LOCUS BF492436 619 bp mRNA linear EST 23-APR-2001
DEFINITION AT29378.3prime AT Drosophila melanogaster adult testes pOTB7
Drosophila melanogaster cDNA clone AT29378 3 similar to ptr:
FBan0002841 located on: X 2F4-2F4;: 04/09/2001, mRNA sequence.
ACCESSION BF492436
VERSION BF492436.1
DBLINK BioSample: SAMN00161767
KEYWORDS EST.
SOURCE Drosophila melanogaster (fruit fly)
ORGANISM Drosophila melanogaster
Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
Pterygota; Neoptera; Endopterygota; Diptera; Brachycera;
Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora.
REFERENCE 1 (bases 1 to 619)
AUTHORS Stapleton,M., Brokstein,P., Hong,L., Agbayani,A., Baxter,E.,
Berman,B., Carlson,J., Champe,M., Chavez,C., Chew,M., Dorsett,V.,
Farfan,D., Frise,E., George,R., Gonzalez,M., Guarin,H., Harris,N.,
Li,P., Liao,G., Miranda,A., Misra,S., Mungall,C.J., Nunoo,J.,
Pacleb,J., Paragas,V., Park,S., Phouanenavong,S., Wan,K., Yu,C.,
Lewis,S.E., Celniker,S. and Rubin,G.M.
TITLE BDGP/HHMI AT Drosophila EST Project
JOURNAL Unpublished
COMMENT Contact: Carlson, J.
BDGP
Lawrence Berkeley National Lab
One Cyclotron Rd, Berkeley, CA 94720, USA
Fax: 510 486 7571
Email: http://www.fruitfly.org/EST/index.shtml, cdna@fruitfly.org
Based upon the presence of a XhoI site followed by a run of 14 or
more T residues at the beginning of the sequence, this clone
probably contains an inverted insert. The resulting Poly-T sequence
has been removed. hit genomic AE003423: arm:X [1813638,2105613]
estimated-cyto:2C6-2F5: 04/09/2001
Plate: AT.293 row: G column: 6
High quality sequence stop: 550.
FEATURES Location/Qualifiers
source 1..619
/organism="Drosophila melanogaster"
/mol_type="mRNA"
/db_xref="taxon:7227"
/clone="AT29378"
/sex="male"
/clone_lib="SAMN00161767 AT Drosophila melanogaster adult
testes pOTB7"
/dev_stage="0-3 day old Ore-R males"
/lab_host="Plates AT.10-AT.120: DH5-alpha. Plates
AT.121-AT.319: DH5-alpha TonA"
/note="Organ: ADULT testes; Vector: pOTB7; Site_1: EcoRI;
Site_2: Xho1; The mRNA for the testis library was made
from testes and seminal vessicles hand dissected from 0-3
day old Ore-R males. RNA kindly provided by the lab of
Margaret Fuller. Sized fractionated cDNAs were directly
ligated into pOTB7. Plasmid cDNA library."
ORIGIN
1 gaggtgaaaa taagttttac taaatcttgg ctttatcgaa attatataca aactcaacac
61 aaaatgtatt gttaacggtt taaaaaatat ataacttgtg cgctgcttca ataatttttg
121 cccgactctc ctcagttcac tttcaaaaac ttaggatcgg ttgaggtcct cgcgtttgaa
181 gttccgtatc gcgtccaaca gttgggagcg cggatcaggc gcggtgggac cactgcgtcc
241 gccgttggtt ttcacgggct gcagtgccgg aggcggcggc ggaggggagg gtgtggtcgc
301 tggctggaca gtgggcttga gtttgacctg caggctcacc ggcttagttt ctggctcctg
361 gagctttacc tccactttgg tggacttcag agcgacgttc ccactttttg acggcgttgc
421 tggtttgctt tctggcgggg acttgggcgc ggtagctgaa gaaggaatgc tttcgttcac
481 ggacaccctt attctcaagg cctgctcctg ttcctgctgc tccttggcca acatacgttc
541 cttgaagcct gtgcggcgta aagtactctg gccaaagggc gctggtgcag gattggcttc
601 cactggtctg ttcctcttc
//