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fv27a12.x1 Sugano SJD adult male Danio rerio cDNA clone IMAGE:5409694 3', mRNA sequence

GenBank: BM095818.1

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LOCUS       BM095818                 594 bp    mRNA    linear   EST 20-NOV-2001
DEFINITION  fv27a12.x1 Sugano SJD adult male Danio rerio cDNA clone
            IMAGE:5409694 3', mRNA sequence.
ACCESSION   BM095818
VERSION     BM095818.1
DBLINK      BioSample: SAMN00164468
KEYWORDS    EST.
SOURCE      Danio rerio (zebrafish)
  ORGANISM  Danio rerio
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Ostariophysi;
            Cypriniformes; Danionidae; Danioninae; Danio.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Clark,M., Johnson,S.L., Lehrach,H., Lee,R., Li,F., Marra,M.,
            Eddy,S., Hillier,L., Kucaba,T., Martin,J., Beck,C., Wylie,T.,
            Underwood,K., Steptoe,M., Theising,B., Allen,M., Bowers,Y.,
            Person,B., Swaller,T., Gibbons,M., Pape,D., Harvey,N., Schurk,R.,
            Ritter,E., Kohn,S., Shin,T., Jackson,Y., Cardenas,M., McCann,R.,
            Waterston,R. and Wilson,R.
  TITLE     WashU Zebrafish EST Project 1998
  JOURNAL   Unpublished
COMMENT     Contact: Stephen L. Johnson
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: zbrafish@watson.wustl.edu
            Library constructed by Dr. Sumio Sugano and Dr. Koichi Kawakami.
            DNA Sequencing by: Washington University Genome Sequencing Center
            Clone distribution information can be found through the I.M.A.G.E.
            Consortium/LLNL, send email to: info@image.llnl.gov
            High quality sequence stop: 467.
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Danio rerio"
                     /mol_type="mRNA"
                     /db_xref="taxon:7955"
                     /clone="IMAGE:5409694"
                     /sex="male"
                     /tissue_type="whole body"
                     /clone_lib="SAMN00164468 Sugano SJD adult male"
                     /dev_stage="adult"
                     /lab_host="DH10B (phage resistant)"
                     /note="Vector: pME18S-FL3; Site_1: DraIII (CACCATGTG);
                     Site_2: DraIII (CACTGTGTG); 1st strand cDNA was primed
                     with an oligo(dT) primer [ATGTGGCCTTTTTTTTTTTTTTTTT];
                     double-stranded cDNA was ligated to a DraIII adaptor
                     [TGTTGGCCTACTGG], digested and cloned into distinct DraIII
                     sites of the pME18S-FL3 vector (5' site CACTGTGTG, 3' site
                     CACCATGTG). XhoI should be used to isolate the cDNA
                     insert. Size selection was performed to exclude fragments
                     <1.5kb. Library constructed and donated by Dr. Sumio
                     Sugano (University of Tokyo Institute of Medical Science).
                     Custom primers for sequencing: 5' end primer
                     CTTCTGCTCTAAAAGCTGCG and 3' end primer
                     CGACCTGCAGCTCGAGCACA."
ORIGIN      
        1 cattgaaaaa acaacaccaa caaacacgta gtaaacagag ttaaattaaa gggtttaaac
       61 acagtcaagg aatgcaatga tcaccctaag attagggtca gtgcaaatga aacaaaaagc
      121 attggcgatt cggaatcctc ataaatgaga accataagac aggcatgtca atcatgtatg
      181 aatacgacta cagtatctga agagggcgaa acaaattgaa ctcgaacaca gaataagctt
      241 tcaaacaagt ccgcttagag aaatattgaa aaagattact tgcctgttta caatacaata
      301 caatactgct atcccatttg tatctccttt tacactagaa gaaaaggata ttttgcacta
      361 gcacctcaca aggctttgtg ggtttatggg ttttatggta acacatgaat catatattaa
      421 gttttacaca atagaagtcc taatcaatac tgacctccag cagtatcttg ctaaatatat
      481 acagacagag ctatcaaaat gcttatattg cataacaatg caaaagaaag cttgtaaatc
      541 cagaccgcac gctgttgagg aaaacagcca cagtctgaat aagtcaataa ggaa
//
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