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UI-E-EJ1-ajs-m-06-0-UI.r1 UI-E-EJ1 Homo sapiens cDNA clone UI-E-EJ1-ajs-m-06-0-UI 5', mRNA sequence

GenBank: BQ185982.1

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LOCUS       BQ185982                 567 bp    mRNA    linear   EST 27-JAN-2011
DEFINITION  UI-E-EJ1-ajs-m-06-0-UI.r1 UI-E-EJ1 Homo sapiens cDNA clone
            UI-E-EJ1-ajs-m-06-0-UI 5', mRNA sequence.
ACCESSION   BQ185982
VERSION     BQ185982.1
DBLINK      BioSample: SAMN00170184
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Bonaldo,M.F., Lennon,G. and Soares,M.B.
  TITLE     Normalization and subtraction: two approaches to facilitate gene
            discovery
  JOURNAL   Genome Res. 6 (9), 791-806 (1996)
   PUBMED   8889548
COMMENT     Contact: Soares, MB
            Cancer Biology & Epigenomics Program
            Children's Memorial Research Center
            2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA
            Tel: 773 755 6551
            Fax: 773 755 6378
            Email: mbsoares@childrensmemorial.org
            Tissue Procurement: Dr. Gregg Hageman
             cDNA Library preparation: Dr. M. Bento Soares, University of Iowa
             cDNA Library Arrayed by: Dr. M. Bento Soares, University of Iowa
             DNA Sequencing by: Dr. M. Bento Soares, University of Iowa
             Clone Distribution: Researchers may obtain clones from Research
            Genetics (www.resgen.com).
             The following repetitive elements were found in this cDNA
            sequence: 527-563, >POLY_A#Simple_repeat
            Seq primer: M13 REVERSE.
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="UI-E-EJ1-ajs-m-06-0-UI"
                     /tissue_type="fetal eyes, lens, eye anterior segment,
                     optic nerve, retina, Retina Foveal and Macular, RPE and
                     Choroid"
                     /clone_lib="SAMN00170184 UI-E-EJ1"
                     /dev_stage="fetal and adult"
                     /lab_host="DH10B (Life Technologies) (T1 phage resistant)"
                     /note="Organ: eye; Vector: pT7T3-Pac (Pharmacia) with a
                     modified polylinker; Site_1: EcoR I; Site_2: Not I;
                     UI-E-EJ1 is a subtracted cDNA library constructed
                     according to Bonaldo, Lennon and Soares, Genome Research,
                     6:791-806, 1996. First strand cDNA synthesis was primed
                     with an oligo-dT primer containing a Not I site. Double
                     stranded cDNA was ligated to an EcoR I adaptor, digested
                     with Not I, and cloned directionally into pT7T3-Pac
                     vector. The oligonucleotide used to prime the synthesis of
                     first-strand cDNA contains a library tag sequence that is
                     located between the Not I site and the (dT)18 tail. The
                     sequence tags for this library are: fetal eyes,
                     AGAATCAAGA; lens, CGATTAGCGA; eye anterior segment,
                     AATGCCGCAT; optic nerve, CCATTAAGTG; retina, CCGCG; Retina
                     Foveal and Macular, GTCC; RPE and Choroid, ACCTA. This
                     library was created for the program, Gene Discovery in the
                     Visual System, supported by National Eye Institute (NEI)."
ORIGIN      
        1 gcgggctgag gcactnttcg ggcaacaccc aatactcggg gctccgctcg gcttcttcgc
       61 gccgagatgc ctaagaaggc tggtgcgacg accaagggta aaagccagag caaggaacca
      121 gagagaccac ttcctccctt aggtcctgtg gcagttgatc ctaaaggatg cgtcaccata
      181 gccatccatg caaaacctgg ctccaaacaa aatgctgtaa cagatttgac agcagaggct
      241 gtaaatgtag ctattgcagc acctccatca gagggagagg ctaatgctga gctctgtcgg
      301 tatctttcca aggtcctaga actcaggaag agtgatgtgg ttttggataa gctctgctat
      361 tccattcctc aacaattgga ggagactcag gagggcgttt caggtattcc tgatactcct
      421 tgtcattttc tgtgaatcta ctagcaaaca tctcttcaaa ctttggaaca gcttcggcag
      481 tgtcagtcat tctgaaaatc tgtacaattt taaagacatt tccattaaaa aaaatttaaa
      541 aattaaaaaa aaaaaaaaaa aaatagg
//
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