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GenBank: CA505052.1
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LOCUS CA505052 718 bp mRNA linear EST 27-JAN-2011 DEFINITION UI-R-FJ0-cpy-e-19-0-UI.s1 UI-R-FJ0 Rattus norvegicus cDNA clone UI-R-FJ0-cpy-e-19-0-UI 3', mRNA sequence. ACCESSION CA505052 VERSION CA505052.1 DBLINK BioSample: SAMN00171765 KEYWORDS EST. SOURCE Rattus norvegicus (Norway rat) ORGANISM Rattus norvegicus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus. REFERENCE 1 (bases 1 to 718) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) PUBMED 8889548 COMMENT Contact: Soares, MB Cancer Biology & Epigenomics Program Children's Memorial Research Center 2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA Tel: 773 755 6551 Fax: 773 755 6378 Email: mbsoares@childrensmemorial.org Tissue Procurement: Dr. James Lin, Universtiy of Iowa cDNA Library preparation: Dr. M. Bento Soares, University of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, University of Iowa DNA Sequencing by: Dr. M. Bento Soares, University of Iowa Clone Distribution: Researchers may obtain clones from Research Genetics (www.resgen.com). Seq primer: M13 FORWARD POLYA=Yes. FEATURES Location/Qualifiers source 1..718 /organism="Rattus norvegicus" /mol_type="mRNA" /strain="Sprague-Dawley" /db_xref="taxon:10116" /clone="UI-R-FJ0-cpy-e-19-0-UI" /tissue_type="embryo" /clone_lib="SAMN00171765 UI-R-FJ0" /dev_stage="embryo" /lab_host="DH10B (Life Technologies) (T1 phage resistant)" /note="Vector: pYX-Asc; Site_1: EcoR I; Site_2: Not I; UI-R-FJ0 is a cDNA library containing the following tissue(s): rat embryo. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is CATCTCTACT. This library was created for the University of Iowa Program for Rat Gene Discovery and Mapping (Val Sheffield, Bento Soares and Tom Casavant); TAG_LIB=UI-R-FJ0; TAG_SEQ=CATCTCTACT; TAG_TISSUE=rat-embryo" ORIGIN 1 tttttttttt ttttctttag gaccagattt atttgcttgc ttaaaacaaa ggaagacaaa 61 gcaaagcttc actttttttt tattttccac aaaagattta tctttaagat gcaaattcat 121 tacaatggaa gcctctgtag ttgctgtacc tgtcactaga agcacaggag ggaggtgtac 181 tacaaagtag atcacagtct ccattgctgt acaatgaaag gagagtggag actccccatg 241 gccaactgat agctcatatc ctttcctaga catatacttc ctaatagcac aagaacacac 301 ttaccgttta caaattagaa aaggaattaa aaagcaaaat atttgagaat aatccctcct 361 gaaatcattc attactgtgt agctgttttt ctacattaac gactacatgg acctgccttc 421 atactggcca tgtttatttg tagagccaag gaaaacagtc tttgctaaga tcaagaaaat 481 gtatacatga aaacaagcat cttaaaacag accttcttta tagcatttga aactgagtgt 541 aaggatcgtc cggtttttat ccttaacaga gaacttcagt agctctaaag tgtaaaaagg 601 ttggaaaatg gcttgtgtgt cacatcccag agagttccca aggctgggcc ctcagcccca 661 gtcttcagag agtcaatgta aaagtaagca gagaaaaatg ggaggaaaag tgtttgta //
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