LOCUS BM095818 594 bp mRNA linear EST 20-NOV-2001
DEFINITION fv27a12.x1 Sugano SJD adult male Danio rerio cDNA clone
IMAGE:5409694 3', mRNA sequence.
ACCESSION BM095818
VERSION BM095818.1
DBLINK BioSample: SAMN00164468
KEYWORDS EST.
SOURCE Danio rerio (zebrafish)
ORGANISM Danio rerio
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Actinopterygii; Neopterygii; Teleostei; Ostariophysi;
Cypriniformes; Danionidae; Danioninae; Danio.
REFERENCE 1 (bases 1 to 594)
AUTHORS Clark,M., Johnson,S.L., Lehrach,H., Lee,R., Li,F., Marra,M.,
Eddy,S., Hillier,L., Kucaba,T., Martin,J., Beck,C., Wylie,T.,
Underwood,K., Steptoe,M., Theising,B., Allen,M., Bowers,Y.,
Person,B., Swaller,T., Gibbons,M., Pape,D., Harvey,N., Schurk,R.,
Ritter,E., Kohn,S., Shin,T., Jackson,Y., Cardenas,M., McCann,R.,
Waterston,R. and Wilson,R.
TITLE WashU Zebrafish EST Project 1998
JOURNAL Unpublished
COMMENT Contact: Stephen L. Johnson
Washington University School of Medicine
4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
Tel: 314 286 1800
Fax: 314 286 1810
Email: zbrafish@watson.wustl.edu
Library constructed by Dr. Sumio Sugano and Dr. Koichi Kawakami.
DNA Sequencing by: Washington University Genome Sequencing Center
Clone distribution information can be found through the I.M.A.G.E.
Consortium/LLNL, send email to: info@image.llnl.gov
High quality sequence stop: 467.
FEATURES Location/Qualifiers
source 1..594
/organism="Danio rerio"
/mol_type="mRNA"
/db_xref="taxon:7955"
/clone="IMAGE:5409694"
/sex="male"
/tissue_type="whole body"
/clone_lib="SAMN00164468 Sugano SJD adult male"
/dev_stage="adult"
/lab_host="DH10B (phage resistant)"
/note="Vector: pME18S-FL3; Site_1: DraIII (CACCATGTG);
Site_2: DraIII (CACTGTGTG); 1st strand cDNA was primed
with an oligo(dT) primer [ATGTGGCCTTTTTTTTTTTTTTTTT];
double-stranded cDNA was ligated to a DraIII adaptor
[TGTTGGCCTACTGG], digested and cloned into distinct DraIII
sites of the pME18S-FL3 vector (5' site CACTGTGTG, 3' site
CACCATGTG). XhoI should be used to isolate the cDNA
insert. Size selection was performed to exclude fragments
<1.5kb. Library constructed and donated by Dr. Sumio
Sugano (University of Tokyo Institute of Medical Science).
Custom primers for sequencing: 5' end primer
CTTCTGCTCTAAAAGCTGCG and 3' end primer
CGACCTGCAGCTCGAGCACA."
ORIGIN
1 cattgaaaaa acaacaccaa caaacacgta gtaaacagag ttaaattaaa gggtttaaac
61 acagtcaagg aatgcaatga tcaccctaag attagggtca gtgcaaatga aacaaaaagc
121 attggcgatt cggaatcctc ataaatgaga accataagac aggcatgtca atcatgtatg
181 aatacgacta cagtatctga agagggcgaa acaaattgaa ctcgaacaca gaataagctt
241 tcaaacaagt ccgcttagag aaatattgaa aaagattact tgcctgttta caatacaata
301 caatactgct atcccatttg tatctccttt tacactagaa gaaaaggata ttttgcacta
361 gcacctcaca aggctttgtg ggtttatggg ttttatggta acacatgaat catatattaa
421 gttttacaca atagaagtcc taatcaatac tgacctccag cagtatcttg ctaaatatat
481 acagacagag ctatcaaaat gcttatattg cataacaatg caaaagaaag cttgtaaatc
541 cagaccgcac gctgttgagg aaaacagcca cagtctgaat aagtcaataa ggaa
//