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GenBank: T21620.1
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LOCUS T21620 466 bp mRNA linear EST 28-JAN-2011 DEFINITION 3628 Lambda-PRL2 Arabidopsis thaliana cDNA clone 95L17T7, mRNA sequence. ACCESSION T21620 VERSION T21620.1 DBLINK BioSample: SAMN00154689 KEYWORDS EST. SOURCE Arabidopsis thaliana (thale cress) ORGANISM Arabidopsis thaliana Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis. REFERENCE 1 (bases 1 to 466) AUTHORS Newman,T., deBruijn,F.J., Green,P., Keegstra,K., Kende,H., McIntosh,L., Ohlrogge,J., Raikhel,N., Somerville,S., Thomashow,M., Retzel,E. and Somerville,C. TITLE Genes galore: a summary of methods for accessing results from large-scale partial sequencing of anonymous Arabidopsis cDNA clones JOURNAL Plant Physiol. 106, 1241-1255 (1994) PUBMED 7846151 COMMENT On Jan 7, 1998 this sequence version replaced gi:914538. Contact: Thomas Newman MSU-DOE Plant Research Laboratory Michigan State University MSU-DOE-PRL, Michigan State University,Plant Biology Bldg.,E. Lansing,Mi Tel: 517-353-0854 Fax: 517-353-9168 Email: 22313tcn@ibm.cl.msu.edu Seq primer: T7. FEATURES Location/Qualifiers source 1..466 /organism="Arabidopsis thaliana" /mol_type="mRNA" /db_xref="taxon:3702" /clone="95L17T7" /clone_lib="SAMN00154689 Lambda-PRL2" /ecotype="Columbia" /note="Vector: lambda Zip-Lox; Site_1: Sal; Site_2: Not; Lambda PRL2 is a cDNA library derived from equal quantities of 4 pools of mRNA. The mRNA sources were 1) 7 day germinated etiolated seedlings; 2) tissue culture grown roots; 3) staged plants half with 24 hour light cycle, half on 16 hr light, 8 hour dark- rosettes; 4) same plants as 3 but aerial tissue (stems, flowers and siliques. The vector is BRL's lambda Zip-Lox. The cDNA inserts were directionally cloned with Sal-Not arms using oligo dT primed cDNA." ORIGIN 1 gaagaaaaga acagagaaaa cagaggaaga ggtgagatgg aggaacttgt agggcttcta 61 agaatccgag tgaagagagg gatcaatctt nctcagcgag acactctaag cagcgaccct 121 tttnttgtca taaccatggg atcacagaag ctgaagactc gtnttgtgga aaataactgc 181 aaccctgagt ggaacgagga attaaccctt gcnttaagac atcccgatga acctgtgaat 241 ctaatagtnt atgataaaga tacattcaca tcgcacgtca agatgggaga tgcaaagata 301 ggtattaaac catttntggn aggttcacaa aattggggtt ttcnaagacc ttcccagntt 361 gggaccaggn gntcaaaaga gnagnttttt gcccccacca ggggggaaan ctttttttgg 421 ctttaagcng agncagtttt cggcttccca taanttgggc aaanat //
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