LOCUS NM_001400784 4781 bp mRNA linear PRI 13-DEC-2024
DEFINITION Homo sapiens ubiquitin specific peptidase 28 (USP28), transcript
variant 23, mRNA.
ACCESSION NM_001400784
VERSION NM_001400784.1
KEYWORDS RefSeq.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4781)
AUTHORS Jin,C., Einig,E., Xu,W., Kollampally,R.B., Schlosser,A., Flentje,M.
and Popov,N.
TITLE The dimeric deubiquitinase USP28 integrates 53BP1 and MYC functions
to limit DNA damage
JOURNAL Nucleic Acids Res 52 (6), 3011-3030 (2024)
PUBMED 38227944
REMARK GeneRIF: The dimeric deubiquitinase USP28 integrates 53BP1 and MYC
functions to limit DNA damage.
REFERENCE 2 (bases 1 to 4781)
AUTHORS Meitinger,F., Belal,H., Davis,R.L., Martinez,M.B., Shiau,A.K.,
Oegema,K. and Desai,A.
TITLE Control of cell proliferation by memories of mitosis
JOURNAL Science 383 (6690), 1441-1448 (2024)
PUBMED 38547292
REMARK GeneRIF: Control of cell proliferation by memories of mitosis.
REFERENCE 3 (bases 1 to 4781)
AUTHORS Karapurkar,J.K., Colaco,J.C., Suresh,B., Tyagi,A., Woo,S.H.,
Jo,W.J., Ko,N., Singh,V., Hong,S.H., Oh,S.J., Kim,K.S. and
Ramakrishna,S.
TITLE USP28 promotes tumorigenesis and cisplatin resistance by
deubiquitinating MAST1 protein in cancer cells
JOURNAL Cell Mol Life Sci 81 (1), 145 (2024)
PUBMED 38498222
REMARK GeneRIF: USP28 promotes tumorigenesis and cisplatin resistance by
deubiquitinating MAST1 protein in cancer cells.
Publication Status: Online-Only
REFERENCE 4 (bases 1 to 4781)
AUTHORS Zhou,W., Chen,J. and Wang,J.
TITLE Comprehensive prognostic and immunological analysis of Ubiquitin
Specific Peptidase 28 in pan-cancers and identification of its role
in hepatocellular carcinoma cell lines
JOURNAL Aging (Albany NY) 15 (13), 6545-6576 (2023)
PUBMED 37450415
REMARK GeneRIF: Comprehensive prognostic and immunological analysis of
Ubiquitin Specific Peptidase 28 in pan-cancers and identification
of its role in hepatocellular carcinoma cell lines.
REFERENCE 5 (bases 1 to 4781)
AUTHORS Li,J., Peng,J., Wu,L., Shen,X., Zhen,X., Zhang,Y., Ma,H., Xu,Y.,
Xiong,Q., Zhu,Q. and Zhang,P.
TITLE The deubiquitinase USP28 maintains the expression of the
transcription factor MYCN and is essential in neuroblastoma cells
JOURNAL J Biol Chem 299 (7), 104856 (2023)
PUBMED 37230388
REMARK GeneRIF: The deubiquitinase USP28 maintains the expression of the
transcription factor MYCN and is essential in neuroblastoma cells.
REFERENCE 6 (bases 1 to 4781)
AUTHORS Popov,N., Herold,S., Llamazares,M., Schulein,C. and Eilers,M.
TITLE Fbw7 and Usp28 regulate myc protein stability in response to DNA
damage
JOURNAL Cell Cycle 6 (19), 2327-2331 (2007)
PUBMED 17873522
REMARK GeneRIF: Usp28 dissociates from Fbw7alpha in response to UV
irradiation, providing a mechanism how Fbw7-mediated degradation of
Myc is enhanced upon DNA damage.
REFERENCE 7 (bases 1 to 4781)
AUTHORS Popov,N., Wanzel,M., Madiredjo,M., Zhang,D., Beijersbergen,R.,
Bernards,R., Moll,R., Elledge,S.J. and Eilers,M.
TITLE The ubiquitin-specific protease USP28 is required for MYC stability
JOURNAL Nat Cell Biol 9 (7), 765-774 (2007)
PUBMED 17558397
REMARK GeneRIF: High expression levels of USP28 are found in colon and
breast carcinomas, and stabilization of MYC by USP28 is essential
for tumour-cell proliferation.
REFERENCE 8 (bases 1 to 4781)
AUTHORS Zhang,D., Zaugg,K., Mak,T.W. and Elledge,S.J.
TITLE A role for the deubiquitinating enzyme USP28 in control of the
DNA-damage response
JOURNAL Cell 126 (3), 529-542 (2006)
PUBMED 16901786
REMARK GeneRIF: Using a human cell line that faithfully recapitulated the
Chk2-p53-PUMA pathway, we show that USP28 is required to stabilize
Chk2 and 53BP1 in response to DNA damage.
REFERENCE 9 (bases 1 to 4781)
AUTHORS Puente,X.S., Sanchez,L.M., Overall,C.M. and Lopez-Otin,C.
TITLE Human and mouse proteases: a comparative genomic approach
JOURNAL Nat Rev Genet 4 (7), 544-558 (2003)
PUBMED 12838346
REMARK Review article
REFERENCE 10 (bases 1 to 4781)
AUTHORS Valero,R., Bayes,M., Francisca Sanchez-Font,M., Gonzalez-Angulo,O.,
Gonzalez-Duarte,R. and Marfany,G.
TITLE Characterization of alternatively spliced products and
tissue-specific isoforms of USP28 and USP25
JOURNAL Genome Biol 2 (10), RESEARCH0043 (2001)
PUBMED 11597335
REMARK GeneRIF: molecular cloning of USP28 & characterization of
alternatively spliced products and tissue-specific isoforms
COMMENT REVIEWED REFSEQ: This record has been curated by NCBI staff. The
reference sequence was derived from AP001874.4, AP003170.4 and
KF459544.1.
Summary: The protein encoded by this gene is a deubiquitinase
involved in the DNA damage pathway and DNA damage-induced
apoptosis. Overexpression of this gene is seen in several cancers.
[provided by RefSeq, Oct 2016].
Sequence Note: The RefSeq transcript and protein were derived from
genomic sequence to make the sequence consistent with the reference
genome assembly. The genomic coordinates used for the transcript
record were based on transcript alignments.
Publication Note: This RefSeq record includes a subset of the
publications that are available for this gene. Please see the Gene
record to access additional publications.
##Evidence-Data-START##
RNAseq introns :: mixed sample support SAMEA1965299, SAMEA1966682
[ECO:0006172]
##Evidence-Data-END##
PRIMARY REFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP
1-128 AP001874.4 7291-7418 c
129-261 AP003170.4 108788-108920 c
262-367 AP003170.4 97950-98055 c
368-527 AP003170.4 96885-97044 c
528-614 AP003170.4 90536-90622 c
615-752 AP003170.4 89707-89844 c
753-826 AP003170.4 88207-88280 c
827-906 AP003170.4 87154-87233 c
907-1055 AP003170.4 85484-85632 c
1056-1183 AP003170.4 83520-83647 c
1184-1279 AP003170.4 79892-79987 c
1280-1459 AP003170.4 73945-74124 c
1460-1668 AP003170.4 71461-71669 c
1669-1739 AP003170.4 70172-70242 c
1740-1968 AP003170.4 68563-68791 c
1969-2160 AP003170.4 65350-65541 c
2161-2300 AP003170.4 64585-64724 c
2301-2486 AP003170.4 64238-64423 c
2487-2582 AP003170.4 62776-62871 c
2583-2761 AP003170.4 61155-61333 c
2762-2840 AP003170.4 60960-61038 c
2841-2920 AP003170.4 60085-60164 c
2921-3044 AP003170.4 59445-59568 c
3045-3240 AP003170.4 57770-57965 c
3241-3524 KF459544.1 112-395
3525-4781 AP003170.4 54162-55418 c
FEATURES Location/Qualifiers
source 1..4781
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/chromosome="11"
/map="11q23.2"
gene 1..4781
/gene="USP28"
/note="ubiquitin specific peptidase 28"
/db_xref="GeneID:57646"
/db_xref="HGNC:HGNC:12625"
/db_xref="MIM:610748"
exon 1..128
/gene="USP28"
/inference="alignment:Splign:2.1.0"
CDS 72..3416
/gene="USP28"
/EC_number="3.4.19.12"
/note="isoform 20 is encoded by transcript variant 23;
ubiquitin thioesterase 28; deubiquitinating enzyme 28;
ubiquitin-specific-processing protease 28; ubiquitin
thiolesterase 28"
/codon_start=1
/product="ubiquitin carboxyl-terminal hydrolase 28 isoform
20"
/protein_id="NP_001387713.1"
/db_xref="GeneID:57646"
/db_xref="HGNC:HGNC:12625"
/db_xref="MIM:610748"
/translation="MTAELQQDDAAGAADGHGSASNGDITQAVSLLTDERVKEPSQDT
VATEPSEVEGSAANKEVLAKVIDLTHDNKDDLQAAIALSLLESPKIQADGRDLNRMHE
ATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEF
RRLVLSYSLPQNVLENCRSHTEKRNIMFMQELQYLFALMMGSNRKFVDPSAALDLLKG
AFRSSEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSSPRNKSENPMVQLFYGTFLTEGV
REGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDVELLPSDHSVKYGQERWFTKL
PPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYRSKELIRNKRECIRKLKE
EIKILQQKLERYVKYGSGPARFPLPDMLKYVIEFASTKPASESCPPESDTHMTLPLSS
VHCSVSDQTSKESTSTESSSQDVESTFSSPEDSLPKSKPLTSSRSSMEMPSQPAPRTV
TDEEINFVKTCLQRWRSEIEQDIQDLKTCIASTTQTIEQMYCDPLLRQVPYRLHAVLV
HEGQANAGHYWAYIYNQPRQSWLKYNDISVTESSWEEVERDSYGGLRNVSAYCLMYIN
DKLPYFNAEAAPTESDQMSEVEALSVELKHYIQEDNWRFEQEVEEWEEEQSCKIPQME
SSTNSSSQDYSTSQEPSVASSHGVRCLSSEHAVIVKEQTAQAIANTARAYEKSGVEAA
LSELKEAEPKKPMPQETNLAEQSEQPPKANDAESTAQPNSEVSEVEIPSVGRILVRSD
ADGYDEEVMLSPAMQGVILAIAKARQTFDRDGSEAGLIKAFHEEYSRLYQLAKETPTS
HSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADKNLSYDERSISIMKVAQAKLKEIGP
DDMNMEEYKKWHEDYSLFRKVSVYLLTGLELYQKGKYQEALSYLVYAYQSNAALLMKG
PRRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINN
DISKDDLDAIEVMRNHWCSYLGQDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRP
NSPYDLCSRFAAVMESIQGVSTVTVK"
exon 129..261
/gene="USP28"
/inference="alignment:Splign:2.1.0"
exon 262..367
/gene="USP28"
/inference="alignment:Splign:2.1.0"
exon 368..527
/gene="USP28"
/inference="alignment:Splign:2.1.0"
exon 528..614
/gene="USP28"
/inference="alignment:Splign:2.1.0"
exon 615..752
/gene="USP28"
/inference="alignment:Splign:2.1.0"
exon 753..826
/gene="USP28"
/inference="alignment:Splign:2.1.0"
exon 827..906
/gene="USP28"
/inference="alignment:Splign:2.1.0"
exon 907..1055
/gene="USP28"
/inference="alignment:Splign:2.1.0"
exon 1056..1183
/gene="USP28"
/inference="alignment:Splign:2.1.0"
exon 1184..1279
/gene="USP28"
/inference="alignment:Splign:2.1.0"
exon 1280..1459
/gene="USP28"
/inference="alignment:Splign:2.1.0"
exon 1460..1668
/gene="USP28"
/inference="alignment:Splign:2.1.0"
exon 1669..1739
/gene="USP28"
/inference="alignment:Splign:2.1.0"
exon 1740..1968
/gene="USP28"
/inference="alignment:Splign:2.1.0"
exon 1969..2160
/gene="USP28"
/inference="alignment:Splign:2.1.0"
exon 2161..2300
/gene="USP28"
/inference="alignment:Splign:2.1.0"
exon 2301..2486
/gene="USP28"
/inference="alignment:Splign:2.1.0"
exon 2487..2582
/gene="USP28"
/inference="alignment:Splign:2.1.0"
exon 2583..2761
/gene="USP28"
/inference="alignment:Splign:2.1.0"
exon 2762..2840
/gene="USP28"
/inference="alignment:Splign:2.1.0"
exon 2841..2920
/gene="USP28"
/inference="alignment:Splign:2.1.0"
exon 2921..3044
/gene="USP28"
/inference="alignment:Splign:2.1.0"
exon 3045..3240
/gene="USP28"
/inference="alignment:Splign:2.1.0"
exon 3241..4781
/gene="USP28"
/inference="alignment:Splign:2.1.0"
regulatory 4763..4768
/regulatory_class="polyA_signal_sequence"
/gene="USP28"
/note="hexamer: AATAAA"
polyA_site 4781
/gene="USP28"
/note="major polyA site"
ORIGIN
1 ctagtcctga gaggctgggc cggcggcggc tgcggcggga gaccggtgac ccgcggctgg
61 gcgcctcggc catgactgcg gagctgcagc aggacgacgc ggccggcgcg gcagacggcc
121 acggctcggc cagtaatggt gacattactc aggcagtcag ccttctcact gatgagagag
181 ttaaggagcc cagtcaagac actgttgcta cagaaccatc tgaagtagag gggagtgctg
241 ccaacaagga agtattagca aaagttatag accttactca tgataacaaa gatgatcttc
301 aggctgccat tgctttgagt ctactggagt ctcccaaaat tcaagctgat ggaagagatc
361 ttaacaggat gcatgaagca acctctgcag aaactaaacg ctcaaagaga aaacgctgtg
421 aagtctgggg agaaaacccc aatcccaatg actggaggag agttgatggt tggccagttg
481 ggctgaaaaa tgttggcaat acatgttggt ttagtgctgt tattcagtct ctctttcaat
541 tgcctgaatt tcgaagactt gttctcagtt atagtctgcc acaaaatgta cttgaaaatt
601 gtcgaagtca tacagaaaag agaaatatca tgtttatgca agagcttcag tatttgtttg
661 ctctaatgat gggatcaaat agaaaatttg tagacccgtc tgcagccctg gatctattaa
721 agggagcatt ccgatcatct gaggaacagc agcaagatgt gagtgaattc acacacaagc
781 tcctggattg gctagaggac gcattccagc tagctgttaa tgttaacagc agtcccagga
841 acaaatctga aaatccaatg gtgcagctgt tctatggtac tttcctgact gaaggggttc
901 gtgaaggaaa acccttttgt aacaatgaga ccttcggcca gtatcctctt caggtaaacg
961 gttatcgcaa cttagacgag tgtttggaag gggccatggt ggagggtgat gttgagcttc
1021 ttccctccga tcactcggtg aagtatggac aagagcgttg gtttacaaag ctacctccag
1081 tgttgacctt tgaactctca agatttgagt ttaatcagtc ccttgggcag ccagagaaaa
1141 ttcacaataa gctggaattt cctcagatta tttatatgga caggtacatg tacaggagca
1201 aggagcttat tcgaaataag agagagtgta ttcgaaagtt gaaggaggaa ataaaaattc
1261 tgcagcaaaa attggaaagg tatgtgaaat atggctcagg cccagctcgg ttcccgctcc
1321 cggacatgct gaaatatgtt attgaatttg ctagtacaaa acctgcctca gaaagctgtc
1381 cacctgaaag tgacacacat atgacattac cactttcttc agtgcactgc tcggtttctg
1441 accagacatc caaggaaagt acaagtacag aaagctcttc tcaggatgtt gaaagtacct
1501 tttcttctcc tgaagattct ttacccaagt ctaaaccact gacatcttct cggtcttcca
1561 tggaaatgcc ttcacagcca gctccacgaa cagtcacaga tgaggagata aattttgtta
1621 agacctgtct tcagagatgg aggagtgaga ttgaacaaga tatacaagat ttaaagactt
1681 gtattgcaag tactactcag actattgaac agatgtactg cgatcctctc cttcgtcagg
1741 tgccttatcg cttgcatgca gttcttgttc atgaaggaca agcaaatgct ggacactatt
1801 gggcctatat ctataatcaa ccccgacaga gctggctcaa gtacaatgac atctctgtta
1861 ctgaatcttc ctgggaagaa gttgaaagag attcctatgg aggcctgaga aatgttagtg
1921 cttactgtct gatgtacatt aatgacaaac taccctactt caatgcagag gcagccccaa
1981 ctgaatcaga tcaaatgtca gaagtggaag ccctatctgt ggaactcaag cattacattc
2041 aggaggataa ctggcggttt gagcaggaag tagaggagtg ggaagaagag cagtcttgca
2101 aaatccctca aatggagtcc tccaccaact cctcatcaca ggactactct acatcacaag
2161 agccttcagt agcctcttct catggggttc gctgcttgtc gtctgagcat gctgtgattg
2221 taaaggagca aactgcccag gctattgcaa acacagcccg tgcctatgag aagagcggtg
2281 tagaagcggc actgagtgag cttaaagaag ctgaacccaa gaagcccatg ccccaggaaa
2341 caaaccttgc agagcagtca gaacagcccc caaaggctaa tgatgcagag tctactgccc
2401 agcctaattc tgaggtctct gaagtcgaga ttcccagtgt gggaaggatt ctggttagat
2461 ctgatgcaga tggatatgat gaggaggtga tgctgagccc tgccatgcaa ggggtcatcc
2521 tggccatagc taaagcccgt cagacctttg accgagatgg gtctgaagca gggctgatta
2581 aggcattcca tgaagaatac tccaggctct atcagcttgc caaagagacc cccacctctc
2641 acagtgatcc tcgacttcag catgtccttg tctacttttt ccaaaatgaa gcacccaaaa
2701 gggtagtaga acgaaccctt ctggaacagt ttgcagataa aaatcttagc tatgatgaaa
2761 gatcaatcag cattatgaag gtggctcaag cgaaactgaa ggaaattggt ccagatgaca
2821 tgaatatgga agagtacaag aagtggcatg aagattatag tttgttccga aaagtgtctg
2881 tgtatctcct aacaggccta gaactctatc aaaaaggaaa gtaccaagag gcactttcct
2941 acctggtata tgcctaccag agcaatgctg ccctgctgat gaaggggccc cgccgggggg
3001 tcaaagaatc cgtgattgct ttataccgaa gaaaatgcct tctggagctg aatgccaaag
3061 cagcttctct ttttgaaaca aatgatgatc actccgtaac tgagggcatt aatgtgatga
3121 atgaactgat catcccctgc attcacctta tcattaataa tgacatttcc aaggatgatc
3181 tggatgccat tgaggtcatg agaaaccatt ggtgctctta ccttgggcaa gatattgcag
3241 aaaatctgca gctgtgccta ggggagtttc tacccagact tctagatcct tctgcagaaa
3301 tcatcgtctt gaaagagcct ccaactattc gacccaattc tccctatgac ctatgtagcc
3361 gatttgcagc tgtcatggag tcaattcagg gagtttcaac tgtgacagtg aaataagctc
3421 ccacatgttc aaggcccatt ctggttcctg gctgcctgcc tcttgcacag aagttcgttg
3481 tcatagtgct caccttggga aaaggattag gtgggcacat aagattccga tcagacccca
3541 accatgctgc atgtgtaaag aaggattgaa aataaaattg cactttttag gtacaaaatc
3601 ataaaagctg tttcactaga aaaggcagaa agcagtgtat taaggtgttg aattacgcca
3661 gaagacctga aatgccttgt acctacaaca atgcttaggc ttttctaagc ctcttgccac
3721 ttttaaaatt atccttcagg cataaatatt tttgacagca gaatagaaga atgattcatg
3781 agaacctgaa ccagatgaac agctactagt tattttatca aatacagatg acatttaaaa
3841 attcttaact acaagagatt agaaatataa accttgcctg gctcttgcca ggagataaca
3901 aaatgggttg ctgatgaact gcaccctttt acatgtgggt agaatataag ctcacatggc
3961 agtgagatgt tgaaaagtca aaagagacct gtctctctcc tttcttttct atctttaaac
4021 cagaaaacct catactcagt cctcagtgaa agaaagtaaa gtattaagga ctttagacag
4081 aagagcattg tgtaacttga ctgaagatca tccattaata gttattaggc atttaggtaa
4141 aattttctaa tacctaaaaa ttgtcaaaaa cagtcaatag ggctactgct ggcccaaaga
4201 ccatttaggt ccacctcctc ttttttgctc tttttttttt tctgtgacag tttcactgtg
4261 tcgcccaggc tggcgttcag tggtgcaatc tcagctcact gcaaactctg tctcctgggc
4321 tcaagtgatt ctcgtgcctc agcctcccga atagctggaa ttacgggcat gcaccaccac
4381 acctggctaa tttttgtatt tttaatagag atggggtttc accatattgg ccaggctgat
4441 ctctaactcc tggcctcaag tgatctatct gcctccctca gcctcccaaa gtctgggatt
4501 gcagacaagt catcgtaccc ggccttcttt tttgccctta aaagtaaggg atgtgggttt
4561 gtacaaaaaa aaaaaaaaaa aaaaaaaaac cagcatacat atgcaaaact atatatatat
4621 gtatatgtag agaaaaatac ttcccattga tcatttttaa aaggcttctg attggatatt
4681 gtgttttaac caaattttaa agattaatgg aatcatgaaa gggaaaaaaa ttgatacaac
4741 tatgcagatt ttataaatgt gcaataaaag tatttgtttt a
//