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AGENCOURT_15764347 NIH_MGC_204 Mus musculus cDNA clone IMAGE:30525111 5', mRNA sequence

GenBank: CF617643.1

FASTA Graphics 

LOCUS       CF617643                 739 bp    mRNA    linear   EST 19-DEC-2010
DEFINITION  AGENCOURT_15764347 NIH_MGC_204 Mus musculus cDNA clone
            IMAGE:30525111 5', mRNA sequence.
ACCESSION   CF617643
VERSION     CF617643.1
DBLINK      BioSample: SAMN00173818
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 739)
  CONSRTM   National Institutes of Health, Mammalian Gene Collection (MGC)
  TITLE     NIH-MGC EST Sequencing Project
  JOURNAL   Unpublished
  REMARK    http://mgc.nci.nih.gov/
COMMENT     Contact: Daniela S. Gerhard, Ph.D.
            Office of Cancer Genomics
            National Cancer Institute / NIH
            Bldg. 31 Rm10A07 Bethesda, MD 20892
            Email: cgapbs-r@mail.nih.gov
            Tissue Procurement: Naryan Bhat
             cDNA Library Preparation: Express Genomics
             cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
             DNA Sequencing by: Agencourt Bioscience Corporation
             Clone distribution: MGC clone distribution information can be
            found through the I.M.A.G.E. Consortium/LLNL at:
            http://image.llnl.gov
            
            Plate: NDAM605  row: l  column: 16
            High quality sequence stop: 713.
FEATURES             Location/Qualifiers
     source          1..739
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /clone="IMAGE:30525111"
                     /clone_lib="SAMN00173818 NIH_MGC_204"
                     /lab_host="DH10B (phage-resistant)"
                     /note="Organ: placenta; Vector: pExpress-1; Site_1: EcoRV;
                     Site_2: NotI; RNA obtained from three placentas from
                     female C57/BL6 mouse at 16 days pregnancy. Tissues were
                     snap-frozen and kept at -80C for two days before RNA
                     extraction and purification (Tri-reagent method). cDNA was
                     primed using oligo-dT primer:
                     5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into
                     the EcoRV/NotI sites of pExpress-1. Size-selection >0.75kb
                     resulted in an average insert size of 1.1 kb. This
                     primary, nanoquantity library is normalized to Cot5
                     (non-normalized primary library is NIH_MGC_223) and was
                     constructed by Express Genomics (Frederick, MD)."
ORIGIN      
        1 cgaaatggct tcgaaaatcg tgatgtcttc gaaaaccgtg aagacctccg acgagatcct
       61 ttgcgagggc gagctggaga agcgaagcga cagcctgttc caggtatgga agaagaagcg
      121 ctgcgtgctc accgccgacc gcctgcgcct cttctccggg aaaaccagcc ccgccaagga
      181 gctgtttttc cactccatcc tcaaggtgga ctgcgtggag cacacctcta agtacgtgta
      241 cttcaccatc gtcaccaact attacaagga gatcgacttc cgctgcacgg tggagagctg
      301 ctggaacgcg gccatcacca tggcgctgat cgacttccag aaccgtcggg cgctgcaaga
      361 ctttccccgc taccggtatc agcgctctga gtctgaaatg ccttccgagc cgggagagca
      421 gagcgctctg ggtccgtgaa acgcacggga ccagttgagc cgggaaccag cccacgctgc
      481 cgcatcagac aggagcccag gagcccaggg gatgccccgg gtgacgccgc cgctgctctt
      541 caccgaagat attcccgtgc ttgctgagcc ttgccccgct gtgcgggtgc gctaacttat
      601 tggaccgtat ttatattggt tacctgcttc caacccacca ttccgtgtta atatttttta
      661 taccatattt tcattccaaa taaacaatgt cacttttctt tttttaacaa aaaaaaaaaa
      721 aaaaaaaaaa aaagggcgg
//
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