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GenBank: AW450733.1
FASTA Graphics
LOCUS AW450733 376 bp mRNA linear EST 07-JAN-2011 DEFINITION UI-H-BI3-akq-e-02-0-UI.s1 NCI_CGAP_Sub5 Homo sapiens cDNA clone IMAGE:2735306 3', mRNA sequence. ACCESSION AW450733 VERSION AW450733.1 DBLINK BioSample: SAMN00157374 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 376) CONSRTM NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov The sequence contained an oligo-dT track that was present in the oligonucleotide that was used to prime the synthesis of first strand cDNA and therefore this may represent a bonafide poly A tail. cDNA Library Preparation: M.B. Soares Lab Clone distribution: NCI-CGAP clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: www-bio.llnl.gov/bbrp/image/image.html Seq primer: M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..376 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="IMAGE:2735306" /clone_lib="SAMN00157374 NCI_CGAP_Sub5" /lab_host="DH10B (Life Technologies)" /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI; NCI_CGAP_Sub5 is a subtracted library derived from NCI_CGAP_Sub4. The NCI_CGAP_Sub5 library had 3 million recombinants. A single-stranded DNA preparation of NCI_CGAP_Sub4 was used as a tracer in a subtractive hybridization with a driver comprising: the IMAGE pool (NCI_CGAP_Kid3 pool 1 LLAM 3334-3337, 3682-3683, 3798-3803 (IMAGE CloneIDs 1322376-1323911, 1456008-1456775,1500552-1502855); NCI_CGAP_Kid5 pool 1 LLAM 3338-3342,3722-3725, 3776-3778 (IMAGE CloneIDs 1323912-1325831, 1471368-1472903, 1492104-1493255); NCI_CGAP_Lu5 pool 1 LLAM 3575-3582, 3851-3854 (IMAGE CloneIDs 1414920-1417991,1520904-1522439); NCI_CGAP_GC4 pool 1 LLAM 3164-3167, 3716-3720, 3733-3735 (IMAGE CloneIDs 1257096-1258631,1469064-1470983, 1475592-1476743); NCI_CGAP_Pr22 pool 1 LLAM 2457-2459, 2758-2759, 3062-3068 (IMAGE CloneIDs 985608-986759,1101192-1101959, 1217928-1220615); NCI_CGAP_Co10 pool 1 LLAM 2644-2653, 2871-2872 (IMAGE CloneIDs 1057416-1061255,1144584-1145351). (10% of the driver population), plus a pool of 3,840 arrayed clones from NCI_CGAP_Sub1 (IMAGE CloneIDs 2708616-2710535) and NCI_CGAP_Sub2 (IMAGE CloneIDs 2710536-2712455) (10% of the driver population), plus a pool of 11,136 clones from NCI_CGAP_Sub3 (IMAGE CloneIDs 2712456-2723591) (10% of the driver population), plus a pool of 5,472 clones from NCI_CGAP_Sub4 (IMAGE CloneIDs 2723592-2728969) (70% of the driver population). Subtraction was performed as previously described [Bonaldo, Lennon & Soares (1996): Normalization and Subtraction: Two Approaches To Facilitate Gene Discovery. Genome Research 6, 791-806. TAG_LIB=NCI_CGAP_Lu19; TAG_SEQ=GACAGC; TAG_TISSUE=lung" ORIGIN 1 tttttttttt ttcaatattt atccacaggt cttgacattt accatctcag ggaggttgat 61 atttccactg attcatgcgg tagaagcaat ggctcatgga tctctaagtc tggagaccag 121 cttggctgtc ttaacagggc tcccccattt ctcagagaaa gaattataac caggctatat 181 gtgcaggctg tgtgagccca gaaaatgaca gttactgctt tccaggaatg ggaataaaca 241 gatattcact gtactctcct ggaaaaactg aggttgccta ggggtcagtt ttgaatcagg 301 caatccaata tttgagcttt ttgccagaga aacaaaatga gacattgata tgctaagaag 361 ttgctaactg agtcag //
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