LOCUS NM_001372095 10275 bp mRNA linear PRI 09-JUN-2024
DEFINITION Homo sapiens ubiquitin specific peptidase 47 (USP47), transcript
variant 8, mRNA.
ACCESSION NM_001372095
VERSION NM_001372095.1
KEYWORDS RefSeq.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 10275)
AUTHORS Lei,H., Xu,H., Yang,L., Wang,Y., Zhang,Y. and Wu,Y.
TITLE USP47 stabilizes YBX1 to promote the progression of acute myeloid
leukemia
JOURNAL Oncogene 43 (7), 539-542 (2024)
PUBMED 38104157
REMARK GeneRIF: USP47 stabilizes YBX1 to promote the progression of acute
myeloid leukemia.
REFERENCE 2 (bases 1 to 10275)
AUTHORS Wang,A., Huang,H., Shi,J.H., Yu,X., Ding,R., Zhang,Y., Han,Q.,
Ni,Z.Y., Li,X., Zhao,R. and Zou,Q.
TITLE USP47 inhibits m6A-dependent c-Myc translation to maintain
regulatory T cell metabolic and functional homeostasis
JOURNAL J Clin Invest 133 (23), e169365 (2023)
PUBMED 37788092
REMARK GeneRIF: USP47 inhibits m6A-dependent c-Myc translation to maintain
regulatory T cell metabolic and functional homeostasis.
Publication Status: Online-Only
REFERENCE 3 (bases 1 to 10275)
AUTHORS Shin,S.C., Park,J., Kim,K.H., Yoon,J.M., Cho,J., Ha,B.H., Oh,Y.,
Choo,H., Song,E.J. and Kim,E.E.
TITLE Structural and functional characterization of USP47 reveals a hot
spot for inhibitor design
JOURNAL Commun Biol 6 (1), 970 (2023)
PUBMED 37740002
REMARK GeneRIF: Structural and functional characterization of USP47
reveals a hot spot for inhibitor design.
Publication Status: Online-Only
REFERENCE 4 (bases 1 to 10275)
AUTHORS Chen,H.Y., Tang,R.C., Liang,J.W., Zhao,W., Yu,S.S., Yao,R.R.,
Xu,R., Zhang,A., Geng,S., Sun,X.Y., Ge,Q. and Zhang,J.
TITLE Deubiquitinase USP47 attenuates virus-induced type I interferon
signaling
JOURNAL Int Immunopharmacol 118, 110040 (2023)
PUBMED 37001379
REMARK GeneRIF: Deubiquitinase USP47 attenuates virus-induced type I
interferon signaling.
REFERENCE 5 (bases 1 to 10275)
AUTHORS Kassel,S., Hanson,A.J., Benchabane,H., Saito-Diaz,K., Cabel,C.R.,
Goldsmith,L., Taha,M., Kanuganti,A., Ng,V.H., Xu,G., Ye,F.,
Picker,J., Port,F., Boutros,M., Weiss,V.L., Robbins,D.J.,
Thorne,C.A., Ahmed,Y. and Lee,E.
TITLE USP47 deubiquitylates Groucho/TLE to promote Wnt-beta-catenin
signaling
JOURNAL Sci Signal 16 (771), eabn8372 (2023)
PUBMED 36749823
REMARK GeneRIF: USP47 deubiquitylates Groucho/TLE to promote
Wnt-beta-catenin signaling.
REFERENCE 6 (bases 1 to 10275)
AUTHORS Yang,S.W., Oh,K.H., Park,E., Chang,H.M., Park,J.M., Seong,M.W.,
Ka,S.H., Song,W.K., Park,D.E., Baas,P.W., Jeon,Y.J. and Chung,C.H.
TITLE USP47 and C terminus of Hsp70-interacting protein (CHIP)
antagonistically regulate katanin-p60-mediated axonal growth
JOURNAL J Neurosci 33 (31), 12728-12738 (2013)
PUBMED 23904609
REMARK GeneRIF: The results of this study indicated that USP47 plays a
crucial role in the control of axonal growth during neuronal
development by antagonizing CHIP-mediated katanin-p60 degradation.
REFERENCE 7 (bases 1 to 10275)
AUTHORS Parsons,J.L., Dianova,I.I., Khoronenkova,S.V., Edelmann,M.J.,
Kessler,B.M. and Dianov,G.L.
TITLE USP47 is a deubiquitylating enzyme that regulates base excision
repair by controlling steady-state levels of DNA polymerase beta
JOURNAL Mol Cell 41 (5), 609-615 (2011)
PUBMED 21362556
REMARK GeneRIF: USP47 has a role in regulating DNA repair and maintaining
genome integrity
REFERENCE 8 (bases 1 to 10275)
AUTHORS Peschiaroli,A., Skaar,J.R., Pagano,M. and Melino,G.
TITLE The ubiquitin-specific protease USP47 is a novel beta-TRCP
interactor regulating cell survival
JOURNAL Oncogene 29 (9), 1384-1393 (2010)
PUBMED 19966869
REMARK GeneRIF: USP47, a novel beta-Trcp interactor, regulates cell growth
and survival, potentially providing a novel target for anticancer
therapies.
REFERENCE 9 (bases 1 to 10275)
AUTHORS Quesada,V., Diaz-Perales,A., Gutierrez-Fernandez,A., Garabaya,C.,
Cal,S. and Lopez-Otin,C.
TITLE Cloning and enzymatic analysis of 22 novel human ubiquitin-specific
proteases
JOURNAL Biochem Biophys Res Commun 314 (1), 54-62 (2004)
PUBMED 14715245
REFERENCE 10 (bases 1 to 10275)
AUTHORS Puente,X.S., Sanchez,L.M., Overall,C.M. and Lopez-Otin,C.
TITLE Human and mouse proteases: a comparative genomic approach
JOURNAL Nat Rev Genet 4 (7), 544-558 (2003)
PUBMED 12838346
REMARK Review article
COMMENT VALIDATED REFSEQ: This record has undergone validation or
preliminary review. The reference sequence was derived from
AC104383.4 and AC124276.5.
Sequence Note: The RefSeq transcript and protein were derived from
genomic sequence to make the sequence consistent with the reference
genome assembly. The genomic coordinates used for the transcript
record were based on alignments.
Publication Note: This RefSeq record includes a subset of the
publications that are available for this gene. Please see the Gene
record to access additional publications.
##Evidence-Data-START##
Transcript exon combination :: SRR1660803.150309.1 [ECO:0000332]
RNAseq introns :: mixed sample support SAMEA1965299,
SAMEA1966682 [ECO:0006172]
##Evidence-Data-END##
PRIMARY REFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP
1-253 AC104383.4 64816-65068
254-477 AC104383.4 93344-93567
478-537 AC104383.4 96644-96703
538-741 AC104383.4 103021-103224
742-855 AC104383.4 107311-107424
856-994 AC104383.4 114812-114950
995-1091 AC124276.5 194177-194273 c
1092-1237 AC124276.5 189010-189155 c
1238-1317 AC124276.5 188528-188607 c
1318-1467 AC124276.5 186322-186471 c
1468-1563 AC124276.5 171619-171714 c
1564-1716 AC124276.5 171376-171528 c
1717-1884 AC124276.5 168979-169146 c
1885-2016 AC124276.5 162305-162436 c
2017-2093 AC124276.5 161750-161826 c
2094-2149 AC124276.5 161119-161174 c
2150-2262 AC124276.5 158754-158866 c
2263-2367 AC124276.5 157935-158039 c
2368-2575 AC124276.5 155360-155567 c
2576-2688 AC124276.5 153498-153610 c
2689-2808 AC124276.5 151322-151441 c
2809-3586 AC124276.5 148758-149535 c
3587-3762 AC124276.5 143750-143925 c
3763-3843 AC124276.5 143312-143392 c
3844-3959 AC124276.5 141866-141981 c
3960-4078 AC124276.5 141388-141506 c
4079-4209 AC124276.5 138999-139129 c
4210-4257 AC124276.5 136926-136973 c
4258-4388 AC124276.5 136706-136836 c
4389-10275 AC124276.5 129983-135869 c
FEATURES Location/Qualifiers
source 1..10275
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/chromosome="11"
/map="11p15.3"
gene 1..10275
/gene="USP47"
/gene_synonym="TRFP"
/note="ubiquitin specific peptidase 47"
/db_xref="GeneID:55031"
/db_xref="HGNC:HGNC:20076"
/db_xref="MIM:614460"
exon 1..253
/gene="USP47"
/gene_synonym="TRFP"
/inference="alignment:Splign:2.1.0"
exon 254..477
/gene="USP47"
/gene_synonym="TRFP"
/inference="alignment:Splign:2.1.0"
misc_feature 427..429
/gene="USP47"
/gene_synonym="TRFP"
/note="upstream in-frame stop codon"
exon 478..537
/gene="USP47"
/gene_synonym="TRFP"
/inference="alignment:Splign:2.1.0"
CDS 517..4563
/gene="USP47"
/gene_synonym="TRFP"
/EC_number="3.4.19.12"
/note="isoform g is encoded by transcript variant 8;
ubiquitin carboxyl-terminal hydrolase 47; ubiquitin
thioesterase 47; deubiquitinating enzyme 47;
ubiquitin-specific-processing protease 47; ubiquitin
thiolesterase 47; Trf (TATA binding protein-related
factor)-proximal homolog"
/codon_start=1
/product="ubiquitin carboxyl-terminal hydrolase 47 isoform
g"
/protein_id="NP_001359024.1"
/db_xref="GeneID:55031"
/db_xref="HGNC:HGNC:20076"
/db_xref="MIM:614460"
/translation="MKKKFLQIENAAEEPRVLCIIQDTTNSKTVNERITLNLPASTPV
RKLFEDVANKVGYINGTFDLVWGNGINTADMAPLDHTSDKSLLDANFEPGKKNFLHLT
DKDGEQPQILLEDSSAGEDSVHDRFIGPLPREGSGGSTSDYVSQSYSYSSILNKSETG
YVGLVNQAMTCYLNSLLQTLFMTPEFRNALYKWEFEESEEDPVTSIPYQLQRLFVLLQ
TSKKRAIETTDVTRSFGWDSSEAWQQHDVQELCRVMFDALEQKWKQTEQADLINELYQ
GKLKDYVRCLECGYEGWRIDTYLDIPLVIRPYGSSQAFASVEEALHAFIQPEILDGPN
QYFCERCKKKCDARKGLRFLHFPYLLTLQLKRFDFDYTTMHRIKLNDRMTFPEELDMS
TFIDVEDEKSPQTESCTDSGAENEGSCHSDQMSNDFSNDDGVDEGICLETNSGTEKIS
KSGLEKNSLIYELFSVMVHSGSAAGGHYYACIKSFSDEQWYSFNDQHVSRITQEDIKK
THGGSSGSRGYYSSAFASSTNAYMLIYRLKDPARNAKFLEVDEYPEHIKNLVQKEREL
EEQEKRQREIERNTCKIKLFCLHPTKQVMMENKLEVHKDKTLKEAVEMAYKMMDLEEV
IPLDCCRLVKYDEFHDYLERSYEGEEDTPMGLLLGGVKSTYMFDLLLETRKPDQVFQS
YKPGVMVKVHVVDLKAESVAAPITVRAYLNQTVTEFKQLISKAIHLPAETMRIVLERC
YNDLRLLSVSSKTLKAEGFFRSNKVFVESSETLDYQMAFADSHLWKLLDRHANTIRLF
VLLPEQSPVSYSKRTAYQKAGGDSGNVDDDCERVKGPVGSLKSVEAILEESTEKLKSL
SLQQQQDGDNGDSSKSTETSDFENIESPLNERDSSASVDNRELEQHIQTSDPENFQSE
ERSDSDVNNDRSTSSVDSDILSSSHSSDTLCNADNAQIPLANGLDSHSITSSRRTKAN
EGKKETWDTAEEDSGTDSEYDESGKSRGEMQYMYFKAEPYAADEGSGEGHKWLMVHVD
KRITLAAFKQHLEPFVGVLSSHFKVFRVYASNQEFESVRLNETLSSFSDDNKITIRLG
RALKKGEYRVKVYQLLVNEQEPCKFLLDAVFAKGMTVRQSKEELIPQLREQCGLELSI
DRFRLRKKTWKNPGTVFLDYHIYEEDINISSNWEVFLEVLDGVEKMKSMSQLAVLSRR
WKPSEMKLDPFQEVVLESSSVDELREKLSEISGIPLDDIEFAKGRGTFPCDISVLDIH
QDLDWNPKVSTLNVWPLYICDDGAVIFYRDKTEELMELTDEQRNELMKKESSRLQKTG
HRVTYSPRKEKALKIYLDGAPNKDLTQD"
exon 538..741
/gene="USP47"
/gene_synonym="TRFP"
/inference="alignment:Splign:2.1.0"
exon 742..855
/gene="USP47"
/gene_synonym="TRFP"
/inference="alignment:Splign:2.1.0"
exon 856..994
/gene="USP47"
/gene_synonym="TRFP"
/inference="alignment:Splign:2.1.0"
exon 995..1091
/gene="USP47"
/gene_synonym="TRFP"
/inference="alignment:Splign:2.1.0"
exon 1092..1237
/gene="USP47"
/gene_synonym="TRFP"
/inference="alignment:Splign:2.1.0"
exon 1238..1317
/gene="USP47"
/gene_synonym="TRFP"
/inference="alignment:Splign:2.1.0"
exon 1318..1467
/gene="USP47"
/gene_synonym="TRFP"
/inference="alignment:Splign:2.1.0"
exon 1468..1563
/gene="USP47"
/gene_synonym="TRFP"
/inference="alignment:Splign:2.1.0"
exon 1564..1716
/gene="USP47"
/gene_synonym="TRFP"
/inference="alignment:Splign:2.1.0"
exon 1717..1884
/gene="USP47"
/gene_synonym="TRFP"
/inference="alignment:Splign:2.1.0"
exon 1885..2016
/gene="USP47"
/gene_synonym="TRFP"
/inference="alignment:Splign:2.1.0"
exon 2017..2093
/gene="USP47"
/gene_synonym="TRFP"
/inference="alignment:Splign:2.1.0"
exon 2094..2149
/gene="USP47"
/gene_synonym="TRFP"
/inference="alignment:Splign:2.1.0"
exon 2150..2262
/gene="USP47"
/gene_synonym="TRFP"
/inference="alignment:Splign:2.1.0"
exon 2263..2367
/gene="USP47"
/gene_synonym="TRFP"
/inference="alignment:Splign:2.1.0"
exon 2368..2575
/gene="USP47"
/gene_synonym="TRFP"
/inference="alignment:Splign:2.1.0"
exon 2576..2688
/gene="USP47"
/gene_synonym="TRFP"
/inference="alignment:Splign:2.1.0"
exon 2689..2808
/gene="USP47"
/gene_synonym="TRFP"
/inference="alignment:Splign:2.1.0"
exon 2809..3586
/gene="USP47"
/gene_synonym="TRFP"
/inference="alignment:Splign:2.1.0"
exon 3587..3762
/gene="USP47"
/gene_synonym="TRFP"
/inference="alignment:Splign:2.1.0"
exon 3763..3843
/gene="USP47"
/gene_synonym="TRFP"
/inference="alignment:Splign:2.1.0"
exon 3844..3959
/gene="USP47"
/gene_synonym="TRFP"
/inference="alignment:Splign:2.1.0"
exon 3960..4078
/gene="USP47"
/gene_synonym="TRFP"
/inference="alignment:Splign:2.1.0"
exon 4079..4209
/gene="USP47"
/gene_synonym="TRFP"
/inference="alignment:Splign:2.1.0"
exon 4210..4257
/gene="USP47"
/gene_synonym="TRFP"
/inference="alignment:Splign:2.1.0"
exon 4258..4388
/gene="USP47"
/gene_synonym="TRFP"
/inference="alignment:Splign:2.1.0"
exon 4389..10275
/gene="USP47"
/gene_synonym="TRFP"
/inference="alignment:Splign:2.1.0"
regulatory 4937..4942
/regulatory_class="polyA_signal_sequence"
/gene="USP47"
/gene_synonym="TRFP"
/note="hexamer: AATAAA"
polyA_site 4958
/gene="USP47"
/gene_synonym="TRFP"
/note="major polyA site"
regulatory 5304..5309
/regulatory_class="polyA_signal_sequence"
/gene="USP47"
/gene_synonym="TRFP"
/note="hexamer: AATAAA"
polyA_site 5333
/gene="USP47"
/gene_synonym="TRFP"
regulatory 5884..5889
/regulatory_class="polyA_signal_sequence"
/gene="USP47"
/gene_synonym="TRFP"
/note="hexamer: AATAAA"
polyA_site 5903
/gene="USP47"
/gene_synonym="TRFP"
regulatory 7519..7524
/regulatory_class="polyA_signal_sequence"
/gene="USP47"
/gene_synonym="TRFP"
/note="hexamer: AATAAA"
polyA_site 7542
/gene="USP47"
/gene_synonym="TRFP"
regulatory 7693..7698
/regulatory_class="polyA_signal_sequence"
/gene="USP47"
/gene_synonym="TRFP"
/note="hexamer: AATAAA"
polyA_site 7713
/gene="USP47"
/gene_synonym="TRFP"
ORIGIN
1 gacgaaggcg gctgtggtag cggcggcggc ggcggcggag ccctgggtcg gtgtctgcgc
61 gctggtgtct gaggcccagg ctgaggcctc cgctattgct ggagcgcagg cggcggagag
121 gatgactgcc gctgccattc tctcttgagc tagcgagccg ccgccaccct ccaccctccc
181 ccggcagggc ggagaggagc ggccggagtc agcgatggtg cccggcgagg agaaccaact
241 ggtcccgaaa gagaactgtc tttagctatt ccttcctgta gggtagttcc actggatgca
301 aattctttga gttttccttc atctgagaat gtcttgaatt ccacttgatt attaaagcaa
361 ttcctgggtc gttcttgaag ggcatttctg ctggttattg gattctggtt tgacggttat
421 tttttgtaag cacctgaaaa atactgtgcc acttccttct ggcctccgtg gtttctggat
481 gtgttttgga gatgcagaca aaatatcttt gatgaaatga agaagaaatt tttacagata
541 gaaaatgctg ctgaagaacc tagagtctta tgtattatac aagatactac taattcaaag
601 acagtgaatg aacggatcac tttaaattta ccagcatcta ctccagtcag aaagctcttt
661 gaagatgtgg ccaacaaagt aggctacata aatggaacct ttgacttggt gtggggaaat
721 ggaatcaata ctgctgatat ggcaccactg gatcatacca gtgacaagtc acttctcgac
781 gctaattttg agccaggaaa gaagaacttt ctgcatttga cagataaaga tggtgaacaa
841 cctcaaatac tgctggagga ttccagtgct ggggaagaca gtgttcatga caggtttata
901 ggtccgcttc caagagaagg ttctgggggt tctaccagtg attatgtcag ccaaagctac
961 tcctactcat ctattttgaa taaatcagaa actggatatg tgggactagt aaaccaagca
1021 atgacttgct atttgaatag ccttttgcaa acacttttta tgactcctga atttaggaat
1081 gcattatata agtgggaatt tgaagaatct gaagaagatc cagtgacaag tattccatac
1141 caacttcaaa ggctttttgt tttgttacaa accagcaaaa agagagcaat tgaaaccaca
1201 gatgttacaa ggagctttgg atgggatagt agtgaggctt ggcagcagca tgatgtacaa
1261 gaactatgca gagtcatgtt tgatgctttg gaacagaaat ggaagcaaac agaacaggct
1321 gatcttataa atgagctata tcaaggcaag ctgaaggact acgtgagatg tctggaatgt
1381 ggttatgagg gctggcgaat cgacacatat cttgatattc cattggtcat ccgaccttat
1441 gggtccagcc aagcatttgc tagtgtggaa gaagcattgc atgcatttat tcagccagag
1501 attctggatg gcccaaatca gtatttttgt gaacgttgta agaagaagtg tgatgcacgg
1561 aagggccttc ggtttttgca ttttccttat ctgctgacct tacagctgaa aagattcgat
1621 tttgattata caaccatgca taggattaaa ctgaatgatc gaatgacatt tcccgaggaa
1681 ctagatatga gtacttttat tgatgttgaa gatgagaaat ctcctcagac tgaaagttgc
1741 actgacagtg gagcagaaaa tgaaggtagt tgtcacagtg atcagatgag caacgatttc
1801 tccaatgatg atggtgttga tgaaggaatc tgtcttgaaa ccaatagtgg aactgaaaag
1861 atctcaaaat ctggacttga aaagaattcc ttgatctatg aacttttctc tgttatggtt
1921 cattctggga gcgctgctgg tggtcattat tatgcatgta taaagtcatt cagtgatgag
1981 cagtggtaca gcttcaatga tcaacatgtc agcaggataa cacaagagga cattaagaaa
2041 acacatggtg gatcttcagg aagcagagga tattattcta gtgctttcgc aagttccaca
2101 aatgcatata tgctgatcta tagactgaag gatccagcca gaaatgcaaa atttctagaa
2161 gtggatgaat acccagaaca tattaaaaac ttggtgcaga aagagagaga gttggaagaa
2221 caagaaaaga gacaacgaga aattgagcgc aatacatgca agataaaatt attctgtttg
2281 catcctacaa aacaagtaat gatggaaaat aaattggagg ttcataagga taagacatta
2341 aaggaagcag tagaaatggc ttataagatg atggatttag aagaggtaat acccctggat
2401 tgctgtcgcc ttgttaaata tgatgagttt catgattatc tagaacggtc atatgaagga
2461 gaagaagata caccaatggg gcttctacta ggtggcgtca agtcaacata tatgtttgat
2521 ctgctgttgg agacgagaaa gcctgatcag gttttccaat cttataaacc tggagtgatg
2581 gtgaaagttc atgttgttga tctaaaggca gaatctgtag ctgctcctat aactgttcgt
2641 gcttacttaa atcagacagt tacagaattc aaacaactga tttcaaaggc catccattta
2701 cctgctgaaa caatgagaat agtgctggaa cgctgctaca atgatttgcg tcttctcagt
2761 gtctccagta aaaccctgaa agctgaagga ttttttagaa gtaacaaggt gtttgttgaa
2821 agctccgaga ctttggatta ccagatggcc tttgcagact ctcatttatg gaaactcctg
2881 gatcggcatg caaatacaat cagattattt gttttgctac ctgaacaatc cccagtatct
2941 tattccaaaa ggacagcata ccagaaagct ggaggcgatt ctggtaatgt ggatgatgac
3001 tgtgaaagag tcaaaggacc tgtaggaagc ctaaagtctg tggaagctat tctagaagaa
3061 agcactgaaa aactcaaaag cttgtcactg cagcaacagc aggatggaga taatggggac
3121 agcagcaaaa gtactgagac aagtgacttt gaaaacatcg aatcacctct caatgagagg
3181 gactcttcag catcagtgga taatagagaa cttgaacagc atattcagac ttctgatcca
3241 gaaaattttc agtctgaaga acgatcagac tcagatgtga ataatgacag gagtacaagt
3301 tcagtggaca gtgatattct tagctccagt catagcagtg atactttgtg caatgcagac
3361 aatgctcaga tccctttggc taatggactt gactctcaca gtatcacaag tagtagaaga
3421 acgaaagcaa atgaagggaa aaaagaaaca tgggatacag cagaagaaga ctctggaact
3481 gatagtgaat atgatgagag tggcaagagt aggggagaaa tgcagtacat gtatttcaaa
3541 gctgaacctt atgctgcaga tgaaggttct ggggaaggac ataaatggtt gatggtgcat
3601 gttgataaaa gaattactct ggcagctttc aaacaacatt tagagccctt tgttggagtt
3661 ttgtcctctc acttcaaggt ctttcgagtg tatgccagca atcaagagtt tgagagcgtc
3721 cggctgaatg agacactttc atcattttct gatgacaata agattacaat tagactgggg
3781 agagcactta aaaaaggaga atacagagtt aaagtatacc agcttttggt caatgaacaa
3841 gagccatgca agtttctgct agatgctgtg tttgctaaag gaatgactgt acggcaatca
3901 aaagaggaat taattcctca gctcagggag caatgtggtt tagagctcag tattgacagg
3961 tttcgtctaa ggaaaaaaac atggaagaat cctggcactg tctttttgga ttatcatatt
4021 tatgaagaag atattaatat ttccagcaac tgggaggttt tccttgaagt tcttgatggg
4081 gtagagaaga tgaagtccat gtcacagctt gcagttttgt caagacggtg gaagccttca
4141 gagatgaagt tggatccctt ccaggaggtt gtattggaaa gcagtagtgt ggacgaattg
4201 cgagagaagc ttagtgaaat cagtgggatt cctttggatg atattgaatt tgctaagggt
4261 agaggaacat ttccctgtga tatttctgtc cttgatattc atcaggattt agactggaat
4321 cctaaagttt ctaccctgaa tgtctggcct ctttatatct gtgatgatgg tgcggtcata
4381 ttttataggg ataaaacaga agaattaatg gaattgacag atgagcaaag aaatgaactg
4441 atgaaaaaag aaagcagtcg actccagaag actggacatc gtgtaacata ctcacctcgt
4501 aaagagaaag cactaaaaat atatctggat ggagcaccaa ataaagatct gactcaagac
4561 tgactctgat agtgtagcat tttccctggg ggagttttgg ttttaattag atggttcact
4621 accactgggt agtgccattt tggccggaca tggttggggt aacccagtga caccagcact
4681 gattggactg ccctacacca atcagaagct cagtgcccaa tgggccactg ttttgactcg
4741 gaatcatgtt gtgcactata gtcaaatgta ctgtaaagtg aaaagggatg tgcaaaaaaa
4801 taaaaaaaaa caacaaaaaa agctaacctt ctattagaaa aggggacagg ggaatgagta
4861 aacttctttt attgcggaca aatgtgcaca tagccgctag taaaactagc ctcaaacagg
4921 atgctcatag cttaataata aaagctgtgc aaaggccatg aatgaatgaa ttttctgttt
4981 atttcactga tgcacacatt acctcattga caattcagaa gtaaatccaa cgtgtgttga
5041 ctcttggaaa gcagcaaaaa caggagctga agaaaagaaa ttcttggaac cagccgtaac
5101 ccagtaagga attgtgaagt tgtgttttta ttttgtttca ttttttgcag agtattaaga
5161 acattattct ggaacatcag aacgtttccc ttagaccgat cccagcaggt ggcagctcag
5221 attgctgcag tgttgtaatt ataactgatt gtacttaagt tatggatgta gagaatatgt
5281 ttcattcatt tattcagcat gtaaataaaa ttgatcctgt tgagttatca taattgcagt
5341 tcaactatct gccatgatta ttcttttcac gtatcattca ttctgtacat ttgtgtacat
5401 tgagaagtat agcaatctat gtaaatgtaa tcctcagtga ggttcctcag tgctaggtcc
5461 cataggattg tcgttgccct tgttaatgag gtttctctgt tcagcggctt caattttttt
5521 ctctttgtac atctagtttt gaagatttac ttcaagtttg aatcttctag aatgcttgta
5581 agtccagttt taatttttag agtcaatttg tagttacatg tagtttaact tttgggaaac
5641 gtcttaacat tgttctgaat aaacttgcta atgaggtcag gtcatggtac agactgatgc
5701 agtcaacatg atttcattgc agagtttatt agtatcagca agtttttgct ttgctaaata
5761 aaagtactca atgaacacaa ttctacataa attttgacat accatctaat ttataaaaat
5821 caataaaaaa ggttttggta aaactttttc atgccagatg ctgtttacaa caatgaacat
5881 gccaataaaa catttgttca ttctgttgtg ttattttagt cattaaactt ctgtggatga
5941 agaatctggg ttaagaatag atttgtcatc tttaaatatg acattttgta atgtgtattg
6001 gatatctcat ttctatgata aaggtatatt tacagtaaag ttctcataag agaaatgaaa
6061 agctgtgtta atatctaact ttggggaacc ctgtcagtat ttcagatccg atttttaccc
6121 tttttttctt ataagaaaga taaaattaga aaatactgtt agcaaatgtg gctctgccat
6181 ttgaatataa tcaccgagaa ttccatgtct taaaagtctc ctggaatcca caatgaaaaa
6241 aaaaatcttt tctaaggtat ttttctggct aatttttatt tgaagaaagc tatagcattt
6301 agcgaaattt gactgaagta atgttctgag tttgcattag tgggattggt gatgttctca
6361 gaagaaaatt ggaaacactt gtgatgaatt gtctttcaga tcacttagat tttctgatgt
6421 aagaggacag ctgtttggtt ctgatacagg cctgcttact tgggatgtag ggttagtaaa
6481 tggggtttct gctttaaagg actgacttgc tatcacacaa aagaggcaga cttgtaaaca
6541 caatgggctt tggagtttgg tctgattggg tttggtttag tattcctatg agcgtaaatg
6601 gtaaaattct tctgataccc actctttaga ctgtgccttc tgctctgttc tttgttttat
6661 gtttaactgc tgtttctaat tgcaggtgta ttacagatac aaataagagt aaagaaaata
6721 tatttcatta tagaaaagaa aaaattaaaa gcttcttgct tttcagtgcc tgatagagtg
6781 aaaacacaaa gttgcacttt aataatttca ataaaagcta atctgtgtca gcctccctct
6841 gcttcagaga gtcaggtgag catccataac ctaacaggca gagccctagc gatgtggatc
6901 aagtttcctg agcccggggg cggtggagcc tcatgatctc ttatcttttg aggctgaggc
6961 aggtcacatg caacaaattg tgaccctgct ccccacaagt catgcaaagg ttttgaagag
7021 cttttaccgt ggggcagatg aacttgtgtc aaccatgcac accctgtgag aaccaagtac
7081 ctgtgtttct aaggcgggca ctcaaggtga ggggtgcatt ctggccaaag aaacaaaagc
7141 tgtggtttca ggaccatgcc gtgtgtagct gatctgtacg ggacgtgtat gtaaggaaga
7201 gcaatcatga tagataagaa cagtgtgtga agcagccttc acactagagt gtttggtcat
7261 ctcttataat gtaagggaag gtactttaaa attctgggaa gatgcgatga actcatgtcc
7321 cagtcagaaa ataatccaat gaaataagca ttggttgcca ggccacagtt aggaattgta
7381 ttgtgataca tctagaggcc aagagagcag gagagagcta ccaacttaca ctgtggttta
7441 agctaaatga ccgcacagca tcatagcatt gcagtgttgt tactaaatct ggaagtgacc
7501 tgtgaatgta tggaatacaa taaagtcttt tattctggtt catttgctag tacttccttt
7561 ttgattggat actgtagttc ttcctctgga ttttattttg ttcagcgtca aggccctaat
7621 tttgcaaatg tagtctaaac cacattacgt ggactagagg atactctgaa ttagcaagtt
7681 ttttgtttgc tgaataaaac tattccatct taacaattct gtgtaatttt tgaaaaacat
7741 caacattttg gtcactagta gtcaaaatct tataaggcca tatgtcctga aaggcaaaac
7801 tctgtgtatg tgcgatttac cagggagata gtaggacagt ttttatgagg gtttcaaagg
7861 agttcatggc ccaaatgaag cttgtggcca cctaggttta gtgtacatga aattggaaga
7921 tgcttcttga ttgtagttcc catggggaag agggaggctg tgatcatttg gttagataca
7981 gcattaattg tcacaacttg actttcagaa acaagaatac tgcacaaact caagcatgca
8041 ttggagcatg tgttactatt tagaaaagag tgcagccatc agtaggtcaa gctaggaggg
8101 caaaagagag ttgagaggaa tgctctgccc cttccccatc acagcactgc ccctttccac
8161 ctcctcacca gctcacctgt acaagagaat tgatctcaac tgagtgtcag ctcaccaggt
8221 cagaaattgt tattggaccc taaaaccttg cctcacaggt gggcaccaag ctccccttcc
8281 ctctgctttc catgtttccc acctgccctc tccccacctg ttttcagcct cttttataat
8341 gcttaagtaa cctgcttgaa gagtataatt tatggagata aaaatactca atgcttacat
8401 ttttttcata actgtgccaa ttgttttgtc aaatgcagtt acccactggg cagtgggctt
8461 atcaagtgct cagtgcaatc ctgtgacttt taagtcattt tgcattatta agacaatctt
8521 atcttaaagt tcagtggttt cattcaaagc tcccctgaac ctccatagtg cctccaagat
8581 gtgctgtccc tgccaactcc tgcaccagct tgactggtgg tggaagaggc caggaagagc
8641 ggggggaaga catgtgctaa ccacttctta gcaatgaggc tggtaaggtt actgtcccct
8701 tgtgcctggt cactagtaac ttttttgtag acatgaaggt agatgccctg caggtcctat
8761 cagcaaagga cccaactcct aacagcattg gtgctccctc atgaaatttg acagtgtctt
8821 gccagcctcc tgctggcaga cacttctctg cagtgaatac cactatccca tgctgcacta
8881 gtccctacca agtcttcaac cctcaatttg atcttccatg agtgtctcaa aaagttcccc
8941 tcagaagttt cagccaaggg aataaaatct aacctctcct ccctcaacgg agtgtcaggg
9001 aggggaagaa tctgttttcc aaacaccaca atctcccaaa aggggatttc accctccctt
9061 ggtcatcttt atacagggca attgtgctgc tgctgctgag gtggccactt tcagcaagcc
9121 ctgtgtgcag gggcctagct gcatttagaa accaagcata gctatcaaaa agtaatctcc
9181 aaatagttga attgcatttc ttgagtatct ggagaggatg tgcagaccac aggaatacct
9241 aatgcctttt ttctcttcct gtctttgtcc ctcacactac agcaggcccc tcccttccct
9301 cttcaacctc atcctccctc cccacaggcc cagagaacca gttgggcttt gttctcctgc
9361 aggctatggt tcatcatgca aatagctcct gtgtcagaaa tgctttttgg cttcaaataa
9421 cagaaaagct aacaccagct ttatcaataa taatatcggt ggtttactta aggtgtccag
9481 agatggtgga gaacaggatt ggtttcctcc tcaatgtcaa ggactcaaag actctttctg
9541 tggtagggcc acatcctaaa ccctgtatcc tgtgattatt tacctgacag ggcaaaagag
9601 attttgcaga tgcaattaag gttaaggacc ttgacgtggg aagattgtga ttatttacct
9661 gacagggcaa aagagatttt gcagatgcaa ttaaggttaa ggaccttgac gtgggaagat
9721 tattctggat tatctaggtg ggcgcaattt gatcacatgg gtccccagaa gtggagaacc
9781 tttcccacct gtagaaagcc agagagctgg cacctgagaa ggacagaact gtcattgcag
9841 gatttgaaga tgaaggggcc catgagccaa ggaatgccag tgacctatag aggctaaaaa
9901 acagcaagga aatggactct ccccagagcc tccagaggaa tgcagccctg ttgatcacat
9961 gatcaccaga tggctgcccc agagccaaat gtcgcttcct gagcaccata ctcaaaggca
10021 ggggaagtgg atggagggca ggagctccat tcttgtttgc cactctcctt ttgtcaattg
10081 ggaaaaaatt ccagaaactc tgggagccct ccccttacat ttcctgggtc atggggccag
10141 ccctagctgc tggagggact gagaacagct gttgagcagt ttacctgacg gcatctgcca
10201 tggcttggca ggaactctgg ctttgggaga gagcagcagc aaggtattca agcaccacct
10261 ccacccagcc cctcc
//