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GenBank: W10255.1
FASTA Graphics
LOCUS W10255 374 bp mRNA linear EST 01-OCT-1997 DEFINITION ma37a12.r1 Soares mouse p3NMF19.5 Mus musculus cDNA clone IMAGE:312862 5', mRNA sequence. ACCESSION W10255 VERSION W10255.1 DBLINK BioSample: SAMN00155042 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 374) AUTHORS Marra,M., Hillier,L., Allen,M., Bowles,M., Dietrich,N., Dubuque,T., Geisel,S., Kucaba,T., Lacy,M., Le,M., Martin,J., Morris,M., Schellenberg,K., Steptoe,M., Tan,F., Underwood,K., Moore,B., Theising,B., Wylie,T., Lennon,G., Soares,B., Wilson,R. and Waterston,R. TITLE The WashU-HHMI Mouse EST Project JOURNAL Unpublished COMMENT Contact: Marra M/Mouse EST Project WashU-HHMI Mouse EST Project Washington University School of MedicineP 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108 Tel: 314 286 1800 Fax: 314 286 1810 Email: mouseest@watson.wustl.edu This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. MGI: 203478 Seq primer: ETPrimer High quality sequence stop: 372. FEATURES Location/Qualifiers source 1..374 /organism="Mus musculus" /mol_type="mRNA" /db_xref="taxon:10090" /clone="IMAGE:312862" /clone_lib="SAMN00155042 Soares mouse p3NMF19.5" /dev_stage="19.5 dpc total fetus" /lab_host="DH10B (ampicillin resistant)" /note="Vector: pT7T3D (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCATTTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was size selected, ligated to Eco RI adapters (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of a modified pT7T3 vector (Pharmacia). Library went through one round of normalization to a Cot = 5. Library constructed by Bento Soares and M.Fatima Bonaldo. RNA was kindly provided by Dr. Minoru Ko (Wayne State University)." ORIGIN 1 aaggacattg agctgtctga cgatccttat gactgcatcc gactgagtgt agagaatgtc 61 ccctgcattg tcaccctgtg caagattggc tgccggcatg tggtagatgc cacacttcaa 121 gaggaggcct gttccctggc cagcttgctg gtgtcagtga ccagcaaggg agtagtgaca 181 tgcatgagga aagtggggaa aggaagcctg gatcctgaga gcatcttcga gatgatggag 241 agcagcaagc gagtgggcaa ggtgctgcac gtgtccttgc agagccttct gcacaaggaa 301 gaaagcctgg ggcccaagag gccgagagtc gggttcctgg ggtgatttct ctcactgcac 361 gttggttgcc tcac //
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