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UI-R-DZ0-crm-b-12-0-UI.s1 NCI_CGAP_DZ0 Rattus norvegicus cDNA clone IMAGE:7341710 3', mRNA sequence

GenBank: CB325077.1

FASTA Graphics 

LOCUS       CB325077                 748 bp    mRNA    linear   EST 27-JAN-2011
DEFINITION  UI-R-DZ0-crm-b-12-0-UI.s1 NCI_CGAP_DZ0 Rattus norvegicus cDNA clone
            IMAGE:7341710 3', mRNA sequence.
ACCESSION   CB325077
VERSION     CB325077.1
DBLINK      BioSample: SAMN00170072
KEYWORDS    EST.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Bonaldo,M.F., Lennon,G. and Soares,M.B.
  TITLE     Normalization and subtraction: two approaches to facilitate gene
            discovery
  JOURNAL   Genome Res. 6 (9), 791-806 (1996)
   PUBMED   8889548
COMMENT     Contact: Daniela S. Gerhard, Ph.D.
            Office of Cancer Genomics
            National Cancer Institute / NIH
            Bldg. 31 Rm10A07 Bethesda, MD 20892
            Email: cgapbs-r@mail.nih.gov
            Oligo-dT track not found, Not I site shown in beginning of sequence
            is likely internal to the message. cDNA Library Preparation: M.B.
            Soares Lab Clone distribution: clones will be available through
            IMAGE (http://image.llnl.gov)
            Seq primer: M13 FORWARD
            POLYA=Yes.
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /clone="IMAGE:7341710"
                     /clone_lib="SAMN00170072 NCI_CGAP_DZ0"
                     /dev_stage="adult"
                     /lab_host="DH10B (Life Technologies)"
                     /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI;
                     UI-R-DZ0 is a non-normalized Rat cartilaginous tumor
                     library (RCT) constructed in pT3T7 PAC vector according to
                     the procedure described by Bonaldo, Lennon & Soares
                     (Genome Research Genome 6: 791-806, 1996). The
                     oligonucleotide used to prime first strand synthesis
                     contained the sequence tag CATTCTTGTA between the Not I
                     cloning site and dT18 stretch. The Rat cartilaginous tumor
                     tissue was provided by Dr Jeff Stevens at the University
                     of Iowa.
                     TAG_LIB=UI-R-DZ0;
                     TAG_SEQ=CATTCTTGTA;
                     TAG_TISSUE=rat SRC-JWST tumor line"
ORIGIN      
        1 tttttttttt ttttttttct ttctttcctt tccgagcctt ttgaggctga ggtctatttg
       61 tcagctgata tcccaactat ttaagactaa cagttaaaat aacagtcagt gtatgagaga
      121 gttaatgtgc ttggccacta ctaaggatga accagctagg gcttcttcaa gtcctcagct
      181 caccgcggtc cgttggccct gatcagttgg cttgtcctgc aatatggttt tcgctgtcac
      241 tcccatagtc gacgtcgggg tcctcctcct cctcctcgta ctcctcataa atctctccgt
      301 tgatgtcctc agcctggatt tcttccttga tgtgaggctc ctctcccttc acgccgtaag
      361 tgctctgaat cacgggcatc ccgggctctg ggctgctgga cacgctcgag tgctctgagg
      421 gcaggtgagg ggacggcatt cctgccatgg cgatggcacc ggggtacacg acaccggccg
      481 tagcgatggg gatctgtgct tgttgcccaa cgttgaagta ctgtcgcatt tcctgcctcc
      541 gattccgcat gatggccttg tactctccaa tgcgcagttt cttcncatct accagacaag
      601 tgcgcttcgg cctgngtttg tacttgtagt ctgggtactt ctccaggtgc tgtttgctga
      661 gcccgggctg ctcctcatag tatggctgtt tctctangtt ggtcatagct ttccaacgag
      721 atcccagtat cttgctgatn gttggatg
//
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