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GenBank: BF668430.1
FASTA Graphics
LOCUS BF668430 821 bp mRNA linear EST 21-DEC-2000 DEFINITION 602123551F1 NIH_MGC_56 Homo sapiens cDNA clone IMAGE:4280461 5', mRNA sequence. ACCESSION BF668430 VERSION BF668430.1 DBLINK BioSample: SAMN00161291 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 821) CONSRTM National Institutes of Health, Mammalian Gene Collection (MGC) TITLE NIH-MGC EST Sequencing Project JOURNAL Unpublished REMARK http://mgc.nci.nih.gov/ COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: ATCC cDNA Library Preparation: CLONETECH Laboratories, Inc. cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) DNA Sequencing by: Incyte Genomics, Inc. Clone distribution: MGC clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov Plate: LLCM1107 row: f column: 14 High quality sequence stop: 507. FEATURES Location/Qualifiers source 1..821 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="IMAGE:4280461" /tissue_type="primitive neuroectoderm" /clone_lib="SAMN00161291 NIH_MGC_56" /lab_host="DH10B (T1 phage-resistant)" /note="Organ: brain; Vector: pDNR-LIB (Clontech); Site_1: SfiI (ggccgcctcggcc); Site_2: SfiI (ggccattatggcc); Double-stranded cDNA was prepared from cell line RNA. 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.65 kb (range 0.9-4.0 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA)." ORIGIN 1 gtcagggacc tagaggcgcc ggcgggtgga atccctgttt ccgacacccg gttgatgcgg 61 tcgggagagc gagactggcc gagggaccgg cttaggcaaa ggcctggagg cacgactgcg 121 ccaggaggat tcggaacaca gggacatctt gtgtccactg agaacaaagt atgagtcgga 181 gttatttcct cagtaccagg ctgaggatga tctttgtgag gaccgtagaa gccatggaca 241 gttttgaaca aaggaggaaa tctgtgttag agttaaagat tccgaaaaga tgttcaaagg 301 aagaacggag tgtaaggcgg tgatgaggac agtgaggcat tgagaggttg aatatttgct 361 caaggtcacg tagctggtaa gaggtgacat tgagcactgg gccacaagat tagagactca 421 gcctgaggtc acctggctac agggccaaaa aaaagcttac agagaaacct gagtctatca 481 aatcccagca gggacacatt tctgaaagct ccgctttgga acaccttctc atggtatggg 541 acgtatttag gaacccacag gaagagtata gatggcaagt cctcaaggtc tcatcctcac 601 tatatgtcta gaacttaggc cgggaatatt catagcgacg aaggtgcctg gtcccagtgg 661 ggggtggacc gacctgggtc aattgggccc ggcagggagt ccatcagtcc gaagaagagg 721 tatacacggg gggtcacaga acgccaacac cctgtgatgg cgggttggat tggatgaagc 781 tgactatata caccaaacaa cacaacaccg agaccacacc a //
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