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602123551F1 NIH_MGC_56 Homo sapiens cDNA clone IMAGE:4280461 5', mRNA sequence

GenBank: BF668430.1

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LOCUS       BF668430                 821 bp    mRNA    linear   EST 21-DEC-2000
DEFINITION  602123551F1 NIH_MGC_56 Homo sapiens cDNA clone IMAGE:4280461 5',
            mRNA sequence.
ACCESSION   BF668430
VERSION     BF668430.1
DBLINK      BioSample: SAMN00161291
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 821)
  CONSRTM   National Institutes of Health, Mammalian Gene Collection (MGC)
  TITLE     NIH-MGC EST Sequencing Project
  JOURNAL   Unpublished
  REMARK    http://mgc.nci.nih.gov/
COMMENT     Contact: Robert Strausberg, Ph.D.
            Email: cgapbs-r@mail.nih.gov
            Tissue Procurement: ATCC
             cDNA Library Preparation: CLONETECH Laboratories, Inc.
             cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
             DNA Sequencing by: Incyte Genomics, Inc.
             Clone distribution: MGC clone distribution information can be
            found through the I.M.A.G.E. Consortium/LLNL at:
            http://image.llnl.gov
            
            Plate: LLCM1107  row: f  column: 14
            High quality sequence stop: 507.
FEATURES             Location/Qualifiers
     source          1..821
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:4280461"
                     /tissue_type="primitive neuroectoderm"
                     /clone_lib="SAMN00161291 NIH_MGC_56"
                     /lab_host="DH10B (T1 phage-resistant)"
                     /note="Organ: brain; Vector: pDNR-LIB (Clontech); Site_1:
                     SfiI (ggccgcctcggcc); Site_2: SfiI (ggccattatggcc);
                     Double-stranded cDNA was prepared from cell line RNA. 5'
                     and 3' adaptors were used in cloning as follows: 5'
                     adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor
                     sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3'
                     (where B = A, C, or G and N = A, C, G, or T). Average
                     insert size 1.65 kb (range 0.9-4.0 kb). 15/15 colonies
                     contained inserts by PCR. This library was enriched for
                     full-length clones and was constructed by Clontech
                     Laboratories (Palo Alto, CA)."
ORIGIN      
        1 gtcagggacc tagaggcgcc ggcgggtgga atccctgttt ccgacacccg gttgatgcgg
       61 tcgggagagc gagactggcc gagggaccgg cttaggcaaa ggcctggagg cacgactgcg
      121 ccaggaggat tcggaacaca gggacatctt gtgtccactg agaacaaagt atgagtcgga
      181 gttatttcct cagtaccagg ctgaggatga tctttgtgag gaccgtagaa gccatggaca
      241 gttttgaaca aaggaggaaa tctgtgttag agttaaagat tccgaaaaga tgttcaaagg
      301 aagaacggag tgtaaggcgg tgatgaggac agtgaggcat tgagaggttg aatatttgct
      361 caaggtcacg tagctggtaa gaggtgacat tgagcactgg gccacaagat tagagactca
      421 gcctgaggtc acctggctac agggccaaaa aaaagcttac agagaaacct gagtctatca
      481 aatcccagca gggacacatt tctgaaagct ccgctttgga acaccttctc atggtatggg
      541 acgtatttag gaacccacag gaagagtata gatggcaagt cctcaaggtc tcatcctcac
      601 tatatgtcta gaacttaggc cgggaatatt catagcgacg aaggtgcctg gtcccagtgg
      661 ggggtggacc gacctgggtc aattgggccc ggcagggagt ccatcagtcc gaagaagagg
      721 tatacacggg gggtcacaga acgccaacac cctgtgatgg cgggttggat tggatgaagc
      781 tgactatata caccaaacaa cacaacaccg agaccacacc a
//
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