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qf60c07.x1 Soares_testis_NHT Homo sapiens cDNA clone IMAGE:1754412 3', mRNA sequence

GenBank: AI205913.1

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LOCUS       AI205913                 300 bp    mRNA    linear   EST 05-JAN-2011
DEFINITION  qf60c07.x1 Soares_testis_NHT Homo sapiens cDNA clone IMAGE:1754412
            3', mRNA sequence.
ACCESSION   AI205913
VERSION     AI205913.1
DBLINK      BioSample: SAMN00155229
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 300)
  CONSRTM   NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap
  TITLE     National Cancer Institute, Cancer Genome Anatomy Project (CGAP),
            Tumor Gene Index
  JOURNAL   Unpublished
COMMENT     Contact: Robert Strausberg, Ph.D.
            Email: cgapbs-r@mail.nih.gov
            cDNA Library Preparation: M. Bento Soares, Ph.D., M. Fatima
            Bonaldo, Ph.D.
             cDNA Library Arrayed by: Greg Lennon, Ph.D.
             DNA Sequencing by: Washington University Genome Sequencing Center
             Clone distribution: NCI-CGAP clone distribution information can be
            found through the I.M.A.G.E. Consortium/LLNL at:
            www-bio.llnl.gov/bbrp/image/image.html
            
            Seq primer: -40UP from Gibco
            High quality sequence stop: 278.
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:1754412"
                     /sex="male"
                     /clone_lib="SAMN00155229 Soares_testis_NHT"
                     /lab_host="DH10B"
                     /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI;
                     1st strand cDNA was prepared from mRNA obtained from
                     Clontech Laboratories, Inc., and primed with a Not I -
                     oligo(dT) primer [5'
                     TGTTACCAATCTGAAGTGGGAGCGGCCGCCCAATTTTTTTTTTTTTTTTT 3'].
                     Double-stranded cDNA was ligated to Eco RI adaptors
                     (Pharmacia), digested with Not I and cloned into the Not I
                     and Eco RI sites of the modified pT7T3 vector. Library
                     went through one round of normalization to Cot5, and was
                     constructed by Bento Soares and M. Fatima Bonaldo."
ORIGIN      
        1 tttttaacat ttcaaacatc tactatttat taattcccca tagccatttg atcgccaaag
       61 acagttatta ctttttaagt ttctcttaca tccccatact actttgcttc tgtaccatca
      121 acatcctggc ccaaactacc aacatagttc tcttggaatt ttgtaaaagt ttttataact
      181 attccaacta caacagtgcc ttctataata ggaaaaaaaa agtgaaagtt cttagtcctc
      241 aaatctgtga aggaagcata gtaaagtctt aagnttagtt tcccaagtgc ctgtagaaat
//
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