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UI-R-FJ0-cpy-e-19-0-UI.s1 UI-R-FJ0 Rattus norvegicus cDNA clone UI-R-FJ0-cpy-e-19-0-UI 3', mRNA sequence

GenBank: CA505052.1

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LOCUS       CA505052                 718 bp    mRNA    linear   EST 27-JAN-2011
DEFINITION  UI-R-FJ0-cpy-e-19-0-UI.s1 UI-R-FJ0 Rattus norvegicus cDNA clone
            UI-R-FJ0-cpy-e-19-0-UI 3', mRNA sequence.
ACCESSION   CA505052
VERSION     CA505052.1
DBLINK      BioSample: SAMN00171765
KEYWORDS    EST.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Bonaldo,M.F., Lennon,G. and Soares,M.B.
  TITLE     Normalization and subtraction: two approaches to facilitate gene
            discovery
  JOURNAL   Genome Res. 6 (9), 791-806 (1996)
   PUBMED   8889548
COMMENT     Contact: Soares, MB
            Cancer Biology & Epigenomics Program
            Children's Memorial Research Center
            2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA
            Tel: 773 755 6551
            Fax: 773 755 6378
            Email: mbsoares@childrensmemorial.org
            Tissue Procurement: Dr. James Lin, Universtiy of Iowa
             cDNA Library preparation: Dr. M. Bento Soares, University of Iowa
             cDNA Library Arrayed by: Dr. M. Bento Soares, University of Iowa
             DNA Sequencing by: Dr. M. Bento Soares, University of Iowa
             Clone Distribution: Researchers may obtain clones from Research
            Genetics (www.resgen.com).
            
            Seq primer: M13 FORWARD
            POLYA=Yes.
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /clone="UI-R-FJ0-cpy-e-19-0-UI"
                     /tissue_type="embryo"
                     /clone_lib="SAMN00171765 UI-R-FJ0"
                     /dev_stage="embryo"
                     /lab_host="DH10B (Life Technologies) (T1 phage resistant)"
                     /note="Vector: pYX-Asc; Site_1: EcoR I; Site_2: Not I;
                     UI-R-FJ0 is a cDNA library containing the following
                     tissue(s): rat embryo. The library was constructed
                     according to Bonaldo, Lennon and Soares, Genome Research,
                     6:791-806, 1996. First strand cDNA synthesis was primed
                     with an oligo-dT primer containing a Not I site. Double
                     stranded cDNA was ligated to an EcoR I adaptor, digested
                     with Not I, and cloned directionally into pT7T3-Pac
                     vector. The oligonucleotide used to prime the synthesis of
                     first-strand cDNA contains a library tag sequence that is
                     located between the Not I site and the (dT)18 tail. The
                     sequence tag for this library is CATCTCTACT. This library
                     was created for the University of Iowa Program for Rat
                     Gene Discovery and Mapping (Val Sheffield, Bento Soares
                     and Tom Casavant);
                     TAG_LIB=UI-R-FJ0;
                     TAG_SEQ=CATCTCTACT;
                     TAG_TISSUE=rat-embryo"
ORIGIN      
        1 tttttttttt ttttctttag gaccagattt atttgcttgc ttaaaacaaa ggaagacaaa
       61 gcaaagcttc actttttttt tattttccac aaaagattta tctttaagat gcaaattcat
      121 tacaatggaa gcctctgtag ttgctgtacc tgtcactaga agcacaggag ggaggtgtac
      181 tacaaagtag atcacagtct ccattgctgt acaatgaaag gagagtggag actccccatg
      241 gccaactgat agctcatatc ctttcctaga catatacttc ctaatagcac aagaacacac
      301 ttaccgttta caaattagaa aaggaattaa aaagcaaaat atttgagaat aatccctcct
      361 gaaatcattc attactgtgt agctgttttt ctacattaac gactacatgg acctgccttc
      421 atactggcca tgtttatttg tagagccaag gaaaacagtc tttgctaaga tcaagaaaat
      481 gtatacatga aaacaagcat cttaaaacag accttcttta tagcatttga aactgagtgt
      541 aaggatcgtc cggtttttat ccttaacaga gaacttcagt agctctaaag tgtaaaaagg
      601 ttggaaaatg gcttgtgtgt cacatcccag agagttccca aggctgggcc ctcagcccca
      661 gtcttcagag agtcaatgta aaagtaagca gagaaaaatg ggaggaaaag tgtttgta
//
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