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MAG: Candidatus Collierbacteria bacterium GW2011_GWC2_44_13 UW29_C0014, whole genome shotgun sequence

GenBank: LCHT01000014.1

FASTA Graphics 

LOCUS       LCHT01000014           12723 bp    DNA     linear   ENV 07-MAY-2015
DEFINITION  MAG: Candidatus Collierbacteria bacterium GW2011_GWC2_44_13
            UW29_C0014, whole genome shotgun sequence.
ACCESSION   LCHT01000014 LCHT01000000
VERSION     LCHT01000014.1
DBLINK      BioProject: PRJNA273161
            BioSample: SAMN03319758
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG.
SOURCE      Candidatus Collierbacteria bacterium GW2011_GWC2_44_13 (groundwater
            metagenome)
  ORGANISM  Candidatus Collierbacteria bacterium GW2011_GWC2_44_13
            Bacteria; Candidatus Collierbacteria.
REFERENCE   1  (bases 1 to 12723)
  AUTHORS   Brown,C.T., Hug,L.A., Thomas,B.C., Sharon,I., Castelle,C.J.,
            Singh,A., Wilkins,M.J., Williams,K.H. and Banfield,J.F.
  TITLE     rRNA introns, odd ribosomes, and small enigmatic genomes across a
            large radiation of phyla
  JOURNAL   Nature (2015) In press
REFERENCE   2  (bases 1 to 12723)
  AUTHORS   Brown,C.T., Hug,L.A., Thomas,B.C., Sharon,I., Castelle,C.J.,
            Singh,A., Wilkins,M.J., Williams,K.H. and Banfield,J.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAY-2015) Earth and Planetary Science, University of
            California - Berkeley, 307 McCone Hall, Berkeley, CA 94720, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: idba_ud v. 1.1
            Genome Coverage       :: 13.49x
            Sequencing Technology :: Illumina HiSeq2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12723
                     /organism="Candidatus Collierbacteria bacterium
                     GW2011_GWC2_44_13"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UW29_C0014"
                     /isolation_source="aquifer sediment"
                     /db_xref="taxon:1618391"
                     /environmental_sample
                     /geo_loc_name="USA: Rifle, CO"
                     /lat_lon="39.5369 N 107.7828 W"
                     /collection_date="Jun-2011"
                     /metagenome_source="groundwater metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="UW29_C0014G0001"
     CDS             complement(<1..192)
                     /locus_tag="UW29_C0014G0001"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA repressor"
                     /protein_id="KKT39398.1"
                     /translation="MITKRQKQVLDFVESYSQKKGYAPSFEEIRKRLKLASVSTIHFH
                     ISKLKEGGYLGNIENKARTI"
     gene            296..583
                     /locus_tag="UW29_C0014G0002"
     CDS             296..583
                     /locus_tag="UW29_C0014G0002"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="KKT39399.1"
                     /translation="MTKFSHTQKFTKENIVKVPQDKAVIYKIKDGGGENLYTGIAGRG
                     RSQERLLEHKEIKKEKIPDGTRFQFAQVKNKERAHIIEKQIIKKEQPEFNE"
     gene            595..1605
                     /locus_tag="UW29_C0014G0003"
     CDS             595..1605
                     /locus_tag="UW29_C0014G0003"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="KKT39400.1"
                     /translation="MQIQPSLQQAMDALKAINFKDLERTEELGRAYSFKQAVDFIKNV
                     YDDLLQVIENAELLRISTNTEQQITNLANQVNENVKKIQGFVLSGNEANAPSQHKQIN
                     EVVASLYQKDLELLPPLIERVNILKLNPSEVENKVAQAFQSIKEIEKIKGDAQKTKEN
                     IDSAVKEVRNALGKEGALISATDFEEQAKEHKNLSKKWFWGSIGSIGATAILVVILFS
                     GWLPSLNLINAGNDYPRIVQITVFKLVLLSIAYLLVYQSLKNYKINQHLYVLNRHRQL
                     TLSVYPLMAKATSEQEQSNAIVTQAAKAIFEPGTTGYLDGDDNPNPVNLTEIINRVVD
                     RK"
     gene            2048..2755
                     /locus_tag="UW29_C0014G0004"
     CDS             2048..2755
                     /locus_tag="UW29_C0014G0004"
                     /codon_start=1
                     /transl_table=11
                     /product="Regulatory protein, MerR"
                     /protein_id="KKT39401.1"
                     /translation="MLKISEAAEILGVSMDTLRRWDENGKLTAIKKEGGTHRYYREKD
                     MEIFASDLFKFASDWISQGAEFPGTFYCPTSAVFQARLVKMEHILMERPGFEKLYSLI
                     VLVAGEIGDNSFAHNLGKWPDTPGIFFGYDVQKGIIVLADRGLGILETLRRVRPDLSA
                     YVKAVEVAFTEFISGRAPEKRGNGLKLVREVVLANPIDLFFISGDAEVRMQGSDKVFR
                     VTRGQHVVRGCLAKIEF"
     gene            2844..3134
                     /locus_tag="UW29_C0014G0005"
     CDS             2844..3134
                     /locus_tag="UW29_C0014G0005"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="KKT39402.1"
                     /translation="MKVKLEKFGTTLISRELGSEAFKAFQPALLELPPNEELEIDFSS
                     ILTLSSSWADEFLSPLLEKLGERFVLLPSDNLSVQATLKILQEANQRPFKFR"
     gene            3277..3492
                     /locus_tag="UW29_C0014G0006"
     CDS             3277..3492
                     /locus_tag="UW29_C0014G0006"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="KKT39403.1"
                     /translation="MKRFKIKLLRTADGLNLQKGIPRKKSIVEVLHWCYTKAEAQRIA
                     SVIAGALTRSTHLGNIEYVVLTVRRHR"
     gene            complement(3544..4368)
                     /locus_tag="UW29_C0014G0007"
     CDS             complement(3544..4368)
                     /locus_tag="UW29_C0014G0007"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="KKT39404.1"
                     /translation="MTIEMRTENKNALLIAGDEYVYTYQPRDRFLKEIYKFGNFLQRI
                     GIPPLRIFNGTANEDTNYGINIMSSLADLTSSKEITSSTPLLIAYYGHGGKDGWYINE
                     SAFIPYYFLTRVISQYNGPVLLINDCCYSWSIIEHFTKYGIAQERVGVTPAAHKGEQY
                     FGQVVSRIAEYWQNGLYFEQPAGTGTMELTTVNIFDLGLKRPRTIRYEYTCCRENPKQ
                     LHLFRKKNPWVRRVMDPTAGKINTSTSTIREEIDLPRRWGARLDDLFFIQKQMTFL"
     gene            complement(4501..4881)
                     /locus_tag="UW29_C0014G0008"
     CDS             complement(4501..4881)
                     /locus_tag="UW29_C0014G0008"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="KKT39405.1"
                     /translation="MNEQENNLVFYETLVTIFWLLVDFFYIWELKIPLLFVAIPTIIF
                     ISLVLYKYSENLPMFLANCVMLGWGLMSVFWALDDLWHVPGALTFARCFVSFSVIILV
                     LFLIMGDFRREAVKRLRRIRVLKN"
     gene            complement(5161..5730)
                     /locus_tag="UW29_C0014G0009"
     CDS             complement(5161..5730)
                     /locus_tag="UW29_C0014G0009"
                     /codon_start=1
                     /transl_table=11
                     /product="Elongation factor P"
                     /protein_id="KKT39406.1"
                     /translation="MLTYTDLKKGVLFVKDGEPCEVVESNFSRMQQRKAVVQAKIRSL
                     RTGKIVDMSFQPSNNFEEATIDKKEINFLYHHRGDFVFTDPKNPKDRFTLKEDVIGDN
                     QKWLKPNTLITALMFNGELLNLKLPIKMDLKVTESPPGVQGDRSSSGTKSATIETGAV
                     IQVPLFINEGDVIRINTESGQYVERVEKA"
     gene            5982..6665
                     /locus_tag="UW29_C0014G0010"
     CDS             5982..6665
                     /locus_tag="UW29_C0014G0010"
                     /codon_start=1
                     /transl_table=11
                     /product="Conserved TM helix repeat-containing protein"
                     /protein_id="KKT39407.1"
                     /translation="MLIEPANWGDVIVFSLQQTWTSVVNFVPLLLGALIVLILGWIIA
                     LALGQLVEQIVRALKVDHFLMKLEVEKVLERGGLKLNAGAFVGGLVKWFFIVVFLLAA
                     VNILGLSDVSKFLSEVLAYIPNVVVAAVILIIAALVAEATEKVVRGSIEAAGHRGALA
                     GVVARWSIWVFAVIAALLQLKIVPELIQAVVSALVYGVALMFGLAFGLGGKDAAAQII
                     EKVRGELRR"
     gene            7275..12269
                     /locus_tag="UW29_C0014G0011"
     rRNA            join(7275..7689,7906..8170,8341..9931,10617..11319,
                     11988..12269)
                     /locus_tag="UW29_C0014G0011"
                     /product="23S ribosomal RNA"
     gene            9957..10046
                     /locus_tag="UW29_C0014G0014"
     ncRNA           9957..10046
                     /ncRNA_class="autocatalytically_spliced_intron"
                     /locus_tag="UW29_C0014G0014"
                     /product="self-splicing intron"
     gene            10107..10598
                     /locus_tag="UW29_C0014G0012"
     CDS             10107..10598
                     /locus_tag="UW29_C0014G0012"
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG homing endonuclease"
                     /protein_id="KKT39408.1"
                     /translation="MRSIEDYITGYVDGEGCFTVTFNRRPKMLIKWELRPSFSVSQNE
                     DRRQVLDVIREYFGCGYIRRDYSDKTVKFEIRDHKELMTKVIPHFGKHPFISAKQKDF
                     ELFSKICEMVDSGKHLTKGGFIEIIELAYQMNGSGKRRRTKEEIIKDLRSHQDEDIVL
                     SLS"
     gene            10571..10603
                     /locus_tag="UW29_C0014G0015"
     ncRNA           10571..10603
                     /ncRNA_class="autocatalytically_spliced_intron"
                     /locus_tag="UW29_C0014G0015"
                     /product="self-splicing intron"
     gene            11466..11912
                     /locus_tag="UW29_C0014G0013"
     CDS             11466..11912
                     /locus_tag="UW29_C0014G0013"
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG homing endonuclease"
                     /protein_id="KKT39409.1"
                     /translation="MSKSISLTINAYIAGFLDGDGSIYVRAKPNPTYRYGFQIAPYVV
                     FFQSEKSQEQFAKICKMIGGGYLRVRNDGILEYIVNKIDAIKKLLIAVHPFIVLKRKQ
                     IELMLKIIEQKATVKNEKDFKLLLELVDSYRELNYSKKRKKRTLTL"
     gene            11777..11981
                     /locus_tag="UW29_C0014G0016"
     ncRNA           11777..11981
                     /ncRNA_class="autocatalytically_spliced_intron"
                     /locus_tag="UW29_C0014G0016"
                     /product="self-splicing intron"
     gene            complement(12632..>12723)
                     /locus_tag="UW29_C0014G0017"
     CDS             complement(12632..>12723)
                     /locus_tag="UW29_C0014G0017"
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="KKT39410.1"
                     /translation="PQNNEEIFKLKNFCSDIPAKPTFPNPDLD"
ORIGIN      
        1 aatagtacgg gctttatttt ctatattacc taaatagcca ccttccttga gttttgagat
       61 atgaaaatga atagttgaaa cagaggcgag tttaagtcgc tttctaatct cctcaaatga
      121 gggggcatat cctttctttt gcgagtagct ttctacgaag tcaaggactt gtttttgacg
      181 ctttgtaatc atatacttta gatacttgac ttgtttcctt tacatcgatt ataatagaac
      241 aaaagtagaa aatcaaaacc gagttatccc caactagcat ttaactaaga tatctatgac
      301 aaaatttagc catactcaaa agttcaccaa agagaatatc gtcaaagttc cccaggacaa
      361 ggcggttatt tacaagataa aggatggggg tggagaaaac ctgtataccg gaattgccgg
      421 ccgcggccgt tcacaggaac ggttattgga gcataaggaa ataaagaaag agaaaatccc
      481 tgatggtacg cgattccagt ttgcgcaggt aaaaaataag gaacgcgctc atattataga
      541 aaaacagatc atcaaaaagg aacaaccaga gtttaacgaa taacataaat agccatgcaa
      601 atacaaccat cacttcaaca agcaatggat gcgctcaagg cgattaactt caaagacctt
      661 gaacgaacgg aggagctggg gcgtgcttat agcttcaagc aggccgttga tttcataaaa
      721 aatgtttacg acgacttgct acaggttatc gaaaatgcag aattactacg gatatccaca
      781 aatacggaac agcaaataac taatctggca aatcaagtca atgagaatgt aaaaaaaatc
      841 caagggtttg ttctttcggg aaacgaggca aacgcaccaa gtcaacacaa acaaataaac
      901 gaagtggttg caagtctata tcaaaaagat ttggaattac ttcctccgct tattgagaga
      961 gtaaatattc tcaagctcaa tccgtcggaa gtagaaaata aggttgctca agcctttcag
     1021 tcaatcaagg aaatcgagaa gatcaagggt gatgctcaaa aaacaaagga aaatatcgat
     1081 tcggcggtta aagaggtgcg caatgcactt ggaaaagagg gtgcactaat ctccgccacc
     1141 gattttgagg agcaggcaaa agagcataaa aatttatcaa aaaagtggtt ttggggatca
     1201 atcggctcca ttggagcaac cgctatttta gtagtgatcc ttttcagcgg ctggttacca
     1261 tctctaaatt taattaatgc ggggaacgac tacccgagaa tagttcaaat tacggttttt
     1321 aagctcgttt tgctatccat tgcttattta ttggtttatc agtcgcttaa aaattataaa
     1381 atcaaccaac atctgtatgt tttaaatagg catcggcaac tgactttatc agtttatcct
     1441 ttgatggcca aagccacgag cgaacaggag caatccaacg cgattgttac gcaagccgca
     1501 aaggcaatat ttgaaccagg aactactggt tatcttgatg gcgatgacaa tccgaatccc
     1561 gtgaatctga ccgaaattat caatagagtt gttgatagaa agtagtcgcc gccaaaaaat
     1621 tctcccgtcc gagcgttccg ccgagctaag cgaggcggtt atgtcagatt tcgctttttg
     1681 gatttgtgta tcgggtttgg caaaatgatt tcaaaagcat gccccgtggg gtcgcggagc
     1741 gactccttcg ggggtttatt cgggagccgc aggcaatcct tatcaaagaa atggagaaaa
     1801 tcggtattct taaagaaatt actggtttct cccgcaatcg gattttcgtt ttgcacgaat
     1861 acctagattt gttcaagaga taaggaaatc accaaaccaa tacgccaccc gcgtggcgta
     1921 ttgagataat gtgggctaat ccttaatttt cgccacattt tgcatagttt attgactttg
     1981 tgcatagatt ttagtgccta ttgcatagat tttaagtata tgataggata taaaaatgga
     2041 agaaaaattg ctcaaaatta gcgaagccgc cgagatttta ggcgtttcta tggacacgct
     2101 tcgtcgttgg gatgaaaacg gaaagttaac tgcgatcaaa aaagaaggcg gtacgcatcg
     2161 ctattaccgg gaaaaagata tggagatttt tgcaagcgat ctttttaagt ttgcgtctga
     2221 ttggattagt cagggagccg aattcccggg tactttttac tgccctacca gtgctgtgtt
     2281 tcaggcaagg ctggtaaaaa tggaacacat acttatggaa agaccgggct ttgaaaaatt
     2341 atactctttg attgttctgg tggccggaga aatcggggat aattcattcg ctcataatct
     2401 ggggaaatgg ccggatactc ccgggatttt ttttggttat gatgtccaaa agggcattat
     2461 tgttcttgcc gaccggggtt tgggtatttt agaaactttg cgtcgcgttc gtccggattt
     2521 atctgcgtac gtgaaggcgg tggaagttgc atttaccgaa tttatttctg gtcgggcgcc
     2581 ggaaaaacgc ggcaatgggc tgaaattagt tagagaagtg gtgctggcca atccgataga
     2641 cctatttttt attagcggtg atgctgaagt gcgcatgcaa ggttcggata aagtatttcg
     2701 tgttacgcgt ggtcagcatg tcgtgcgagg gtgtttggca aaaatagagt tttaataaat
     2761 aaaaatatat aaatcccgtt agaagtctcg cttgcgacaa ggtcgcaagt ccgaaggacc
     2821 tcgacggggt tacttctaac gggatgaaag taaaattaga aaaattcggt accacgctca
     2881 tatcccgcga attaggcagc gaggcgttca aagcgtttca gcctgcgcta ttagaactgc
     2941 cgccaaacga agaattggaa attgattttt ctagcatatt aacattatca tcatcatggg
     3001 cggatgagtt tttgagtcct ctgcttgaaa aattgggtga acggtttgtg ttattgccct
     3061 ccgacaatct ctcggtacag gcaaccctaa aaattctgca agaagcgaat cagaggccat
     3121 ttaaatttag atgaaatatg aagatgaata ctcgcggtga gcatagtcga accataaact
     3181 ccgatctaac acctgtccca aacgaaaatt gcaatataaa tagccgtttg ttaagttgat
     3241 cttagttttt ttaatagcca aaagggagta ttgccgatga aacgctttaa aatcaagttg
     3301 ctgagaactg ccgatggcct taatcttcaa aaggggatac cgcggaagaa aagtatcgta
     3361 gaggttctgc attggtgcta taccaaggcg gaagctcaga gaattgcaag tgttattgcg
     3421 ggggccttaa cccgcagtac ccatctcggc aatattgagt atgtcgtgct cacggtgcgg
     3481 cggcatcggt gatctgcgaa ctcttcatac aattaaaata agaggcgcta ttgcctctta
     3541 ttgttataaa aaggtcatct gtttttgtat aaagaaaaga tcatctagtc gtgctcccca
     3601 tctccgcggt aaatcgattt cttcacgaat agtacttgtt gacgtgttaa tttttccggc
     3661 agttggatcc attacccgtc tcacccatgg atttttcttt ctgaaaagat gtagctgttt
     3721 aggattttca cggcagcacg tatattcgta tctaattgtg cgagggcgtt ttagccccaa
     3781 atcaaatata tttaccgttg ttaattccat agtgcctgtg cctgcgggtt gttcgaaata
     3841 taaaccattt tgccagtatt cagcaatacg cgaaactacc tggccaaaat attgttcgcc
     3901 tttgtgcgcc gctggagtca ctccgactcg ttcttgtgcg ataccgtatt tggtaaagtg
     3961 ttcaatgatg gaccacgagt agcaacagtc attgattagc aaaaccggac cgttatattg
     4021 tgagataacg cgtgtcaaaa aataataagg tataaatgct gactcgttga tataccaccc
     4081 gtcttttccc ccatgtccat aataggcgat aagtagcgga gtgctgctag tgatttcttt
     4141 tgaggatgta aggtcggcta gggagctcat tatgttgatt ccgtagtttg tgtcctcatt
     4201 tgccgttccg ttaaatatcc ggagtggcgg aattccgatt cgttgcagaa agttcccgaa
     4261 tttataaatt tcttttagga atctgtcgcg aggctggtat gtgtaaacgt attcatcgcc
     4321 ggcgatcagc aatgcgtttt tgttttctgt gcgcatttct atcgtcataa tccccatctt
     4381 atttttgtaa agtgcctact atatagtacc atgtactttt tattttgtcc atcatcgtta
     4441 aaaaaataag aggcgctatt gcctcttttt ttgatttggc gcgaatcaga aaatatgtga
     4501 ttagtttttt agtacgcgaa tgcgtcttaa gcgtttaaca gcttctcgcc tgaaatcgcc
     4561 cataataaga aaaaggacta aaataattac actaaaagaa acgaaacatc tcgcaaatgt
     4621 tagtgcacca ggcacatgcc acaaatcatc taaagcccaa aatacactca taagccccca
     4681 tcccagcatt acgcagttgg cgagaaacat cggtaaattt tctgaatatt tataaagaac
     4741 taatgatatg aaaattattg tcggaatcgc tacgaacaat aaaggtattt tcagttccca
     4801 tatataaaag aaatccacta gaagccagaa aatcgtcact agtgtttcgt agaataccaa
     4861 attattttct tgttcgttca cgaatacccc attttttcag tttcagagtt tttatagcgt
     4921 atagtgttta tgtttatttg tcaaataaaa aaagcgcgta gcgctacatt aagaaaacca
     4981 tttaagcgac taatgcgatc gagacatttt gtgtagagcg acaatgggag gcggaatttg
     5041 cttggttcgt ttcgccggaa gcgaaacgaa aattccgccg acccttagtg aggcctccgc
     5101 cggagaggcc gaatcgtgtc gcgatacaca aaatgttgag atatcgcatt accgagcgag
     5161 ttaagctttt tccacccgct ccacatactg tccgctctcc gtattaatcc gtatgacatc
     5221 cccttcattt ataaatagtg gaacctgaat aacggcgcct gtttcaatgg tcgctgattt
     5281 ggttccggag gatgagcggt ctccttgaac tccgggcggg gattcggtga ctttcaaatc
     5341 cattttaatg ggaagtttta gattaagaag ttctccgttg aacataaggg cagtaataag
     5401 ggtatttggt ttgagccatt tttggttatc tccaattacg tcttctttca gagtaaatct
     5461 gtcttttggg tttttagggt cggtaaatac aaaatcgccc cgatgatggt acaggaaatt
     5521 tatctctttt ttatctatag tcgcctcttc gaaattattg cttggctgga aactcatgtc
     5581 cacgattttg ccggtgcgca ggctgcgtat tttggcctgc accacggctt tgcgctgctg
     5641 catgcgggag aagttggatt caaccacctc acatggctcg ccgtctttta cgaaaaggac
     5701 gccttttttt agatcggtgt aggttaacat taaggataaa tttttatctt ttatctttta
     5761 acttgcttcg gtgtggataa cccttgttta tcctaatact ttaaaccagt ataatgaaag
     5821 aatcaaggtt tgccttataa aaaggcgcaa aaaaggtcgg atcccgctag aatagcggga
     5881 tatatagcat taaaggttat tgtgcacaat aactattagt cagtgatatt aaataataat
     5941 aaataattat tctctaactt aaaactacat tgaataactc tatgcttatt gaacctgcaa
     6001 attggggaga tgtaattgtc ttttctctcc agcagacctg gacctcggtg gtcaactttg
     6061 tccctctgct tttgggagcg cttatagtat taatcctggg atggattatt gctttagcgc
     6121 ttggccagct ggtggagcaa attgtgcgag cgttaaaagt agaccatttc ttgatgaagc
     6181 ttgaagtgga aaaagttctt gagcgcggcg gtcttaaatt aaatgccggg gcttttgtgg
     6241 gaggacttgt gaagtggttt ttcatcgtgg tctttctgct cgccgcggta aatattttag
     6301 gacttagtga tgtttctaaa ttcttgagtg aggtgctggc ttatattccg aatgtcgtgg
     6361 tcgcggccgt gattttgatt atcgcggcgc tggttgcaga agccacggag aaagtggtgc
     6421 gcggctccat tgaggccgcc gggcaccgtg gagctctagc cggagttgta gcgcgctggt
     6481 caatttgggt atttgccgtt attgcggcgc ttcttcaatt aaagatagtg ccggaattga
     6541 ttcaggcagt cgtgagcgcg ctggtttatg gcgtggcact catgttcggc ttggcttttg
     6601 gcctgggcgg taaagatgcc gcggcgcaga ttattgagaa ggtgcgagga gaactaagga
     6661 gataatatgc gaatacggaa cgaattatcc ggcgaatata aacgaataat tctaaaaccc
     6721 gccaattggc gggttttaga attgaaactt gcgtttttta tatctccgga gttcggaata
     6781 atctagccga gatacttcgc catgagttta cgttaatacc aaagccctga tttgttcggg
     6841 gtatttttta tccacagtca agcctcttta ttcttattga cactatttaa gggcgagagt
     6901 ataattgatg ttacacatga gtattaacct acaatttatt caaaaagcgg ctcacatacg
     6961 aacctgaaca gccagtgttt tggtctgtgt gaataagaaa agccgcttat ctaagcggcg
     7021 tttatgtttt atatcggtcg tccggtgttc ttaaatcaag gataacggac ggttgataac
     7081 ggaatgaaac tttacaattt aatatcaggg aaaaaatatc ttaccccgca gcatctatgt
     7141 ggagatgaag cggggttatg cctttcaagt tttcaatgta atcccattag aagtccgatt
     7201 cgataaaatc gaatcggcag acgccttcgg cgtcttactt ctaacgggac gagaatttgg
     7261 gagggtgaag gaaaattaag agcatatggt ggatgccttg ggtcccgaag gcgatgaagg
     7321 acgtggcgtg cctgcgataa gcgtcgggga ggtggcgagc aacctttgat ccggcgatgt
     7381 ccgaatgggg aaaccccgcc gtgcaaaaca cggtgagccc ccatctaact atactgatga
     7441 tatagtattt atatatacat aggtttgaga aatggaaaat gttctttctc attctggttg
     7501 gtcggtatag ttaggtgggg gtggcgatac ccgctgaatt gaaacatctt agtaagcgga
     7561 ggaaaagaaa acaattcgag tttgtagttt atagtcggta gtttgtagtt ttctactcac
     7621 tactaactat ttattaccaa ctcgtccatt cccttagtag cggcgagcga aaagggaaat
     7681 agcccaaacc caccgtgcat agcactgaaa tccgaagcac gaaatccgaa atccgagaca
     7741 atatcaaaat cctaaattct aaattcaaaa cacgtttaga atttggaaca ttcgaatttg
     7801 aagtttgttt cgaatttcga tattcgaatt tcgagttttc gcgctgtaca cggcgggggt
     7861 tgtaagataa taacgctcgg ttaaattcga gaggagcaaa aagttgcttt atttagaaga
     7921 atgtccctgg aaaggacagc caaagagggt gatagccccg tatttgaaaa atattgcatg
     7981 ctcccagtta ttattcttga gtactgcccc gtatggagac tgggcaggaa acaggcagaa
     8041 ctagtctgcc aaggctaaat actctcggga caccgatagt gaactagtac cgtgagggaa
     8101 aggtgaaaaa tagcccgata agggcggtga aatagatctg aaaccatatg cttacaagga
     8161 gacatagccc gtgcctcact aatgttcggc acggaatgtc taatttctaa ttcacctaat
     8221 ttctaataaa atgcttaatg ttaaaaacag atttttgttt cggcaatttt aaatcttgaa
     8281 ttttattaga aattggaaat tagggtttag aaattcccgt agcgagcgat agcgaggtac
     8341 gggtagtgtc gtgcctattg aagaatgagc caacgagttt ttggatgcta ctcgtctaaa
     8401 tggcgtaaag ccatgaaggc atagggaaac cgagtgttaa tagcgcgtta tagtttatgg
     8461 tttgtagatg gtagtttgta gttttttcta ctcactactg cctaccgact ataaactaaa
     8521 tgtagtattc aaaagacccg aaaccagatg agcttgccat gagcagggtg aacgacgggt
     8581 aacaccgtcg ggaggcccga acccgttggt cgtacaacgc cttgggatga cttgtggtaa
     8641 ggagtgaaaa gctaatcgaa tctggaaata gctggttctc ttcgaaatag tttttggact
     8701 agccctgaat cacaccatac gcgaggtaga gctactgaat ggtctgtaaa gggcgaaagc
     8761 tcgacagacc aaccaaactc cgaatgcgcg tattgttatt tcaggagtca gcaggtgggg
     8821 gctaagcttc atcgtgcaag agggaaacag ccctgaccgt caactaaggt ccctaaatca
     8881 acgctaagtg taaaaaggtg gtggagtttc ttatacaccg aggaggttgg cttagaagca
     8941 gccatccttt aaagagtgcg taacagctca ctcgtctaga gattctgcgc ctaagattct
     9001 cggggctaag cgttgtaccg aagttacgga tgtgcggaat tttcgaattc cgtacgtagt
     9061 agaagagcat tgttatttca gtgaagccgc atccgtgagg agcggtggag agataacaag
     9121 agagaatgtt ggcacaagta accacaatgc cggtgagata ccggcacgcc gaaaacccaa
     9181 ggtttcctcg gcaatgaaaa tcaaccgagg gttagtcggt cctaaggcga tagcgaaagc
     9241 tgaagtcgat ggacagcagg ttaatattcc tgcaccgttg tattttttcg atggggtgac
     9301 ggagagtagt atgtcgggcg cactattgga tttgacgttt atgacgtaag gaatgctcgc
     9361 aggcaaatcc gcgagcaggg gttatgcccg attccaagcg tcgtgaaaat ctttggcagc
     9421 aatgccgaag aaatccgata aagcactctt ccaagaaaat cctctagagt caaaaataca
     9481 atgaccgtac cgtaaaccga cacaggtggg ttggtggaga acaccaaggc gaatgggtga
     9541 gtcctggtta aggaactcgg caaaaaagca gccgtaagtt cgcaatatgg cttaccccca
     9601 tcacattttg gattaagaaa atacaccaca aaagttctat tatgacttgt atcataatgt
     9661 caagtcgtat ccaaaatgtg gtgggggtcg cagcgaaagt aaccccgggc gactgtttac
     9721 caaaaacaca ggtccctgct aactcgtaag aggatgtata ggggctgacg cctgaccaat
     9781 gcgagaaggt taagcattct tgtggagtga tagcaatatt gttctgctct gggtgccaag
     9841 ccctcgtcaa tgtctgcggt aactataacc gtattaaagt agcgcaattc cttttcgggt
     9901 aaatttcgga atttgcccac taaaggagta attctttagt aaacagccag cttataacgg
     9961 tgaactcctt atggtaaaat tgctatatgg acaataccgt gggaagtcat ctgttcaaat
    10021 tttagcagat tgaccccgta acgactgagg caaaagccga aataaattgt ttataacagt
    10081 ttattatacg ctggcctctt gatgagatga gaagtattga agattacata actggatacg
    10141 ttgatggtga aggttgcttt acagtaactt ttaaccgacg tccaaagatg ttgattaaat
    10201 gggaattacg acccagtttt tctgtaagtc aaaatgaaga tcgacggcag gtgttagatg
    10261 taatccgtga atatttcgga tgcggatata ttaggagaga ttattctgat aaaacggtga
    10321 agtttgaaat ccgcgatcac aaagagttga tgacaaaagt tattcctcat tttggaaaac
    10381 atccatttat ctctgcaaaa cagaaggact tcgaattatt tagcaagata tgcgagatgg
    10441 tagattcagg aaaacatcta accaaaggcg gattcataga aattattgaa cttgcatatc
    10501 agatgaatgg atcaggaaaa cgcagaagaa caaaagagga aattataaaa gatcttagat
    10561 ctcatcaaga tgaagatata gtcttatctt tatcgtaaga taaagcatta atatgaagtt
    10621 ccgaagcgca cgaaaggcgt aacgacttgg ggactgtctc aaccaggagc ccagtgaaaa
    10681 tacagtaccg gtgaagatgc cggttccctg tggttagacg aaaagacccc agaagcttta
    10741 ctgcagcttg atattgagtt taaagttttc ctgcgtagca tagatgggag gctttgaagt
    10801 cctaatttcg gttgggatgg agccgtcagt gaaataccat tcttggaaat tttaaattct
    10861 aaccgtcgcg ggacctaaaa aaggaaatcg cggcgagaca gtgtctggtg ggcagtttgt
    10921 gtggggcaca atcctcccaa aaagtaatgg aggagcctat taaggtcggc ttggagcgga
    10981 tggacatcgc tctggacgtg caaaggcata agccggctta actgcaaggt ggatataccg
    11041 cgcagttgcg aaagcagagc ttagtgaacc gatggcaatg gtatgtattc acgcttcggg
    11101 ttttcccgat gtgtgggtat ttacttctta ctagaataag gccaaagatt aacagataaa
    11161 agctactctg gggataacag gctagttccg cccgagagtc cttatcgacg gcggagttcg
    11221 gcacctcgat gtcggctcaa cttatcccgg ggctggaaaa ggtcccaagg gtttggctgt
    11281 tcgccaatta aaaagttacg tgagctgggt tcagaccgtc aaaatgaatc attaatatcg
    11341 agaatcacac ggcggctttc tcgcgtaagc gggatcgaat aaaccgactc atatcggtga
    11401 agtcctgata atatgttgca tgagcaacct gtcatatgtt aggataatac cgagggaagt
    11461 cgtgtatgtc aaaatcaatt tcattaacta taaacgcgta tatcgcagga tttcttgacg
    11521 gtgacggaag tatttatgtg cgtgcgaaac cgaaccctac ttatcggtat ggatttcaga
    11581 ttgcaccata tgttgtattc tttcagtcag aaaaaagtca agagcaattt gctaaaatat
    11641 gtaaaatgat cggcggtgga tatttacgag tacgaaatga tggaattctg gagtatatag
    11701 taaataaaat tgatgcgatt aagaaattgc ttatagcagt tcatccgttt atagtattga
    11761 agagaaaaca aattgaacta atgcttaaaa ttattgagca aaaggcaact gttaaaaatg
    11821 aaaaagattt taagctcttg cttgaactag ttgattcata tcgagaatta aactattcaa
    11881 aaaagaggaa aaagcgtaca ttgaccctgt agagactaca cgtcggtctc ccgatcaaaa
    11941 tcgggatggt ggtatagtcc gacaccttta gtaatagagg aaacagaaag cgtgagacag
    12001 gttggtctcc tatctaccac aggcgcaaga tacttgaggg gattcgtccc tagtacgaga
    12061 ggaccgggat gaacgaacct ctggcgtacc agctgttctg ccaagagcat cgctgggtac
    12121 ctatgttcgg aattgataag cgctgaaagc atctaagcgc gaaacatacc ccaagattag
    12181 gtatcataga tccgtagaag atgactacgt tgatagggtt taggtgtaag ccgagtaatc
    12241 ggttaagccg agaactacta atagatcata tttccttcac ccttccaagt tttcgttcgc
    12301 aatcgcactt tttgttttat ttgctcggta acacggtttt tcgacacgga cgattttgcc
    12361 cagcggcaaa atcgcctaag ggtcgagccg aatttcgtcc cttgatagac tcgggacgaa
    12421 ccatgcaatt cggctctccc attgtctcaa aaccgtatta cctcgctgta aaaaagtgcg
    12481 attgactcac tataagggtt ttcataatgt agccgctttt agcggcatgc tgataaaaat
    12541 ttctttagtg atttcccggt gatttagcct atcgtgtacc actccttccc attccgaaca
    12601 ggaccgtgaa agcgataggc actgatgata cttagtccaa atccggattt gggaaagtag
    12661 gttttgccgg gatatcactg cagaaatttt tcagtttaaa aatctcctcg ttattttgag
    12721 gag
//
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