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ma37a12.r1 Soares mouse p3NMF19.5 Mus musculus cDNA clone IMAGE:312862 5', mRNA sequence

GenBank: W10255.1

FASTA Graphics 

LOCUS       W10255                   374 bp    mRNA    linear   EST 01-OCT-1997
DEFINITION  ma37a12.r1 Soares mouse p3NMF19.5 Mus musculus cDNA clone
            IMAGE:312862 5', mRNA sequence.
ACCESSION   W10255
VERSION     W10255.1
DBLINK      BioSample: SAMN00155042
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Marra,M., Hillier,L., Allen,M., Bowles,M., Dietrich,N., Dubuque,T.,
            Geisel,S., Kucaba,T., Lacy,M., Le,M., Martin,J., Morris,M.,
            Schellenberg,K., Steptoe,M., Tan,F., Underwood,K., Moore,B.,
            Theising,B., Wylie,T., Lennon,G., Soares,B., Wilson,R. and
            Waterston,R.
  TITLE     The WashU-HHMI Mouse EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Marra M/Mouse EST Project
            WashU-HHMI Mouse EST Project
            Washington University School of MedicineP
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: mouseest@watson.wustl.edu
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            MGI: 203478
            Seq primer: ETPrimer
            High quality sequence stop: 372.
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /clone="IMAGE:312862"
                     /clone_lib="SAMN00155042 Soares mouse p3NMF19.5"
                     /dev_stage="19.5 dpc total fetus"
                     /lab_host="DH10B (ampicillin resistant)"
                     /note="Vector: pT7T3D (Pharmacia) with a modified
                     polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA
                     was primed with a Not I - oligo(dT) primer [5'
                     TGTTACCAATCTGAAGTGGGAGCGGCCGCATTTTTTTTTTTTTTTTTTTT 3'],
                     double-stranded cDNA was size selected, ligated to Eco RI
                     adapters (Pharmacia), digested with Not I and cloned into
                     the Not I and Eco RI sites of a modified pT7T3 vector
                     (Pharmacia). Library went through one round of
                     normalization to a Cot = 5. Library constructed by Bento
                     Soares and M.Fatima Bonaldo. RNA was kindly provided by
                     Dr. Minoru Ko (Wayne State University)."
ORIGIN      
        1 aaggacattg agctgtctga cgatccttat gactgcatcc gactgagtgt agagaatgtc
       61 ccctgcattg tcaccctgtg caagattggc tgccggcatg tggtagatgc cacacttcaa
      121 gaggaggcct gttccctggc cagcttgctg gtgtcagtga ccagcaaggg agtagtgaca
      181 tgcatgagga aagtggggaa aggaagcctg gatcctgaga gcatcttcga gatgatggag
      241 agcagcaagc gagtgggcaa ggtgctgcac gtgtccttgc agagccttct gcacaaggaa
      301 gaaagcctgg ggcccaagag gccgagagtc gggttcctgg ggtgatttct ctcactgcac
      361 gttggttgcc tcac
//
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