LOCUS BQ078334 653 bp mRNA linear EST 02-APR-2002
DEFINITION fy79d03.x1 Sugano SJD adult male Danio rerio cDNA clone
IMAGE:5777309 3', mRNA sequence.
ACCESSION BQ078334
VERSION BQ078334.1
DBLINK BioSample: SAMN00164468
KEYWORDS EST.
SOURCE Danio rerio (zebrafish)
ORGANISM Danio rerio
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Actinopterygii; Neopterygii; Teleostei; Ostariophysi;
Cypriniformes; Danionidae; Danioninae; Danio.
REFERENCE 1 (bases 1 to 653)
AUTHORS Clark,M., Johnson,S.L., Lehrach,H., Lee,R., Li,F., Marra,M.,
Eddy,S., Hillier,L., Kucaba,T., Martin,J., Beck,C., Wylie,T.,
Underwood,K., Steptoe,M., Theising,B., Allen,M., Bowers,Y.,
Person,B., Swaller,T., Gibbons,M., Pape,D., Harvey,N., Schurk,R.,
Ritter,E., Kohn,S., Shin,T., Jackson,Y., Cardenas,M., McCann,R.,
Waterston,R. and Wilson,R.
TITLE WashU Zebrafish EST Project 1998
JOURNAL Unpublished
COMMENT Contact: Stephen L. Johnson
Washington University School of Medicine
4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
Tel: 314 286 1800
Fax: 314 286 1810
Email: zbrafish@watson.wustl.edu
Library constructed by Dr. Sumio Sugano and Dr. Koichi Kawakami DNA
Sequencing by: Washington University Genome Sequencing Center Clone
distribution information can be found through the I.M.A.G.E.
Consortium/LLNL, send email to: info@image.llnl.gov
Seq primer: T7 from Gibco
High quality sequence stop: 537.
FEATURES Location/Qualifiers
source 1..653
/organism="Danio rerio"
/mol_type="mRNA"
/db_xref="taxon:7955"
/clone="IMAGE:5777309"
/sex="male"
/tissue_type="whole body"
/clone_lib="SAMN00164468 Sugano SJD adult male"
/dev_stage="adult"
/lab_host="DH10B (phage resistant)"
/note="Vector: pME18S-FL3; Site_1: DraIII (CACCATGTG);
Site_2: DraIII (CACTGTGTG); 1st strand cDNA was primed
with an oligo(dT) primer [ATGTGGCCTTTTTTTTTTTTTTTTT];
double-stranded cDNA was ligated to a DraIII adaptor
[TGTTGGCCTACTGG], digested and cloned into distinct DraIII
sites of the pME18S-FL3 vector (5' site CACTGTGTG, 3' site
CACCATGTG). XhoI should be used to isolate the cDNA
insert. Size selection was performed to exclude fragments
<1.5kb. Library constructed and donated by Dr. Sumio
Sugano (University of Tokyo Institute of Medical Science).
Custom primers for sequencing: 5' end primer
CTTCTGCTCTAAAAGCTGCG and 3' end primer
CGACCTGCAGCTCGAGCACA."
ORIGIN
1 tttttttttt tttcaatgaa ttcgctttat tacccacagc ttttttacag tgtacaggtg
61 tagtgcatta agcttttatg tgtaaactac ttagcaactt cactggtggg gaacaagtag
121 tgtattcacc gttacaactt atacttacat aaattattaa atatcttatt tggcaagatt
181 atacaaatgg gcaggaagaa aatagacaag tcagtgtgca tcagtttgcc cactacgcta
241 caagactgga tggaggaata ctgcacaagt aacaaaatat tcatctgttt agtacggcgt
301 gtactcggca ttatacatgt agctttactt aacacaatat agcaatagat catctgtgaa
361 agataggaat gtacagaaag gtaaggtgga tgaaagcaca agggtagaac atctacagca
421 ttaatgctta caattactgc aaaagcacag atgcatttac aatcccacca taaatgaggc
481 agcgctgatg gtcacggcgt gcttgtgcca actctttttc aaatttactc aagttctaag
541 gaacattcca cttatttcga gaaaattggc tcattttaca agtcacctag agttaaatat
601 tagcattttt aaatccgctc agaaaatctc ttttagcact tttagcttag ctt
//