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UI-H-BI3-akq-e-02-0-UI.s1 NCI_CGAP_Sub5 Homo sapiens cDNA clone IMAGE:2735306 3', mRNA sequence

GenBank: AW450733.1

FASTA Graphics 

LOCUS       AW450733                 376 bp    mRNA    linear   EST 07-JAN-2011
DEFINITION  UI-H-BI3-akq-e-02-0-UI.s1 NCI_CGAP_Sub5 Homo sapiens cDNA clone
            IMAGE:2735306 3', mRNA sequence.
ACCESSION   AW450733
VERSION     AW450733.1
DBLINK      BioSample: SAMN00157374
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 376)
  CONSRTM   NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap
  TITLE     National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
            Tumor Gene Index
  JOURNAL   Unpublished
COMMENT     Contact: Robert Strausberg, Ph.D.
            Email: cgapbs-r@mail.nih.gov
            The sequence contained an oligo-dT track that was present in the
            oligonucleotide that was used to prime the synthesis of first
            strand cDNA and therefore this may represent a bonafide poly A
            tail. cDNA Library Preparation: M.B. Soares Lab Clone distribution:
            NCI-CGAP clone distribution information can be found through the
            I.M.A.G.E. Consortium/LLNL at:
            www-bio.llnl.gov/bbrp/image/image.html
            Seq primer: M13 Forward
            POLYA=Yes.
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:2735306"
                     /clone_lib="SAMN00157374 NCI_CGAP_Sub5"
                     /lab_host="DH10B (Life Technologies)"
                     /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI;
                     NCI_CGAP_Sub5 is a subtracted library derived from
                     NCI_CGAP_Sub4. The NCI_CGAP_Sub5 library had 3 million
                     recombinants. A single-stranded DNA preparation of
                     NCI_CGAP_Sub4 was used as a tracer in a subtractive
                     hybridization with a driver comprising: the IMAGE pool
                     (NCI_CGAP_Kid3 pool 1 LLAM 3334-3337, 3682-3683, 3798-3803
                     (IMAGE CloneIDs 1322376-1323911,
                     1456008-1456775,1500552-1502855); NCI_CGAP_Kid5 pool 1
                     LLAM 3338-3342,3722-3725, 3776-3778 (IMAGE CloneIDs
                     1323912-1325831, 1471368-1472903, 1492104-1493255);
                     NCI_CGAP_Lu5 pool 1 LLAM 3575-3582, 3851-3854 (IMAGE
                     CloneIDs 1414920-1417991,1520904-1522439); NCI_CGAP_GC4
                     pool 1 LLAM 3164-3167, 3716-3720, 3733-3735 (IMAGE
                     CloneIDs 1257096-1258631,1469064-1470983,
                     1475592-1476743); NCI_CGAP_Pr22 pool 1 LLAM 2457-2459,
                     2758-2759, 3062-3068 (IMAGE CloneIDs
                     985608-986759,1101192-1101959, 1217928-1220615);
                     NCI_CGAP_Co10 pool 1 LLAM 2644-2653, 2871-2872 (IMAGE
                     CloneIDs 1057416-1061255,1144584-1145351). (10% of the
                     driver population), plus a pool of 3,840 arrayed clones
                     from NCI_CGAP_Sub1 (IMAGE CloneIDs 2708616-2710535) and
                     NCI_CGAP_Sub2 (IMAGE CloneIDs 2710536-2712455) (10% of the
                     driver population), plus a pool of 11,136 clones from
                     NCI_CGAP_Sub3 (IMAGE CloneIDs 2712456-2723591) (10% of the
                     driver population), plus a pool of 5,472 clones from
                     NCI_CGAP_Sub4 (IMAGE CloneIDs 2723592-2728969) (70% of the
                     driver population). Subtraction was performed as
                     previously described [Bonaldo, Lennon & Soares (1996):
                     Normalization and Subtraction: Two Approaches To
                     Facilitate Gene Discovery. Genome Research 6, 791-806.
                     TAG_LIB=NCI_CGAP_Lu19;
                     TAG_SEQ=GACAGC;
                     TAG_TISSUE=lung"
ORIGIN      
        1 tttttttttt ttcaatattt atccacaggt cttgacattt accatctcag ggaggttgat
       61 atttccactg attcatgcgg tagaagcaat ggctcatgga tctctaagtc tggagaccag
      121 cttggctgtc ttaacagggc tcccccattt ctcagagaaa gaattataac caggctatat
      181 gtgcaggctg tgtgagccca gaaaatgaca gttactgctt tccaggaatg ggaataaaca
      241 gatattcact gtactctcct ggaaaaactg aggttgccta ggggtcagtt ttgaatcagg
      301 caatccaata tttgagcttt ttgccagaga aacaaaatga gacattgata tgctaagaag
      361 ttgctaactg agtcag
//
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