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GenBank: BF556958.1
FASTA Graphics
LOCUS BF556958 575 bp mRNA linear EST 27-JAN-2011 DEFINITION UI-R-A1-ev-b-12-0-UI.r1 UI-R-A1 Rattus norvegicus cDNA clone UI-R-A1-ev-b-12-0-UI 5', mRNA sequence. ACCESSION BF556958 VERSION BF556958.1 DBLINK BioSample: SAMN00155772 KEYWORDS EST. SOURCE Rattus norvegicus (Norway rat) ORGANISM Rattus norvegicus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus. REFERENCE 1 (bases 1 to 575) AUTHORS Bonaldo,M.F., Lennon,G. and Soares,M.B. TITLE Normalization and subtraction: two approaches to facilitate gene discovery JOURNAL Genome Res. 6 (9), 791-806 (1996) PUBMED 8889548 COMMENT Contact: Soares, MB Cancer Biology & Epigenomics Program Children's Memorial Research Center 2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA Tel: 773 755 6551 Fax: 773 755 6378 Email: mbsoares@childrensmemorial.org cDNA Library Preparation: M.B. Soares Lab Clone distribution: clones will be available through Research Genetics (www.resgen.com) This clone is also available through the I.M.A.G.E. Consortium at LLNL (info@image.llnl.gov). IMAGE ID= 1771795 Seq primer: M13 Forward. FEATURES Location/Qualifiers source 1..575 /organism="Rattus norvegicus" /mol_type="mRNA" /strain="Sprague-Dawley" /db_xref="taxon:10116" /clone="UI-R-A1-ev-b-12-0-UI" /clone_lib="SAMN00155772 UI-R-A1" /dev_stage="adult" /lab_host="DH10B (Life Technologies)" /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI; The UI-R-A1 library is a subtracted library derived from the UI-R-A0 library. The UI-R-A0 library consisted of a mixture of individually tagged normalized libraries constructed from rat placenta, adult lung, brain, liver, kidney, heart, spleen, ovary, and muscle. The tag is a string of 3-5 nucleotides present between the Not I site and the oligo-dT track which allows identification of the library of origin of a clone within the mixture. The subtracted library (UI-R-A1) was constructed as follows: PCR amplified cDNA inserts from a pool of approximately 3,840 UI-R-A0 clones from which 3' ESTs had been derived was used as a driver in a hybridization with the UI-R-A0 library in the form of single-stranded circles. The remaining single-stranded circles (subtracted library) was purified by hydroxyapatite column chromatography, converted to double-stranded circles and electroporated into DH10B bacteria (Life Technologies) to generate the UI-R-A1 library. This procedure has been previously described (Bonaldo, Lennon and Soares, Genome Research 6: 791-806, 1996)" ORIGIN 1 cacgaggtgt ggttacattg gtgccttact ggctatcagg agacagtatc acagcattgt 61 tccagcactt tatgagtaca caagtcagct attaaaacgt cgagtggtgt cagtaccctt 121 gaaaattgag catctctctg aggaactgga tgcatggaga gcttgcacac agtccaccaa 181 acaattgtca gaagattctc catatgtacc cccttctgat tcacaaagaa cggtttatga 241 aactttatta aagagactaa aagaagagcc gctcaaagga attactggac cagattatgt 301 gactggaaca aatcttccaa gccattcaga cattcatatt tcttgtctta atggattaaa 361 aatccagggt cctgtgtatt ttctggaaga tgggaaatca gctatttctt tgaatgatgc 421 tctgatgtgg gcaaaggtga atccattctc acctttaaga actggaatac gcctcaatcc 481 attttgactg aagatttttc tccacactta gtagtttttg taaaaaggaa atttggggta 541 tttattacct tcattttggt tgtccaaaaa caata //
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