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GenBank: BI275514.1
FASTA Graphics
LOCUS BI275514 443 bp mRNA linear EST 27-JAN-2011 DEFINITION UI-R-CX0-bwy-b-08-0-UI.s1 UI-R-CX0 Rattus norvegicus cDNA clone UI-R-CX0-bwy-b-08-0-UI 3', mRNA sequence. ACCESSION BI275514 VERSION BI275514.1 DBLINK BioSample: SAMN00163267 KEYWORDS EST. SOURCE Rattus norvegicus (Norway rat) ORGANISM Rattus norvegicus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus. REFERENCE 1 (bases 1 to 443) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) PUBMED 8889548 COMMENT Contact: Soares, MB Cancer Biology & Epigenomics Program Children's Memorial Research Center 2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA Tel: 773 755 6551 Fax: 773 755 6378 Email: mbsoares@childrensmemorial.org The sequence contained an oligo-dT track that was present in the oligonucleotide that was used to prime the synthesis of first strand cDNA and therefore this may represent a bonafide poly A tail. The sequence tag present in the cDNA between the NotI site and the oligo-dT track served to identify it as a clone from the normalized rat placenta pool library cDNA Library Preparation: M.B. Soares Lab Clone distribution: clones will be available through Research Genetics (www.resgen.com) Seq primer: M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..443 /organism="Rattus norvegicus" /mol_type="mRNA" /strain="Sprague-Dawley" /db_xref="taxon:10116" /clone="UI-R-CX0-bwy-b-08-0-UI" /clone_lib="SAMN00163267 UI-R-CX0" /dev_stage="ADULT" /lab_host="DH10B (Life Technologies)" /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI; The UI-R-CX0 library is a normalized library constructed from the following rat placenta tissues: embryonic day 17, embryonic day 19, embryonic day 21. For a detailed description of the library from which this clone was derived, please visit our web site at ratest.eng.uiowa.edu. The subtraction has been previously described in (Bonaldo, Lennon and Soares, Genome Research 6:791-806, 1996); TAG_LIB=UI-R-CX0; TAG_SEQ=TCACGACAGT; TAG_TISSUE=rat placenta pool" ORIGIN 1 tttttttttt ttttttttca ccgtgtgcta tcaaagtttt atttatgcct cgttacacca 61 aaagtaaata gtcaattcaa aataaactaa tgacctgcat attttcatat gggtgaatga 121 atgtatatct aaataggaaa taaatggcaa tcctatctac ctatattaaa aaaaatagac 181 agtctttcca gatttttgca tactaaggag aggtctgcgg tcttaaggtc tcacaacggg 241 tgaccaggaa agcagcagtt agcccgcagc atcttaccat ctgactcaac tgttcctaaa 301 tgacaccatt taaaagatct gttaacacat tatctgtagt tattctgaga tggtcccgat 361 gttaacccta ggatctacca aaaagaatta gcagtacaat agagaagaac agataatcca 421 cctgactata catgtgctct ctt //
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