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UI-R-A1-ev-b-12-0-UI.r1 UI-R-A1 Rattus norvegicus cDNA clone UI-R-A1-ev-b-12-0-UI 5', mRNA sequence

GenBank: BF556958.1

FASTA Graphics 

LOCUS       BF556958                 575 bp    mRNA    linear   EST 27-JAN-2011
DEFINITION  UI-R-A1-ev-b-12-0-UI.r1 UI-R-A1 Rattus norvegicus cDNA clone
            UI-R-A1-ev-b-12-0-UI 5', mRNA sequence.
ACCESSION   BF556958
VERSION     BF556958.1
DBLINK      BioSample: SAMN00155772
KEYWORDS    EST.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Bonaldo,M.F., Lennon,G. and Soares,M.B.
  TITLE     Normalization and subtraction: two approaches to facilitate gene
            discovery
  JOURNAL   Genome Res. 6 (9), 791-806 (1996)
   PUBMED   8889548
COMMENT     Contact: Soares, MB
            Cancer Biology & Epigenomics Program
            Children's Memorial Research Center
            2300 Children's Plaza, Box 220, Chicago, IL 60614-3394, USA
            Tel: 773 755 6551
            Fax: 773 755 6378
            Email: mbsoares@childrensmemorial.org
            cDNA Library Preparation: M.B. Soares Lab Clone distribution:
            clones will be available through Research Genetics (www.resgen.com)
            This clone is also available through the I.M.A.G.E. Consortium at
            LLNL (info@image.llnl.gov). IMAGE ID= 1771795
            Seq primer: M13 Forward.
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /clone="UI-R-A1-ev-b-12-0-UI"
                     /clone_lib="SAMN00155772 UI-R-A1"
                     /dev_stage="adult"
                     /lab_host="DH10B (Life Technologies)"
                     /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI;
                     The UI-R-A1 library is a subtracted library derived from
                     the UI-R-A0 library. The UI-R-A0 library consisted of a
                     mixture of individually tagged normalized libraries
                     constructed from rat placenta, adult lung, brain, liver,
                     kidney, heart, spleen, ovary, and muscle. The tag is a
                     string of 3-5 nucleotides present between the Not I site
                     and the oligo-dT track which allows identification of the
                     library of origin of a clone within the mixture. The
                     subtracted library (UI-R-A1) was constructed as follows:
                     PCR amplified cDNA inserts from a pool of approximately
                     3,840 UI-R-A0 clones from which 3' ESTs had been derived
                     was used as a driver in a hybridization with the UI-R-A0
                     library in the form of single-stranded circles. The
                     remaining single-stranded circles (subtracted library) was
                     purified by hydroxyapatite column chromatography,
                     converted to double-stranded circles and electroporated
                     into DH10B bacteria (Life Technologies) to generate the
                     UI-R-A1 library. This procedure has been previously
                     described (Bonaldo, Lennon and Soares, Genome Research 6:
                     791-806, 1996)"
ORIGIN      
        1 cacgaggtgt ggttacattg gtgccttact ggctatcagg agacagtatc acagcattgt
       61 tccagcactt tatgagtaca caagtcagct attaaaacgt cgagtggtgt cagtaccctt
      121 gaaaattgag catctctctg aggaactgga tgcatggaga gcttgcacac agtccaccaa
      181 acaattgtca gaagattctc catatgtacc cccttctgat tcacaaagaa cggtttatga
      241 aactttatta aagagactaa aagaagagcc gctcaaagga attactggac cagattatgt
      301 gactggaaca aatcttccaa gccattcaga cattcatatt tcttgtctta atggattaaa
      361 aatccagggt cctgtgtatt ttctggaaga tgggaaatca gctatttctt tgaatgatgc
      421 tctgatgtgg gcaaaggtga atccattctc acctttaaga actggaatac gcctcaatcc
      481 attttgactg aagatttttc tccacactta gtagtttttg taaaaaggaa atttggggta
      541 tttattacct tcattttggt tgtccaaaaa caata
//
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