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GenBank: CA418014.1
FASTA Graphics
LOCUS CA418014 685 bp mRNA linear EST 07-JAN-2011 DEFINITION UI-H-FH0-bcc-o-10-0-UI.s1 NCI_CGAP_FH0 Homo sapiens cDNA clone UI-H-FH0-bcc-o-10-0-UI 3', mRNA sequence. ACCESSION CA418014 VERSION CA418014.1 DBLINK BioSample: SAMN00170789 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 685) CONSRTM NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: James Martin cDNA Library preparation: Dr. M. Bento Soares, University of Iowa cDNA Library Arrayed by: Dr. M. Bento Soares, University of Iowa DNA Sequencing by: Dr. M. Bento Soares, University of Iowa Clone Distribution: Clone distribution information can be obtained from Dr. M. Bento Soares, bento-soares@uiowa.edu The following repetitive elements were found in this cDNA sequence: 1-28, >AT_rich#Low_complexity 585-677, >LINE2 Seq primer: M13 FORWARD POLYA=Yes. FEATURES Location/Qualifiers source 1..685 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="UI-H-FH0-bcc-o-10-0-UI" /tissue_type="Human Chondrosarcoma Cell Line" /clone_lib="SAMN00170789 NCI_CGAP_FH0" /dev_stage="Adult" /lab_host="DH10B (Life Technologies)" /note="Organ: Bone; Vector: pT7T3-Pac (Pharmacia) with a modified polylinker; Site_1: EcoR I; Site_2: Not I; NCI_CGAP_FH0 is a cDNA library containing the following tissue(s): Human Grade 1 Chondrosarcoma Cell Line The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is AGAATCCGGC. The cell line was provided by Dr James Martin from University of Iowa; TAG_LIB=UI-H-FH0; TAG_SEQ=AGAATCCGGC; TAG_TISSUE=Human Chondrosarcoma Cell Line CS8 - Grade 1 Chondrosarcoma" ORIGIN 1 tttttttttt ttttttttaa cttttaaaca cattttatga tcccacatct atatctatac 61 ataacatttg ccactgctta aacaaaacaa aatctgagct ttaaactcca aatggaatta 121 cagaggagtc ttgatgtctg tgttccgcag ttctgtcatg tttgaaattg tcaaataaac 181 atgctgcctt tccagtagcc actcagaatt cctcctctct gtccacaaag cattgtcacc 241 ctccctgtcc catttgcccc agcccggtgg agcttgctga gctggcacca tcatctcttg 301 taatagacaa agacattgag gaccagagag gggaagcacc ttgttgacat cccacagctc 361 tgagtcaaag tggactttga ctcccttact agctgtttcc taaagtccag ggagccctgg 421 aggcccagga atgtaagggc tggagcctgg ggctttctgt ccagaagtag gggaagtggg 481 gctacagcta acggcttgag tgcatttcct ctcaagtaca ataagggctg ctttgttaga 541 tgcacaatat aaatggctgc ctgcaaattt gtcaggtaat gtgtttgtct cccctatggg 601 actataaact tggtataagc agggacctgt ctgaatcacc ctagtgtccc cagcaccagg 661 ccagagcctg acatataaaa tgact //
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