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MAG: Candidatus Giovannonibacteria bacterium GW2011_GWA1_43_15 UV75_C0013, whole genome shotgun sequence

GenBank: LCFR01000013.1

FASTA Graphics 

LOCUS       LCFR01000013           10719 bp    DNA     linear   ENV 07-MAY-2015
DEFINITION  MAG: Candidatus Giovannonibacteria bacterium GW2011_GWA1_43_15
            UV75_C0013, whole genome shotgun sequence.
ACCESSION   LCFR01000013 LCFR01000000
VERSION     LCFR01000013.1
DBLINK      BioProject: PRJNA273161
            BioSample: SAMN03319704
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG.
SOURCE      Candidatus Giovannonibacteria bacterium GW2011_GWA1_43_15
            (groundwater metagenome)
  ORGANISM  Candidatus Giovannonibacteria bacterium GW2011_GWA1_43_15
            Bacteria; Candidatus Giovannonibacteria.
REFERENCE   1  (bases 1 to 10719)
  AUTHORS   Brown,C.T., Hug,L.A., Thomas,B.C., Sharon,I., Castelle,C.J.,
            Singh,A., Wilkins,M.J., Williams,K.H. and Banfield,J.F.
  TITLE     rRNA introns, odd ribosomes, and small enigmatic genomes across a
            large radiation of phyla
  JOURNAL   Nature (2015) In press
REFERENCE   2  (bases 1 to 10719)
  AUTHORS   Brown,C.T., Hug,L.A., Thomas,B.C., Sharon,I., Castelle,C.J.,
            Singh,A., Wilkins,M.J., Williams,K.H. and Banfield,J.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAY-2015) Earth and Planetary Science, University of
            California - Berkeley, 307 McCone Hall, Berkeley, CA 94720, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: idba_ud v. 1.1
            Genome Coverage       :: 15.78x
            Sequencing Technology :: Illumina HiSeq2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10719
                     /organism="Candidatus Giovannonibacteria bacterium
                     GW2011_GWA1_43_15"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UV75_C0013"
                     /isolation_source="aquifer sediment"
                     /db_xref="taxon:1618644"
                     /environmental_sample
                     /geo_loc_name="USA: Rifle, CO"
                     /lat_lon="39.5369 N 107.7828 W"
                     /collection_date="Jun-2011"
                     /metagenome_source="groundwater metagenome"
                     /note="metagenomic"
     gene            100..669
                     /locus_tag="UV75_C0013G0001"
     CDS             100..669
                     /locus_tag="UV75_C0013G0001"
                     /codon_start=1
                     /transl_table=11
                     /product="Peptidyl-prolyl cis-trans isomerase"
                     /protein_id="KKS99014.1"
                     /translation="MKQNYIIAIIVLLILGAGGYFVFIKNNMETPKIQKRTVTIETNM
                     GTIKFETYDADAPKTVQNFITLAQKGFYDNLIFHRVINGFMIQGGDPLGNGTGGPGYQ
                     FEDELNPATPSFKAGYKRGVVAMANAGPNTNGSQFFIMLADNPLPHNYTIFGKVISGQ
                     DVVDKIGQTPTGPQDRPIEPVIIKNVKVE"
     gene            737..1168
                     /locus_tag="UV75_C0013G0002"
     CDS             737..1168
                     /locus_tag="UV75_C0013G0002"
                     /codon_start=1
                     /transl_table=11
                     /product="Membrane protein-like protein"
                     /protein_id="KKS99015.1"
                     /translation="MSKPLRNINIESQEKLNKLDRLAFFITKKVGSPGFFLIIFLWTI
                     FWIGWNILAPDTLRFDPYPAFVLWLFISNMIQILLMPLIMVGQNLQGRHAEFRAEADF
                     EVNTKAELEIETILLHLERQNELILKILNHLENEKGSKNVQ"
     gene            1271..2416
                     /locus_tag="UV75_C0013G0003"
     CDS             1271..2416
                     /locus_tag="UV75_C0013G0003"
                     /codon_start=1
                     /transl_table=11
                     /product="Subtilisin"
                     /protein_id="KKS99016.1"
                     /translation="MSVFALVSAVSARVTVDKEDSHKRVIAHNEEEESKALSSGCKVV
                     REAKTLKALRCPQAVAASLSLAEDIKVFALDDEEVDILRSPRPSAASASANQQIGATI
                     VHNNGNTGNGRKVVVLDTGYNRLHPELSSSYLGGKDFVNNDNDPTDDNGHGSHVAGII
                     TADGIDPKAKGVAPDAGIIAGKVLDENGSGYFSDVVAAIYWAINGSDGIYGNGDDFGA
                     DAINLSLGTVRTYKGFCDSALPDLTNAISYAKNKGILVVVAAGNSGNAGVSIPGCISY
                     STTVGAVNSSDYVASFSGRGNALDITAPGVSIYSSVLLNVYATWSGTSMATPMISGVV
                     ALIKSAHPTYTQVQAENKLFTTAKDLGKFGKDKDYGWGRVRANLAVQ"
     gene            complement(2434..3678)
                     /locus_tag="UV75_C0013G0004"
     CDS             complement(2434..3678)
                     /locus_tag="UV75_C0013G0004"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="KKS99017.1"
                     /translation="MIKKRKIKKKIKSKKTVKKPPAKKTPAKKITKEGSRAKASLPSK
                     AELKKEFSEERVEILLKKGKERGFLTYPEILSAFPHVEYNVVFLEDLYGRLESAGVDV
                     LEGRELLELPQSVQAKEEKPAKKKAGRRALVLDQDITAADSVQMYLKEIGRIPLISAD
                     EEKELARRIEAGDEDAKNRLAQANLRLVVSIAKKYVGRTPHLTMLDLIQEGNLGLFRA
                     VEKFDWRKGYKFSTYATWWIRQAVTRAIADQGKTIRIPVHMVETISKYQQVRRRLTQD
                     LGRDPLAEELAAEMNIEVEKIHHIQKISQDTVSLEAPVGEDDEDSTLGDFIEDKQMLT
                     PAQEAARKLLKDHIKEILVDLMPREYKILRMRFGLDDGISHTLEEVGKEFGVTRERIR
                     QIEAKAIERIRQHHKVIKLHEY"
     gene            complement(3675..5372)
                     /locus_tag="UV75_C0013G0005"
     CDS             complement(3675..5372)
                     /locus_tag="UV75_C0013G0005"
                     /codon_start=1
                     /transl_table=11
                     /product="primase protein"
                     /protein_id="KKS99018.1"
                     /translation="MSSQIDEIKNRLDIAELVGSYIRLQKAGVNFKALCPFHNERTPS
                     FNVSPARQIWHCFGCGKGGDMFEFIQQIEGVEFGDALKTLADRAGVELKREDPRLKSE
                     RTRQFALLEEACKFFESNLAVKPPLGGLTALDYLKKRGLQDETIKTFRLGYASDEWRA
                     LGAHLKQKNFSEKDLENSGLVVKSGQGYYDRFRGRIMFPIFDYSGRVVAFGGRILEAP
                     TSQAVGATEAVAKYVNSPETSLYQKSKILYGLNFAKSEILRASECVLVEGYMDVIMSW
                     QGGVKNVVAASGTALTHDQLKTIRRLAEKLIVAFDMDSAGEGASKRGIDLALAGGFDV
                     RVAGALDAGLKDPADAVYKNPALWQKAVSASRHIAQFYIDSALKKHKPNSPEAKREFQ
                     RTVLPVISSLADLERAHWVREVSRILNIKEEAVWSASEKNKPAEGTENVFENSASKPK
                     TRKELLEEKVLGIVAEYPALVPKLDPALSALISSGSDGTVVAGGRTAIFAELFLSGII
                     DAEAELIKCQRELKKEYLKEKLAYLNAQISSAEKTGEGNVGDLLGEFQKISKELHTL"
     gene            complement(5402..6484)
                     /locus_tag="UV75_C0013G0006"
     CDS             complement(5402..6484)
                     /locus_tag="UV75_C0013G0006"
                     /codon_start=1
                     /transl_table=11
                     /product="Copper amine oxidase-like protein
                     domain-containing protein"
                     /protein_id="KKS99019.1"
                     /translation="MILKFALAVFLILAPSGAYAKTATPPLEISGWIPYWRTATGTAD
                     ALAHIDSFKEINPFGYTVKNEGTLFDAMKIDEEPWTTLIAEAKKRKIRIIPTVMWSNT
                     EAIHRILSNQKTRIALEDEIALLVKEKGFDGIDIDFEGKKAETKNYFSTFLKGLYQRM
                     GKKWVMCTIEARTPLDSRYDTIPKDIRYANDFVAINKYCDRVRLMTYDQGTIDLRLNK
                     SALGPYIPIADAKWVEKVVNLAAKNISKKKLIIGVATYGYEFDVKPLAEEGFRYNLIS
                     AFNPRYALDLAKDLGVFPQRNIAGELSFMYISTTTESSSLKLLWWSDAVAIKDKINLA
                     KKLGVRGIAIFKIDGGEDPAIWDILK"
     gene            complement(6481..6876)
                     /locus_tag="UV75_C0013G0007"
     CDS             complement(6481..6876)
                     /locus_tag="UV75_C0013G0007"
                     /codon_start=1
                     /transl_table=11
                     /product="putative disulfide formation protein"
                     /protein_id="KKS99020.1"
                     /translation="MLLAFLAATASLILSLFYSDYAGYEPCKLCWLQRIFIYPQVILL
                     GMALYRKDYKIIDYALALLFVGTVVSLYHNYLSFGGSPFFNCDALQLSVSCAKRYVFE
                     LGYITIPIMSLTSFLLMIFFLKIQKLYNK"
     gene            complement(7013..7423)
                     /locus_tag="UV75_C0013G0008"
     CDS             complement(7013..7423)
                     /locus_tag="UV75_C0013G0008"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="KKS99021.1"
                     /translation="MQIKNIAIWIAAGAAIIVGGAWFLMKADSSPSKYDDFTKCLKDK
                     GAIFYGAFWCPHCQNQKKMFGSSEKFLPYVECSTPDGRSQLPLCNDKKISGYPTWEFA
                     DGSRESGEVPLETLSKKTSCPLRQSESEVSPLPK"
     gene            complement(7445..8143)
                     /locus_tag="UV75_C0013G0009"
     CDS             complement(7445..8143)
                     /locus_tag="UV75_C0013G0009"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="KKS99022.1"
                     /translation="MKTKEGLWKLSPSGLYGYTECRSCFWADNHYKKAPMLPLLLNSA
                     MDSILKARYDKYRADGAFPPEAKELEKEGIKPFEDIDTLNEWRAKTGALKVVDEGAGY
                     ELTGKIDDVLVEADGRLIPADYKSSGNAPKEDKQKYYRDQLAAYGFMFKKHGRKVSDR
                     AYLLHYFVKDKSNPSIEVKFASHVDLVKIDLGAIEQKLADMAKLLNKSYPGHNEDCGK
                     CSYHNGRGGIFDIT"
     gene            complement(8170..>10719)
                     /locus_tag="UV75_C0013G0010"
     CDS             complement(8170..>10719)
                     /locus_tag="UV75_C0013G0010"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="KKS99023.1"
                     /translation="ISQPEPTPLVSEIPDNAFQINLAVKPPSEEIVGAKSDLDVGRLS
                     EISRSSTLGVSPGGVEEENHSNILENVGMSQVGGNTESGSLPPFLQLQAIGGGSAGFG
                     GGGSPTPSVQILTTSSEVQSLGGTETSEPPDTTPPDISLSVSECSSSLSSDGCLLASS
                     TVSLVWSSTANDLNHYIIECEKSSPPVGGCSGFNFASTTATSTIYSLPTDDSIYIFKA
                     KAVDGAGNESAQTTKTAELSLRPVVINEVAWAGTSAPFANDEWIELYNRSSQSVNLAN
                     WVLYASDGAPYINLLGAVSARGYYLIERTNDNTVSDVAADLIVPFSGLSSGSGLGNSG
                     EILILSKASTTIDQTALCPGRWCGGAAGGVYSTMERIDPDIAGTDTTNWSTSNSGASN
                     SFIKNGKDAVGANLTATPRARNSLHYLISQNSTLSADRVLKKSWGTYIIRPSENFTVP
                     AGKTLTIEPGVTVKMGDTADLIVNGTLKSDGTAENNILFTSLSSPPFSGSWRNVRITS
                     SSQNSSVSYTRFKYGGGFTSNIPLERRALFSVIDNSVTISNSIFESSFSVGMRLITSN
                     STVQNSIFSVGTTTTDNVGLYIIGGSPTVSGNTFLENYYGFKAEGGSASVSDNVFTNN
                     ISYAAYSINGASTFSGNSGAGNGKNGIALSGTISTASGTTTIYANAIPYLIGEYPYEN
                     PKVAAGSAAIIETGAVWKGLTPYSRFVVNGALKLEGANKDSIIFTSVADSAPAQWYGM
                     EVNAGGYLYGGGFTLRYGGRGDGGNPNAIAGIGVFGGSVNLSDGRIENNYLAGMRLYD
                     RATSTLTNFEFNNHTAPTGNSTAFISANSTIQATNVTFSGNTTDKSPANAF"
ORIGIN      
        1 tatatagtat attattgaag tattccccgg tagctcagcg gtagagcggc gccctgttaa
       61 ggcgacggtc gcaggttcga tccctgcccg gggagccaga tgaaacagaa ttatataata
      121 gcaattatag ttttattgat tctgggagcg gggggatact tcgtttttat taaaaataat
      181 atggaaacgc ctaaaatcca gaagcgcaca gtcacgattg aaacaaatat gggaacgata
      241 aaatttgaaa catacgacgc cgacgcgccg aaaacggttc agaattttat cacgctagcc
      301 caaaaaggtt tttatgataa tcttattttt caccgagtta tcaatggctt tatgatccaa
      361 ggaggagatc cgttgggcaa tggcacggga ggcccgggat atcaatttga agatgaatta
      421 aaccccgcga ctccgtcttt taaagctggg tataaaaggg gcgttgtggc tatggccaac
      481 gccggtccaa ataccaacgg cagccagttt tttatcatgc ttgcggataa tcccctgcct
      541 cacaattaca caatttttgg aaaagtaatt tccggccagg acgttgtgga taaaatcggc
      601 caaacgccca ccggccccca agaccggccc atagaaccag tcatcataaa aaacgtgaaa
      661 gttgagtaaa aattcaacgc tatgttaaaa tagtcatatg gcgcaaaagc cgaaaacttt
      721 gcaggagctt agggctttga gcaagcccct gcggaatatt aatatagaga gccaagaaaa
      781 gctgaacaag ctggacaggc tcgcgttttt tatcaccaaa aaggtcggct cgcctggctt
      841 ttttttgata atatttcttt ggacaatttt ttggatcggc tggaatattt tagctccgga
      901 tactttgcgc tttgaccctt atccggcttt cgtcctttgg ctttttattt ccaatatgat
      961 tcagatactt ttaatgccct tgattatggt ggggcaaaat ttgcaggggc ggcatgcgga
     1021 attccgcgcg gaagctgatt ttgaagtaaa cactaaagcc gaactggaaa ttgaaaccat
     1081 ccttctccat ttggaacggc aaaacgaact gattttgaaa atcctcaatc atctagaaaa
     1141 cgagaaaggg agtaaaaacg ttcagtaaag ttatcaacaa atggtattga cagaagataa
     1201 tcgggtttga tacacttaga ttatgactaa attttcaagt tttaataagt ttgtagcggc
     1261 ggttgcctta ttgtccgttt tcgctcttgt gagcgcggtg agcgcccggg taaccgttga
     1321 taaagaagat tcgcataaaa gagtaattgc tcataacgaa gaagaggaga gcaaagcgct
     1381 ttcttctggt tgcaaagttg taagagaagc aaagacatta aaagctcttc gttgtccgca
     1441 ggcagtagca gcatctctat ctctcgccga ggacataaaa gtttttgctt tagacgatga
     1501 ggaagtagac attttgagaa gtccccggcc cagcgcggcg agcgccagcg ctaatcagca
     1561 aatcggcgct actattgttc acaacaatgg gaacactgga aatggcagaa aggtggtggt
     1621 tttagacact ggttacaatc gtcttcaccc agaactaagc tctagttatt taggaggaaa
     1681 agattttgtt aataatgata atgacccgac ggacgataat ggccatggca gtcatgtggc
     1741 gggaattatt accgccgacg gaattgatcc taaagcaaaa ggcgtggctc ctgacgcggg
     1801 aattatcgcc ggtaaggtgc ttgatgaaaa cggctccggc tattttagcg atgttgtggc
     1861 cgcgatttat tgggcgataa acggttcgga cggtatttac ggaaatggcg atgatttcgg
     1921 cgctgacgca attaacttga gtttaggtac cgttaggact tacaaaggtt tctgtgacag
     1981 cgccttgcct gatttaacaa acgccattag ctacgctaag aataagggga ttctggttgt
     2041 ggtagctgcc ggtaatagcg gaaacgcagg cgtttctatt cccggttgca tcagttattc
     2101 aaccaccgtt ggcgccgtta atagcagtga ttatgtcgcc agcttttccg gcagaggcaa
     2161 tgctttggat attacagctc cgggagtaag tatttattcc agcgttttgt tgaatgtcta
     2221 tgccacttgg agcggaacaa gtatggcaac tccgatgata agcggcgtgg tagcgcttat
     2281 taaaagcgct catccgactt acactcaagt ccaagcagag aataagttat ttactactgc
     2341 caaagattta ggtaagtttg gcaaagataa agattacggt tggggcagag tgagagcaaa
     2401 tctggctgtt caataaattc aagcgactaa aaatcaatac tcgtgcaatt ttatgacttt
     2461 gtgatgctgg cggattcttt ctatggcctt ggcttcaatc tggcggattc tttcgcgggt
     2521 gactccgaac tcctttccca cttcttccaa agtgtgtgaa atgccgtcgt ccaagccgaa
     2581 gcgcatccgc aaaattttat actccctcgg cataagatcc actaaaattt ctttgatatg
     2641 gtcttttaaa agttttctgg cggcttcctg cgccggagta agcatttgtt tgtcttcaat
     2701 aaaatctcca agcgttgaat cctcatcgtc ctcgcccacc ggcgcctcca aagaaacggt
     2761 gtcctgcgaa attttctgaa tgtggtgaat tttttccacc tctatattca tttccgcagc
     2821 caattcttcg gccaaaggat ccctgcccaa atcttgcgtc aagcggcgcc tgacctgctg
     2881 gtattttgaa atagtttcca ccatatgcac cggaatacgg atggtttttc cctggtcggc
     2941 aatggcgcgc gttacggcct ggcgaatcca ccaggtagcg taagttgaaa atttatagcc
     3001 cttgcgccag tcaaattttt ccaccgcccg aaaaagtccg agattcccct cttgaatcaa
     3061 atccagcatg gtgagatgcg gagtcctgcc cacatatttt ttagcaatgg aaacaaccaa
     3121 gcgtaggttg gcctgcgcca ggcggttttt ggcgtcttca tcgccggctt caatccggcg
     3181 ggcgagctcc ttttcctcgt ccgcggaaat taatggaatc cggccgattt ctttcaaata
     3241 catctgcaca gaatccgcgg ccgtgatgtc ttgatccaaa actaaagcgc gacggcctgc
     3301 ttttttcttg gctggctttt cttctttagc ttggacgctt tgaggaagtt ccaaaagctc
     3361 ccgcccttcc aaaacatcaa cacccgctga ttccaaacgg ccgtataaat cctccaaaaa
     3421 aacaacatta tactcaacat gcgggaaagc ggacaaaatt tccgggtaag ttaaaaaacc
     3481 gcgctctttc ccctttttaa gcaaaatctc aacgcgctct tcgctgaatt ctttttttag
     3541 ctcggctttg gagggtagtg aagcttttgc tctactaccc tctttggtaa tttttttcgc
     3601 tggagttttt ttagctggcg gttttttaac tgttttcttt gattttattt tctttttaat
     3661 ttttcgtttt ttaatcatag cgtgtgaagt tctttagaaa ttttttgaaa ttctcccaat
     3721 aaatctccga cgtttccttc gcctgttttt tcggcgctgc ttatttgggc gttcaaataa
     3781 gccagctttt cttttaaata ttcttttttg agctcccttt ggcattttat caattccgcc
     3841 tcggcgtcta ttatgccgct aagaaataat tccgcgaaaa ttgccgtccg cccgcctgcc
     3901 acgacggttc cgtcggagcc agaagaaatt aacgcgctaa gcgccgggtc aagcttgggt
     3961 accaacgccg gatattccgc gacaattccc aaaacttttt cttcgagcag ttcttttctg
     4021 gtttttggtt ttgacgctga attttcaaaa acattttccg ttccctccgc tggcttattt
     4081 ttttcggaag ccgaccagac agcttcctct tttatattta aaatcctgct tacttctctc
     4141 acccaatgcg cgcgctctaa atcagccaaa gaagatataa ccggcaagac agtccgctga
     4201 aattctcgct tggcttcagg agaattcggc ttgtgttttt ttagcgccga atctatgtaa
     4261 aattgcgcta tatgccgcga ggccgaaacc gctttttgcc aaagcgccgg atttttatag
     4321 actgcatccg ccgggtcctt aaggcccgcg tccaaagccc ctgcgactcg gacgtcgaat
     4381 ccgccagcca aagccaaatc aatgccgcgt ttggaagcgc cttcgccagc cgagtccata
     4441 tcaaaagcca caataagttt ttcggcaagc cgtcttattg ttttcaattg gtcgtgggtt
     4501 aaagccgttc cggaagcggc taccacgttt tttacgcccc cctgccatga cattatcacg
     4561 tccatataac cctcaactaa aacgcattcg gacgcgcgta aaatttcgct tttggcgaaa
     4621 tttagtccgt aaagaatttt gcttttttga taaagcgaag tttccggaga attaacatat
     4681 tttgcgactg cttcagtcgc tccgacagct tggctcgtcg gagcttccaa tattcttcct
     4741 ccaaaagcca caactctccc gctgtaatca aaaatcggaa acataatccg gccgcgaaac
     4801 cggtcgtaat aaccctgccc gctttttacc acgaggcccg aattttccaa atctttttcg
     4861 gaaaaatttt tttgcttgag atgcgcgccg agcgcgcgcc attcgtccga cgcgtacccc
     4921 aaacgaaaag tttttatggt ttcatcttgc aagccgcgct ttttgagata atccagtgcg
     4981 gttaaaccgc caagtggcgg tttaaccgcc aagttggatt caaaaaactt gcaagcttcc
     5041 tccaaaagcg cgaactgccg ggttcgttcg cttttcaaac gagggtcctc tctttttaat
     5101 tccactccgg cgcggtcggc cagagtttta agcgcgtcgc caaattcaac tccttcaatt
     5161 tgttggatga attcaaacat atcacctccc tttccgcagc cgaaacaatg ccaaatctgc
     5221 cgcgcgggag aaacattaaa tgaaggcgtc cgctcgttgt gaaatggaca aagcgcctta
     5281 aaattcacgc cagctttttg gagcctgata taggagccca ctagctccgc aatatccaag
     5341 cggtttttga tttcatcaat ttgagaagac ataatgtgga ataattatat catattactt
     5401 tttatttcaa aatatcccag atggccgggt cttctccgcc gtctattttg aatatcgcga
     5461 ttcctcgcac cccaagtttt ttggcaagat taattttatc ttttatggca accgcgtcgc
     5521 tccaccaaag taatttaagc gacgaagatt ctgttgtggt ggatatgtac ataaagctta
     5581 attctccagc tatgtttctt tgaggaaaaa cccctaagtc cttggctaaa tccaaagcgt
     5641 atctcggatt aaacgccgaa attaaattat atctaaaacc ttcttccgca agaggtttaa
     5701 cgtcaaattc atagccgtaa gtcgccacgc ctataataag cttctttttt gaaatatttt
     5761 ttgcggcaag attcactact ttttcaaccc acttcgcatc ggcaatcgga atataggggc
     5821 ccaaagccga tttgttcaag cgcaaatcta tcgtaccctg atcgtaagtc atcaacctca
     5881 cgcggtcgca atatttattg atcgcgacaa aatcattggc gtatcggata tctttgggaa
     5941 tagtgtcata cctagaatca agcggtgtgc gcgcctcaat agtgcacatc acccactttt
     6001 ttcccatgcg ctggtaaagg cctttaagga acgttgaaaa ataatttttc gtttccgctt
     6061 ttttgccttc aaaatcaata tcaatgccgt caaacccttt ttctttaacc aacaaggcaa
     6121 tctcgtcctc aagagcaatg cgtgtttttt gatttgaaag aatacggtga attgcttcgg
     6181 tgttgctcca cattacggtg ggaataatgc gaatctttcg tttttttgct tcggcgataa
     6241 gcgtcgtcca cggctcctcg tctattttca tagcgtcaaa aagcgtccct tcatttttga
     6301 ccgtgtagcc gaatggattt atttctttaa aagaatcaat gtgcgccaaa gcgtcggccg
     6361 tccccgtggc cgtccgccaa tatggaatcc agccggatat ttccagaggc ggcgtggcgg
     6421 ttttcgcgta ggcgcccgaa ggcgccaaaa tcaaaaacac agcaagcgcg aattttaaaa
     6481 tcatttgttg tataattttt gaatttttaa aaaaaatatc atcagcaaaa atgaagtaag
     6541 agacattata ggaatagtga tatatcccag ctcaaaaaca taacgtttag cgcaagagac
     6601 gcttaactgg agagcatcgc aattaaaaaa cggcgagccc ccgaaagaga gataattatg
     6661 gtaaagcgaa acaacggttc cgacaaaaag cagagccaaa gcgtaatcaa taattttata
     6721 atcttttcta taaagcgcca tccccaaaag aattacttga gggtaaataa aaatccgctg
     6781 aagccagcaa agcttgcagg gttcatatcc ggcgtagtcc gaataaaaaa ggctcaagat
     6841 tagggaagcc gttgccgcca gaaatgctaa aagcattcca tgccgtccga aaaaatttaa
     6901 aatataattt cttgttttgg ggaaaaacat taaaaccaac gcccacacca aaaaaatttg
     6961 ggcgaaaagc gtgaatatcg cggagatttt atttacatat tccaaaagca tattatttgg
     7021 gcaaggggct cacttcgctt tcgctttggc gaagcgggca gcttgttttt ttagacaatg
     7081 tttctagcgg cacttctccg ctttccctgc ttccatcggc aaactcccat gttggatagc
     7141 cggatatttt tttatcgttg cacaagggca attggctccg gccatccggc gttgagcatt
     7201 ccacatatgg caaaaatttc tcggaacttc caaacatctt tttttggttc tggcagtgag
     7261 gacaccaaaa agcgccgtaa aaaatcgcgc ccttgtcttt caagcatttg gtgaaatcat
     7321 cgtatttgga ggggctgcta tcggctttca ttaaaaacca agccccgccg acgattatcg
     7381 ccgcccccgc ggcaatccag atcgcgatat tttttatttg catgctacta aaataactct
     7441 tttgttatgt aatatcaaat atcccgcctc gtccgttgtg atacgaacat tttccgcaat
     7501 cctcgttatg ccccggatat gatttgttta agagcttcgc catatccgcg agtttttgtt
     7561 caatcgcgcc taaatcaatt ttcacgaggt ccacgtggct cgcgaacttc acttcgattg
     7621 atggattgct tttgtctttc acaaaataat gcagaagata tgccctgtca gaaactttgc
     7681 gcccgtgttt tttaaacata aatccgtagg ccgctaattg gtcgcggtaa tatttttgtt
     7741 tgtcttcctt cggcgcgttg ccggaagatt tataatccgc cggtatcaaa cggccgtcgg
     7801 cctccaccag cacatcgtca attttgcccg tgagttcata accagcgcct tcgtcaacca
     7861 ctttcagcgc ccccgtcttc gcccgccatt cgttaagcgt atcaatatct tcaaatggct
     7921 tgatgccctc tttttcaagc tctttcgctt cgggcgggaa cgcgccatcc gcccggtatt
     7981 tgtcatacct ggctttcaaa atgctgtcca tcgccgaatt taaaagcaac ggcaacatcg
     8041 gcgctttttt ataatgattg tccgcccaaa aacagcttcg gcactcggtg tacccgtaaa
     8101 gtccgctcgg gcttaatttc cataatcctt cttttgtttt cattaattaa attattctaa
     8161 catatcaatt taaaaagcgt tcgctgggga cttgtcagta gtattcccgc tgaaagtcac
     8221 gttcgtcgcc tgaatcgttg aatttgcgga aataaaagcg gtggaattac cggtcggagc
     8281 agtatgatta ttaaattcaa agttagtgag cgtggaagtg gcgcggtcat aaagccgcat
     8341 tccggcaaga taattatttt caatccgccc gtccgataaa ttaacgctcc cgccaaaaac
     8401 tccgatgccc gctatcgcgt tggggttgcc tccatcgcct cttccgccgt agcgcaaagt
     8461 aaaacccccg ccgtaaagat atcctccggc gttcacctcc attccgtacc actgtgccgg
     8521 agcggaatcc gccacggaag tgaaaattat gctatctttg ttcgcgcctt caagttttaa
     8581 agcgccgtta accacaaatc gagaataagg cgtaagtcct ttccaaacag cgcctgtctc
     8641 aataatcgcc gcggatccag ccgcgacttt tggattttcg taaggatatt cgccgattaa
     8701 ataaggaatg gcgttagcgt aaattgtagt agtgccggag gccgtagaaa ttgttcccga
     8761 aagcgcgatg ccatttttgc cgtttcccgc gccggaattt ccagaaaaag tactggcgcc
     8821 gtttatagaa taagccgcgt aagaaatatt gttggtaaaa acattatccg aaacggacgc
     8881 ggacccgccc tccgctttaa atccgtaata attttccaaa aaagtatttc cggaaactgt
     8941 cggcgagccg ccgatgatgt aaaggcctac gttgtccgtc gttgttgtgc caacggagaa
     9001 aatgctgttt tgaaccgtgg aattggaagt tattaatctc attcccacag aaaaggaact
     9061 ctcaaaaatt gaattggaaa tcgttacgga attatcaatt acgctgaaaa gcgccctgcg
     9121 ttccaaagga atattgctgg taaatccgcc gccgtattta aaacgggtat aactcacgct
     9181 ggaattttga ctgcttgagg taatccgcac atttctccaa ctgcccgaga atggcggcga
     9241 ggataagctg gtaaataaaa tattgttctc cgcagtgccg tcggatttta gagtgccgtt
     9301 cacaatcaaa tccgccgtgt cgcccatttt taccgtaact cccggttcaa tcgtcaaggt
     9361 ttttccagcg ggcaccgtaa aattctcgct tggccttatg atgtaagtgc cccaagattt
     9421 tttgagaacg cggtcagccg ataatgtgga attttgggaa attaaataat gcaaagaatt
     9481 cctggcgcgc ggagtcgcgg taagatttgc tccaaccgcg tcttttccgt ttttaataaa
     9541 actgttggag gcgccggaat tggaagtgct ccagtttgtt gtatccgtgc cagcgatatc
     9601 ggggtcaatt ctttccattg tggaataaac tccgcccgcg gcgcccccgc accatcttcc
     9661 gggacaaaga gcggtttggt caatggtagt tgaagcttta gaaagaatta aaatttctcc
     9721 tgaatttcca agcccggagc cagagctcaa gccagagaaa ggcacaatca aatcagccgc
     9781 gacatcactt acagtattat cattcgtcct ttctattaaa taatatcctc ttgctgaaac
     9841 agcccccaaa agattaatgt atggggcgcc gtcgctcgcg tataaaaccc aattcgcgag
     9901 attcacgctt tgcgaacttc tattataaag ttcaatccat tcgtcgttcg cgaaaggcgc
     9961 ggacgtcccg gcccacgcca cttcgttaat gactaccgga cgcagggaga gctccgcggt
    10021 ttttgtcgtc tgcgcgcttt cgttgccggc gccgtcaacc gctttggctt tgaaaatata
    10081 aattgagtcg tctgtcggca aagaataaat agtagaagtg gcggtggtgc tcgcaaaatt
    10141 aaatccagag catccgccaa ctggcggact ggatttttca cactcaatta tataatgatt
    10201 tagatcgttc gccgttgaag accaaactaa agaaaccgtg gacgacgcga gcaggcatcc
    10261 gtctgaagaa agcgagctag aacattcgct caccgaaagc gaaatatcgg gcggggttgt
    10321 gtccggcggc tccgaagttt cagtcccgcc aagcgactga acctcggagg acgtcgtgag
    10381 aatttgaaca gatggagttg gcgatccgcc accgccaaag ccagccgagc ccccgccaat
    10441 ggcttgcagt tgcagaaagg gcggcaaaga cccactttcc gtattcccac ccacttgcga
    10501 cattccaaca ttctcaagaa tgttggaatg attttcctcc tcaactccgc ccggagaaac
    10561 accgagtgtt gaggagcgag atatttcact tagacgtccg acgtctaagt ccgattttgc
    10621 gcccacaatc tcttcacttg gcggtttaac cgccaagttg atttggaaag cattgtcggg
    10681 aatttccgaa accagaggtg tcggctccgg ctggcttat
//
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