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GenBank: CB325077.1
FASTA Graphics
LOCUS CB325077 748 bp mRNA linear EST 27-JAN-2011 DEFINITION UI-R-DZ0-crm-b-12-0-UI.s1 NCI_CGAP_DZ0 Rattus norvegicus cDNA clone IMAGE:7341710 3', mRNA sequence. ACCESSION CB325077 VERSION CB325077.1 DBLINK BioSample: SAMN00170072 KEYWORDS EST. SOURCE Rattus norvegicus (Norway rat) ORGANISM Rattus norvegicus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus. REFERENCE 1 (bases 1 to 748) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) PUBMED 8889548 COMMENT Contact: Daniela S. Gerhard, Ph.D. Office of Cancer Genomics National Cancer Institute / NIH Bldg. 31 Rm10A07 Bethesda, MD 20892 Email: cgapbs-r@mail.nih.gov Oligo-dT track not found, Not I site shown in beginning of sequence is likely internal to the message. cDNA Library Preparation: M.B. Soares Lab Clone distribution: clones will be available through IMAGE (http://image.llnl.gov) Seq primer: M13 FORWARD POLYA=Yes. FEATURES Location/Qualifiers source 1..748 /organism="Rattus norvegicus" /mol_type="mRNA" /strain="Sprague-Dawley" /db_xref="taxon:10116" /clone="IMAGE:7341710" /clone_lib="SAMN00170072 NCI_CGAP_DZ0" /dev_stage="adult" /lab_host="DH10B (Life Technologies)" /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI; UI-R-DZ0 is a non-normalized Rat cartilaginous tumor library (RCT) constructed in pT3T7 PAC vector according to the procedure described by Bonaldo, Lennon & Soares (Genome Research Genome 6: 791-806, 1996). The oligonucleotide used to prime first strand synthesis contained the sequence tag CATTCTTGTA between the Not I cloning site and dT18 stretch. The Rat cartilaginous tumor tissue was provided by Dr Jeff Stevens at the University of Iowa. TAG_LIB=UI-R-DZ0; TAG_SEQ=CATTCTTGTA; TAG_TISSUE=rat SRC-JWST tumor line" ORIGIN 1 tttttttttt ttttttttct ttctttcctt tccgagcctt ttgaggctga ggtctatttg 61 tcagctgata tcccaactat ttaagactaa cagttaaaat aacagtcagt gtatgagaga 121 gttaatgtgc ttggccacta ctaaggatga accagctagg gcttcttcaa gtcctcagct 181 caccgcggtc cgttggccct gatcagttgg cttgtcctgc aatatggttt tcgctgtcac 241 tcccatagtc gacgtcgggg tcctcctcct cctcctcgta ctcctcataa atctctccgt 301 tgatgtcctc agcctggatt tcttccttga tgtgaggctc ctctcccttc acgccgtaag 361 tgctctgaat cacgggcatc ccgggctctg ggctgctgga cacgctcgag tgctctgagg 421 gcaggtgagg ggacggcatt cctgccatgg cgatggcacc ggggtacacg acaccggccg 481 tagcgatggg gatctgtgct tgttgcccaa cgttgaagta ctgtcgcatt tcctgcctcc 541 gattccgcat gatggccttg tactctccaa tgcgcagttt cttcncatct accagacaag 601 tgcgcttcgg cctgngtttg tacttgtagt ctgggtactt ctccaggtgc tgtttgctga 661 gcccgggctg ctcctcatag tatggctgtt tctctangtt ggtcatagct ttccaacgag 721 atcccagtat cttgctgatn gttggatg //
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