LOCUS W78961 469 bp mRNA linear EST 28-JAN-2011
DEFINITION zh50d01.r1 Soares_fetal_liver_spleen_1NFLS_S1 Homo sapiens cDNA
clone IMAGE:415489 5', mRNA sequence.
ACCESSION W78961
VERSION W78961.1
DBLINK BioSample: SAMN00155062
KEYWORDS EST.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 469)
AUTHORS Hillier,L., Lennon,G., Becker,M., Bonaldo,M.F., Chiapelli,B.,
Chissoe,S., Dietrich,N., DuBuque,T., Favello,A., Gish,W.,
Hawkins,M., Hultman,M., Kucaba,T., Lacy,M., Le,M., Le,N.,
Mardis,E., Moore,B., Morris,M., Parsons,J., Prange,C., Rifkin,L.,
Rohlfing,T., Schellenberg,K., Soares,M.B., Tan,F., Thierry-Meg,J.,
Trevaskis,E., Underwood,K., Wohldmann,P., Waterston,R., Wilson,R.
and Marra,M.
TITLE Generation and analysis of 280,000 human expressed sequence tags
JOURNAL Genome Res. 6 (9), 807-828 (1996)
PUBMED 8889549
COMMENT Contact: Wilson RK
Washington University School of Medicine
4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
Tel: 314 286 1800
Fax: 314 286 1810
Email: est@watson.wustl.edu
This clone is available royalty-free through LLNL ; contact the
IMAGE Consortium (info@image.llnl.gov) for further information.
Insert Length: 1211 Std Error: 0.00
Seq primer: mob.REGA+ET
High quality sequence stop: 280.
FEATURES Location/Qualifiers
source 1..469
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="GDB:1323959"
/db_xref="taxon:9606"
/clone="IMAGE:415489"
/sex="male"
/clone_lib="SAMN00155062
Soares_fetal_liver_spleen_1NFLS_S1"
/dev_stage="20 week-post conception fetus"
/lab_host="DH10B (ampicillin resistant)"
/note="Organ: Liver and Spleen; Vector: pT7T3D (Pharmacia)
with a modified polylinker; Site_1: Pac I; Site_2: Eco RI;
This is a subtracted version of the original Soares fetal
liver spleen 1NFLS library. 1st strand cDNA was primed
with a Pac I - oligo(dT) primer [5'
AACTGGAAGAATTAATTAAAGATCTTTTTTTTTTTTTTTTTTT 3'],
double-stranded cDNA was ligated to Eco RI adaptors
(Pharmacia), digested with Pac I and cloned into the Pac I
and Eco RI sites of the modified pT7T3 vector. Library
went through one round of normalization. Library
constructed by Bento Soares and M.Fatima Bonaldo."
ORIGIN
1 tnttcttttt tttttgagac agaatttcct cttgttgccc aggctggagt gcaatggtgc
61 gatcttggct tgccgaggga taataggtcc aaatatactt gaccataaat tctttagtaa
121 gatgaagctg tctctagttg ttatcctcac agtgtcaaca ttgaaccatg acttctgaga
181 ttcatcttta atgctgaata cgtacttgac ttgctttttt tcttcagttg aatttctagc
241 atccctttta ccaattcatt tattggctca gcttgttaat tctaatttgc ctttgactgc
301 tcagacgtgg cttgaaagac ttgagcattg ttctgatgtt gagggaatat ttgctttttc
361 tgtaatgact gcagatcaga tgggagctcn ttttacacag ggactttgag ggctcaggnt
421 catcaaagaa gttcagtaat ggatggtggt acaagaacca cagggcttn
//