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UI-H-FH0-bcc-o-10-0-UI.s1 NCI_CGAP_FH0 Homo sapiens cDNA clone UI-H-FH0-bcc-o-10-0-UI 3', mRNA sequence

GenBank: CA418014.1

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LOCUS       CA418014                 685 bp    mRNA    linear   EST 07-JAN-2011
DEFINITION  UI-H-FH0-bcc-o-10-0-UI.s1 NCI_CGAP_FH0 Homo sapiens cDNA clone
            UI-H-FH0-bcc-o-10-0-UI 3', mRNA sequence.
ACCESSION   CA418014
VERSION     CA418014.1
DBLINK      BioSample: SAMN00170789
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 685)
  CONSRTM   NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap
  TITLE     National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
            Tumor Gene Index
  JOURNAL   Unpublished
COMMENT     Contact: Robert Strausberg, Ph.D.
            Email: cgapbs-r@mail.nih.gov
            Tissue Procurement: James Martin
             cDNA Library preparation: Dr. M. Bento Soares, University of Iowa
             cDNA Library Arrayed by: Dr. M. Bento Soares, University of Iowa
             DNA Sequencing by: Dr. M. Bento Soares, University of Iowa
             Clone Distribution: Clone distribution information can be obtained
            from Dr. M. Bento Soares, bento-soares@uiowa.edu
             The following repetitive elements were found in this cDNA
            sequence: 1-28, >AT_rich#Low_complexity 585-677, >LINE2
            Seq primer: M13 FORWARD
            POLYA=Yes.
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="UI-H-FH0-bcc-o-10-0-UI"
                     /tissue_type="Human Chondrosarcoma Cell Line"
                     /clone_lib="SAMN00170789 NCI_CGAP_FH0"
                     /dev_stage="Adult"
                     /lab_host="DH10B (Life Technologies)"
                     /note="Organ: Bone; Vector: pT7T3-Pac (Pharmacia) with a
                     modified polylinker; Site_1: EcoR I; Site_2: Not I;
                     NCI_CGAP_FH0 is a cDNA library containing the following
                     tissue(s): Human Grade 1 Chondrosarcoma Cell Line The
                     library was constructed according to Bonaldo, Lennon and
                     Soares, Genome Research, 6:791-806, 1996. First strand
                     cDNA synthesis was primed with an oligo-dT primer
                     containing a Not I site. Double stranded cDNA was ligated
                     to an EcoR I adaptor, digested with Not I, and cloned
                     directionally into pT7T3-Pac vector. The oligonucleotide
                     used to prime the synthesis of first-strand cDNA contains
                     a library tag sequence that is located between the Not I
                     site and the (dT)18 tail. The sequence tag for this
                     library is AGAATCCGGC. The cell line was provided by Dr
                     James Martin from University of Iowa;
                     TAG_LIB=UI-H-FH0;
                     TAG_SEQ=AGAATCCGGC;
                     TAG_TISSUE=Human Chondrosarcoma Cell Line CS8 - Grade 1
                     Chondrosarcoma"
ORIGIN      
        1 tttttttttt ttttttttaa cttttaaaca cattttatga tcccacatct atatctatac
       61 ataacatttg ccactgctta aacaaaacaa aatctgagct ttaaactcca aatggaatta
      121 cagaggagtc ttgatgtctg tgttccgcag ttctgtcatg tttgaaattg tcaaataaac
      181 atgctgcctt tccagtagcc actcagaatt cctcctctct gtccacaaag cattgtcacc
      241 ctccctgtcc catttgcccc agcccggtgg agcttgctga gctggcacca tcatctcttg
      301 taatagacaa agacattgag gaccagagag gggaagcacc ttgttgacat cccacagctc
      361 tgagtcaaag tggactttga ctcccttact agctgtttcc taaagtccag ggagccctgg
      421 aggcccagga atgtaagggc tggagcctgg ggctttctgt ccagaagtag gggaagtggg
      481 gctacagcta acggcttgag tgcatttcct ctcaagtaca ataagggctg ctttgttaga
      541 tgcacaatat aaatggctgc ctgcaaattt gtcaggtaat gtgtttgtct cccctatggg
      601 actataaact tggtataagc agggacctgt ctgaatcacc ctagtgtccc cagcaccagg
      661 ccagagcctg acatataaaa tgact
//
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