaa aaaaaaaaaa a
//