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UI-R-CX0-bwy-b-08-0-UI.s1 UI-R-CX0 Rattus norvegicus cDNA clone UI-R-CX0-bwy-b-08-0-UI 3', mRNA sequence

GenBank: BI275514.1

FASTA Graphics 

LOCUS       BI275514                 443 bp    mRNA    linear   EST 27-JAN-2011
DEFINITION  UI-R-CX0-bwy-b-08-0-UI.s1 UI-R-CX0 Rattus norvegicus cDNA clone
            UI-R-CX0-bwy-b-08-0-UI 3', mRNA sequence.
ACCESSION   BI275514
VERSION     BI275514.1
DBLINK      BioSample: SAMN00163267
KEYWORDS    EST.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Bonaldo,M.F., Lennon,G. and Soares,M.B.
  TITLE     Normalization and subtraction: two approaches to facilitate gene
            discovery
  JOURNAL   Genome Res. 6 (9), 791-806 (1996)
   PUBMED   8889548
COMMENT     Contact: Soares, MB
            Cancer Biology & Epigenomics Program
            Children's Memorial Research Center
            2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA
            Tel: 773 755 6551
            Fax: 773 755 6378
            Email: mbsoares@childrensmemorial.org
            The sequence contained an oligo-dT track that was present in the
            oligonucleotide that was used to prime the synthesis of first
            strand cDNA and therefore this may represent a bonafide poly A
            tail. The sequence tag present in the cDNA between the NotI site
            and the oligo-dT track served to identify it as a clone from the
            normalized rat placenta pool library cDNA Library Preparation: M.B.
            Soares Lab Clone distribution: clones will be available through
            Research Genetics (www.resgen.com)
            Seq primer: M13 Forward
            POLYA=Yes.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /clone="UI-R-CX0-bwy-b-08-0-UI"
                     /clone_lib="SAMN00163267 UI-R-CX0"
                     /dev_stage="ADULT"
                     /lab_host="DH10B (Life Technologies)"
                     /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI;
                     The UI-R-CX0 library is a normalized library constructed
                     from the following rat placenta tissues: embryonic day 17,
                     embryonic day 19, embryonic day 21. For a detailed
                     description of the library from which this clone was
                     derived, please visit our web site at
                     ratest.eng.uiowa.edu. The subtraction has been previously
                     described in (Bonaldo, Lennon and Soares, Genome Research
                     6:791-806, 1996);
                     TAG_LIB=UI-R-CX0;
                     TAG_SEQ=TCACGACAGT;
                     TAG_TISSUE=rat placenta pool"
ORIGIN      
        1 tttttttttt ttttttttca ccgtgtgcta tcaaagtttt atttatgcct cgttacacca
       61 aaagtaaata gtcaattcaa aataaactaa tgacctgcat attttcatat gggtgaatga
      121 atgtatatct aaataggaaa taaatggcaa tcctatctac ctatattaaa aaaaatagac
      181 agtctttcca gatttttgca tactaaggag aggtctgcgg tcttaaggtc tcacaacggg
      241 tgaccaggaa agcagcagtt agcccgcagc atcttaccat ctgactcaac tgttcctaaa
      301 tgacaccatt taaaagatct gttaacacat tatctgtagt tattctgaga tggtcccgat
      361 gttaacccta ggatctacca aaaagaatta gcagtacaat agagaagaac agataatcca
      421 cctgactata catgtgctct ctt
//
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