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Salmonella enterica subsp. enterica serovar Hissar strain SCPM-O-B-4549 plasmid pB-4549_2, complete sequence

GenBank: CP088140.1

FASTA Graphics 

LOCUS       CP088140                8274 bp    DNA     circular BCT 23-MAY-2022
DEFINITION  Salmonella enterica subsp. enterica serovar Hissar strain
            SCPM-O-B-4549 plasmid pB-4549_2, complete sequence.
ACCESSION   CP088140
VERSION     CP088140.1
DBLINK      BioProject: PRJNA269675
            BioSample: SAMN23378276
KEYWORDS    .
SOURCE      Salmonella enterica subsp. enterica serovar Hissar
  ORGANISM  Salmonella enterica subsp. enterica serovar Hissar
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 8274)
  AUTHORS   Bogun,A., Kislichkina,A., Mukhina,T., Solomentsev,V., Skryabin,Y.,
            Shishkina,L., Sizova,A., Galkina,E. and Kurilova,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-NOV-2021) Science Department, SRCAMB, Building 1,
            Obolensk, Moscow region 142279, Russia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Unicycler v. 0.4.7
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 320.75x
            Sequencing Technology  :: Oxford Nanopore MinION; MGISeq-2000
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 11/30/2021 17:07:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 5.3
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 4,945
            CDSs (total)                      :: 4,821
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 59 of 135
            Pseudo Genes (incomplete)         :: 81 of 135
            Pseudo Genes (internal stop)      :: 31 of 135
            Pseudo Genes (multiple problems)  :: 32 of 135
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8274
                     /organism="Salmonella enterica subsp. enterica serovar
                     Hissar"
                     /mol_type="genomic DNA"
                     /strain="SCPM-O-B-4549"
                     /serovar="Hissar"
                     /sub_species="enterica"
                     /db_xref="taxon:2564534"
                     /plasmid="pB-4549_2"
     gene            complement(join(8222..8274,1..271))
                     /locus_tag="LRM92_24640"
     CDS             complement(join(8222..8274,1..271))
                     /locus_tag="LRM92_24640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="UQY59716.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYADVLIAITEYHRALLS
                     RLMAD"
     gene            556..705
                     /locus_tag="LRM92_24645"
     CDS             556..705
                     /locus_tag="LRM92_24645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UQY59717.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHEL
                     AEEGV"
     gene            complement(844..1704)
                     /locus_tag="LRM92_24650"
     CDS             complement(844..1704)
                     /locus_tag="LRM92_24650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="UQY59718.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            complement(1887..2444)
                     /locus_tag="LRM92_24655"
     CDS             complement(1887..2444)
                     /locus_tag="LRM92_24655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="UQY59719.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTD
                     REGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGD
                     MGQMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTG
                     ATEIAHQLSIARSTVYKILEDERAS"
     gene            2608..5613
                     /locus_tag="LRM92_24660"
     CDS             2608..5613
                     /locus_tag="LRM92_24660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /protein_id="UQY59720.1"
                     /translation="MPVDFLTTEQTESYGRFTGEPDELQLARYFHLDEADKEFIGKSR
                     GDHNRLGIALQIGCVRFLGTFLTDMNHIPSGVRHFTARQLGIRDITVLAEYGQRENTR
                     REHAALIRQHYQYREFAWPWTFRLTRLLYTRSWISNERPGLLFDLATGWLMQHRIILP
                     GATTLTRLISEVREKATLRLWNKLALIPSAEQRSQLEMLLGPTDCSRLSLLESLKKGP
                     VTISGPAFNEAIERWKTLNDFGLHAENLSTLPAVRLKNLARYAGMTSVFNIARMSPQK
                     RMAVLVAFVLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDKSALALA
                     SACSYLLKEETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHEEMVEQYGRV
                     RRFLPHLLNTVKFSSAPAGVTTLNACDYLSREFSSRRQFFDDAPTEIISRSWKRLVIN
                     KEKHITRRGYTLCFLSKLQDSLRRRDVYVTGSNRWGDPRARLLQGADWQANRIKVYRS
                     LGHPTDPQEAIKSLGHQLDSRYRQVAARLCENEAVELDVSGPKPRLTISPLASLDEPD
                     SLKRLSKMISDLLPPVDLTELLLEINAHTGFADEFFHASEASARVDDLPVSISAVLMA
                     EACNIGLEPLIRSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPLAQIWGG
                     GEVASADGMRFVTPVRTINAGPNRKYFGNNRGITWYNFVSDQYSGFHGIVIPGTLRDS
                     IFVLEGLLEQETGLNPTEIMTDTAGASELVFGLFWLLGYQFSPRLADAGASVFWRMDH
                     DADYGVLNDIARGQSDPRKIVLQWDEMIRTAGSLKLGKVQVSVLVRSLLKSERPSGLT
                     QAIIEVGRINKTLYLLNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYT
                     DGQEDQLGTLGLVTNAVVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINML
                     GHYSFTLAELVTKGHLRPLKEASEAENVA"
     gene            6192..6305
                     /locus_tag="LRM92_24665"
     CDS             6192..6305
                     /locus_tag="LRM92_24665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UQY59713.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLSPLAAAIGN"
     gene            6570..7610
                     /gene="lsoA"
                     /locus_tag="LRM92_24670"
     CDS             6570..7610
                     /gene="lsoA"
                     /locus_tag="LRM92_24670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA
                     endoribonuclease LsoA"
                     /protein_id="UQY59714.1"
                     /translation="MAQNPFKALNINIDKIESALTQNGVTNYSSNVKNERETHISGTY
                     KGIDFLIKLMPSGGNTTIGRASGQNNTYFDEIALIIKENCLYSDTKNFEYTIPKFSDD
                     DRANLFEFLSEEGITITEDNNNDPNCKHQYIMTTSNGDRVRAKIYKRGSIQFQGKYLQ
                     IASLINDFMCSILNMKEIVEQKNKEFNVDIKKETIESELHSKLPKSIDKIHEDIKKQL
                     SCSLIMKKIDVEMEDYSTYCFSALRAIEGFIYQILNDVCNPSSSKNLGEYFTENKPKY
                     IIREIHQETINGEIAEVLCECYTYWHENRHGLFHMKPGIADTKTINKLESIAIIDTVC
                     QLIDGGVARLKL"
     gene            7607..7987
                     /gene="lsoB"
                     /locus_tag="LRM92_24675"
     CDS             7607..7987
                     /gene="lsoB"
                     /locus_tag="LRM92_24675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin LsoB"
                     /protein_id="UQY59715.1"
                     /translation="MKKDKKYQIEAIKNKDKTLFIVYATDIYSPSEFFSKIESDLKKK
                     KSKGDVFFDLIIPNGGKKDRYVYTSFNGEKFSSYTLNKVTKTDEYNDLSELSASFFKK
                     NFDKINVNLLSKATSFALKKGIPI"
ORIGIN      
        1 tcagcacgtc cgcatactcc ctgtccccga cacgcttgcc gtcgataaag agttttttct
       61 gcaacgcccc cagtcgcata acttctttca gcacccggtc atcagtcagt gagttaactt
      121 tcttaccgag agctgccgcc cgtaaaaaac cggaaacggt cttgccgcat tcagcagccc
      181 tttttctgat ttcctggtct tcttctgccg tcagacgaac agggcgactg atagcccgcc
      241 tgcgcgtatt acttccactt cgttttgtca taaaacatgg ctccgtatct gacatggggt
      301 gtcggggcga agccctgacc agggtaattg taatagcgcg catgtatgcg cggtataaca
      361 attacacatc ctgtcctgtc agcaagttcg aattaccgac aaaggcacgg cagttttttg
      421 gcagtgacag tattgtcagg cagaaatgcc cgtcaaagag tcagacagca gaatactggc
      481 ggcaaaactg tcgaaaagtg gtacgcgata taatcacata acgcagatga cagacagaac
      541 ggagaaaaca gaaagatgaa caagcagcag caaactgtac tcaacatggc gggattcata
      601 aaaagccaga gcctgacgct gctcgaaaag ctggacgcac tcgatgctga cgaacaggct
      661 gccatgtgtg agaagctgca cgaactcgca gaagaagggg tctgacgctc agtggaacga
      721 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
      781 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
      841 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
      901 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
      961 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     1021 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     1081 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     1141 caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     1201 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     1261 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggcag cagtgttatc
     1321 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     1381 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     1441 ttgctcttgc ccggcgtcaa cacgggataa taccgcacca catagcagaa ctttaaaagt
     1501 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     1561 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     1621 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     1681 gacacgaaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttacca
     1741 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     1801 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
     1861 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcaag aattttataa
     1921 accgtggagc gggcaatact gagctgatga gcaatttccg ttgcaccagt gcccttctga
     1981 tgaagcgtca gcacgacgtt cctgtccacg gtacgcctgc ggccaaattt gattcctttc
     2041 agctttgctt cctgtcggcc ctcattcgtg cgttctagga tcctccggcg ttcagcctgt
     2101 gccacagccg acaggatggt gaccaccatt tgccccatat caccgtcggt actgatcccg
     2161 tcatcaatga accggactgc cacgccctga gcgtcaaatt cctttatcag ttggatcata
     2221 tcggcagtgt cgcggccaag acggtcgagc ttcttaacca gaatgacatc accttcctcc
     2281 accttcatcc tcagcaaatc cagcccttcc cggtctgttg aactgccgga tgccttatcg
     2341 gtaaatatac ggtttgcttt cacacctgcg tctttgagtg ctctgacctg aagatcaaga
     2401 gactgctgac tggttgagac ccgagcgtaa ccaaaaagtc gcataaaaat gtaccttaaa
     2461 tcgaatatcg gacaactcat gtctattatt acaaatttac gatttaatag acatattaat
     2521 gtaacagttt tacgatgtcc gataatttat aacatttcgt acggttggaa aaatgttact
     2581 aaatgcccgt caggcaggga ggccgatatg cccgttgact ttctgaccac tgagcagact
     2641 gaaagctatg gcagattcac cggtgaaccg gatgagcttc agctggcacg atattttcac
     2701 cttgatgaag cagacaagga atttatcgga aaaagcagag gtgatcacaa ccgtctgggc
     2761 attgccctgc aaattggatg tgtccgtttt ctgggcacct tcctcaccga tatgaatcat
     2821 attccttccg gcgtccggca ttttaccgcc agacagctcg ggattcgtga tatcaccgtt
     2881 cttgcagaat acggtcagag ggaaaatacc cgccgtgagc atgcagcgct gatacgtcag
     2941 cactatcagt atcgtgaatt tgcctggccc tggacatttc gccttacccg tcttttatat
     3001 acccggagct ggataagcaa cgaacgtcct ggcctgcttt tcgatctggc gacagggtgg
     3061 cttatgcaac atcgtattat tctccccgga gccactacgc tgacccggtt gatttcagag
     3121 gtaagggaaa aggcgacgtt gcgcctgtgg aacaaactgg cactgatacc gtcagccgaa
     3181 cagcgttcac agctggagat gctgctgggg ccaactgatt gcagccgcct gtctttactg
     3241 gaatcactga aaaagggccc tgtgaccatc agtggtccgg cgtttaatga agcaattgaa
     3301 cgctggaaaa ctctgaacga ttttggcctg catgctgaaa acctgagtac actcccggct
     3361 gtgcgcctga aaaatctcgc acgttatgct ggtatgactt cggtgttcaa tattgccagg
     3421 atgtcaccgc agaaaaggat ggcggttctg gttgcctttg tccttgcatg ggaaacgctg
     3481 gcgctggatg atgcattgga cgttctggac gccatgctgg ccgttatcat ccgtgacgcc
     3541 agaaagattg ggcagaaaaa acggctccgc tcgctgaagg atctggataa atctgcattg
     3601 gcgctcgcca gcgcatgttc gtacctgctg aaagaagaaa caccggacga atcgattcgt
     3661 gctgaggtgt tcagctacat cccaaggcaa aagctggctg aaatcatcac gcttgtccgt
     3721 gaaattgccc ggccctcaga cgataatttt catgaagaaa tggtggagca gtacgggcgc
     3781 gttcgtcgtt tcctgcccca tctgctgaat accgttaaat tttcatccgc acctgccggg
     3841 gttaccactc tgaatgcctg tgactacctc agccgggagt tcagctcacg gcggcagttt
     3901 tttgacgacg caccaacgga aattatcagt cggtcatgga aacggctggt gattaacaag
     3961 gaaaaacata tcacccgcag gggatacacg ctctgctttc tcagtaaact gcaggatagt
     4021 ctgaggcgga gggatgtcta cgttaccggc agtaaccggt ggggagatcc tcgtgcaaga
     4081 ttactacagg gtgctgactg gcaggcaaac cggattaagg tttatcgttc tttggggcac
     4141 ccgacagacc cgcaggaagc aataaaatct ctgggtcatc agcttgatag tcgttacaga
     4201 caggttgctg cacgtctttg cgaaaatgag gctgtcgaac tcgatgtttc tggcccgaag
     4261 ccccggttga caatttctcc cctcgccagt cttgatgagc cggacagtct gaaacgactg
     4321 agcaaaatga tcagtgatct actccctccg gtggatttaa cggagttgct gctcgaaatt
     4381 aacgcccata ccggatttgc tgatgagttt ttccatgcta gtgaagccag tgccagagtt
     4441 gatgatctgc ccgtcagcat cagcgccgtg ctgatggctg aagcctgcaa tatcggtctg
     4501 gaaccactga tcagatcaaa tgttcctgca ctgacccgac accggctgaa ctggacaaaa
     4561 gcgaactatc tgcgggctga aactatcacc agcgctaatg ccagactggt tgattttcag
     4621 gcaacgctgc cactggcaca gatatggggt ggaggagaag tggcatctgc agatggaatg
     4681 cgctttgtta cgccagtcag aacaatcaat gccggaccga accgcaaata ctttggtaat
     4741 aacagaggga tcacctggta caactttgtg tccgatcagt attccggctt tcatggcatc
     4801 gttataccgg ggacgctgag ggactctatc tttgtgctgg aaggtcttct ggaacaggag
     4861 accgggctga atccaaccga aattatgacc gatacagcag gtgccagcga acttgtcttt
     4921 ggccttttct ggctgctggg ataccagttt tctccacgcc tggctgatgc cggtgcttcg
     4981 gttttctggc gaatggacca tgatgccgac tatggcgtgc tgaatgatat tgccagaggg
     5041 caatcagatc cccgaaaaat agtccttcag tgggacgaaa tgatccggac cgctggctcc
     5101 ctgaagctgg gcaaagtaca ggtttcagtg ctggtccgtt cattgctgaa aagtgaacgt
     5161 ccttccggac tgactcaggc aatcattgaa gtggggcgca tcaacaaaac gctgtatctg
     5221 cttaattata ttgatgatga agattaccgc cggcgcattc tgacccagct taatcgggga
     5281 gaaagtcgcc atgccgttgc cagagccatc tgtcacggtc aaaaaggtga gataagaaaa
     5341 cgatataccg acggtcagga agatcaactg ggcacactgg ggctggtcac taacgccgtc
     5401 gtgttatgga acactattta tatgcaggca gccctggatc atctccgggc gcagggtgaa
     5461 acactgaatg atgaagatat cgcacgcctc tccccgcttt gccacggaca tatcaatatg
     5521 ctcggccatt attccttcac gctggcagaa ctggtgacca aaggacatct gagaccatta
     5581 aaagaggcgt cagaggcaga aaacgttgct taacgtgagt tttcgttcca ctgagcgtca
     5641 gaccccaaga actccagaac agcatccaga cacgctttga agtagaaaat agcacagaga
     5701 gataactctc aggacaggat gccgaacggc acaacagagc gtaaggagaa aatgtcacat
     5761 aaggcgctct tccgcttcct cgctcactga ctcgctacgc tcggtcgttc gactgcggcg
     5821 agcggtgtca gctcactcaa aagcggtaat acggttatcc acagaatcag gggataaagc
     5881 cggaaagaac atgtgagcaa aaagcaaagc accggaagaa gccaacgccg caggcgtttt
     5941 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagc cagaggtggc
     6001 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     6061 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     6121 tggcgctttc tcatagctca cgctgttggt atctcagttc ggtgtaggtc gttcgctcca
     6181 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     6241 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccattggta
     6301 actgatttag aggagtttgt cttgaagtta tgcacctgtt aaggctaaac tgaaagaaca
     6361 gattttggtg actgcgctcc tccaagccag ttaccttggt tcaaagagtt ggtagctcag
     6421 cgaaccttga gaaaaccacc gttggtagcg gtggtttttc tttatttatg agatgatgaa
     6481 tcaatcggtc tatcaagtca acgaacagct attccgttga caaaaaaaca agatctgtac
     6541 atattcaaaa aaaacaacgg attgacccaa tggcacagaa cccgtttaaa gcactaaata
     6601 tcaatattga caagattgag tctgctctga cgcagaatgg cgtcacaaac tattcctcta
     6661 atgtaaaaaa cgaaagagaa actcacatat ctggcacata taaaggaata gacttcttaa
     6721 taaaactaat gccatcaggc ggaaatacca ctatcggaag agcgtctggg caaaataaca
     6781 cttactttga tgaaatcgcc ttgattataa aagaaaactg tttgtattca gacacaaaga
     6841 actttgaata caccattcca aaattcagtg atgatgacag ggcaaatcta tttgaatttc
     6901 tttctgaaga ggggataaca ataacagaag ataataataa cgatcctaat tgtaaacacc
     6961 aatatattat gaccaccagc aatggtgaca gggtcagggc aaaaatttac aagcgcggct
     7021 ctattcaatt ccaaggaaaa taccttcaaa tcgcgagttt gattaacgat ttcatgtgct
     7081 caatactaaa catgaaagag attgtcgaac aaaaaaataa agaatttaat gttgacataa
     7141 aaaaagaaac tattgagtcc gagttgcata gcaaactacc aaaaagcatc gataaaatcc
     7201 atgaagatat caaaaaacag ctatcatgct cgctaataat gaaaaaaata gatgtcgaaa
     7261 tggaagatta ctcaacatac tgcttctctg cattaagagc catagaaggc tttatatatc
     7321 aaatacttaa tgatgtttgc aatccatcat catcaaagaa ccttggcgaa tacttcactg
     7381 aaaacaaacc caaatatata atcagagaaa tacaccaaga aactataaat ggtgaaatag
     7441 cggaagtttt gtgtgaatgc tacacttact ggcatgagaa caggcatggt ttgtttcata
     7501 tgaaaccagg aatagctgac acgaagacaa ttaacaaatt agaatcaatc gcaatcatcg
     7561 ataccgtttg ccaattaata gatggtggcg tagctaggtt gaaattatga aaaaagataa
     7621 aaaatatcaa atagaagcaa taaaaaataa agataaaact ttatttattg tctatgctac
     7681 tgatatttat agcccgagcg aatttttctc aaaaatcgaa tccgacttga agaaaaagaa
     7741 aagcaagggt gatgtttttt ttgatttaat aattcctaac ggtggaaaaa aagatcgtta
     7801 cgtctatacg tcatttaatg gcgagaagtt ttcaagttac acattaaaca aagttacgaa
     7861 aactgatgaa tataatgatt tatctgagct ctcggcttcg ttctttaaaa aaaactttga
     7921 taagatcaac gtaaaccttc tatccaaagc cacatcattt gctttgaaaa aaggcattcc
     7981 aatataatat caagccagaa gacatttacc ataacgtctc attacgcgca ccgccaaaac
     8041 gcgctgaacg cgattctgac gcagacaccg caaaaagaga tgttttcccc ggggtaaacc
     8101 ggaaaaatgc gtcaggatcg ctttcagctc actgcatagc tatgcatgaa agtgaatggc
     8161 gatcggtttg ggggcttacg gcgttcatac cgtctgtttt cgacagtttc tctccgggaa
     8221 gctaatctgc cataagcctg gataacaggg cacggtgata ctccgtaata gcga
//
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