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fy79d03.x1 Sugano SJD adult male Danio rerio cDNA clone IMAGE:5777309 3', mRNA sequence

GenBank: BQ078334.1

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LOCUS       BQ078334                 653 bp    mRNA    linear   EST 02-APR-2002
DEFINITION  fy79d03.x1 Sugano SJD adult male Danio rerio cDNA clone
            IMAGE:5777309 3', mRNA sequence.
ACCESSION   BQ078334
VERSION     BQ078334.1
DBLINK      BioSample: SAMN00164468
KEYWORDS    EST.
SOURCE      Danio rerio (zebrafish)
  ORGANISM  Danio rerio
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Ostariophysi;
            Cypriniformes; Danionidae; Danioninae; Danio.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Clark,M., Johnson,S.L., Lehrach,H., Lee,R., Li,F., Marra,M.,
            Eddy,S., Hillier,L., Kucaba,T., Martin,J., Beck,C., Wylie,T.,
            Underwood,K., Steptoe,M., Theising,B., Allen,M., Bowers,Y.,
            Person,B., Swaller,T., Gibbons,M., Pape,D., Harvey,N., Schurk,R.,
            Ritter,E., Kohn,S., Shin,T., Jackson,Y., Cardenas,M., McCann,R.,
            Waterston,R. and Wilson,R.
  TITLE     WashU Zebrafish EST Project 1998
  JOURNAL   Unpublished
COMMENT     Contact: Stephen L. Johnson
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: zbrafish@watson.wustl.edu
            Library constructed by Dr. Sumio Sugano and Dr. Koichi Kawakami DNA
            Sequencing by: Washington University Genome Sequencing Center Clone
            distribution information can be found through the I.M.A.G.E.
            Consortium/LLNL, send email to: info@image.llnl.gov
            Seq primer: T7 from Gibco
            High quality sequence stop: 537.
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Danio rerio"
                     /mol_type="mRNA"
                     /db_xref="taxon:7955"
                     /clone="IMAGE:5777309"
                     /sex="male"
                     /tissue_type="whole body"
                     /clone_lib="SAMN00164468 Sugano SJD adult male"
                     /dev_stage="adult"
                     /lab_host="DH10B (phage resistant)"
                     /note="Vector: pME18S-FL3; Site_1: DraIII (CACCATGTG);
                     Site_2: DraIII (CACTGTGTG); 1st strand cDNA was primed
                     with an oligo(dT) primer [ATGTGGCCTTTTTTTTTTTTTTTTT];
                     double-stranded cDNA was ligated to a DraIII adaptor
                     [TGTTGGCCTACTGG], digested and cloned into distinct DraIII
                     sites of the pME18S-FL3 vector (5' site CACTGTGTG, 3' site
                     CACCATGTG). XhoI should be used to isolate the cDNA
                     insert. Size selection was performed to exclude fragments
                     <1.5kb. Library constructed and donated by Dr. Sumio
                     Sugano (University of Tokyo Institute of Medical Science).
                     Custom primers for sequencing: 5' end primer
                     CTTCTGCTCTAAAAGCTGCG and 3' end primer
                     CGACCTGCAGCTCGAGCACA."
ORIGIN      
        1 tttttttttt tttcaatgaa ttcgctttat tacccacagc ttttttacag tgtacaggtg
       61 tagtgcatta agcttttatg tgtaaactac ttagcaactt cactggtggg gaacaagtag
      121 tgtattcacc gttacaactt atacttacat aaattattaa atatcttatt tggcaagatt
      181 atacaaatgg gcaggaagaa aatagacaag tcagtgtgca tcagtttgcc cactacgcta
      241 caagactgga tggaggaata ctgcacaagt aacaaaatat tcatctgttt agtacggcgt
      301 gtactcggca ttatacatgt agctttactt aacacaatat agcaatagat catctgtgaa
      361 agataggaat gtacagaaag gtaaggtgga tgaaagcaca agggtagaac atctacagca
      421 ttaatgctta caattactgc aaaagcacag atgcatttac aatcccacca taaatgaggc
      481 agcgctgatg gtcacggcgt gcttgtgcca actctttttc aaatttactc aagttctaag
      541 gaacattcca cttatttcga gaaaattggc tcattttaca agtcacctag agttaaatat
      601 tagcattttt aaatccgctc agaaaatctc ttttagcact tttagcttag ctt
//
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