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zh50d01.r1 Soares_fetal_liver_spleen_1NFLS_S1 Homo sapiens cDNA clone IMAGE:415489 5', mRNA sequence

GenBank: W78961.1

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LOCUS       W78961                   469 bp    mRNA    linear   EST 28-JAN-2011
DEFINITION  zh50d01.r1 Soares_fetal_liver_spleen_1NFLS_S1 Homo sapiens cDNA
            clone IMAGE:415489 5', mRNA sequence.
ACCESSION   W78961
VERSION     W78961.1
DBLINK      BioSample: SAMN00155062
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Hillier,L., Lennon,G., Becker,M., Bonaldo,M.F., Chiapelli,B.,
            Chissoe,S., Dietrich,N., DuBuque,T., Favello,A., Gish,W.,
            Hawkins,M., Hultman,M., Kucaba,T., Lacy,M., Le,M., Le,N.,
            Mardis,E., Moore,B., Morris,M., Parsons,J., Prange,C., Rifkin,L.,
            Rohlfing,T., Schellenberg,K., Soares,M.B., Tan,F., Thierry-Meg,J.,
            Trevaskis,E., Underwood,K., Wohldmann,P., Waterston,R., Wilson,R.
            and Marra,M.
  TITLE     Generation and analysis of 280,000 human expressed sequence tags
  JOURNAL   Genome Res. 6 (9), 807-828 (1996)
   PUBMED   8889549
COMMENT     Contact: Wilson RK
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: est@watson.wustl.edu
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            Insert Length: 1211   Std Error: 0.00
            Seq primer: mob.REGA+ET
            High quality sequence stop: 280.
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="GDB:1323959"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:415489"
                     /sex="male"
                     /clone_lib="SAMN00155062
                     Soares_fetal_liver_spleen_1NFLS_S1"
                     /dev_stage="20 week-post conception fetus"
                     /lab_host="DH10B (ampicillin resistant)"
                     /note="Organ: Liver and Spleen; Vector: pT7T3D (Pharmacia)
                     with a modified polylinker; Site_1: Pac I; Site_2: Eco RI;
                     This is a subtracted version of the original Soares fetal
                     liver spleen 1NFLS library. 1st strand cDNA was primed
                     with a Pac I - oligo(dT) primer [5'
                     AACTGGAAGAATTAATTAAAGATCTTTTTTTTTTTTTTTTTTT 3'],
                     double-stranded cDNA was ligated to Eco RI adaptors
                     (Pharmacia), digested with Pac I and cloned into the Pac I
                     and Eco RI sites of the modified pT7T3 vector. Library
                     went through one round of normalization. Library
                     constructed by Bento Soares and M.Fatima Bonaldo."
ORIGIN      
        1 tnttcttttt tttttgagac agaatttcct cttgttgccc aggctggagt gcaatggtgc
       61 gatcttggct tgccgaggga taataggtcc aaatatactt gaccataaat tctttagtaa
      121 gatgaagctg tctctagttg ttatcctcac agtgtcaaca ttgaaccatg acttctgaga
      181 ttcatcttta atgctgaata cgtacttgac ttgctttttt tcttcagttg aatttctagc
      241 atccctttta ccaattcatt tattggctca gcttgttaat tctaatttgc ctttgactgc
      301 tcagacgtgg cttgaaagac ttgagcattg ttctgatgtt gagggaatat ttgctttttc
      361 tgtaatgact gcagatcaga tgggagctcn ttttacacag ggactttgag ggctcaggnt
      421 catcaaagaa gttcagtaat ggatggtggt acaagaacca cagggcttn
//
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